Highlights in Science Engineering and Technology,
Journal Year:
2024,
Volume and Issue:
109, P. 204 - 224
Published: July 24, 2024
Probiotics
are
live
microorganisms
that
confer
health
benefits
to
the
host
when
administered
in
adequate
amounts.
This
review
provides
an
introduction
current
probiotics
field,
including
their
isolation
and
engineering,
some
common
genera,
actions,
applications
industry
public.
We
discuss
source
of
techniques
engineering
probiotics,
role
modulating
gut
health,
evidences
application
treating
gastrointestinal
disorders,
slightly
covers
outside
human.
also
discussed
actions
on
microbiota,
epithelial
environment,
human
gut-brain
axis.
Finally,
we
address
limitation
probiotic
products
application,
brings
up
future
research
directions
this
field.
The
purpose
paper
is
provide
introductory
Microorganisms,
Journal Year:
2024,
Volume and Issue:
12(3), P. 430 - 430
Published: Feb. 20, 2024
Next-generation
probiotics
(NGPs)
represent
an
innovative
group
of
beneficial
bacteria
that
are
currently
undergoing
research
and
development.
NGPs
designed
not
only
for
conventional
use
as
foods
or
dietary
supplements
but
also
tailored
pharmaceutical
applications.
Research
indicates
show
therapeutic
promise
in
addressing
various
chronic
ailments.
Offering
multiple
advantages
over
probiotics,
present
opportunities
personalized
probiotic
therapies,
involvement
synthetic
biology
gene
editing,
participation
combination
targeted
delivery
methods,
application
settings.
Our
review
discusses
the
potential
effect
NGPs,
covering
diverse
trajectories
including
their
identification,
characterization,
delivery.
Furthermore,
this
elucidates
influence
on
critical
aspects
human
health,
specifically,
gut
immune
function,
broader
health
outcomes.
Mechanistic
insights
encompass
production
bioactive
compounds,
competitive
interactions
with
pathogenic
bacteria,
modulation
cell
activity,
reinforcement
barrier.
What
is
noteworthy
current
points
out
prevalent
NGP
strains
sources,
providing
a
highlight
comprehensive
framework
understanding
applications
future
benefits
domain
advanced
therapeutics.
Foods,
Journal Year:
2025,
Volume and Issue:
14(7), P. 1148 - 1148
Published: March 26, 2025
Research
in
metagenomics
and
metaproteomics
can
reveal
how
microbiological
interactions
fermented
foods
contribute
to
their
health
benefits.
This
study
examined
three
types
of
vegetables:
a
standard
formulation,
probiotic
formulation
with
Lacticaseibacillus
rhamnosus
GG,
polyphenol
vitexin
from
Mung
bean
seed
coat.
Measurements
were
taken
at
day
0
(after
36
h
fermentation
room
temperature)
after
15
days.
We
applied
16S
rRNA
sequencing
evaluate
microbial
diversity
utilized
LC-MS/MS
investigate
the
proteomic
profiles
specific
genera
(Lactobacillus
Weissella)
species
(Lacticaseibacillus
Levilactobacillus
brevis)
lactic
acid
bacteria
(LAB).
All
these
taxa
demonstrated
significant
relative
abundance
between
days
our
metagenomic
analysis.
Our
findings
principal
component
analysis
clustering
categorically
distinguished
protein
expression
patterns
various
stages
fermentation.
By
comparing
samples
15,
we
identified
proteins
associated
DNA
replication
repair
mechanisms,
including
transcription
elongation
factor
GreA,
tRNA
pseudouridine
synthase
B,
helicases.
also
observed
roles
synthesis,
which
encompasses
oxidoreductases
aspartokinase.
Furthermore,
strong
correlations
across
formulations
antioxidant
markers.
In
conclusion,
results
this
decisively
enhance
understanding
role
related
LAB
foods,
highlighting
potential
improve
texture,
flavor,
nutritional
quality,
Current Opinion in Systems Biology,
Journal Year:
2023,
Volume and Issue:
36, P. 100471 - 100471
Published: Aug. 19, 2023
The
development
of
bacterial
chassis
to
increase
productivity
and
reduce
industrial
costs
in
value-added
biochemical
production
has
gained
significant
attention.
Current
efforts
have
focused
on
model
bacteria,
thus
limiting
their
suitability
produce
specialized
products.
Therefore,
there
is
a
growing
emphasis
developing
non-model
expand
the
repertoire
bioproducts.
However,
lack
genetic
information
tools
for
bacteria
remains
challenging.
In
this
review,
we
categorize
introduce
based
characteristics
relation
target
We
also
provide
an
overview
trends
genome-reduced
enhance
productivity.
Furthermore,
propose
synthetic
biology
technologies
that
can
be
applied
broad
range
bacteria.
DNA,
Journal Year:
2024,
Volume and Issue:
4(4), P. 427 - 454
Published: Nov. 14, 2024
Genetic
engineering
has
revolutionized
our
ability
to
modify
microorganisms
for
various
applications
in
agriculture,
medicine,
and
industry.
This
review
examines
recent
advances
genetic
techniques
bacteria,
fungi,
oomycetes,
with
a
focus
on
CRISPR-Cas
systems.
In
CRISPR-Cas9
enabled
precise
genome
editing,
enhancing
antibiotic
production
metabolic
engineering.
For
despite
challenges
associated
their
complex
cell
structures,
CRISPR/Cas9
advanced
the
of
enzymes
secondary
metabolites.
significant
plant
pathogens,
modified
Agrobacterium-mediated
transformation,
CRISPR/Cas12a
have
contributed
developing
disease-resistant
crops.
provides
comparative
analysis
efficiencies
across
these
addresses
ethical
regulatory
considerations.
Future
research
directions
include
refining
tools
improve
efficiency
expand
applicability
non-model
organisms.
comprehensive
overview
highlights
transformative
potential
microbiology
its
implications
addressing
global
biotechnology.
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2024,
Volume and Issue:
12
Published: Dec. 4, 2024
Over
recent
years,
studies
on
microbiota
research
and
synthetic
biology
have
explored
novel
approaches
microbial
manipulation
for
therapeutic
purposes.
However,
fragmented
information
is
available
this
aspect
with
key
insights
scattered
across
various
disciplines
such
as
molecular
biology,
genetics,
bioengineering,
medicine.
This
review
aims
to
the
transformative
potential
of
in
advancing
microbiome
therapies,
significant
implications
healthcare,
agriculture,
environmental
sustainability.
By
merging
computer
science,
engineering,
allows
precise
design
modification
biological
systems
via
cutting
edge
technologies
like
CRISPR/Cas9
gene
editing,
metabolic
oligonucleotide
synthesis,
thus
paving
way
targeted
treatments
personalized
probiotics
engineered
microorganisms.
The
will
also
highlight
vital
role
gut
disorders
caused
by
its
dysbiosis
suggesting
microbiota-based
therapies
innovations
biosensors
real-time
health
monitoring,
non-invasive
diagnostic
tools,
automated
bio
foundries
better
outcomes.
Moreover,
challenges
including
genetic
stability,
safety,
robust
regulatory
frameworks
be
discussed
understand
importance
ongoing
ensure
safe
effective
interventions.
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
72(50), P. 27679 - 27700
Published: Nov. 26, 2024
The
burgeoning
field
of
microbiome
research
has
profoundly
reshaped
our
comprehension
human
health,
particularly
highlighting
the
potential
probiotics
and
fecal
microbiota
transplantation
(FMT)
as
therapeutic
interventions.
While
benefits
traditional
are
well-recognized,
efficacy
mechanisms
remain
ambiguous,
FMT's
long-term
effects
still
being
investigated.
Recent
advancements
in
high-throughput
sequencing
have
identified
gut
microbes
with
significant
health
benefits,
paving
way
for
next-generation
(NGPs).
These
NGPs,
engineered
through
synthetic
biology
bioinformatics,
designed
to
address
specific
disease
states
enhanced
stability
viability.
This
review
synthesizes
current
on
NGP
stability,
challenges
delivery,
their
applications
preventing
treating
chronic
diseases
such
diabetes,
obesity,
cardiovascular
diseases.
We
explore
physiological
characteristics,
safety
profiles,
action
various
strains
while
also
addressing
opportunities
presented
by
integration
into
clinical
practice.
NGPs
revolutionize
microbiome-based
therapies
improve
outcomes
is
immense,
underscoring
need
further
optimize
ensure
safety.
