Pharmaceuticals,
Год журнала:
2024,
Номер
17(11), С. 1537 - 1537
Опубликована: Ноя. 16, 2024
Sitafloxacin
is
a
4th
generation
fluoroquinolone
antibiotic
with
broad
activity
against
wide
range
of
Gram-negative
and
Gram-positive
bacteria.
It
approved
in
Japan
used
to
treat
pneumonia
urinary
tract
infections
(UTIs)
as
well
other
upper
lower
respiratory
infections,
genitourinary
oral
otitis
media.
Compared
fluoroquinolones,
sitafloxacin
displays
low
minimal
inhibitory
concentration
(MIC)
for
many
bacterial
species
but
also
anaerobes,
intracellular
bacteria,
persisters.
Furthermore,
it
has
shown
strong
biofilms
Science,
Год журнала:
2021,
Номер
372(6547), С. 1169 - 1175
Опубликована: Июнь 10, 2021
Emergent
resistance
to
all
clinical
antibiotics
calls
for
the
next
generation
of
therapeutics.
Here
we
report
an
effective
antimicrobial
strategy
targeting
bacterial
hydrogen
sulfide
(H2S)-mediated
defense
system.
We
identified
cystathionine
γ-lyase
(CSE)
as
primary
generator
H2S
in
two
major
human
pathogens,
Staphylococcus
aureus
and
Pseudomonas
aeruginosa,
discovered
small
molecules
that
inhibit
CSE.
These
inhibitors
potentiate
bactericidal
against
both
pathogens
vitro
mouse
models
infection.
CSE
also
suppress
tolerance,
disrupting
biofilm
formation
substantially
reducing
number
persister
bacteria
survive
antibiotic
treatment.
Our
results
establish
a
multifunctional
factor
drug
target
versatile
enhancers.
PLoS Biology,
Год журнала:
2021,
Номер
19(4), С. e3001194 - e3001194
Опубликована: Апрель 19, 2021
Persisters
represent
a
small
subpopulation
of
non-
or
slow-growing
bacterial
cells
that
are
tolerant
to
killing
by
antibiotics.
Despite
their
prominent
role
in
the
recalcitrance
chronic
infections
antibiotic
therapy,
mechanism
formation
has
remained
elusive.
We
show
sorted
Escherichia
coli
with
low
levels
energy-generating
enzymes
better
able
survive
killing.
Using
microfluidics
time-lapse
microscopy
and
fluorescent
reporter
for
vivo
ATP
measurements,
we
find
level
survives
ampicillin.
propose
these
formed
stochastically
as
result
fluctuations
abundance
components.
These
findings
point
general
“low
energy”
persister
formation.
Nature Communications,
Год журнала:
2025,
Номер
16(1)
Опубликована: Янв. 26, 2025
Recalcitrant
bacterial
infections
can
be
caused
by
various
types
of
dormant
bacteria,
including
persisters
and
viable
but
nonculturable
(VBNC)
cells.
Despite
their
clinical
importance,
we
know
fairly
little
about
dormancy
development
recovery.
Previously,
established
a
correlation
between
protein
aggregation
in
Escherichia
coli.
Here,
present
further
support
for
direct
relationship
both.
Our
experiments
demonstrate
that
aggregates
progressively
sequester
proteins
involved
energy
production,
thereby
likely
causing
ATP
depletion
dormancy.
Furthermore,
structural
features
determine
the
cell's
ability
to
exit
resume
growth.
Proteins
were
shown
first
assemble
liquid-like
condensates
solidify
over
time.
This
liquid-to-solid
phase
transition
impedes
aggregate
dissolution,
preventing
growth
resumption.
data
model
which
structure,
rather
than
cellular
activity,
marks
from
persister
VBNC
state.
are
often
authors
explore
how
composition
structure
affect
this
Critical Reviews in Environmental Science and Technology,
Год журнала:
2021,
Номер
52(20), С. 3651 - 3688
Опубликована: Июнь 4, 2021
Disinfection
technologies,
especially
light-based
disinfection,
have
undergone
tremendous
development
and
innovation,
but
this
treatment
can
cause
bacteria
to
enter
viable
nonculturable
(VBNC)
state.
Due
their
strong
tolerance,
VBNC
cannot
be
completely
removed
by
disinfection
thereby
posing
a
potential
risk
for
antibiotic
resistance
gene
(ARG)
transfer.
Therefore,
better
understand
interpret
transfer
of
ARGs,
article
systematically
reviewed
changes
in
morphology,
physiology
virulence
after
entering
In
addition,
quantitative
detection
methods
bacteria,
such
as
cell
membrane
integrity-mediated
LIVE/DEAD
Baclight
assay,
qPCR-based
assays,
phage-based
methods,
concluding
that
there
is
still
lack
in-situ
real-time
bacteria.
Health
risks
environmental
application
value
were
then
valuated,
with
data
indicating
great
the
domain
microbial
utilization.
Furthermore,
induction
conditions
(especially
disinfection)
formation
mechanisms
highlighted.
Formation
mainly
involve
stringent
response,
general
stress
response
system
toxin-antitoxin
(TA)
system.
Moreover,
horizontal
(HGT)
ARGs
during
induced
was
evaluated.
It
found
may
transferred
through
conjugation,
transformation
transduction.
Finally,
current
deficiencies
future
challenges
those
influenced
summarized.
This
review
provides
new
insights
into
mechanisms,
applications
ARG
Microbiology Spectrum,
Год журнала:
2022,
Номер
10(1)
Опубликована: Фев. 23, 2022
By
their
capacity
to
survive
antibiotic
pressure
and
regrow
give
rise
a
susceptible
population
once
this
is
relieved,
intracellular
persisters
of
S.
aureus
may
contribute
explain
therapeutic
failures
recurrent
infections.
Here,
we
show
that
the
level
dormancy
subsequent
resuscitate
from
resting
state
are
dependent
on
oxidative
stress
in
host
cells
where
bacteria
survive.
Cell Host & Microbe,
Год журнала:
2024,
Номер
32(6), С. 852 - 862
Опубликована: Июнь 1, 2024
Antibiotic
resistance,
typically
associated
with
genetic
changes
within
a
bacterial
population,
is
frequent
contributor
to
antibiotic
treatment
failures.
persistence
and
tolerance,
which
we
collectively
term
recalcitrance,
represent
transient
phenotypic
in
the
population
that
prolong
survival
presence
of
lethal
concentrations
antibiotics.
recalcitrance
challenging
detect
investigate-traditionally
studied
under
vitro
conditions,
our
understanding
during
infection
its
contribution
failure
limited.
Recently,
significant
progress
has
been
made
study
antibiotic-recalcitrant
populations
pathogenic
species,
including
Mycobacterium
tuberculosis,
Staphylococcus
aureus,
Salmonella
enterica,
Yersiniae,
context
host
environment.
Despite
diversity
these
pathogens
models,
shared
signals
responses
promote
common
features
vulnerabilities
persisters
tolerant
bacteria
have
emerged.
These
will
be
discussed
here,
along
toward
developing
therapeutic
interventions
better
treat
recalcitrant
pathogens.
