The actin cortex acts as a mechanical memory of morphology in confined migrating cells
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 5, 2024
Cell
migration
in
narrow
microenvironments
is
a
hallmark
of
numerous
physiological
processes,
involving
successive
cycles
confinement
and
release
that
drive
significant
morphological
changes.
However,
it
remains
unclear
whether
migrating
cells
can
retain
memory
their
past
states,
which
could
potentially
enhance
navigation
through
confined
spaces.
By
combining
cell
assays
on
standardized
microsystems
with
biophysical
modeling
biochemical
perturbations,
we
demonstrate
local
geometry
governs
these
switches,
thereby
facilitating
passage
long
gaps.
We
uncovered
long-term
events
cells,
states
correlated
across
transitions
actin
cortex
remodeling.
These
findings
suggest
mechanical
plays
an
active
role
migratory
potential
environments.
Язык: Английский
Feeling the force from within – new tools and insights into nuclear mechanotransduction
Journal of Cell Science,
Год журнала:
2025,
Номер
138(5)
Опубликована: Март 1, 2025
The
ability
of
cells
to
sense
and
respond
mechanical
signals
is
essential
for
many
biological
processes
that
form
the
basis
cell
identity,
tissue
development
maintenance.
This
process,
known
as
mechanotransduction,
involves
crucial
feedback
between
force
biochemical
signals,
including
epigenomic
modifications
establish
transcriptional
programs.
These
programs,
in
turn,
reinforce
properties
its
withstand
perturbation.
nucleus
has
long
been
hypothesized
play
a
key
role
mechanotransduction
due
direct
exposure
forces
transmitted
through
cytoskeleton,
receiving
cytoplasmic
central
function
gene
regulation.
However,
parsing
out
specific
contributions
from
those
surface
cytoplasm
remains
substantial
challenge.
In
this
Review,
we
examine
latest
evidence
on
how
regulates
both
via
nuclear
envelope
(NE)
epigenetic
machinery
elements
within
interior.
We
also
explore
establishing
memory,
characterized
by
mechanical,
transcriptomic
state
persists
after
stimuli
cease.
Finally,
discuss
current
challenges
field
present
technological
advances
are
poised
overcome
them.
Язык: Английский
Mechanomemory after short episodes of intermittent stresses induces YAP translocation via increasing F-actin
APL Bioengineering,
Год журнала:
2025,
Номер
9(2)
Опубликована: Апрель 18, 2025
How
forces
and
mechanics
influence
regulate
living
cells
remains
elusive.
Mechanomemory,
the
response
to
a
mechanical
perturbation
that
persists
after
is
removed,
believed
be
key
understanding
impact
of
on
cell
functions.
Recently,
our
lab
has
demonstrated
presence
mechanomemory
lasts
for
∼30
min
applying
external
stress
via
integrins.
Herein,
we
test
hypothesis
applications
short
intermittent
episodes
exert
long-term
effects
process
mechanotransduction.
An
Arginine-Glycine-Aspartic
acid
(RGD)-peptides-coated
4-μm
magnetic
bead
was
bound
integrin
receptors
apply
stresses
surface
Chinese
Hamster
Ovary
cell.
At
same
magnitude
frequency
(15
Pa
at
0.3
Hz),
multiple
cycles
externally
applied
2
or
10
with
15
intervals,
30
plus
load-free
interval
increased
nuclear
translocation
YAP
(Yes-Associated
Protein)
Ctgf
gene
expression,
like
by
60
continuous
stress,
but
did
not.
Short
durations
F-actin
in
cytoplasm,
which
coincided
elevated
translocation.
Inhibiting
actomyosin
not
microtubules
blocked
stress-induced
nucleus.
Cells
soft
substrates
translocate
more
than
stiff
load
release.
These
results
highlight
stresses-induced
cytoplasmic
biological
functions
Язык: Английский
Mechanobiology of 3D cell confinement and extracellular crowding
Biophysical Reviews,
Год журнала:
2024,
Номер
16(6), С. 833 - 849
Опубликована: Окт. 23, 2024
Cells
and
tissues
are
often
under
some
level
of
confinement,
imposed
by
the
microenvironment
neighboring
cells,
meaning
that
there
limitations
to
cell
size,
volume
changes,
fluid
exchanges.
3D
culture,
increasingly
used
for
both
single
cells
organoids,
inherently
impose
levels
confinement
absent
in
2D
systems.
It
is
thus
key
understand
how
different
influences
survival,
function,
fate.
well
known
mechanical
properties
microenvironment,
such
as
stiffness
stress
relaxation,
important
activating
mechanosensitive
pathways,
these
responsive
conditions.
In
this
review,
we
look
at
low,
intermediate,
high
modulate
activation
mechanobiology
tumor
spheroids,
with
a
specific
focus
on
microwells,
elastic,
or
viscoelastic
scaffolds.
addition,
confining
can
drastically
limit
cellular
communication
healthy
diseased
tissues,
due
extracellular
crowding.
We
discuss
potential
implications
crowding
molecular
transport,
matrix
deposition,
transport.
Understanding
sense
respond
various
should
inform
design
engineered
matrices
recapitulate
physical
tissues.
Язык: Английский
Advances and Challenges in the Use of Liquid Biopsy in Gynaecological Oncology
Heliyon,
Год журнала:
2024,
Номер
10(20), С. e39148 - e39148
Опубликована: Окт. 1, 2024
Язык: Английский
Confinement-sensitive volume regulation dynamics via high-speed nuclear morphological measurements
Proceedings of the National Academy of Sciences,
Год журнала:
2024,
Номер
121(52)
Опубликована: Дек. 19, 2024
Diverse
tissues
in
vivo
present
varying
degrees
of
confinement,
constriction,
and
compression
to
migrating
cells
both
homeostasis
disease.
The
nucleus
particular
is
subjected
external
forces
by
the
physical
environment
during
confined
migration.
While
many
systems
have
been
developed
induce
nuclear
deformation
analyze
resultant
functional
changes,
much
remains
unclear
about
dynamic
volume
regulation
confinement
due
limitations
time
resolution
difficulty
imaging
PDMS-based
microfluidic
chips.
Standard
volumetric
measurement
relies
on
confocal
microscopy,
which
suffers
from
high
phototoxicity,
slow
speed,
limited
throughput,
artifacts
fast-moving
cells.
To
address
this,
we
a
form
double
fluorescence
exclusion
designed
function
at
interface
microchannel-based
PDMS
sidewalls,
that
can
track
cellular
dynamics
By
verifying
vertical
symmetry
nuclei
obtained
computational
estimates
surface
area.
We
then
tracked
area
under
physiological
exceeding
30
frames
per
minute.
find
self-induced
entrance
into
cell
rapidly
expands
its
until
threshold
reached,
followed
rapid
decrease
volume.
next
used
osmotic
shock
as
tool
alter
found
response
hypo-osmotic
does
not
follow
classical
scaling
laws,
suggesting
expansion
potential
envelope
might
be
constraining
factor
confining
environments
vivo.
Язык: Английский
Advances and Challenges in the Use of Liquid Biopsy in Gynaecological Oncology
Опубликована: Янв. 1, 2024
Язык: Английский
Cell confinement by micropatterning induces phenotypic changes in cancer-associated fibroblasts
Acta Biomaterialia,
Год журнала:
2024,
Номер
192, С. 61 - 76
Опубликована: Дек. 4, 2024
Язык: Английский
Механізми та маркери метастазування при карциномах щитоподібної залози. Огляд літератури та власних даних (частина 1)
Endokrynologia,
Год журнала:
2024,
Номер
29(3), С. 283 - 293
Опубликована: Окт. 30, 2024
Резюме.
Огляд
літератури
присвячено
маркерам
та
механізмам
утворення
метастазів.
Акцент
робиться
на
відносно
маловивчених
процесах
–
ролі
жорсткості
пухлини
її
оточення,
участі
в
цих
асоційованих
із
пухлиною
фібробластів;
формування
преметастатичних
ніш,
переходу
дисемінованих
клітин
у
сплячий
стан
та,
особливо,
механізмам,
які
провокують
вихід
метастазів
цього
стану,
що
має
суттєве
практичне
значення.
Встановлено,
сайти
майбутніх
не
є
пасивними
приймачами
ракових
клітин,
а
вибірково
й
активно
модифікуються
первинною
ще
до
того,
як
відбулося
метастатичне
поширення.
Пухлини
індукують
мікрооточення
віддалених
органах,
яке
буде
сприяти
виживанню
росту
пухлинних
після
їх
засіву
метастатичні
сайти.
Підкреслюється
важливість
визначення
факторів,
сприяють
формуванню
ніш
при
карциномах
щитоподібної
залози
(ЩЗ).
Іноді,
замість
підвищують
ефективність
метастазування,
можуть
виникати
спеціалізовані
мікросередовища,
яких
пухлинні
клітини
виживають
стані
спокою.
Зараз
з’ясовуються
клітинні
молекулярні
складові
спокою
клітин.
Наведені
фактори,
спровокувати
зі
стану
Головна
увага
приділяється
метастазування
ЩЗ
трансформуючому
фактору
бета
(transforming
growth
factor
beta,
TGF-β),
матриксним
металопротеазам
(matrix
metalloproteinases,
ММРs),
фібробластів
19
(fibroblast
factors
19,
FGF19),
індукованому
гіпоксією
1α
(hypoxia-inducible
factors,
HIF-1α),
судинному
ендотеліальному
(vascular
endothelial
factor,
VEGF),
фібронектину,
інтегринам,
альфа-актину
гладких
м’язів
(α-smooth
muscle
actin,
α-SMA),
синдеканам,
кіназам
сімейства
Src,
осі
CXCL12/CXCR4,
стовбурових
ін.
Особливий
інтерес
викликають
маркери,
можна
виявити
плазмі
крові
методом
тонкоголкової
біопсії
доопераційний
період.
The first embryo, the origin of cancer and animal phylogeny. V. Cancer stem cells as the unifying biomechanical principle between embryology and oncology
Mechanobiology in Medicine,
Год журнала:
2024,
Номер
unknown, С. 100110 - 100110
Опубликована: Дек. 1, 2024
Язык: Английский