Path of Science,
Год журнала:
2023,
Номер
9(8), С. 7001 - 7014
Опубликована: Авг. 31, 2023
Lab-on-chip
(LoC)
platforms
are
disruptive
technologies
in
analytical
chemistry
and
bioengineering
known
to
enable
miniaturised
sensitive
analysis
of
complex
biological
chemical
samples.
Electrochemical
detection
became
one
the
most
explored
among
several
readout
methods,
hence
its
versatility,
robustness,
straightforward
execution,
sensitivity,
portability.
Therefore,
this
brief
review
critically
examines
principles,
applications,
prospects
electrochemical
LoC
technologies,
highlighting
significance
advancing
various
fields,
including
clinical
diagnostics,
environmental
monitoring,
drug
discovery.
Angewandte Chemie International Edition,
Год журнала:
2024,
Номер
63(20)
Опубликована: Март 9, 2024
Abstract
High‐precision
viral
detection
at
point
of
need
with
clinical
samples
plays
a
pivotal
role
in
the
diagnosis
infectious
diseases
and
control
global
pandemic.
However,
complexity
that
often
contain
very
low
concentrations
makes
it
huge
challenge
to
develop
simple
diagnostic
devices
do
not
require
any
sample
processing
yet
are
capable
meeting
performance
metrics
such
as
high
sensitivity
specificity.
Herein
we
describe
new
single‐pot
single‐step
electrochemical
method
uses
real‐time
kinetic
profiling
interaction
between
high‐affinity
aptamer
an
antigen
on
surface.
This
generates
many
data
points
per
sample,
which
when
combined
machine
learning,
can
deliver
highly
accurate
test
results
short
testing
time.
We
demonstrate
this
concept
using
both
SARS‐CoV‐2
Influenza
A
viruses
model
specifically
engineered
aptamers.
Utilizing
technique
diagnose
COVID‐19
37
real
human
saliva
specificity
100
%
(27
true
negatives
10
positives,
0
false
negative
positive),
showcases
superb
precision
method.
ACS Sensors,
Год журнала:
2023,
Номер
8(9), С. 3276 - 3293
Опубликована: Авг. 3, 2023
Electrochemical
affinity
biosensors
are
evolving
at
breakneck
speed,
strengthening
and
colonizing
more
niches
drawing
unimaginable
roadmaps
that
increasingly
make
them
protagonists
of
our
daily
lives.
They
achieve
this
by
combining
their
intrinsic
attributes
with
those
acquired
leveraging
the
significant
advances
occurred
in
(nano)materials
technology,
bio(nano)materials
nature-inspired
receptors,
gene
editing
amplification
technologies,
signal
detection
processing
techniques.
The
aim
Perspective
is
to
provide,
support
recent
representative
illustrative
literature,
an
updated
critical
view
repertoire
opportunities,
innovations,
applications
offered
electrochemical
fueled
key
alliances
indicated.
In
addition,
imminent
challenges
these
biodevices
must
face
new
directions
which
they
envisioned
as
players
discussed.
Analytical and Bioanalytical Chemistry,
Год журнала:
2023,
Номер
416(9), С. 2151 - 2172
Опубликована: Июль 7, 2023
Abstract
In
the
era
that
we
seek
personalization
in
material
things,
it
is
becoming
increasingly
clear
individualized
management
of
medicine
and
nutrition
plays
a
key
role
life
expectancy
quality
life,
allowing
participation
to
some
extent
our
welfare
use
societal
resources
rationale
equitable
way.
The
implementation
precision
are
highly
complex
challenges
which
depend
on
development
new
technologies
able
meet
important
requirements
terms
cost,
simplicity,
versatility,
determine
both
individually
simultaneously,
almost
real
time
with
required
sensitivity
reliability,
molecular
markers
different
omics
levels
biofluids
extracted,
secreted
(either
naturally
or
stimulated),
circulating
body.
Relying
representative
pioneering
examples,
this
review
article
critically
discusses
recent
advances
driving
position
electrochemical
bioplatforms
as
one
winning
horses
for
suitable
tools
advanced
diagnostics,
therapy,
nutrition.
addition
critical
overview
state
art,
including
groundbreaking
applications
ahead,
concludes
personal
vision
imminent
roadmap.
Analytical and Bioanalytical Chemistry,
Год журнала:
2024,
Номер
416(30), С. 7225 - 7247
Опубликована: Апрель 19, 2024
Abstract
Electrochemical
biosensing
continues
to
advance
tirelessly,
overcoming
barriers
that
have
kept
it
from
leaving
research
laboratories
for
many
years.
Among
them,
its
compromised
performance
in
complex
biological
matrices
due
fouling
or
receptor
stability
issues,
the
limitations
determining
toxic
and
small
analytes,
use,
conditioned
commercial
availability
of
receptors
exploration
natural
molecular
interactions,
deserved
be
highlighted.
To
address
these
challenges,
addition
intrinsic
properties
electrochemical
biosensing,
coupling
with
biomimetic
materials
has
played
a
fundamental
role,
among
which
bioinspired
phage
peptide
probes
stand
out.
The
versatility
design
employment
opened
an
unimaginable
plethora
possibilities
improving
their
far
beyond
development
highly
sensitive
selective
devices.
state
art
offers
robust
electroanalytical
biotools,
capable
operating
samples
exciting
opportunities
discover
determine
targets
regardless
toxicity
size,
bioreceptors,
prior
knowledge
interactions.
With
all
this
mind,
review
panoramic,
novel,
updated
vision
both
tremendous
advances
offered
by
combination
biosensors
challenges
efforts
are
envisioned
immediate
future.
Graphical
ACS Sensors,
Год журнала:
2023,
Номер
8(9), С. 3380 - 3388
Опубликована: Сен. 6, 2023
This
paper
presents
a
dual-aptamer
scheme
to
mitigate
signal
drifts
caused
by
structure-switching
aptamers
during
long-term
monitoring.
Electrochemical
aptamer-based
(E-AB)
biosensors
have
recently
shown
great
potential
for
continuous
in
vivo
However,
the
accuracy
of
detection
is
often
limited
signaling
drifts.
Traditional
approaches
rely
on
kinetic
differential
measurements
(KDM)
coupled
with
square-wave
voltammetry
eliminate
these
Yet,
we
discovered
that
KDM
does
not
apply
universally
all
aptamers,
as
their
responses
at
different
SWV
frequencies
heavily
characteristics
and
electron
transfer
(ET)
kinetics
redox
reporters.
In
light
this,
propose
"dual-aptamer"
utilizes
two
each
responding
differently
same
target
molecule
cancel
out
drift.
These
paired
are
identified
through
(1)
screening
from
an
existing
pool
(2)
engineering
behavior
We
demonstrate
aptamer
pair
presence
ampicillin
ATP
molecules
show
exhibits
similar
undiluted
goat
serum.
By
implementing
drift
cancelation,
sensor
reduced
factor
370.
Additionally,
enables
increased
recording
throughput
leveraging
readout
electronics.
The
authors
believe
proposed
technique
holds
significant
benefits
Micromachines,
Год журнала:
2023,
Номер
14(12), С. 2187 - 2187
Опубликована: Ноя. 30, 2023
Microfluidic
technology
has
revolutionized
device
fabrication
by
merging
principles
of
fluid
dynamics
with
technologies
from
chemistry,
physics,
biology,
material
science,
and
microelectronics.
systems
manipulate
small
volumes
fluids
to
perform
automated
tasks
applications
ranging
chemical
syntheses
biomedical
diagnostics.
The
advent
low-cost
3D
printers
the
development
microfluidic
systems.
For
measuring
molecules,
printing
offers
cost-effective,
time,
ease-of-designing
benefits.
In
this
paper,
we
present
a
comprehensive
tutorial
for
design,
optimization,
validation
creating
3D-printed
immunoarray
ultrasensitive
detection
multiple
protein
biomarkers.
target
is
point
care
array
determine
five
biomarkers
aggressive
cancers.
design
phase
involves
defining
dimensions
microchannels,
reagent
chambers,
wells,
optimizing
parameters
methods.
study,
physical
underwent
iterations
optimize
key
features,
such
as
developing
open
wells
uniform
signal
distribution
flap
covering
during
assay.
Then,
full
optimization
sensitivity
limit
(LOD)
was
performed,
calibration
plots
were
generated
assess
linear
dynamic
ranges
LODs.
Varying
characteristics
among
highlighted
need
tailored
assay
conditions.
Spike-recovery
studies
confirmed
assay's
accuracy.
Overall,
paper
showcases
methodology,
rigor,
innovation
involved
in
designing
immunoarray.
Optimized
parameters,
equations,
accuracy
data
contribute
valuable
metrics
future
biomarker
analyses.
