Biomedicines,
Год журнала:
2025,
Номер
13(4), С. 858 - 858
Опубликована: Апрель 2, 2025
Background/Objectives:
Patients
with
central
nervous
system
injuries
who
are
hospitalized
in
intensive
care
units
(ICUs)
at
high
risk
for
nosocomial
infections.
Limited
data
available
on
the
incidence
and
patterns
of
microbial
colonization
infection
this
patient
population.
Methods:
To
fill
gap,
we
performed
an
electronic
health
record-based
study
1614
chronic
patients
brain
injury
admitted
to
ICU
from
2017
2023.
Results:
Among
infectious
complications,
pneumonia
was
most
common
(n
=
879;
54.46%).
Sepsis
diagnosed
54
patients,
whom
46
(85%)
were
pneumonia.
The
only
pathogen
that
showed
association
development
sepsis
colonized
Pseudomonas
aeruginosa
(pneumonia:
p
7.2
×
10−9;
sepsis:
1.7
10−5).
Bacterial
isolates
without
did
not
differ
titer
or
dynamics,
but
monomicrobial
culture
more
likely
develop
than
polymicrobial
(1
vs.
2
pathogens,
0.014;
1
+
pathogens
3
4
2.8
10−6),
although
lower
monoculture
polyculture.
all
sites
levels
multidrug
resistance
(Gram-negative
bacteria:
88–100%;
Gram-positive
48–97%),
no
differences
multidrug-resistant
organism
(MDRO)
rates.
Conclusions:
Our
results
highlight
burden
MDROs
neurological
ICUs
provide
novel
ecosystem-based
insights
into
mono-
development.
These
findings
may
be
useful
developing
strategies
protect
against
Frontiers in Cellular and Infection Microbiology,
Год журнала:
2023,
Номер
13
Опубликована: Апрель 6, 2023
Bacterial
biofilms
are
complex
microbial
communities
encased
in
extracellular
polymeric
substances.
Their
formation
is
a
multi-step
process.
Biofilms
significant
problem
treating
bacterial
infections
and
one
of
the
main
reasons
for
persistence
infections.
They
can
exhibit
increased
resistance
to
classical
antibiotics
cause
disease
through
device-related
non-device
(tissue)
-associated
infections,
posing
severe
threat
global
health
issues.
Therefore,
early
detection
search
new
alternative
treatments
essential
suppressing
biofilm-associated
In
this
paper,
we
systematically
reviewed
biofilms,
associated
methods,
potential
treatment
strategies,
aiming
provide
researchers
with
latest
progress
biofilms.
FEMS Microbiology Reviews,
Год журнала:
2023,
Номер
48(1)
Опубликована: Дек. 12, 2023
Rhizosphere
microbes
play
critical
roles
for
plant's
growth
and
health.
Among
them,
the
beneficial
rhizobacteria
have
potential
to
be
developed
as
biofertilizer
or
bioinoculants
sustaining
agricultural
development.
The
efficient
rhizosphere
colonization
of
these
is
a
prerequisite
exerting
their
plant
functions,
but
colonizing
process
underlying
mechanisms
not
been
thoroughly
reviewed,
especially
nonsymbiotic
rhizobacteria.
This
review
systematically
analyzed
root
compared
it
with
that
symbiotic
pathogenic
bacteria.
also
highlighted
approaches
improve
efficiency
proposed
study
rhizobacterial
from
holistic
perspective
microbiome
under
more
natural
conditions.
Antimicrobial Resistance and Infection Control,
Год журнала:
2023,
Номер
12(1)
Опубликована: Сен. 7, 2023
Biofilms
are
ubiquitous
in
healthcare
settings.
By
nature,
biofilms
less
susceptible
to
antimicrobials
and
associated
with
healthcare-associated
infections
(HAI).
Resistance
of
biofilm
is
multifactorial
the
presence
a
matrix
composed
extracellular
polymeric
substances
eDNA,
being
major
contributing
factor.
The
usual
multispecies
composition
environmental
can
also
impact
on
antimicrobial
efficacy.
In
settings,
two
main
types
present:
hydrated
biofilms,
for
example,
drains
parts
some
medical
devices
equipment,
dry
(DSB)
surfaces
possibly
devices.
act
as
reservoir
pathogens
including
multi-drug
resistant
organisms
their
elimination
requires
different
approaches.
control
(drain)
should
be
informed
by
reduction
or
microbial
bioburden
together
measuring
regrowth
time.
DSB
measured
combination
decrease
bacterial
transfer
post-intervention.
Failure
increases
risk
HAI,
but
not
solely
responsible
disinfection
failure
shortcoming.
limited
number
standardised
efficacy
tests
hindrance
end
users
manufacturers,
whilst
Europe
there
no
approved
standard
protocols.
Education
stakeholders
about
ad
hoc
tests,
often
academic
thus
paramount,
achieve
better
Comprehensive Reviews in Food Science and Food Safety,
Год журнала:
2022,
Номер
21(4), С. 3346 - 3375
Опубликована: Июнь 28, 2022
Abstract
Food
fermentation
is
driven
by
microorganisms,
which
usually
coexist
as
multispecies
biofilms.
The
activities
and
interactions
of
functional
microorganisms
pathogenic
bacteria
in
biofilms
have
important
implications
for
the
quality
safety
fermented
foods.
It
was
verified
that
biofilm
lifestyle
benefited
fitness
harsh
environments
intensified
cooperation
competition
between
members.
This
review
focuses
on
formation,
microbial
communication
biofilms,
application
food
fermentation.
Microbial
aggregation
adhesion
are
steps
early
stage
formation.
Different
biofilm‐forming
abilities
strategies
among
lead
to
several
types
spatial
distribution
reflects
function.
Then,
we
discuss
intrinsic
factors
external
manifestations
system
succession.
Several
typical
interspecies
modes
mechanisms
were
reviewed
this
review.
main
limitations
studies
included
relatively
small
number
formed
during
lack
direct
evidence
formation
process
within
aims
provide
industry
with
a
sufficient
understanding
Practical
Application
:
Meanwhile,
it
offers
reference
value
better
controlling
utilizing
process,
improvement
yield,
quality,
products
including
Chinese
Baijiu
,
cheeese,
kefir
soy
sauce,
kombucha,
olive.
The ISME Journal,
Год журнала:
2023,
Номер
17(6), С. 803 - 812
Опубликована: Март 4, 2023
Abstract
Extracellular
polymeric
substances
(EPS)
are
core
biofilm
components,
yet
how
they
mediate
interactions
within
and
contribute
to
the
structuring
of
biofilms
is
largely
unknown,
particularly
for
non-culturable
microbial
communities
that
predominate
in
environmental
habitats.
To
address
this
knowledge
gap,
we
explored
role
EPS
an
anaerobic
ammonium
oxidation
(anammox)
biofilm.
An
extracellular
glycoprotein,
BROSI_A1236,
from
anammox
bacterium,
formed
envelopes
around
cells,
supporting
its
identification
as
a
surface
(S-)
layer
protein.
However,
S-layer
protein
also
appeared
at
edge
biofilm,
close
proximity
polysaccharide-coated
filamentous
Chloroflexi
bacteria
but
distal
bacterial
cells.
The
assembled
into
cross-linked
network
granules
surrounding
cell
clusters,
with
occupying
space
Chloroflexi.
was
abundant
junctions
between
Thus,
likely
transported
through
matrix
acts
adhesive
facilitate
assembly
three-dimensional
lattice.
spatial
distribution
mixed
species
suggests
it
“public-good”
EPS,
which
facilitates
other
framework
benefit
community,
enables
key
syntrophic
relationships,
including
anammox.
Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Май 22, 2023
Abstract
Streptococcus
mutans
has
been
implicated
as
the
primary
pathogen
in
childhood
caries
(tooth
decay).
While
role
of
polymicrobial
communities
is
appreciated,
it
remains
unclear
whether
other
microorganisms
are
active
contributors
or
interact
with
pathogens.
Here,
we
integrate
multi-omics
supragingival
biofilm
(dental
plaque)
from
416
preschool-age
children
(208
males
and
208
females)
a
discovery-validation
pipeline
to
identify
disease-relevant
inter-species
interactions.
Sixteen
taxa
associate
metagenomics-metatranscriptomics
analyses.
Using
multiscale/computational
imaging
virulence
assays,
examine
formation
dynamics,
spatial
arrangement,
metabolic
activity
Selenomonas
sputigena,
Prevotella
salivae
Leptotrichia
wadei
,
either
individually
S.
.
We
show
that
sputigena
flagellated
anaerobe
previously
unknown
biofilm,
becomes
trapped
streptococcal
exoglucans,
loses
motility
but
actively
proliferates
build
honeycomb-like
multicellular-superstructure
encapsulating
enhancing
acidogenesis.
Rodent
model
experiments
reveal
an
unrecognized
ability
colonize
tooth
surfaces.
incapable
causing
on
its
own,
when
co-infected
mutans,
causes
extensive
enamel
lesions
exacerbates
disease
severity
vivo.
In
summary,
discover
pathobiont
cooperating
known
unique
structure
heighten
prevalent
human
disease.
FEMS Microbiology Reviews,
Год журнала:
2024,
Номер
48(2)
Опубликована: Фев. 28, 2024
Abstract
Microbiomes
are
foundational
components
of
the
environment
that
provide
essential
services
relating
to
food
security,
carbon
sequestration,
human
health,
and
overall
well-being
ecosystems.
Microbiota
exert
their
effects
primarily
through
complex
interactions
at
interfaces
with
plant,
animal,
hosts,
as
well
within
soil
environment.
This
review
aims
explore
ecological,
evolutionary,
molecular
processes
governing
establishment
function
microbiome–host
relationships,
specifically
critical
One
Health—a
transdisciplinary
framework
recognizes
health
outcomes
people,
animals,
plants,
tightly
interconnected.
Within
context
Health,
core
principles
underpinning
microbiome
assembly
will
be
discussed
in
detail,
including
biofilm
formation,
microbial
recruitment
strategies,
mechanisms
attachment,
community
succession,
effect
these
have
on
host
health.
Finally,
this
catalogue
recent
advances
microbiology
ecology
methods
can
used
profile
interfaces,
particular
attention
multi-omic,
advanced
imaging,
modelling
approaches.
These
technologies
for
delineating
general
specific
functions,
mapping
interconnectivity
across
varying
spatial
temporal
scales,
predictive
frameworks
guide
development
targeted
microbiome-interventions
deliver
Health
outcomes.
Frontiers in Microbiology,
Год журнала:
2023,
Номер
14
Опубликована: Апрель 13, 2023
Most
biofilms
within
the
food
industry
are
formed
by
multiple
bacterial
species
which
co-exist
on
surfaces
as
a
result
of
interspecies
interactions.
These
ecological
interactions
often
make
these
communities
tolerant
against
antimicrobials.
Our
previous
work
led
to
identification
large
number
(327)
highly
diverse
contact
dairy,
meat,
and
egg
industries
after
routine
cleaning
disinfection
(C&D)
regimes.
In
current
study,
biofilm-forming
ability
92
strains
belonging
26
genera
42
was
assessed
synergistic
in
biofilm
formation
were
investigated
coculturing
all
possible
four-species
combinations.
Out
total
455
combinations,
greater
mass
production,
compared
sum
masses
individual
monoculture,
observed
34
Around
half
combinations
showed
synergy
>
1.5-fold
most
belonged
dairy
strains.
The
highest
(3.13-fold)
shown
combination
comprising
Stenotrophomonas
rhizophila,
Bacillus
licheniformis,
Microbacterium
lacticum,
Calidifontibacter
indicus.
mixed
turned
out
be
strain-specific
rather
than
species-dependent.
All
showing
remarkable
appeared
have
certain
common
shows
there
keystone
industry-specific
stimulate
or
antagonism
this
may
implication
for
control
concerned
industries.
