bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 23, 2024
Abstract
Inertial
focusing
excels
at
the
precise
spatial
ordering
and
separation
of
microparticles
by
size
within
fluid
flows.
However,
this
advantage,
brought
its
inherent
size-dependent
dispersion,
could
turn
into
a
drawback
that
challenges
applications
requiring
consistent
uniform
positioning
polydisperse
particles,
such
as
microfiltration
flow
cytometry.
To
overcome
fundamental
challenge,
we
introduce
Dispersion-Free
Focusing
(DIF).
This
new
method
minimizes
particle
dispersion
while
maintaining
high
throughput
precision
standard
inertial
focusing,
even
in
highly
scenario.
We
demonstrate
rule-of-thumb
principle
to
reinvent
system
achieve
an
efficient
particles
ranging
from
6
30
µm
diameter
onto
single
plane
with
less
than
3
variance
over
95%
efficiency
scalable
(2.4-30
mL/hr)
–
stark
contrast
existing
technologies
struggle
polydispersity.
demonstrated
DIF
be
applied
broad
range
applications,
particularly
enabling
high-yield
continuous
microparticle
filtration
large-scale
high-resolution
single-cell
morphological
analysis
heterogeneous
cell
populations.
technique
is
also
readily
compatible
microfluidic
design
thus
unleash
more
diverse
systems
applications.
Journal of Nanobiotechnology,
Год журнала:
2023,
Номер
21(1)
Опубликована: Март 11, 2023
The
advancement
of
microfluidics
has
enabled
numerous
discoveries
and
technologies
in
life
sciences.
However,
due
to
the
lack
industry
standards
configurability,
design
fabrication
microfluidic
devices
require
highly
skilled
technicians.
diversity
discourages
biologists
chemists
from
applying
this
technique
their
laboratories.
Modular
microfluidics,
which
integrates
standardized
modules
into
a
whole,
complex
platform,
brings
capability
configurability
conventional
microfluidics.
exciting
features,
including
portability,
on-site
deployability,
high
customization
motivate
us
review
state-of-the-art
modular
discuss
future
perspectives.
In
review,
we
first
introduce
working
mechanisms
basic
evaluate
feasibility
as
components.
Next,
explain
connection
approaches
among
these
modules,
summarize
advantages
over
integrated
biological
applications.
Finally,
challenge
perspectives
ACS Nano,
Год журнала:
2023,
Номер
17(14), С. 13081 - 13109
Опубликована: Июль 13, 2023
Untethered
small-scale
machines
(USSMs)
that
can
actively
adjust
their
motion,
deformation,
and
collective
states
in
response
to
external
stimuli
have
gained
enormous
interest
various
manipulation,
sensing,
biomedical
applications.
Because
they
be
efficiently
operated
confined
tortuous
environments,
USSMs
are
capable
of
conducting
wireless
microrobotic
manipulation
tasks
tethered
find
hard
achieve.
Over
the
past
decade
development,
significant
research
progress
has
been
achieved
designing
USSM-based
strategies,
which
enabled
by
investigating
machine-object,
machine-environment,
machine-machine
interactions.
This
review
summarizes
latest
developments
for
utilizing
individual
machines,
coordinating
multiple
inducing
behaviors.
Providing
recent
studies
relevant
applications
areas,
we
also
discuss
challenges
future
perspectives
facing
USSMs-based
intelligent
systems
achieve
complex
environments
with
imaging-guided
processes
increasing
autonomy
levels.
Sensors,
Год журнала:
2023,
Номер
23(11), С. 5300 - 5300
Опубликована: Июнь 2, 2023
The
treatment
of
cancers
is
a
significant
challenge
in
the
healthcare
context
today.
Spreading
circulating
tumor
cells
(CTCs)
throughout
body
will
eventually
lead
to
cancer
metastasis
and
produce
new
tumors
near
healthy
tissues.
Therefore,
separating
these
invading
extracting
cues
from
them
extremely
important
for
determining
rate
progression
inside
development
individualized
treatments,
especially
at
beginning
process.
continuous
fast
separation
CTCs
has
recently
been
achieved
using
numerous
techniques,
some
which
involve
multiple
high-level
operational
protocols.
Although
simple
blood
test
can
detect
presence
circulation
system,
detection
still
restricted
due
scarcity
heterogeneity
CTCs.
more
reliable
effective
techniques
thus
highly
desired.
technology
microfluidic
devices
promising
among
many
other
bio-chemical
bio-physical
technologies.
This
paper
reviews
recent
developments
two
types
devices,
are
based
on
size
and/or
density
cells,
cells.
goal
this
review
identify
knowledge
or
gaps
suggest
future
works.
Abstract
Stem
cell‐related
therapeutic
technologies
have
garnered
significant
attention
of
the
research
community
for
their
multi‐faceted
applications.
To
promote
effects
stem
cells,
strategies
cell
microencapsulation
in
hydrogel
microparticles
been
widely
explored,
as
potential
to
facilitate
oxygen
diffusion
and
nutrient
transport
alongside
ability
crucial
cell‐cell
cell‐matrix
interactions.
Despite
promise,
there
is
an
acute
shortage
automated,
standardized,
reproducible
platforms
further
research.
Microfluidics
offers
intriguing
platform
produce
cell‐laden
(SCHMs)
owing
its
manipulate
fluids
at
micrometer
scale
well
precisely
control
structure
composition
microparticles.
In
this
review,
typical
biomaterials
crosslinking
methods
microfluidic
encapsulation
cells
progress
droplet‐based
microfluidics
fabrication
SCHMs
are
outlined.
Moreover,
important
biomedical
applications
highlighted,
including
regenerative
medicine,
tissue
engineering,
scale‐up
production
microenvironmental
simulation
fundamental
studies.
Overall,
holds
tremendous
enabling
diverse
worthy
various
Lab on a Chip,
Год журнала:
2023,
Номер
23(6), С. 1493 - 1523
Опубликована: Янв. 1, 2023
This
review
introduces
various
in
vitro
and
vivo
CTC
enrichment
technologies
based
on
the
characteristics
of
nanostructures
provides
detailed
information
about
clinical
applications
CTC.
Abstract
The
existence
of
many
background
blood
cells
hinders
the
accurate
identification
circulating
tumor
(CTCs)
in
cancer
patients.
To
unlock
this
limitation,
a
hydrodynamic
sorting‐mechanotyping
cytometry
(HSMC)
integrated
with
sorting‐concentration
chip
and
detection
is
proposed
for
simultaneously
achieving
high‐throughput
cell
sorting
multi‐parameter
mechanotyping
sorted
cells.
HSMC
adopts
spiral
inertial
microfluidics
label‐free
manner,
allowing
efficient
enrichment
from
large
Then,
are
concentrated
by
concentration
unit
finally
passed
through
deformation.
has
high
throughput
can
successfully
reveal
differences
cellular
mechanical
properties.
After
characterizing
optimizing
single
chips,
white
(WBCs)
three
types
(A549,
MCF‐7,
MDA‐MB‐231
cells)
achieved.
accuracies
WBCs
different
all
larger
than
94%,
while
highest
accuracy
up
to
99.2%.
This
study
envisions
that
will
offer
an
avenue
analysis
intrinsic
mechanics
clinical
medicine.
The Analyst,
Год журнала:
2023,
Номер
148(12), С. 2758 - 2766
Опубликована: Янв. 1, 2023
This
paper
introduces
an
enclosed
microfluidic
chip
that
integrates
sample
preparation
and
the
chamber-based
digital
polymerase
chain
reaction
(cdPCR).
The
of
includes
nucleic
acid
extraction
purification
based
on
magnetic
beads,
which
adsorb
acids
by
moving
around
chambers
to
complete
reactions
including
lysis,
washing,
elution.
cdPCR
area
consists
tens
thousands
regularly
arranged
microchambers.
After
processes
are
completed,
purified
can
be
directly
introduced
into
microchambers
for
amplification
detection
chip.
performance
quantification
system
were
examined
using
synthetic
SARS-CoV-2
plasmid
templates
at
concentrations
ranging
from
101-105
copies
per
μL.
Further
on,
a
simulated
clinical
was
used
test
system,
integrated
able
accurately
detect
virus
particle
samples
doped
with
interference
(saliva)
limit
10
could
provide
promising
tool
point-of-care
testing
pathogenic
infections.
Electrophoresis,
Год журнала:
2023,
Номер
44(9-10), С. 775 - 783
Опубликована: Март 9, 2023
Conventional
cancer
diagnosis
needs
to
excise
diseased
tissue
from
the
patient's
body
for
biopsy,
causing
severe
injury
patients.
Liquid
biopsy
(LB),
with
superior
advantage
of
minimal
invasiveness,
has
shown
its
ability
in
real-time
and
been
developing
promising
diagnostic
instruments.
However,
until
today,
developed
instrument
still
cannot
be
an
alternative
majority
research
clinical
settings.
In
this
paper,
we
first
summarize
challenges
limitations
suffered
by
existing
LB
instrument.
Then,
opportunities
future
progression
next-generation
are
discussed
detail.
all,
hope
that
can
eventually
integrated
into
workflow
serve
as
a
validated
reliable
tool
diagnosis.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 16, 2025
The
existence
of
massive
amounts
red
blood
cells
(RBCs)
and
other
cell
components
poses
a
challenge
for
the
efficient
extraction
white
(WBCs)
from
blood.
Herein,
we
proposed
modular
inertial
microfluidics
WBCs
lysed
samples.
different
WBC
needs
could
be
met
by
flexibly
combining
washing
module
concentration
in
microfluidics.
First,
respective
optimal
flow
rates
modules
with
channel
sizes
were
explored.
Then,
based
on
combination
modules,
three
modes
to
meet
processing
large-volume
sample,
low
volume
expansion,
easy
operation.
replacement
efficiency
recovery
rate
target
particles
all
greater
than
93%.
Finally,
samples
was
tested.
combined
use
Modes
1
2
achieved
93.1%
93.6%,
while
single
Mode
3
97.1%
95.9%.
Compared
traditional
centrifuges,
our
showed
better
performance
reduced
residual
RBC
fragments.
More
importantly,
it
provided
an
excellent
free
capability
needs.