Digital microfluidic chip with photopatterned reactive sites for direct biomolecules immobilization and magnetic beads-free immunoassay DOI

Qiudi Xu,

Liu Ke, Yu He

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 424, P. 136893 - 136893

Published: Nov. 4, 2024

Language: Английский

Advanced design and applications of digital microfluidics in biomedical fields: An update of recent progress DOI
Chengbin Yang,

Xiangyu Gan,

Yuping Zeng

et al.

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 242, P. 115723 - 115723

Published: Oct. 1, 2023

Language: Английский

Citations

36
On-Site Bioaerosol Sampling and Airborne Microorganism Detection Technologies DOI Creative Commons

Afagh Rastmanesh,

Jayanta Sarmah Boruah, Min-Seok Lee

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(3), P. 122 - 122

Published: Feb. 24, 2024

Bioaerosols are small airborne particles composed of microbiological fragments, including bacteria, viruses, fungi, pollens, and/or by-products cells, which may be viable or non-viable wherever applicable. Exposure to these agents can cause a variety health issues, such as allergic and infectious diseases, neurological disorders, cancer. Therefore, detecting identifying bioaerosols is crucial, bioaerosol sampling key step in any investigation. This review provides an overview the current methods, both passive active, well their applications limitations for rapid on-site monitoring. The challenges trends microorganisms using molecular immunological methods also discussed, along with summary outlook development prompt monitoring technologies.

Language: Английский

Citations

10
Integrating single-cell and spatially resolved transcriptomic strategies to survey the astrocyte response to stroke in male mice DOI Creative Commons
E. Scott,

Nickie Safarian,

Daniela Lozano Casasbuenas

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 21, 2024

Abstract Astrocytes, a type of glial cell in the central nervous system (CNS), adopt diverse states response to injury that are influenced by their location relative insult. Here, we describe platform for spatially resolved, single-cell transcriptomics and proteomics, called tDISCO ( tissue- digital microfluidic isolation single cells -Omics). We use alongside two high-throughput platforms spatial (Visium) (10X Chromium) examine heterogeneity astrocyte cortical ischemic stroke male mice. show integration Visium 10X Chromium datasets infers populations, proximal or distal site, while determines boundaries molecular profiles define these populations. find astrocytes differences lipid shuttling, with enriched expression Apoe Fabp5 . Our provide resource understanding roles showcase utility hypothesis-driven, resolved experiments.

Language: Английский

Citations

9
AM-DMF-SCP: Integrated Single-Cell Proteomics Analysis on an Active Matrix Digital Microfluidic Chip DOI Creative Commons
Zhicheng Yang, Kai Jin,

Yi‐Min Chen

et al.

JACS Au, Journal Year: 2024, Volume and Issue: 4(5), P. 1811 - 1823

Published: March 26, 2024

Single-cell proteomics offers unparalleled insights into cellular diversity and molecular mechanisms, enabling a deeper understanding of complex biological processes at the individual cell level. Here, we develop an integrated sample processing on active-matrix digital microfluidic chip for single-cell (AM-DMF-SCP). Employing AM-DMF-SCP approach data-independent acquisition (DIA), identify average 2258 protein groups in single HeLa cells within 15 min liquid chromatography gradient. We performed comparative analyses three tumor lines: HeLa, A549, HepG2, machine learning was utilized to unique features these lines. Applying characterize proteomes third-generation EGFR inhibitor, ASK120067-resistant (67R) their parental NCI-H1975 cells, observed potential correlation between elevated VIM expression 67R resistance, which is consistent with findings from bulk analyses. These results suggest that automated, robust, sensitive platform demonstrate providing valuable mechanisms.

Language: Английский

Citations

9
Open and closed microfluidics for biosensing DOI Creative Commons

Tianxin Ge,

Wenxu Hu,

Zilong Zhang

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 26, P. 101048 - 101048

Published: April 4, 2024

Biosensing is vital for many areas like disease diagnosis, infectious prevention, and point-of-care monitoring. Microfluidics has been evidenced to be a powerful tool biosensing via integrating biological detection processes into palm-size chip. Based on the chip structure, microfluidics two subdivision types: open closed microfluidics, whose operation methods would diverse. In this review, we summarize fundamentals, liquid control methods, applications of separately, point out bottlenecks, propose potential directions microfluidics-based biosensing.

Language: Английский

Citations

8
A review of electrochemical sensing in droplet systems: droplet and digital microfluidics DOI Creative Commons
Kieu Chan Ly, Abrarkhan M. Pathan, Darius G. Rackus

et al.

Analytica Chimica Acta, Journal Year: 2025, Volume and Issue: 1347, P. 343744 - 343744

Published: Feb. 4, 2025

Language: Английский

Citations

1
Unlocking the potential of microfluidics in mass spectrometry-based immunopeptidomics for tumor antigen discovery DOI Creative Commons

Charlotte Stutzmann,

Jiaxi Peng, Zhaoguan Wu

et al.

Cell Reports Methods, Journal Year: 2023, Volume and Issue: 3(6), P. 100511 - 100511

Published: June 1, 2023

The identification of tumor-specific antigens (TSAs) is critical for developing effective cancer immunotherapies. Mass spectrometry (MS)-based immunopeptidomics has emerged as a powerful tool identifying TSAs physical molecules. However, current platforms face challenges in measuring low-abundance precise, sensitive, and reproducible manner from small needle-tissue biopsies (<1 mg). Inspired by recent advances single-cell proteomics, microfluidics technology offers promising solution to these limitations providing improved isolation human leukocyte antigen (HLA)-associated peptides with higher sensitivity. In this context, we highlight the sample preparation rationale immunopeptidomics. Additionally, provide an overview microfluidic methods, including microchip pillar arrays, valved-based systems, droplet microfluidics, digital discuss latest research on their application MS-based proteomics.

Language: Английский

Citations

18
Digital Microfluidics and Magnetic Bead‐Based Intact Proteoform Elution for Quantitative Top‐down Nanoproteomics of Single C. elegans Nematodes DOI Creative Commons
Jan Leipert,

Philipp T. Kaulich,

Max K. Steinbach

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(28)

Published: April 17, 2023

While most nanoproteomics approaches for the analysis of low-input samples are based on bottom-up proteomics workflows, top-down enabling proteoform characterization still underrepresented. Using mammalian cell proteomes, we established a facile one-pot sample preparation protocol protein aggregation magnetic beads and intact elution using 40 % formic acid. Performed digital microfluidics device, workflow enabled sensitive analyses single Caenorhabditis elegans nematodes, thereby increasing number identifications compared to in-tube by 46 %. Label-free quantification nematodes grown under different conditions allowed identify changes in abundance proteoforms not distinguishable proteomics. The presented will facilitate proteoform-directed limited availability.

Language: Английский

Citations

16
Droplet-Based Microfluidics with Mass Spectrometry for Microproteomics DOI Creative Commons
Hang Li,

Yudan Ma,

Rongxin Fu

et al.

Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

Citations

4
A low-cost electrowetting on dielectric semi-continuous pump for application to microfluidic reconfigurable devices DOI
Behzad Parsi,

Josh Augenstein,

R. Daniel Maynes

et al.

Experimental Thermal and Fluid Science, Journal Year: 2024, Volume and Issue: 155, P. 111183 - 111183

Published: March 12, 2024

Language: Английский

Citations

3