Dispersion-free inertial focusing (DIF) for high-yield polydisperse micro-particles filtration and analysis DOI Creative Commons
Kelvin C. M. Lee,

Bob M. F. Chung,

Dickson M. D. Siu

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 23, 2024

Abstract Inertial focusing excels at the precise spatial ordering and separation of microparticles by size within fluid flows. However, this advantage, brought its inherent size-dependent dispersion, could turn into a drawback that challenges applications requiring consistent uniform positioning polydisperse particles, such as microfiltration flow cytometry. To overcome fundamental challenge, we introduce Dispersion-Free Focusing (DIF). This new method minimizes particle dispersion while maintaining high throughput precision standard inertial focusing, even in highly scenario. We demonstrate rule-of-thumb principle to reinvent system achieve an efficient particles ranging from 6 30 µm diameter onto single plane with less than 3 variance over 95% efficiency scalable (2.4-30 mL/hr) – stark contrast existing technologies struggle polydispersity. demonstrated DIF be applied broad range applications, particularly enabling high-yield continuous microparticle filtration large-scale high-resolution single-cell morphological analysis heterogeneous cell populations. technique is also readily compatible microfluidic design thus unleash more diverse systems applications.

Язык: Английский

Modular microfluidics for life sciences DOI Creative Commons
Jialin Wu, Hui Fang, Jun Zhang

и другие.

Journal of Nanobiotechnology, Год журнала: 2023, Номер 21(1)

Опубликована: Март 11, 2023

The advancement of microfluidics has enabled numerous discoveries and technologies in life sciences. However, due to the lack industry standards configurability, design fabrication microfluidic devices require highly skilled technicians. diversity discourages biologists chemists from applying this technique their laboratories. Modular microfluidics, which integrates standardized modules into a whole, complex platform, brings capability configurability conventional microfluidics. exciting features, including portability, on-site deployability, high customization motivate us review state-of-the-art modular discuss future perspectives. In review, we first introduce working mechanisms basic evaluate feasibility as components. Next, explain connection approaches among these modules, summarize advantages over integrated biological applications. Finally, challenge perspectives

Язык: Английский

Процитировано

49
Untethered Small-Scale Machines for Microrobotic Manipulation: From Individual and Multiple to Collective Machines DOI
Qianqian Wang, Jiachen Zhang, Jiangfan Yu

и другие.

ACS Nano, Год журнала: 2023, Номер 17(14), С. 13081 - 13109

Опубликована: Июль 13, 2023

Untethered small-scale machines (USSMs) that can actively adjust their motion, deformation, and collective states in response to external stimuli have gained enormous interest various manipulation, sensing, biomedical applications. Because they be efficiently operated confined tortuous environments, USSMs are capable of conducting wireless microrobotic manipulation tasks tethered find hard achieve. Over the past decade development, significant research progress has been achieved designing USSM-based strategies, which enabled by investigating machine-object, machine-environment, machine-machine interactions. This review summarizes latest developments for utilizing individual machines, coordinating multiple inducing behaviors. Providing recent studies relevant applications areas, we also discuss challenges future perspectives facing USSMs-based intelligent systems achieve complex environments with imaging-guided processes increasing autonomy levels.

Язык: Английский

Процитировано

33
Recent Developments in Inertial and Centrifugal Microfluidic Systems along with the Involved Forces for Cancer Cell Separation: A Review DOI Creative Commons
A. Farahinia, Wenjun Zhang, Ildikó Badea

и другие.

Sensors, Год журнала: 2023, Номер 23(11), С. 5300 - 5300

Опубликована: Июнь 2, 2023

The treatment of cancers is a significant challenge in the healthcare context today. Spreading circulating tumor cells (CTCs) throughout body will eventually lead to cancer metastasis and produce new tumors near healthy tissues. Therefore, separating these invading extracting cues from them extremely important for determining rate progression inside development individualized treatments, especially at beginning process. continuous fast separation CTCs has recently been achieved using numerous techniques, some which involve multiple high-level operational protocols. Although simple blood test can detect presence circulation system, detection still restricted due scarcity heterogeneity CTCs. more reliable effective techniques thus highly desired. technology microfluidic devices promising among many other bio-chemical bio-physical technologies. This paper reviews recent developments two types devices, are based on size and/or density cells, cells. goal this review identify knowledge or gaps suggest future works.

Язык: Английский

Процитировано

28
Droplet Microfluidics Powered Hydrogel Microparticles for Stem Cell‐Mediated Biomedical Applications DOI

Fangqiao Zheng,

Ruizhi Tian,

Hongxu Lu

и другие.

Small, Год журнала: 2024, Номер unknown

Опубликована: Июнь 16, 2024

Abstract Stem cell‐related therapeutic technologies have garnered significant attention of the research community for their multi‐faceted applications. To promote effects stem cells, strategies cell microencapsulation in hydrogel microparticles been widely explored, as potential to facilitate oxygen diffusion and nutrient transport alongside ability crucial cell‐cell cell‐matrix interactions. Despite promise, there is an acute shortage automated, standardized, reproducible platforms further research. Microfluidics offers intriguing platform produce cell‐laden (SCHMs) owing its manipulate fluids at micrometer scale well precisely control structure composition microparticles. In this review, typical biomaterials crosslinking methods microfluidic encapsulation cells progress droplet‐based microfluidics fabrication SCHMs are outlined. Moreover, important biomedical applications highlighted, including regenerative medicine, tissue engineering, scale‐up production microenvironmental simulation fundamental studies. Overall, holds tremendous enabling diverse worthy various

Язык: Английский

Процитировано

14
Recent progress of nanostructure-based enrichment of circulating tumor cells and downstream analysis DOI Open Access
Lihua Guo, Chang Liu, Manlin Qi

и другие.

Lab on a Chip, Год журнала: 2023, Номер 23(6), С. 1493 - 1523

Опубликована: Янв. 1, 2023

This review introduces various in vitro and vivo CTC enrichment technologies based on the characteristics of nanostructures provides detailed information about clinical applications CTC.

Язык: Английский

Процитировано

21
High‐Throughput Sorting and Single‐Cell Mechanotyping by Hydrodynamic Sorting‐Mechanotyping Cytometry DOI
Chen Yao,

Chen Ni,

Xiaozhe Zhang

и другие.

Small Methods, Год журнала: 2024, Номер 8(7)

Опубликована: Янв. 11, 2024

Abstract The existence of many background blood cells hinders the accurate identification circulating tumor (CTCs) in cancer patients. To unlock this limitation, a hydrodynamic sorting‐mechanotyping cytometry (HSMC) integrated with sorting‐concentration chip and detection is proposed for simultaneously achieving high‐throughput cell sorting multi‐parameter mechanotyping sorted cells. HSMC adopts spiral inertial microfluidics label‐free manner, allowing efficient enrichment from large Then, are concentrated by concentration unit finally passed through deformation. has high throughput can successfully reveal differences cellular mechanical properties. After characterizing optimizing single chips, white (WBCs) three types (A549, MCF‐7, MDA‐MB‐231 cells) achieved. accuracies WBCs different all larger than 94%, while highest accuracy up to 99.2%. This study envisions that will offer an avenue analysis intrinsic mechanics clinical medicine.

Язык: Английский

Процитировано

7
An integrated microfluidic chip for nucleic acid extraction and continued cdPCR detection of pathogens DOI
Yaru Huang, Zehang Gao, Cong Ma

и другие.

The Analyst, Год журнала: 2023, Номер 148(12), С. 2758 - 2766

Опубликована: Янв. 1, 2023

This paper introduces an enclosed microfluidic chip that integrates sample preparation and the chamber-based digital polymerase chain reaction (cdPCR). The of includes nucleic acid extraction purification based on magnetic beads, which adsorb acids by moving around chambers to complete reactions including lysis, washing, elution. cdPCR area consists tens thousands regularly arranged microchambers. After processes are completed, purified can be directly introduced into microchambers for amplification detection chip. performance quantification system were examined using synthetic SARS-CoV-2 plasmid templates at concentrations ranging from 101-105 copies per μL. Further on, a simulated clinical was used test system, integrated able accurately detect virus particle samples doped with interference (saliva) limit 10 could provide promising tool point-of-care testing pathogenic infections.

Язык: Английский

Процитировано

10
Next‐generation liquid biopsy instruments: Challenges and opportunities DOI Open Access
Shu Zhu,

Yaohui Fang,

Kefan Guo

и другие.

Electrophoresis, Год журнала: 2023, Номер 44(9-10), С. 775 - 783

Опубликована: Март 9, 2023

Conventional cancer diagnosis needs to excise diseased tissue from the patient's body for biopsy, causing severe injury patients. Liquid biopsy (LB), with superior advantage of minimal invasiveness, has shown its ability in real-time and been developing promising diagnostic instruments. However, until today, developed instrument still cannot be an alternative majority research clinical settings. In this paper, we first summarize challenges limitations suffered by existing LB instrument. Then, opportunities future progression next-generation are discussed detail. all, hope that can eventually integrated into workflow serve as a validated reliable tool diagnosis.

Язык: Английский

Процитировано

9
High-Throughput Microfluidics for Precise Separation and Focusing of Circulating Tumor Cells with Optimized Triangular Microchannel Design DOI

Amirreza Khodayari,

Sina Ebrahimi, Mohammadmahdi Topaheidari

и другие.

Talanta, Год журнала: 2025, Номер unknown, С. 128022 - 128022

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Efficient Extraction of White Blood Cells Using Modular Inertial Microfluidics DOI

Mingqi Yang,

Chen Ni,

Dan Wu

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 16, 2025

The existence of massive amounts red blood cells (RBCs) and other cell components poses a challenge for the efficient extraction white (WBCs) from blood. Herein, we proposed modular inertial microfluidics WBCs lysed samples. different WBC needs could be met by flexibly combining washing module concentration in microfluidics. First, respective optimal flow rates modules with channel sizes were explored. Then, based on combination modules, three modes to meet processing large-volume sample, low volume expansion, easy operation. replacement efficiency recovery rate target particles all greater than 93%. Finally, samples was tested. combined use Modes 1 2 achieved 93.1% 93.6%, while single Mode 3 97.1% 95.9%. Compared traditional centrifuges, our showed better performance reduced residual RBC fragments. More importantly, it provided an excellent free capability needs.

Язык: Английский

Процитировано

0