Fat Body—Multifunctional Insect Tissue

Insects, Год журнала: 2021, Номер 12(6), С. 547 - 547

Опубликована: Июнь 11, 2021

The biodiversity of useful organisms, e.g., insects, decreases due to many environmental factors and increasing anthropopressure. Multifunctional tissues, such as the fat body, are key elements in proper functioning invertebrate organisms resistance factors. body is center metabolism, integrating signals, controlling molting metamorphosis, synthesizing hormones that control whole synthesis immune system proteins. In cells, lipids, carbohydrates proteins substrates products pathways can be used for energy production, accumulate reserves, mobilize at appropriate stage life (diapause, flight), determining survival an individual. main tissue responsible innate acquired humoral immunity. produces bactericidal polypeptides, i.e., lysozyme. also important early stages insect’s production vitellogenin, yolk protein needed development oocytes. Although a lot information available on its structure biochemistry, interesting research topic which much still discovered.

Язык: Английский

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Imidacloprid disrupts larval molting regulation and nutrient energy metabolism, causing developmental delay in honey bee Apis mellifera

eLife, Год журнала: 2024, Номер 12

Опубликована: Март 11, 2024

Imidacloprid is a global health threat that severely poisons the economically and ecologically important honeybee pollinator, Apis mellifera . However, its effects on developing bee larvae remain largely unexplored. Our pilot study showed imidacloprid causes developmental delay in larvae, but underlying toxicological mechanisms incompletely understood. In this study, we exposed to at environmentally relevant concentrations of 0.7, 1.2, 3.1, 377 ppb. There was marked dose-dependent larval development, characterized by reductions body mass, width, growth index. did not affect survival food consumption. The primary induced elevated (377 ppb) included inhibition neural transmission gene expression, induction oxidative stress, gut structural damage, apoptosis, regulatory hormones genes, suppression expression levels involved proteolysis, amino acid transport, protein synthesis, carbohydrate catabolism, phosphorylation, glycolysis energy production. addition, found may use antioxidant defenses P450 detoxification mitigate imidacloprid. Ultimately, provides first evidence can development disrupting molting regulation limiting metabolism utilization dietary nutrients energy. These findings have broader implications for studies assessing pesticide hazards other juvenile animals.

Язык: Английский

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Drosophila as a Model Organism to Study Basic Mechanisms of Longevity

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(19), С. 11244 - 11244

Опубликована: Сен. 24, 2022

The spatio-temporal regulation of gene expression determines the fate and function various cells tissues and, as a consequence, correct development functioning complex organisms. Certain mechanisms activity provide adequate cell responses to changes in environmental factors. Aside from disorders that lead pathologies, alterations particular genes were shown significantly decrease or increase lifespan wide range organisms yeast human. Drosophila fruit fly is an ideal model system explore longevity aging due low cost, easy handling maintenance, large number progeny per adult, short life cycle lifespan, relatively paralogous genes, high evolutionary conservation epigenetic signalling pathways, availability tools modulate vivo. Here, we focus on organization evolutionarily conserved signaling pathways whose components influence process interconnections these with regulation.

Язык: Английский

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Mmp-induced fat body cell dissociation promotes pupal development and moderately averts pupal diapause by activating lipid metabolism

Proceedings of the National Academy of Sciences, Год журнала: 2022, Номер 120(1)

Опубликована: Дек. 27, 2022

In Lepidoptera and Diptera, the fat body dissociates into single cells in nondiapause pupae, but it does not dissociate diapause pupae until termination. Using cotton bollworm, Helicoverpa armigera, as a model of pupal insects, we illustrated catalytic mechanism physiological importance cell dissociation regulating development diapause. cathepsin L (CatL) activates matrix metalloproteinases (Mmps) that degrade extracellular proteins cause dissociation. Mmp-induced lipid metabolism through transcriptional regulation, resulting energetic supplies increase brain metabolic activity (i.e., mitochondria respiration insulin signaling) thus promote development. low activities CatL Mmps prevent from occurring, maintaining Importantly, demonstrated by chemical inhibitor treatments CRISPR-mediated gene knockouts, Mmp inhibition delayed moderately increased incidence diapause, while stimulation promoted averted This study advances our recent understanding biology insect regulation.

Язык: Английский

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NPFR regulates the synthesis and metabolism of lipids and glycogen via AMPK: Novel targets for efficient corn borer management

International Journal of Biological Macromolecules, Год журнала: 2023, Номер 247, С. 125816 - 125816

Опубликована: Июль 13, 2023

Язык: Английский

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Comparative Fecal Metabolomes of Silkworms Being Fed Mulberry Leaf and Artificial Diet

Insects, Год журнала: 2020, Номер 11(12), С. 851 - 851

Опубликована: Ноя. 30, 2020

Metabonomics accurately monitors the precise metabolic responses to various dietary patterns. Metabolic profiling allows simultaneous measurement of fecal metabolites whose concentrations may be affected by food intake. In this study, we analyzed metabolomes silkworm (Bombyx mori) larvae reared on fresh mulberry leaves and artificial diets. 57 differentially expressed were identified gas chromatography–mass spectrometry. Of these, 39 up-regulated 18 downregulated in leaf meal group. Most amino acids, carbohydrates lipids associated with physical development silk protein biosynthesis enriched silkworms leaves. contrast, urea, citric acid, D-pinitol, D-(+)-cellobiose N-acetyl glucosamine levels relatively higher feeding The findings study help clarify association between diet profiling.

Язык: Английский

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Juvenile hormone membrane signaling phosphorylates USP and thus potentiates 20-hydroxyecdysone action in Drosophila

Science Bulletin, Год журнала: 2021, Номер 67(2), С. 186 - 197

Опубликована: Июнь 24, 2021

Язык: Английский

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The Nrf2-Keap1 pathway is activated by steroid hormone signaling to govern neuronal remodeling

Cell Reports, Год журнала: 2021, Номер 36(5), С. 109466 - 109466

Опубликована: Авг. 1, 2021

The evolutionarily conserved Nrf2-Keap1 pathway is a key antioxidant response that protects cells/organisms against detrimental effects of oxidative stress. Impaired Nrf2 function associated with cancer and neurodegenerative diseases in humans. However, the developing nervous systems has not been established. Here we demonstrate cell-autonomous role pathway, composed CncC/Nrf2, Keap1, MafS, governing neuronal remodeling during Drosophila metamorphosis. signaling activated downstream steroid hormone ecdysone. Mechanistically, via cytoplasmic-to-nuclear translocation CncC an importin- ecdysone-signaling-dependent manner. Moreover, regulates dendrite pruning independent its canonical acting instead through proteasomal degradation. This study reveals epistatic link between demonstrates antioxidant-independent but proteasome-dependent remodeling.

Язык: Английский

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Development of Botanical Pesticides: Exploration on the Phenotype of Vestigial Wings of Insect Pests Induced by Plant Natural Products or Their Derivatives by Blocking Tyrosine Phosphorylation of Insulin Receptor 1

Journal of Agricultural and Food Chemistry, Год журнала: 2022, Номер 70(7), С. 2117 - 2126

Опубликована: Фев. 9, 2022

Unlike faster-acting conventional insecticides, some botanical insecticides exhibit growth inhibitory activity against insect pests. One of the distinguishing features appears to be in malformed moths with vestigial wings. However, molecular mechanism underlying wings pests induced by plant natural products or their derivatives is still elusive. In this work, based upon phenotype Mythimna separata Walker (as a model pest) podophyllotoxin derivative 2a compound), we found that compound not only resulted 22.1% but also significantly decreased fecundity vestigial-winged female P generation; trait caused generation can inherited F1 may target insulin receptor 1 (InR1), suppress InR1 mRNA level, and block InR1-pY1229 InR1-pY1233/1234 phosphorylation levels tissue-specific manner "head/thorax/wing tissues". Notably, induce Spodoptera frugiperda (another seriously harmful migratory lepidoptera pest). It noteworthy used as new kind receptors for design novel green insecticides.

Язык: Английский

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Baculovirus protein kinase 1 activates AMPK-protein phosphatase 5 axis to hijack transcription factor EB for self-proliferation

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 139884 - 139884

Опубликована: Янв. 1, 2025

Язык: Английский

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