Опубликована: Янв. 1, 2024
Язык: Английский
Microorganisms, Год журнала: 2024, Номер 12(3), С. 430 - 430
Опубликована: Фев. 20, 2024
Next-generation probiotics (NGPs) represent an innovative group of beneficial bacteria that are currently undergoing research and development. NGPs designed not only for conventional use as foods or dietary supplements but also tailored pharmaceutical applications. Research indicates show therapeutic promise in addressing various chronic ailments. Offering multiple advantages over probiotics, present opportunities personalized probiotic therapies, involvement synthetic biology gene editing, participation combination targeted delivery methods, application settings. Our review discusses the potential effect NGPs, covering diverse trajectories including their identification, characterization, delivery. Furthermore, this elucidates influence on critical aspects human health, specifically, gut immune function, broader health outcomes. Mechanistic insights encompass production bioactive compounds, competitive interactions with pathogenic bacteria, modulation cell activity, reinforcement barrier. What is noteworthy current points out prevalent NGP strains sources, providing a highlight comprehensive framework understanding applications future benefits domain advanced therapeutics.
Язык: Английский
Процитировано
59
Process Biochemistry, Год журнала: 2025, Номер unknown
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
4
Molecular Biology Reports, Год журнала: 2024, Номер 51(1)
Опубликована: Фев. 25, 2024
Язык: Английский
Процитировано
13
Foods, Год журнала: 2025, Номер 14(7), С. 1148 - 1148
Опубликована: Март 26, 2025
Research in metagenomics and metaproteomics can reveal how microbiological interactions fermented foods contribute to their health benefits. This study examined three types of vegetables: a standard formulation, probiotic formulation with Lacticaseibacillus rhamnosus GG, polyphenol vitexin from Mung bean seed coat. Measurements were taken at day 0 (after 36 h fermentation room temperature) after 15 days. We applied 16S rRNA sequencing evaluate microbial diversity utilized LC-MS/MS investigate the proteomic profiles specific genera (Lactobacillus Weissella) species (Lacticaseibacillus Levilactobacillus brevis) lactic acid bacteria (LAB). All these taxa demonstrated significant relative abundance between days our metagenomic analysis. Our findings principal component analysis clustering categorically distinguished protein expression patterns various stages fermentation. By comparing samples 15, we identified proteins associated DNA replication repair mechanisms, including transcription elongation factor GreA, tRNA pseudouridine synthase B, helicases. also observed roles synthesis, which encompasses oxidoreductases aspartokinase. Furthermore, strong correlations across formulations antioxidant markers. In conclusion, results this decisively enhance understanding role related LAB foods, highlighting potential improve texture, flavor, nutritional quality,
Язык: Английский
Процитировано
1
Current Opinion in Systems Biology, Год журнала: 2023, Номер 36, С. 100471 - 100471
Опубликована: Авг. 19, 2023
The development of bacterial chassis to increase productivity and reduce industrial costs in value-added biochemical production has gained significant attention. Current efforts have focused on model bacteria, thus limiting their suitability produce specialized products. Therefore, there is a growing emphasis developing non-model expand the repertoire bioproducts. However, lack genetic information tools for bacteria remains challenging. In this review, we categorize introduce based characteristics relation target We also provide an overview trends genome-reduced enhance productivity. Furthermore, propose synthetic biology technologies that can be applied broad range bacteria.
Язык: Английский
Процитировано
18
Food Bioscience, Год журнала: 2024, Номер 61, С. 104714 - 104714
Опубликована: Июль 9, 2024
Язык: Английский
Процитировано
9
Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(50), С. 27679 - 27700
Опубликована: Ноя. 26, 2024
The burgeoning field of microbiome research has profoundly reshaped our comprehension human health, particularly highlighting the potential probiotics and fecal microbiota transplantation (FMT) as therapeutic interventions. While benefits traditional are well-recognized, efficacy mechanisms remain ambiguous, FMT's long-term effects still being investigated. Recent advancements in high-throughput sequencing have identified gut microbes with significant health benefits, paving way for next-generation (NGPs). These NGPs, engineered through synthetic biology bioinformatics, designed to address specific disease states enhanced stability viability. This review synthesizes current on NGP stability, challenges delivery, their applications preventing treating chronic diseases such diabetes, obesity, cardiovascular diseases. We explore physiological characteristics, safety profiles, action various strains while also addressing opportunities presented by integration into clinical practice. NGPs revolutionize microbiome-based therapies improve outcomes is immense, underscoring need further optimize ensure safety.
Язык: Английский
Процитировано
8
DNA, Год журнала: 2024, Номер 4(4), С. 427 - 454
Опубликована: Ноя. 14, 2024
Genetic engineering has revolutionized our ability to modify microorganisms for various applications in agriculture, medicine, and industry. This review examines recent advances genetic techniques bacteria, fungi, oomycetes, with a focus on CRISPR-Cas systems. In CRISPR-Cas9 enabled precise genome editing, enhancing antibiotic production metabolic engineering. For despite challenges associated their complex cell structures, CRISPR/Cas9 advanced the of enzymes secondary metabolites. significant plant pathogens, modified Agrobacterium-mediated transformation, CRISPR/Cas12a have contributed developing disease-resistant crops. provides comparative analysis efficiencies across these addresses ethical regulatory considerations. Future research directions include refining tools improve efficiency expand applicability non-model organisms. comprehensive overview highlights transformative potential microbiology its implications addressing global biotechnology.
Язык: Английский
Процитировано
7
Frontiers in Bioengineering and Biotechnology, Год журнала: 2024, Номер 12
Опубликована: Дек. 4, 2024
Over recent years, studies on microbiota research and synthetic biology have explored novel approaches microbial manipulation for therapeutic purposes. However, fragmented information is available this aspect with key insights scattered across various disciplines such as molecular biology, genetics, bioengineering, medicine. This review aims to the transformative potential of in advancing microbiome therapies, significant implications healthcare, agriculture, environmental sustainability. By merging computer science, engineering, allows precise design modification biological systems via cutting edge technologies like CRISPR/Cas9 gene editing, metabolic oligonucleotide synthesis, thus paving way targeted treatments personalized probiotics engineered microorganisms. The will also highlight vital role gut disorders caused by its dysbiosis suggesting microbiota-based therapies innovations biosensors real-time health monitoring, non-invasive diagnostic tools, automated bio foundries better outcomes. Moreover, challenges including genetic stability, safety, robust regulatory frameworks be discussed understand importance ongoing ensure safe effective interventions.
Язык: Английский
Процитировано
7
Food Reviews International, Год журнала: 2025, Номер unknown, С. 1 - 35
Опубликована: Янв. 30, 2025
Язык: Английский
Процитировано
1