Life,
Год журнала:
2025,
Номер
15(2), С. 209 - 209
Опубликована: Янв. 30, 2025
Human
Immunodeficiency
Virus
(HIV)
remains
a
major
public
health
challenge
globally.
Recent
innovations
in
diagnostic
technology
have
opened
new
pathways
for
early
detection,
ongoing
monitoring,
and
more
individualized
patient
care,
yet
significant
barriers
persist
translating
these
advancements
into
clinical
settings.
This
review
highlights
the
cutting-edge
methods
emerging
from
basic
science
research,
including
molecular
assays,
biosensors,
next-generation
sequencing,
discusses
practical
logistical
challenges
involved
their
implementation.
By
analyzing
current
trends
techniques
management
strategies,
we
identify
critical
gaps
propose
integrative
approaches
to
bridge
divide
between
laboratory
innovation
effective
application.
work
emphasizes
need
comprehensive
education,
supportive
infrastructure,
multi-disciplinary
collaborations
enhance
utility
of
improving
outcomes
patients
with
HIV.
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Фев. 13, 2025
Accurate
detection
and
quantification
of
biomarkers
at
ultra-low
levels
is
critical
for
disease
diagnosis
effective
treatment.
Traditional
technologies
often
lack
the
sensitivity,
specificity,
throughput,
or
multiplexing
capacity
required
comprehensive
diagnostics,
providing
only
a
subset
these
requirements.
Here,
we
introduce
AVAC,
an
automated
optical
technology
rapid
accurate
biomarker
with
ultra-high
sensitivity
that
significantly
outperforms
standard
clinical
assays.
The
core
this
digital
counting
plasmonic
nanoparticles
used
as
labels,
enabling
multiplexed,
high-throughput
biomarkers.
Validation
studies
demonstrate
AVAC's
high
accuracy,
98.2%
specificity
limits
low
26
fg/mL
HIV
p24
protein
range
160
to
850
pg/mL
interleukin-6
(IL-6).
supports
multiplexed
assays
without
compromising
demonstrated
by
simultaneous
three
key
associated
cardiovascular
disease.
A
spanning
more
than
four
orders
magnitude
ensures
robust
from
concentrations,
its
ability
analyze
up
1,000
samples
per
hour
provides
throughput
suitable
large
laboratories.
With
unique
combination
capabilities,
versatile
platform
has
significant
potential
advance
biomarker-based
diagnostics
in
research
settings.
Microchimica Acta,
Год журнала:
2025,
Номер
192(3)
Опубликована: Фев. 12, 2025
Abstract
The
application
is
demonstrated
of
injection-molded
centrifugal
microfluidic
chips
with
integrated
optical
pH
sensors
for
multiplexed
detection
respiratory
syndrome
coronavirus
2
(SARS-CoV-2),
influenza
A,
and
B
RNA.
generated
sensitive
fluorescent
readouts
from
diagnostic
reverse
transcription
loop-mediated
isothermal
amplification
(RT-LAMP)
reactions;
limits
influenzas
A
B,
SARS-CoV-2
89,
245,
38
RNA
copies
per
reaction,
respectively,
were
attained.
Results
obtainable
within
44
min
48
B.
We
implemented
a
data
processing
strategy
based
on
numerical
derivatives
the
fluorescence
curves
that
allowed
reliable,
quantitative
thresholds
deciding
reaction
outcomes
enabled
100%
specificity.
This
work
demonstrates
utility
microfluidics
infectious
disease
diagnostics
point-of-care
applications.
Graphical
Micromachines,
Год журнала:
2025,
Номер
16(3), С. 243 - 243
Опубликована: Фев. 20, 2025
Polymerase
chain
reaction
(PCR)
chips
are
advanced,
microfluidic
platforms
that
have
revolutionized
biomarker
discovery
and
validation
because
of
their
high
sensitivity,
specificity,
throughput
levels.
These
miniaturize
traditional
PCR
processes
for
the
speed
precision
nucleic
acid
detection
relevant
to
advancing
drug
development.
Biomarkers,
which
useful
in
helping
explain
disease
mechanisms,
patient
stratification,
therapeutic
monitoring,
hard
identify
validate
due
complexity
biological
systems
limitations
techniques.
The
challenges
respond
include
high-throughput
capabilities
coupled
with
real-time
quantitative
analysis,
enabling
researchers
novel
biomarkers
greater
accuracy
reproducibility.
More
recent
design
improvements
further
expanded
functionality
also
digital
multiplex
technologies.
Digital
ideal
quantifying
rare
biomarkers,
is
essential
oncology
infectious
research.
In
contrast,
enable
simultaneous
analysis
multiple
targets,
therefore
simplifying
validation.
Furthermore,
single-cell
made
it
possible
detect
at
unprecedented
resolution,
hence
revealing
heterogeneity
within
cell
populations.
transforming
development,
target
identification,
efficacy
assessment.
They
play
a
major
role
development
companion
diagnostics
and,
therefore,
pave
way
personalized
medicine,
ensuring
right
receives
treatment.
While
this
tremendously
promising
technology
has
exhibited
many
regarding
its
scalability,
integration
other
omics
technologies,
conformity
regulatory
requirements,
still
prevail.
Future
breakthroughs
chip
manufacturing,
artificial
intelligence,
multi-omics
applications
will
expand
capabilities.
not
only
be
important
acceleration
but
raising
bar
improving
outcomes
hence,
global
health
care
as
these
technologies
continue
mature.
