Advanced design and applications of digital microfluidics in biomedical fields: An update of recent progress

Biosensors and Bioelectronics, Год журнала: 2023, Номер 242, С. 115723 - 115723

Опубликована: Окт. 1, 2023

Язык: Английский

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On-Site Bioaerosol Sampling and Airborne Microorganism Detection Technologies

Biosensors, Год журнала: 2024, Номер 14(3), С. 122 - 122

Опубликована: Фев. 24, 2024

Bioaerosols are small airborne particles composed of microbiological fragments, including bacteria, viruses, fungi, pollens, and/or by-products cells, which may be viable or non-viable wherever applicable. Exposure to these agents can cause a variety health issues, such as allergic and infectious diseases, neurological disorders, cancer. Therefore, detecting identifying bioaerosols is crucial, bioaerosol sampling key step in any investigation. This review provides an overview the current methods, both passive active, well their applications limitations for rapid on-site monitoring. The challenges trends microorganisms using molecular immunological methods also discussed, along with summary outlook development prompt monitoring technologies.

Язык: Английский

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Integrating single-cell and spatially resolved transcriptomic strategies to survey the astrocyte response to stroke in male mice

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Фев. 21, 2024

Abstract Astrocytes, a type of glial cell in the central nervous system (CNS), adopt diverse states response to injury that are influenced by their location relative insult. Here, we describe platform for spatially resolved, single-cell transcriptomics and proteomics, called tDISCO ( tissue- digital microfluidic isolation single cells -Omics). We use alongside two high-throughput platforms spatial (Visium) (10X Chromium) examine heterogeneity astrocyte cortical ischemic stroke male mice. show integration Visium 10X Chromium datasets infers populations, proximal or distal site, while determines boundaries molecular profiles define these populations. find astrocytes differences lipid shuttling, with enriched expression Apoe Fabp5 . Our provide resource understanding roles showcase utility hypothesis-driven, resolved experiments.

Язык: Английский

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AM-DMF-SCP: Integrated Single-Cell Proteomics Analysis on an Active Matrix Digital Microfluidic Chip

JACS Au, Год журнала: 2024, Номер 4(5), С. 1811 - 1823

Опубликована: Март 26, 2024

Single-cell proteomics offers unparalleled insights into cellular diversity and molecular mechanisms, enabling a deeper understanding of complex biological processes at the individual cell level. Here, we develop an integrated sample processing on active-matrix digital microfluidic chip for single-cell (AM-DMF-SCP). Employing AM-DMF-SCP approach data-independent acquisition (DIA), identify average 2258 protein groups in single HeLa cells within 15 min liquid chromatography gradient. We performed comparative analyses three tumor lines: HeLa, A549, HepG2, machine learning was utilized to unique features these lines. Applying characterize proteomes third-generation EGFR inhibitor, ASK120067-resistant (67R) their parental NCI-H1975 cells, observed potential correlation between elevated VIM expression 67R resistance, which is consistent with findings from bulk analyses. These results suggest that automated, robust, sensitive platform demonstrate providing valuable mechanisms.

Язык: Английский

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Open and closed microfluidics for biosensing

Materials Today Bio, Год журнала: 2024, Номер 26, С. 101048 - 101048

Опубликована: Апрель 4, 2024

Biosensing is vital for many areas like disease diagnosis, infectious prevention, and point-of-care monitoring. Microfluidics has been evidenced to be a powerful tool biosensing via integrating biological detection processes into palm-size chip. Based on the chip structure, microfluidics two subdivision types: open closed microfluidics, whose operation methods would diverse. In this review, we summarize fundamentals, liquid control methods, applications of separately, point out bottlenecks, propose potential directions microfluidics-based biosensing.

Язык: Английский

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A review of electrochemical sensing in droplet systems: droplet and digital microfluidics

Analytica Chimica Acta, Год журнала: 2025, Номер 1347, С. 343744 - 343744

Опубликована: Фев. 4, 2025

Язык: Английский

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Unlocking the potential of microfluidics in mass spectrometry-based immunopeptidomics for tumor antigen discovery

Cell Reports Methods, Год журнала: 2023, Номер 3(6), С. 100511 - 100511

Опубликована: Июнь 1, 2023

The identification of tumor-specific antigens (TSAs) is critical for developing effective cancer immunotherapies. Mass spectrometry (MS)-based immunopeptidomics has emerged as a powerful tool identifying TSAs physical molecules. However, current platforms face challenges in measuring low-abundance precise, sensitive, and reproducible manner from small needle-tissue biopsies (<1 mg). Inspired by recent advances single-cell proteomics, microfluidics technology offers promising solution to these limitations providing improved isolation human leukocyte antigen (HLA)-associated peptides with higher sensitivity. In this context, we highlight the sample preparation rationale immunopeptidomics. Additionally, provide an overview microfluidic methods, including microchip pillar arrays, valved-based systems, droplet microfluidics, digital discuss latest research on their application MS-based proteomics.

Язык: Английский

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Digital Microfluidics and Magnetic Bead‐Based Intact Proteoform Elution for Quantitative Top‐down Nanoproteomics of Single C. elegans Nematodes

Angewandte Chemie International Edition, Год журнала: 2023, Номер 62(28)

Опубликована: Апрель 17, 2023

While most nanoproteomics approaches for the analysis of low-input samples are based on bottom-up proteomics workflows, top-down enabling proteoform characterization still underrepresented. Using mammalian cell proteomes, we established a facile one-pot sample preparation protocol protein aggregation magnetic beads and intact elution using 40 % formic acid. Performed digital microfluidics device, workflow enabled sensitive analyses single Caenorhabditis elegans nematodes, thereby increasing number identifications compared to in-tube by 46 %. Label-free quantification nematodes grown under different conditions allowed identify changes in abundance proteoforms not distinguishable proteomics. The presented will facilitate proteoform-directed limited availability.

Язык: Английский

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Droplet-Based Microfluidics with Mass Spectrometry for Microproteomics

Engineering, Год журнала: 2024, Номер unknown

Опубликована: Сен. 1, 2024

Язык: Английский

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A low-cost electrowetting on dielectric semi-continuous pump for application to microfluidic reconfigurable devices

Experimental Thermal and Fluid Science, Год журнала: 2024, Номер 155, С. 111183 - 111183

Опубликована: Март 12, 2024

Язык: Английский

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