Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Chemical Reviews, Год журнала: 2024, Номер 124(15), С. 8825 - 9014

Опубликована: Июль 25, 2024

Luminescence imaging is a powerful and versatile technique for investigating cell physiology pathology in living systems, making significant contributions to life science research clinical diagnosis. In recent years, luminescent transition metal complexes have gained attention diagnostic therapeutic applications due their unique photophysical photochemical properties. this Review, we provide comprehensive overview of the development bioimaging biosensing applications, with focus on centers d

Язык: Английский

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Molecularly Engineered Room-Temperature Phosphorescence for Biomedical Application: From the Visible toward Second Near-Infrared Window

Chemical Reviews, Год журнала: 2023, Номер 123(24), С. 13966 - 14037

Опубликована: Ноя. 22, 2023

Phosphorescence, characterized by luminescent lifetimes significantly longer than that of biological autofluorescence under ambient environment, is great value for biomedical applications. Academic evidence fluorescence imaging indicates virtually all metrics (sensitivity, resolution, and penetration depths) are improved when progressing into wavelength regions, especially the recently reported second near-infrared (NIR-II, 1000–1700 nm) window. Although emission probes does matter, it not clear whether guideline "the wavelength, better effect" still suitable developing phosphorescent probes. For tissue-specific bioimaging, long-lived probes, even if they emit visible phosphorescence, enable accurate visualization large deep tissues. studies dealing with bioimaging tiny architectures or dynamic physiopathological activities, prerequisite rigorous planning long-wavelength being aware cooperative contribution long wavelengths improving spatiotemporal depth, sensitivity bioimaging. In this Review, emerging molecular engineering methods room-temperature phosphorescence discussed through lens photophysical mechanisms. We highlight roles from to NIR-II windows toward bioapplications. To appreciate such advances, challenges prospects in rapidly growing described.

Язык: Английский

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Photooxidation triggered ultralong afterglow in carbon nanodots

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Март 15, 2024

Abstract It remains a challenge to obtain biocompatible afterglow materials with long emission wavelengths, durable lifetimes, and good water solubility. Herein we develop photooxidation strategy construct near-infrared carbon nanodots an extra-long lifetime of up 5.9 h, comparable that the well-known rare-earth or organic long-persistent luminescent materials. Intriguingly, size-dependent evolution from 3.4 h has been observed systems in aqueous solution. With structural/ultrafast dynamics analysis density functional theory simulations, reveal persistent luminescence is activated by photooxidation-induced dioxetane intermediate, which can slowly release convert energy into luminous via steric hindrance effect nanoparticles. luminescence, tissue penetration depth 20 mm be achieved. Thanks high signal-to-background ratio, biological safety cancer-specific targeting ability nanodots, ultralong-afterglow guided surgery successfully performed on mice model remove tumor tissues accurately, demonstrating potential clinical applications. These results may facilitate development long-lasting for precision resection.

Язык: Английский

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Noninvasive Imaging of Tumor Glycolysis and Chemotherapeutic Resistance via De Novo Design of Molecular Afterglow Scaffold

Journal of the American Chemical Society, Год журнала: 2023, Номер 145(44), С. 24386 - 24400

Опубликована: Окт. 26, 2023

Chemotherapeutic resistance poses a significant challenge in cancer treatment, resulting the reduced efficacy of standard chemotherapeutic agents. Abnormal metabolism, particularly increased anaerobic glycolysis, has been identified as major contributing factor to resistance. To address this issue, noninvasive imaging techniques capable visualizing tumor glycolysis are crucial. However, currently available methods (such PET, MRI, and fluorescence) possess limitations terms sensitivity, safety, dynamic capability, autofluorescence. Here, we present de novo design unique afterglow molecular scaffold based on hemicyanine rhodamine dyes, which holds promise for low-background optical imaging. In contrast previous designs, exhibits responsive "OFF-ON" signals through spirocyclization, thus enabling simultaneous control photodynamic effects luminescence efficacy. This leads larger range, broader detection higher signal enhancement ratio, sensitivity. Furthermore, integration multiple functionalities simplifies probe design, eliminates need spectral overlap, enhances reliability. Moreover, have expanded applications by developing various probes different targets. Notably, developed water-soluble pH-responsive nanoprobe living mice. monitors glycolytic inhibitors or oxidative phosphorylation providing valuable tool evaluating cell sensitivity these inhibitors. Therefore, new presents promising approach understanding monitoring resistance, guiding precision medicine future.

Язык: Английский

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Remote Control of Energy Transformation‐Based Cancer Imaging and Therapy

Advanced Materials, Год журнала: 2024, Номер 36(27)

Опубликована: Март 29, 2024

Cancer treatment requires precise tumor-specific targeting at specific sites that allows for high-resolution diagnostic imaging and long-term patient-tailorable cancer therapy; while, minimizing side effects largely arising from non-targetability. This can be realized by harnessing exogenous remote stimuli, such as tissue-penetrative ultrasound, magnetic field, light, radiation, enable local activation therapy in deep tumors. A myriad of nanomedicines efficiently activated when the energy stimuli transformed into another type energy. review discusses control transformation targetable, efficient, therapy. Such ultrasonic, magnetic, photonic, radiative, radioactive mechanical, thermal, chemical, radiative to a variety modalities. The current article describes multimodal where serial cascade or multiple types occur. includes not only hyperthermia, radiation but also emerging thermoelectric, pyroelectric, piezoelectric therapies treatment. It illustrates resonance, fluorescence, computed tomography, photoluminescence, photoacoustic imaging-guided therapies. highlights afterglow eliminate autofluorescence sustained signal emission after excitation.

Язык: Английский

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Edible Long‐Afterglow Photoluminescent Materials for Bioimaging

Advanced Materials, Год журнала: 2024, Номер 36(30)

Опубликована: Май 13, 2024

Abstract Confining luminophores into modified hydrophilic matrices or polymers is a straightforward and widely used approach for afterglow bioimaging. However, the quantum yield lifetime of related material remain unsatisfactory, severely limiting using effect especially deep‐tissue time‐resolved imaging. This fact largely stems from dilemma between biocompatibility quenching water environment. Herein an in situ metathesis promoted doping strategy presented, namely, mixing ≈10 −3 weight ratio organic‐emitter multicarboxylates with inorganic salt reactants, followed by reactions to prepare series but water‐insoluble organic–inorganic materials. leads formation edible long‐afterglow photoluminescent materials superior excellent bioimaging effect. The phosphorescence can reach dozens percent (the highest case: 66.24%), together lasting coupes seconds. Specifically, barium meal formed coronene emitter BaSO 4 matrix applied animal experiments gavage, bright stomach imaging observed instruments mobile phone after ceasing photoexcitation deep tissue penetration. allows flexible dosage during bioimaging, facilitating development real‐time probing theranostic technology.

Язык: Английский

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2, 1, 3‐Benzothiadiazole Derivative Small Molecule Fluorophores for NIR‐II Bioimaging

Advanced Functional Materials, Год журнала: 2024, Номер 34(16)

Опубликована: Янв. 4, 2024

Abstract Second near‐infrared (NIR‐II) bioimaging technology shows broad application prospects in the field of biomedical due to its significant superiority deep tissue penetration and high signal‐to‐background ratio. Among them, organic small molecule fluorophores have received progressively more consideration their low biotoxicity, biocompatibility, rapid clearance, good modification properties. As key acceptor core, 2, 1, 3‐benzothiadiazole (BTD) derivative plays an essential role construction donor‐acceptor‐donor type NIR‐II fluorophores. This mini‐review summarizes research development based on BTD derivatives bioimaging, focusing molecular design photophysical properties, introduces a part applications biological field. Furthermore, challenges BTD‐based future are discussed .

Язык: Английский

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Integration of Motion and Stillness: A Paradigm Shift in Constructing Nearly Planar NIR-II AIEgen with Ultrahigh Molar Absorptivity and Photothermal Effect for Multimodal Phototheranostics

Journal of the American Chemical Society, Год журнала: 2025, Номер unknown

Опубликована: Янв. 15, 2025

The two contradictory entities in nature often follow the principle of unity opposites, leading to optimal overall performance. Particularly, aggregation-induced emission luminogens (AIEgens) with donor–acceptor (D–A) structures exhibit tunable optical properties and versatile functionalities, offering significant potential revolutionize cancer treatment. However, trapped by low molar absorptivity (ε) owing distorted configurations, ceilings their photon-harvesting capability corresponding phototheranostic performance still fall short. Therefore, a research paradigm from twisted configuration near-planar structure featuring high ε is urgently needed for AIEgens development. Herein, introducing strategy "motion stillness" into highly planar A–D–A skeleton, we successfully developed near-infrared (NIR)-II AIEgen Y5-2BO-2BTF, which boasts an impressive 1.06 × 105 M–1 cm–1 photothermal conversion efficiency (PCE) 77.8%. modification steric hindrance on benzene ring acceptor unit aggregation-caused quenching counterpart Y5-2BO, meta-CF3-substituted naphthyl, leads reversely staggered packing various intermolecular noncovalent conformational locks Y5-2BO-2BTF ("stillness"). Furthermore, −CF3 moiety acted as flexible motion ultralow energy barrier, significantly facilitating process loose aggregates ("motion"). Accordingly, nanoparticles enabled tumor eradication pulmonary metastasis inhibition through NIR-II fluorescence-photoacoustic-photothermal imaging-navigated type I photodynamic-photothermal therapy. This work provides first evidence that conformation stacking arrangement could serve novel molecular design direction AIEgens, shedding new light constructing superior agents bioimaging

Язык: Английский

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A Highly Bright Near‐Infrared Afterglow Luminophore for Activatable Ultrasensitive In Vivo Imaging

Angewandte Chemie International Edition, Год журнала: 2023, Номер 63(4)

Опубликована: Ноя. 29, 2023

Afterglow luminescence imaging probes, with long-lived emission after cessation of light excitation, have drawn increasing attention in biomedical field owing to their elimination autofluorescence. However, current afterglow agents always suffer from an unsatisfactory signal intensity and complex systems consisting multiple ingredients. To address these issues, this study reports a near-infrared (NIR) luminophore (TPP-DO) by chemical conjugation substrate photosensitizer acting as both initiator energy relay unit into single molecule, resulting intramolecular transfer process improve the brightness. The constructed TPP-DO NPs emit strong NIR up 10

Язык: Английский

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Superoxide Anion-Mediated Afterglow Mechanism-Based Water-Soluble Zwitterion Dye Achieving Renal-Failure Mice Detection

Journal of the American Chemical Society, Год журнала: 2023, Номер 145(49), С. 26736 - 26746

Опубликована: Ноя. 28, 2023

Afterglow materials-based biological imaging has promising application prospects, due to negligible background. However, currently available afterglow materials mainly include inorganic as well some organic nanoparticles, which are difficult translate the clinic, resulting from non-negligible metabolic toxicity and even leakage risk of heavy metals. Although building small molecules could solve such obstacles, with ability extremely scarce, especially a sufficient renal capacity. To address these issues, herein, we designed water-soluble zwitterion Cy5-NF capacity luminescence, relied on an intramolecular cascade reaction between superoxide anion (O2•–, instead 1O2) release luminescence. Of note, compared different reference contrast agents, not only had excellent properties but also rapid metabolism rate (half-life period, t1/2, around 10 min) good biocompatibility. Unlike prior nanosystems possessing large size, for first time, achieved construction showed higher sensitivity signal-to-background ratio in than fluorescence kidney. Moreover, longer kidney retention time renal-failure mice (t1/2 more 15 min). More importantly, can be metabolized very quickly severe 25–30 min), greatly improved biosecurity. Therefore, optimistic that O2•–-mediated mechanism-based is clinical application, detection failure.

Язык: Английский

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