Passive microfluidic devices for cell separation

Biotechnology Advances, Год журнала: 2024, Номер 71, С. 108317 - 108317

Опубликована: Янв. 13, 2024

Язык: Английский

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Proteomics-on-a-Chip – Microfluidics Meets Proteomics

Biosensors and Bioelectronics, Год журнала: 2025, Номер 273, С. 117122 - 117122

Опубликована: Янв. 9, 2025

Язык: Английский

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Recent Developments in Inertial and Centrifugal Microfluidic Systems along with the Involved Forces for Cancer Cell Separation: A Review

Sensors, Год журнала: 2023, Номер 23(11), С. 5300 - 5300

Опубликована: Июнь 2, 2023

The treatment of cancers is a significant challenge in the healthcare context today. Spreading circulating tumor cells (CTCs) throughout body will eventually lead to cancer metastasis and produce new tumors near healthy tissues. Therefore, separating these invading extracting cues from them extremely important for determining rate progression inside development individualized treatments, especially at beginning process. continuous fast separation CTCs has recently been achieved using numerous techniques, some which involve multiple high-level operational protocols. Although simple blood test can detect presence circulation system, detection still restricted due scarcity heterogeneity CTCs. more reliable effective techniques thus highly desired. technology microfluidic devices promising among many other bio-chemical bio-physical technologies. This paper reviews recent developments two types devices, are based on size and/or density cells, cells. goal this review identify knowledge or gaps suggest future works.

Язык: Английский

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High-throughput and simultaneous inertial separation of tumor cells and clusters from malignant effusions using spiral-contraction-expansion channels

Microsystems & Nanoengineering, Год журнала: 2024, Номер 10(1)

Опубликована: Март 12, 2024

Abstract Tumor cell clusters are regarded as critical factors in cancer pathophysiology, and increasing evidence of their higher treatment resistance metastasis compared to single tumor cells has been obtained. However, existing separation methods that designed for cannot be used simultaneously purify clusters. To address this problem, we demonstrated a microfluidic approach the high-throughput, continuous-flow ternary cells, clusters, WBCs from clinical pleural or abdominal effusions by coupling slanted spiral channels periodic contraction-expansion arrays. We first systematically explored influence particle size flow rate on focusing. The performance indicated 94.0% were removed more than 97% MDA-MB-231 recovered at high 3500 µL/min. Moreover, 90% effectively preserved after separation. Finally, successfully applied our device different malignant collected patients with metastatic cancer. Thus, spiral-contraction-expansion potential sample pretreatment tool cytological diagnosis effusions.

Язык: Английский

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Cascaded elasto-inertial separation of malignant tumor cells from untreated malignant pleural and peritoneal effusions

Lab on a Chip, Год журнала: 2024, Номер 24(4), С. 697 - 706

Опубликована: Янв. 1, 2024

A cascaded elasto-inertial cell separation device for pretreatment-free, high-recovery-ratio, and high-purity of malignant tumor cells from clinical pleural peritoneal effusions.

Язык: Английский

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Integrated Microfluidics for Single‐Cell Separation and On‐Chip Analysis: Novel Applications and Recent Advances

Small Science, Год журнала: 2024, Номер 4(4)

Опубликована: Фев. 2, 2024

From deciphering infection and disease mechanisms to identifying novel biomarkers personalizing treatments, the characteristics of individual cells can provide significant insights into a variety biological processes facilitate decision‐making in biomedical environments. Conventional single‐cell analysis methods are limited terms cost, contamination risks, sample volumes, times, throughput, sensitivity, selectivity. Although microfluidic approaches have been suggested as low‐cost, information‐rich, high‐throughput alternative conventional isolation methods, limitations such necessary off‐chip pre‐ post‐processing well systems designed for workflows restricted their applications. In this review, comprehensive overview recent advances integrated microfluidics on‐chip three prominent application domains provided: investigation somatic (particularly cancer immune cells), stem cells, microorganisms. Also, use cell separation (e.g., dielectrophoresis) unconventional or ways, which advance integration multiple systems, is discussed. Finally, critical discussion related current how they could be overcome provided.

Язык: Английский

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Synergistic effects of dielectrophoretic and magnetophoretic forces on continuous cell separation via pinched flow fractionation

Physics of Fluids, Год журнала: 2025, Номер 37(2)

Опубликована: Фев. 1, 2025

The challenges of constraints and imprecision in chemical analysis medical diagnosis are particularly evident the separation blood cells. Pinched flow fractionation, a passive microfluidic technique, has gained attention for its potential size-based cell separation. Enhancing efficiency is crucial, especially through integration with external forces or active methods. This study explores use dielectrophoresis (DEP) magnetophoresis (MP) to separate platelet cells, red white circulating tumor These were analyzed both independently simultaneously. To achieve optimal separation, Navier–Stokes equations, Newton's second law, system's electric magnetic fields modeled. Experiments conducted branched microchannel assess impact various parameters, including buffer rate, pinched section width, applied voltage, field frequency, intensity. revealed approximately 99% under conditions Wp=20 μm, Q2=1250 μl/h, f=100 kHz, V=3 V, M=1.5 T. findings demonstrate that while DEP MP individually enhance their simultaneous application significantly improves precision, achieving complete optimized system holds great promise applications analytical chemistry diagnostics, analysis.

Язык: Английский

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Design and Development of a Traveling Wave Ferro-Microfluidic Device and System Rig for Potential Magnetophoretic Cell Separation and Sorting in a Water-Based Ferrofluid

Micromachines, Год журнала: 2023, Номер 14(4), С. 889 - 889

Опубликована: Апрель 21, 2023

The timely detection and diagnosis of diseases accurate monitoring specific genetic conditions require rapid separation, sorting, direction target cell types toward a sensor device surface. In that regard, cellular manipulation, sorting are progressively finding application potential within various bioassay applications such as medical disease diagnosis, pathogen detection, testing. aim this paper is to present the design development simple traveling wave ferro-microfluidic system rig purposed for manipulation magnetophoretic separation cells in water-based ferrofluids. This details full: (1) method tailoring cobalt ferrite nanoparticles diameter size ranges (10–20 nm), (2) potentially separating magnetic nanoparticles, (3) ferrofluid with non-magnetic microparticles, (4) producing electric field channel magnetizing manipulating nonmagnetic particles channel. results reported work demonstrate proof concept device. proof-of-concept study. model an improvement over existing excitation microfluidic designs heat efficiently removed from circuit board allow range input currents frequencies manipulate particles. Although did not analyze particles, (surrogates materials) entities can be separated and, some cases, continuously pushed through based on amperage, size, frequency, electrode spacing. establish developed may used effective platform microparticle sorting.

Язык: Английский

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Polydimethylsiloxane Surface Modification of Microfluidic Devices for Blood Plasma Separation

Polymers, Год журнала: 2024, Номер 16(10), С. 1416 - 1416

Опубликована: Май 16, 2024

Over the last decade, researchers have developed a variety of new analytical and clinical diagnostic devices. These devices are predominantly based on microfluidic technologies, where biological samples can be processed manipulated for collection detection important biomolecules. Polydimethylsiloxane (PDMS) is most commonly used material in fabrication these However, it has hydrophobic nature (contact angle with water 110°), leading to poor wetting behavior issues related mixing fluids, difficulties obtaining uniform coatings, reduced efficiency processes such as plasma separation molecule (protein adsorption). This work aimed consider aspects PDMS applications, surface modification methods. Therefore, we studied characterized two methods hydrophilic surfaces: by bulk mixture immersion method. To modify properties, three different surfactants were both (Pluronic

Язык: Английский

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An acoustofluidic device for the automated separation of platelet-reduced plasma from whole blood

Microsystems & Nanoengineering, Год журнала: 2024, Номер 10(1)

Опубликована: Июнь 24, 2024

Abstract Separating plasma from whole blood is an important sample processing technique required for fundamental biomedical research, medical diagnostics, and therapeutic applications. Traditional protocols isolation require multiple centrifugation steps or multiunit microfluidic to sequentially remove large red cells (RBCs) white (WBCs), followed by the removal of small platelets. Here, we present acoustofluidic platform capable efficiently removing RBCs, WBCs, platelets in a single step. By leveraging differences acoustic impedances fluids, our device generates significantly greater forces on suspended particles than conventional approaches, enabling both smaller unit. As result, undiluted human can be processed (>90%) at low voltages (25 Vpp). The ability successfully without altering properties proteins antibodies creates numerous potential applications as well plasma-based diagnostics therapeutics. Furthermore, nature offers advantages such portability, cost efficiency, process small-volume samples.

Язык: Английский

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