Rationally Designed Novel Antimicrobial Peptides Targeting Chitin Synthase for Combating Soybean Phytophthora Blight DOI Open Access

Yue Ran,

Kiran Shehzadi,

Jianhua Liang

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(6), С. 3512 - 3512

Опубликована: Март 20, 2024

Soybean phytophthora blight is a severe menace to global agriculture, causing annual losses surpassing USD 1 billion. Present crop loss mitigation strategies primarily rely on chemical pesticides and disease-resistant breeding, frequently surpassed by the pathogens' quick adaptive evolution. In this urgent scenario, our research delves into innovative antimicrobial peptides characterized low drug resistance environmental friendliness. Inhibiting chitin synthase gene activity in Phytophthora sojae impairs vital functions such as growth sporulation, presenting an effective method reduce its pathogenic impact. study, we screened 16 previously tested evaluate their effects against using structure-guided design, which involves molecular docking, saturation mutagenesis, dynamics, toxicity prediction. The silico analysis identified AMP_04 with potential inhibitory sojae's synthase. Through three rounds of pin-pointed most triple mutant, TP (D10K, G11I, S14L). Molecular dynamic simulations revealed TP's stability synthase-TP complex transmembrane mechanism, employing all-atom force field. Our findings demonstrate efficacy occupying substrate-binding pocket translocation catalytic channel. Effective inhibition enzyme can be achieved. Specifically, mutant demonstrates enhanced potency decreased relative wild-type AMP_04, utilizing mechanism akin barrel-stave model during membrane translocation. Collectively, study provides new strategy that could used potent agent combatting soybean blight, contributing sustainable agricultural practices.

Язык: Английский

Advancing horizons in vegetable cultivation: a journey from ageold practices to high-tech greenhouse cultivation—a review DOI Creative Commons
Nazir Ahmed, Baige Zhang,

Lansheng Deng

и другие.

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Апрель 15, 2024

Vegetable cultivation stands as a pivotal element in the agricultural transformation illustrating complex interplay between technological advancements, evolving environmental perspectives, and growing global demand for food. This comprehensive review delves into broad spectrum of developments modern vegetable practices. Rooted historical traditions, our exploration commences with conventional methods traces progression toward contemporary practices emphasizing critical shifts that have refined techniques outcomes. A significant focus is placed on evolution seed selection quality assessment underlining importance treatments enhancing both germination plant growth. Transitioning from seeds to soil, we investigate transformative journey traditional soil-based adoption soilless cultures utilization sustainable substrates like biochar coir. The also examines controls highlighting use advanced greenhouse technologies artificial intelligence optimizing growth conditions. We underscore increasing sophistication water management strategies irrigation systems intelligent moisture sensing. Additionally, this paper discusses intricate aspects precision fertilization, integrated pest management, expanding influence regulators cultivation. special segment dedicated innovations, such integration drones, robots, state-of-the-art digital monitoring systems, process. While acknowledging these realistically addresses challenges economic considerations involved adopting cutting-edge technologies. In summary, not only provides guide current state but serves forward-looking reference role continuous research anticipation future field.

Язык: Английский

Процитировано

16
The recent possible strategies for breeding ultraviolet-B-resistant crops DOI Creative Commons
Gideon Sadikiel Mmbando

Heliyon, Год журнала: 2024, Номер 10(6), С. e27806 - e27806

Опубликована: Март 1, 2024

The sensitivity of crops to ultraviolet B (UVB, 280–315 nm) radiation varies significantly. Plants' UVB is heavily influenced by the activity enzyme cyclobutane pyrimidine dimer (CPD) photolyase, which fixes UVB-induced CPDs. Crops grown in tropical areas with high level radiation, like O. glaberrima from Africa and sativa ssp. indica rice Bengal, are more sensitive could suffer as a result rising levels on earth's surface. Therefore, creating that can withstand crucial regions. There is, however, little information current techniques for breeding UVB-resistant plants. most recent producing presented this review. use DNA methylation, boosting antioxidant system, regulating expression micro-RNA396, overexpressing CPD photolyase transgenic plants some methods discussed. overexpression popular technique rice. study also offers several strategies using gene editing techniques. To feed world's rapidly expanding population, researchers improve food production.

Язык: Английский

Процитировано

10
Risk-appropriate, science-based innovation regulations are important DOI
Karinne Ludlow, José Benjamin Falck‐Zepeda, Stuart J. Smyth

и другие.

Trends in biotechnology, Год журнала: 2025, Номер 43(3), С. 502 - 510

Опубликована: Фев. 26, 2025

Язык: Английский

Процитировано

1
Recent Advances in Studying the Regulation of Fruit Ripening in Tomato Using Genetic Engineering Approaches DOI Open Access
Denis Baranov, Vadim Timerbaev

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(2), С. 760 - 760

Опубликована: Янв. 7, 2024

Tomato (Solanum lycopersicum L.) is one of the most commercially essential vegetable crops cultivated worldwide. In addition to nutritional value, tomato an excellent model for studying climacteric fruits’ ripening processes. Despite this, available natural pool genes that allows expanding phenotypic diversity limited, and difficulties crossing using classical selection methods when stacking traits increase proportionally with each additional feature. Modern genetic engineering tomatoes have extensive potential applications, such as enhancing expression existing gene(s), integrating artificial heterologous pointing changes in target gene sequences while keeping allelic combinations characteristic successful commercial varieties, many others. However, it necessary understand fundamental principles molecular regulation involved fruit its use creating new varieties. Although candidate mediate been identified, a complete picture their relationship has yet be formed. This review summarizes latest (2017–2023) achievements related processes fruits. work attempts systematize results various research articles display interaction pattern regulating process ripening.

Язык: Английский

Процитировано

6
Rethinking progress: harmonizing the discourse on genetically modified crops DOI Creative Commons
Abreham Bekele‐Alemu,

Obssi Dessalegn-Hora,

Tura Safawo-Jarso

и другие.

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Март 21, 2025

Genetically modified crops (GM crops) also known as biotech are that have been altered through genetic engineering techniques and under cultivation for approximately 28 years. By October 2024, over 30 nations approved the of GM crops. The global area utilized crop production has reached 206.3 million hectares. Despite substantial growth in these crops, debate continues between proponents opponents In this article, critical concerns common ground arguments both sides were described. main issues addressed include naturalness religious perspectives, beneficial aspects, safety issues, socio-economic impacts intellectual property rights. We argue classification unnatural is a claim lacks scientific reality. similar vein, comparing technology to act playing God inappropriate. Moreover, belief do not contribute yield improvements inconsistent with empirical evidence. Additionally, foods produced from unsafe human consumption holds unseen on ground. highlighted necessity implementing rights support seed developers limited duration without violating farmers’ conclusion, consumer right know what they eat, labeling food products fosters transparency enhance autonomy.

Язык: Английский

Процитировано

0
Is co-existence and/or containment of genetically modified plants possible, and is it important? DOI Creative Commons
J. R. Caradus

New Zealand Journal of Agricultural Research, Год журнала: 2025, Номер unknown, С. 1 - 52

Опубликована: Март 31, 2025

Язык: Английский

Процитировано

0
Microbiome engineering to palliate microbial dysbiosis occurring in agroecosystems DOI
Romain Darriaut, Céline Roose‐Amsaleg, Mathieu Vanhove

и другие.

Microbiological Research, Год журнала: 2025, Номер unknown, С. 128178 - 128178

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Processes for regulating genetically modified and gene edited plants DOI Creative Commons
J. R. Caradus

GM crops & food, Год журнала: 2023, Номер 14(1), С. 1 - 41

Опубликована: Сен. 10, 2023

Innovation in agriculture has been essential improving productivity of crops and forages to support a growing population, living standards while contributing toward maintaining environment integrity, human health, wellbeing through provision more nutritious, varied, abundant food sources. A crucial part that innovation involved range techniques for both expanding exploiting the genetic potential plants. However, some used generating new variation plant breeders exploit are deemed higher risk than others despite end products processes at times being all intents purposes identical benefits they provide. As result, public concerns often triggered by poor communication from innovators, resulting mistrust suspicion has, turn, caused development regulatory systems. The logic motivations modes regulation reviewed how use these technologies can be delivered efficiently effectively is discussed.

Язык: Английский

Процитировано

10
The current status of the use of genetic modification and editing to improve biodiversity and ecological sustainability DOI Creative Commons
Gideon Sadikiel Mmbando, Kelvin Ngongolo

All Life, Год журнала: 2024, Номер 17(1)

Опубликована: Окт. 25, 2024

Язык: Английский

Процитировано

3
Genetic Engineering in Bacteria, Fungi, and Oomycetes, Taking Advantage of CRISPR DOI Creative Commons

Piao Yang,

Abraham Condrich,

Ling Lü

и другие.

DNA, Год журнала: 2024, Номер 4(4), С. 427 - 454

Опубликована: Ноя. 14, 2024

Genetic engineering has revolutionized our ability to modify microorganisms for various applications in agriculture, medicine, and industry. This review examines recent advances genetic techniques bacteria, fungi, oomycetes, with a focus on CRISPR-Cas systems. In CRISPR-Cas9 enabled precise genome editing, enhancing antibiotic production metabolic engineering. For despite challenges associated their complex cell structures, CRISPR/Cas9 advanced the of enzymes secondary metabolites. significant plant pathogens, modified Agrobacterium-mediated transformation, CRISPR/Cas12a have contributed developing disease-resistant crops. provides comparative analysis efficiencies across these addresses ethical regulatory considerations. Future research directions include refining tools improve efficiency expand applicability non-model organisms. comprehensive overview highlights transformative potential microbiology its implications addressing global biotechnology.

Язык: Английский

Процитировано

3