Observational Study of Microbial Colonization and Infection in Neurological Intensive Care Patients Based on Electronic Health Records DOI Creative Commons
А. С. Грачева, А. Н. Кузовлев, Lyubov E. Salnikova

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(4), P. 858 - 858

Published: April 2, 2025

Background/Objectives: Patients with central nervous system injuries who are hospitalized in intensive care units (ICUs) at high risk for nosocomial infections. Limited data available on the incidence and patterns of microbial colonization infection this patient population. Methods: To fill gap, we performed an electronic health record-based study 1614 chronic patients brain injury admitted to ICU from 2017 2023. Results: Among infectious complications, pneumonia was most common (n = 879; 54.46%). Sepsis diagnosed 54 patients, whom 46 (85%) were pneumonia. The only pathogen that showed association development sepsis colonized Pseudomonas aeruginosa (pneumonia: p 7.2 × 10−9; sepsis: 1.7 10−5). Bacterial isolates without did not differ titer or dynamics, but monomicrobial culture more likely develop than polymicrobial (1 vs. 2 pathogens, 0.014; 1 + pathogens 3 4 2.8 10−6), although lower monoculture polyculture. all sites levels multidrug resistance (Gram-negative bacteria: 88–100%; Gram-positive 48–97%), no differences multidrug-resistant organism (MDRO) rates. Conclusions: Our results highlight burden MDROs neurological ICUs provide novel ecosystem-based insights into mono- development. These findings may be useful developing strategies protect against

Language: Английский

Understanding bacterial biofilms: From definition to treatment strategies DOI Creative Commons
Ailing Zhao,

Jiazheng Sun,

Yipin Liu

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2023, Volume and Issue: 13

Published: April 6, 2023

Bacterial biofilms are complex microbial communities encased in extracellular polymeric substances. Their formation is a multi-step process. Biofilms significant problem treating bacterial infections and one of the main reasons for persistence infections. They can exhibit increased resistance to classical antibiotics cause disease through device-related non-device (tissue) -associated infections, posing severe threat global health issues. Therefore, early detection search new alternative treatments essential suppressing biofilm-associated In this paper, we systematically reviewed biofilms, associated methods, potential treatment strategies, aiming provide researchers with latest progress biofilms.

Language: Английский

Citations

207
Root colonization by beneficial rhizobacteria DOI Creative Commons
Yunpeng Liu, Zhihui Xu, Lin Chen

et al.

FEMS Microbiology Reviews, Journal Year: 2023, Volume and Issue: 48(1)

Published: Dec. 12, 2023

Rhizosphere microbes play critical roles for plant's growth and health. Among them, the beneficial rhizobacteria have potential to be developed as biofertilizer or bioinoculants sustaining agricultural development. The efficient rhizosphere colonization of these is a prerequisite exerting their plant functions, but colonizing process underlying mechanisms not been thoroughly reviewed, especially nonsymbiotic rhizobacteria. This review systematically analyzed root compared it with that symbiotic pathogenic bacteria. also highlighted approaches improve efficiency proposed study rhizobacterial from holistic perspective microbiome under more natural conditions.

Language: Английский

Citations

49
How biofilm changes our understanding of cleaning and disinfection DOI Creative Commons
Jean‐Yves Maillard, Isabella Centeleghe

Antimicrobial Resistance and Infection Control, Journal Year: 2023, Volume and Issue: 12(1)

Published: Sept. 7, 2023

Biofilms are ubiquitous in healthcare settings. By nature, biofilms less susceptible to antimicrobials and associated with healthcare-associated infections (HAI). Resistance of biofilm is multifactorial the presence a matrix composed extracellular polymeric substances eDNA, being major contributing factor. The usual multispecies composition environmental can also impact on antimicrobial efficacy. In settings, two main types present: hydrated biofilms, for example, drains parts some medical devices equipment, dry (DSB) surfaces possibly devices. act as reservoir pathogens including multi-drug resistant organisms their elimination requires different approaches. control (drain) should be informed by reduction or microbial bioburden together measuring regrowth time. DSB measured combination decrease bacterial transfer post-intervention. Failure increases risk HAI, but not solely responsible disinfection failure shortcoming. limited number standardised efficacy tests hindrance end users manufacturers, whilst Europe there no approved standard protocols. Education stakeholders about ad hoc tests, often academic thus paramount, achieve better

Language: Английский

Citations

46
Population genetics, biofilm recalcitrance, and antibiotic resistance evolution DOI
Barbora Trubenová, Dan Roizman, Annette Moter

et al.

Trends in Microbiology, Journal Year: 2022, Volume and Issue: 30(9), P. 841 - 852

Published: March 23, 2022

Language: Английский

Citations

59
Multispecies biofilms in fermentation: Biofilm formation, microbial interactions, and communication DOI

Shangjie Yao,

Liying Hao,

Rongqing Zhou

et al.

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2022, Volume and Issue: 21(4), P. 3346 - 3375

Published: June 28, 2022

Abstract Food fermentation is driven by microorganisms, which usually coexist as multispecies biofilms. The activities and interactions of functional microorganisms pathogenic bacteria in biofilms have important implications for the quality safety fermented foods. It was verified that biofilm lifestyle benefited fitness harsh environments intensified cooperation competition between members. This review focuses on formation, microbial communication biofilms, application food fermentation. Microbial aggregation adhesion are steps early stage formation. Different biofilm‐forming abilities strategies among lead to several types spatial distribution reflects function. Then, we discuss intrinsic factors external manifestations system succession. Several typical interspecies modes mechanisms were reviewed this review. main limitations studies included relatively small number formed during lack direct evidence formation process within aims provide industry with a sufficient understanding Practical Application : Meanwhile, it offers reference value better controlling utilizing process, improvement yield, quality, products including Chinese Baijiu , cheeese, kefir soy sauce, kombucha, olive.

Language: Английский

Citations

51
Surface-layer protein is a public-good matrix exopolymer for microbial community organisation in environmental anammox biofilms DOI Creative Commons
Lan Li Wong, Yang Lü, James C. S. Ho

et al.

The ISME Journal, Journal Year: 2023, Volume and Issue: 17(6), P. 803 - 812

Published: March 4, 2023

Abstract Extracellular polymeric substances (EPS) are core biofilm components, yet how they mediate interactions within and contribute to the structuring of biofilms is largely unknown, particularly for non-culturable microbial communities that predominate in environmental habitats. To address this knowledge gap, we explored role EPS an anaerobic ammonium oxidation (anammox) biofilm. An extracellular glycoprotein, BROSI_A1236, from anammox bacterium, formed envelopes around cells, supporting its identification as a surface (S-) layer protein. However, S-layer protein also appeared at edge biofilm, close proximity polysaccharide-coated filamentous Chloroflexi bacteria but distal bacterial cells. The assembled into cross-linked network granules surrounding cell clusters, with occupying space Chloroflexi. was abundant junctions between Thus, likely transported through matrix acts adhesive facilitate assembly three-dimensional lattice. spatial distribution mixed species suggests it “public-good” EPS, which facilitates other framework benefit community, enables key syntrophic relationships, including anammox.

Language: Английский

Citations

37
Selenomonas sputigena acts as a pathobiont mediating spatial structure and biofilm virulence in early childhood caries DOI Creative Commons
Hunyong Cho, Zhi Ren, Kimon Divaris

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: May 22, 2023

Abstract Streptococcus mutans has been implicated as the primary pathogen in childhood caries (tooth decay). While role of polymicrobial communities is appreciated, it remains unclear whether other microorganisms are active contributors or interact with pathogens. Here, we integrate multi-omics supragingival biofilm (dental plaque) from 416 preschool-age children (208 males and 208 females) a discovery-validation pipeline to identify disease-relevant inter-species interactions. Sixteen taxa associate metagenomics-metatranscriptomics analyses. Using multiscale/computational imaging virulence assays, examine formation dynamics, spatial arrangement, metabolic activity Selenomonas sputigena, Prevotella salivae Leptotrichia wadei , either individually S. . We show that sputigena flagellated anaerobe previously unknown biofilm, becomes trapped streptococcal exoglucans, loses motility but actively proliferates build honeycomb-like multicellular-superstructure encapsulating enhancing acidogenesis. Rodent model experiments reveal an unrecognized ability colonize tooth surfaces. incapable causing on its own, when co-infected mutans, causes extensive enamel lesions exacerbates disease severity vivo. In summary, discover pathobiont cooperating known unique structure heighten prevalent human disease.

Language: Английский

Citations

26
Life at the borderlands: microbiomes of interfaces critical to One Health DOI Creative Commons
Simon R. Law, Falko Mathes, Amy M. Paten

et al.

FEMS Microbiology Reviews, Journal Year: 2024, Volume and Issue: 48(2)

Published: Feb. 28, 2024

Abstract Microbiomes are foundational components of the environment that provide essential services relating to food security, carbon sequestration, human health, and overall well-being ecosystems. Microbiota exert their effects primarily through complex interactions at interfaces with plant, animal, hosts, as well within soil environment. This review aims explore ecological, evolutionary, molecular processes governing establishment function microbiome–host relationships, specifically critical One Health—a transdisciplinary framework recognizes health outcomes people, animals, plants, tightly interconnected. Within context Health, core principles underpinning microbiome assembly will be discussed in detail, including biofilm formation, microbial recruitment strategies, mechanisms attachment, community succession, effect these have on host health. Finally, this catalogue recent advances microbiology ecology methods can used profile interfaces, particular attention multi-omic, advanced imaging, modelling approaches. These technologies for delineating general specific functions, mapping interconnectivity across varying spatial temporal scales, predictive frameworks guide development targeted microbiome-interventions deliver Health outcomes.

Language: Английский

Citations

9
Synergistic interactions in multispecies biofilm combinations of bacterial isolates recovered from diverse food processing industries DOI Creative Commons
Faizan Ahmed Sadiq, Koen De Reu, Mette Burmølle

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: April 13, 2023

Most biofilms within the food industry are formed by multiple bacterial species which co-exist on surfaces as a result of interspecies interactions. These ecological interactions often make these communities tolerant against antimicrobials. Our previous work led to identification large number (327) highly diverse contact dairy, meat, and egg industries after routine cleaning disinfection (C&D) regimes. In current study, biofilm-forming ability 92 strains belonging 26 genera 42 was assessed synergistic in biofilm formation were investigated coculturing all possible four-species combinations. Out total 455 combinations, greater mass production, compared sum masses individual monoculture, observed 34 Around half combinations showed synergy > 1.5-fold most belonged dairy strains. The highest (3.13-fold) shown combination comprising Stenotrophomonas rhizophila, Bacillus licheniformis, Microbacterium lacticum, Calidifontibacter indicus. mixed turned out be strain-specific rather than species-dependent. All showing remarkable appeared have certain common shows there keystone industry-specific stimulate or antagonism this may implication for control concerned industries.

Language: Английский

Citations

21
Phytoremediation: Harnessing plant power and innovative technologies for effective soil remediation DOI Creative Commons
Malika Oubohssaine,

Ikram Dahmani

Plant Stress, Journal Year: 2024, Volume and Issue: 14, P. 100578 - 100578

Published: Aug. 27, 2024

Language: Английский

Citations

7