Development of MWCNT/Gd2O3/SnO2 composite fabricated GCE for voltammetric detection of L- cysteine DOI Creative Commons
Arka Roy, Sudip Das, Arghya Chakravorty

et al.

Results in Surfaces and Interfaces, Journal Year: 2024, Volume and Issue: 16, P. 100267 - 100267

Published: Aug. 1, 2024

A highly sensitive electrochemical method for the determination of L- Cysteine (L-cys) was developed using a glassy carbon electrode (GCE) modified with composite bimetallic gadolinium oxide (Gd2O3) and stannous (SnO2) embedded on multi-walled nanotubes (MWCNT). This shows excellent electrocatalytic activity toward oxidation L-cys in phosphate buffer solution (0.1 M, pH 7). The proposed showed linear range 0.011 μM–27.7 μM, sensitivity 0.048 μA μM−1 cm−2 detection limit (LOD) 5.993 nM. X-Ray diffraction analysis (XRD), Field Emission Scanning Electron Microscopy (FE-SEM), Energy-Dispersive X-ray Analysis (EDAX) performed to characterize all synthesized materials prior fabrication. Linear sweep voltammetry (LSV) account rise anodic peak potential concluding current conductivity. Differential pulse (DPV) also confirm results. work introduces simple easy approach L-cys. Electrochemical impedance spectroscopy (EIS) Cyclic (CV) employed fabricated used investigate redox properties this at various scan rates. low-cost nanomaterial has wide stability reproducibility. practical applicability sensor confirmed by sensing real samples, possessing satisfactory recoveries (95.8 % 107.1 %).

Language: Английский

Design and Analysis of a Plasmonic Metasurface-Based Graphene Sensor for Highly Sensitive and Label-Free Detection of COVID-19 Biomarkers DOI
P. Nagarajan, Jacob Wekalao,

Natarajan Ashokkumar

et al.

Plasmonics, Journal Year: 2024, Volume and Issue: unknown

Published: July 27, 2024

Language: Английский

Citations

22
Plasmonic Biosensors for Health Monitoring: Inflammation Biomarker Detection DOI
Muhammad Amirul Islam, Jean‐François Masson

ACS Sensors, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

Surface plasmon resonance (SPR) and localized SPR (LSPR) biosensors have emerged as viable technologies in the clinical detection of biomarkers for a wide array health conditions. The success lies their ability to monitor real-time label-free complex biofluids. Recent breakthroughs nanotechnology surface chemistry significantly improved this feature, notably from incorporation advanced nanomaterials including gold nanoparticles, graphene, carbon nanotubes providing better sensor performance terms limits, stability, specificity. progress microfluidic integration has enabled detect multiple simultaneously biological samples. Taken together, these advances are closing gap use diagnostics point-of-care (POC) applications. While broadly applicable, latest advancements plasmonic biosensing overviewed using inflammation C-reactive protein (CRP), interleukins (ILs), tumor necrosis factor-α (TNF-α), procalcitonin (PCT), ferritin, fibrinogen series conditions, cardiovascular diseases, autoimmune disorders, infections, sepsis, key example We highlight developments design, nanomaterial integration, functionalization, multiplexing provide look forward applications by assessing current limitations exploring future directions translating monitoring. By enhancement diagnostic accuracy, reproducibility, accessibility, particularly POC settings, potential contribute personalized healthcare bring real-time, high-precision forefront practice.

Language: Английский

Citations

4
Gold nanoparticles-based biosensors: pioneering solutions for bacterial and viral pathogen detection—a comprehensive review DOI

Vishakha Suryakant Parkhe,

Arpita Tiwari

World Journal of Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 40(9)

Published: July 16, 2024

Language: Английский

Citations

10
Innovative Diagnostic Approaches and Challenges in the Management of HIV: Bridging Basic Science and Clinical Practice DOI Creative Commons

Mohd Afzal,

Shagun Agarwal, Rabab Hassan Elshaikh

et al.

Life, Journal Year: 2025, Volume and Issue: 15(2), P. 209 - 209

Published: Jan. 30, 2025

Human Immunodeficiency Virus (HIV) remains a major public health challenge globally. Recent innovations in diagnostic technology have opened new pathways for early detection, ongoing monitoring, and more individualized patient care, yet significant barriers persist translating these advancements into clinical settings. This review highlights the cutting-edge methods emerging from basic science research, including molecular assays, biosensors, next-generation sequencing, discusses practical logistical challenges involved their implementation. By analyzing current trends techniques management strategies, we identify critical gaps propose integrative approaches to bridge divide between laboratory innovation effective application. work emphasizes need comprehensive education, supportive infrastructure, multi-disciplinary collaborations enhance utility of improving outcomes patients with HIV.

Language: Английский

Citations

2
Bringing Lab to the Field: Exploring Innovations in Point-of-Care Diagnostics for the Rapid Detection and Management of Tropical Diseases in Resource-Limited Settings DOI Creative Commons
Abdullahi Tunde Aborode, Ridwan Olamilekan Adesola, Godfred Yawson Scott

et al.

Advances in Biomarker Sciences and Technology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

1
Introducing AVAC as an ultra-sensitive platform with broad dynamical range for high-throughput multiplexed biomarker detection using digital counting of plasmonic nanoparticles DOI Creative Commons
Virginia Cebrián, Valerio Pini, A. Thon

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 13, 2025

Accurate detection and quantification of biomarkers at ultra-low levels is critical for disease diagnosis effective treatment. Traditional technologies often lack the sensitivity, specificity, throughput, or multiplexing capacity required comprehensive diagnostics, providing only a subset these requirements. Here, we introduce AVAC, an automated optical technology rapid accurate biomarker with ultra-high sensitivity that significantly outperforms standard clinical assays. The core this digital counting plasmonic nanoparticles used as labels, enabling multiplexed, high-throughput biomarkers. Validation studies demonstrate AVAC's high accuracy, 98.2% specificity limits low 26 fg/mL HIV p24 protein range 160 to 850 pg/mL interleukin-6 (IL-6). supports multiplexed assays without compromising demonstrated by simultaneous three key associated cardiovascular disease. A spanning more than four orders magnitude ensures robust from concentrations, its ability analyze up 1,000 samples per hour provides throughput suitable large laboratories. With unique combination capabilities, versatile platform has significant potential advance biomarker-based diagnostics in research settings.

Language: Английский

Citations

1
Portable, Quantitative, Real-time Isothermal Nucleic Acid Amplification Test Using Microfluidic Device-Coupled UV-LED Photodiode Detector DOI
Natish Kumar,

Monika Kumari,

Devtulya Chander

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: unknown, P. 117194 - 117194

Published: Jan. 1, 2025

Language: Английский

Citations

0
Exploring the Diagnostic Landscape: Portable Aptasensors in Point-of-care Testing DOI
A. Mohan, Ipsita Roy

Analytical Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 115788 - 115788

Published: Jan. 1, 2025

Language: Английский

Citations

0
Multiplexed detection of respiratory virus RNA using optical pH sensors and injection-molded centrifugal microfluidics DOI Creative Commons

Gianmarco Domenico Suarez,

Yuki Yu Kiu Tang,

Steevanson Bayer

et al.

Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(3)

Published: Feb. 12, 2025

Abstract The application is demonstrated of injection-molded centrifugal microfluidic chips with integrated optical pH sensors for multiplexed detection respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A, and B RNA. generated sensitive fluorescent readouts from diagnostic reverse transcription loop-mediated isothermal amplification (RT-LAMP) reactions; limits influenzas A B, SARS-CoV-2 89, 245, 38 RNA copies per reaction, respectively, were attained. Results obtainable within 44 min 48 B. We implemented a data processing strategy based on numerical derivatives the fluorescence curves that allowed reliable, quantitative thresholds deciding reaction outcomes enabled 100% specificity. This work demonstrates utility microfluidics infectious disease diagnostics point-of-care applications. Graphical

Language: Английский

Citations

0
Polymerase Chain Reaction Chips for Biomarker Discovery and Validation in Drug Development DOI Creative Commons
Dang-Khoa Vo, Kieu The Loan Trinh

Micromachines, Journal Year: 2025, Volume and Issue: 16(3), P. 243 - 243

Published: Feb. 20, 2025

Polymerase chain reaction (PCR) chips are advanced, microfluidic platforms that have revolutionized biomarker discovery and validation because of their high sensitivity, specificity, throughput levels. These miniaturize traditional PCR processes for the speed precision nucleic acid detection relevant to advancing drug development. Biomarkers, which useful in helping explain disease mechanisms, patient stratification, therapeutic monitoring, hard identify validate due complexity biological systems limitations techniques. The challenges respond include high-throughput capabilities coupled with real-time quantitative analysis, enabling researchers novel biomarkers greater accuracy reproducibility. More recent design improvements further expanded functionality also digital multiplex technologies. Digital ideal quantifying rare biomarkers, is essential oncology infectious research. In contrast, enable simultaneous analysis multiple targets, therefore simplifying validation. Furthermore, single-cell made it possible detect at unprecedented resolution, hence revealing heterogeneity within cell populations. transforming development, target identification, efficacy assessment. They play a major role development companion diagnostics and, therefore, pave way personalized medicine, ensuring right receives treatment. While this tremendously promising technology has exhibited many regarding its scalability, integration other omics technologies, conformity regulatory requirements, still prevail. Future breakthroughs chip manufacturing, artificial intelligence, multi-omics applications will expand capabilities. not only be important acceleration but raising bar improving outcomes hence, global health care as these technologies continue mature.

Language: Английский

Citations

0