Physiological roles of hydrogen sulfide in mammalian cells, tissues, and organs

Physiological Reviews, Год журнала: 2022, Номер 103(1), С. 31 - 276

Опубликована: Апрель 18, 2022

Over the last two decades, hydrogen sulfide (H2S) has emerged as an endogenous regulator of a broad range physiological functions. H2S belongs to class molecules known gasotransmitters, which typically include nitric oxide (NO) and carbon monoxide (CO). Three enzymes are recognized sources in various cells tissues: cystathionine γ-lyase (CSE), β-synthase (CBS), 3-mercaptopyruvate sulfurtransferase (3-MST). The present article reviews regulation these well pathways their enzymatic nonenzymatic degradation elimination. multiple interactions with other labile (e.g., NO) reactive oxygen species also outlined. Next, biological targets signaling outlined, special reference or oxidative posttranscriptional modification (persulfidation sulfhydration) proteins effect on channels intracellular second messenger pathways, gene transcription translation, cellular bioenergetics metabolism. pharmacological molecular tools currently available study physiology reviewed, including utility limitations. In subsequent sections, role functions is membrane potential, endo- exocytosis, cell organelles (endoplasmic reticulum, Golgi, mitochondria), movement, cycle, differentiation, aspects regulated death. roles types organ systems overviewed, red blood cells, immune central peripheral nervous (with focus neuronal transmission, learning, memory formation), vascular function (including angiogenesis its specialized cerebrovascular, renal, pulmonary beds) senses, vision, hearing, taste smell, pain-sensing. Finally, (lung, heart, liver, kidney, urogenital organs, reproductive system, bone cartilage, skeletal muscle, endocrine organs) presented, aging) but extending some common pathophysiological conditions. From data, wide array significant all emerges characteristic bell-shaped biphasic effects highlighted. addition, key aspects, debated areas, future research translational areas identified.

Язык: Английский

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One‐Step Synthesis of Multifunctional Chitosan Hydrogel for Full‐Thickness Wound Closure and Healing

Advanced Healthcare Materials, Год журнала: 2021, Номер 11(4)

Опубликована: Ноя. 17, 2021

Multifunctional hydrogel as a sealant or wound dressing with high adhesiveness and excellent antibacterial activity is highly desirable in clinical applications. In this contribution, one-step synthetic based on quaternized chitosan (QCS), tannic acid (TA), ferric iron (Fe(III)) developed for skin incision closure Staphylococcus aureus (S. aureus)-infected healing. system, the ionic bonds hydrogen between QCS TA form main backbone of hydrogel, metal coordination Fe(III) (catechol-Fe) endow (near-infrared light) NIR-responsive photothermal property, these multiple dynamic physical crosslinks enable QCS/TA/Fe flexible self-healing ability injectability. Moreover, possesses superior antioxidant, anti-inflammatory, hemostasis, biocompatibility. Also, it safe vital organs. The data from mouse model infected full-thickness presented effectiveness acceleration healing process by multifunctional highlighting its great potential management.

Язык: Английский

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The Mechanism of Bacterial Resistance and Potential Bacteriostatic Strategies

Antibiotics, Год журнала: 2022, Номер 11(9), С. 1215 - 1215

Опубликована: Сен. 8, 2022

Bacterial drug resistance is rapidly developing as one of the greatest threats to human health. Bacteria will adopt corresponding strategies crack inhibitory effect antibiotics according antibacterial mechanism antibiotics, involving mutation target, secreting hydrolase, and discharging out cells through an efflux pump, etc. In recent years, bacteria are found constantly evolve new mechanisms including target protective protein, changes in cell morphology, so on, endowing them with multiple defense systems against leading emergence multi-drug resistant (MDR) unavailability drugs clinics. Correspondingly, researchers attempt uncover mystery bacterial develop more convenient effective strategies. Although traditional still play a significant role treatment diseases caused by sensitive pathogenic bacteria, they gradually lose efficacy MDR bacteria. Therefore, highly compounds, such phage therapy CRISPER-Cas precision therapy, gaining increasing amount attention, considered be treatments moist potential regard future. this review, nine identified summarized, which enhance retention rate under action promote distribution drug-resistant (DRB) population. Afterwards, three kinds methods introduced, compounds exhibit broad application prospects different mechanisms, has been successfully applied infectious super technology can edit genes at gene level, high accuracy flexibility. These provide options for clinical treatment, greatly alleviate current crisis.

Язык: Английский

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Multifunctional hydrogel with reactive oxygen species scavenging and photothermal antibacterial activity accelerates infected diabetic wound healing

Acta Biomaterialia, Год журнала: 2022, Номер 155, С. 199 - 217

Опубликована: Ноя. 17, 2022

Язык: Английский

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Design of stretchable and self-powered sensing device for portable and remote trace biomarkers detection

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Авг. 26, 2023

Timely and remote biomarker detection is highly desired in personalized medicine health protection but presents great challenges the devices reported so far. Here, we present a cost-effective, flexible self-powered sensing device for H2S analysis various application scenarios based on structure of galvanic cells. The mechanism attributed to change electrode potential resulting from chemical adsorption gas molecules surfaces. Intrinsically stretchable organohydrogels are used as solid-state electrolytes enable stable long-term operation under stretching deformation or environments. open-circuit exhibits high sensitivity, low limit, excellent selectivity H2S. Its non-invasive halitosis diagnosis identification meat spoilage demonstrated, emerging commercial value portable medical electronics food security. A wireless sensory system has also been developed monitoring with participation Bluetooth cloud technologies. This work breaks through shortcomings traditional chemiresistive sensors, offering direction theoretical foundation designing wearable sensors catering other stimulus requirements.

Язык: Английский

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Fast bacterial growth reduces antibiotic accumulation and efficacy

eLife, Год журнала: 2022, Номер 11

Опубликована: Июнь 7, 2022

Phenotypic variations between individual microbial cells play a key role in the resistance of pathogens to pharmacotherapies. Nevertheless, little is known about cell individuality antibiotic accumulation. Here, we hypothesise that phenotypic diversification can be driven by fundamental cell-to-cell differences drug transport rates. To test this hypothesis, employed microfluidics-based single-cell microscopy, libraries fluorescent probes and mathematical modelling. This approach allowed us rapidly identify variants avoid accumulation within populations Escherichia coli , Pseudomonas aeruginosa Burkholderia cenocepacia, Staphylococcus aureus . Crucially, found fast growing macrolide survive treatment without genetic mutations. These findings are contrast with current consensus cellular dormancy slow metabolism underlie bacterial survival antibiotics. Our results also show display significantly higher expression ribosomal promoters before compared variants. Drug-free active ribosomes facilitate essential processes these fast-growing variants, including efflux reduce We used new knowledge eradicate displayed low through chemical manipulation their outer membrane inspiring avenues overcome failures.

Язык: Английский

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Multimodal nanoimmunotherapy engages neutrophils to eliminate Staphylococcus aureus infections

Nature Nanotechnology, Год журнала: 2024, Номер 19(7), С. 1032 - 1043

Опубликована: Апрель 17, 2024

Язык: Английский

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From the soil to the clinic: the impact of microbial secondary metabolites on antibiotic tolerance and resistance

Nature Reviews Microbiology, Год журнала: 2021, Номер 20(3), С. 129 - 142

Опубликована: Сен. 16, 2021

Язык: Английский

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Prospects for Antibacterial Discovery and Development

Journal of the American Chemical Society, Год журнала: 2021, Номер 143(50), С. 21127 - 21142

Опубликована: Дек. 3, 2021

The rising prevalence of multidrug-resistant bacteria is an urgent health crisis that can only be countered through renewed investment in the discovery and development antibiotics. There no panacea for antibacterial resistance crisis; instead, a multifaceted approach called for. In this Perspective we make case that, face evolving clinical needs enabling technologies, numerous validated targets associated lead molecules deserve second look. At same time, many worthy lack good leads despite harboring druggable active sites. Creative inspired techniques buoy efforts; while soil screening efforts frequently to antibiotic rediscovery, researchers have found success searching new by studying underexplored ecological niches or leveraging abundance available data from genome mining efforts. judicious use "polypharmacology" (i.e., ability drug alter activities multiple targets) also provide opportunities, as continued search inhibitors enzymes with capacity breathe life into old We conclude highlighting pharmacoeconomic models making ones.

Язык: Английский

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Emerging roles of cystathionine β-synthase in various forms of cancer

Redox Biology, Год журнала: 2022, Номер 53, С. 102331 - 102331

Опубликована: Май 10, 2022

The expression of the reverse transsulfuration enzyme cystathionine-β-synthase (CBS) is markedly increased in many forms cancer, including colorectal, ovarian, lung, breast and kidney, while other cancers (liver cancer glioma) it becomes downregulated. According to clinical database data high-CBS-expressor (e.g. colon or ovarian cancer), high CBS typically predicts lower survival, low-CBS-expressor liver low associated with survival. In high-CBS expressing tumor cells, CBS, its product hydrogen sulfide (H2S) serves as a bioenergetic, proliferative, cytoprotective stemness factor; also supports angiogenesis epithelial-to-mesenchymal transition microenvironment. current article reviews various tumor-cell-supporting roles CBS/H2S axis expressor overviews anticancer effects silencing pharmacological inhibition models vitro vivo; outlines potential approaches for biomarker identification, support future targeted therapies based on inhibition.

Язык: Английский

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