Advances in RNA Interference for Plant Functional Genomics: Unveiling Traits, Mechanisms, and Future Directions

Applied Biochemistry and Biotechnology, Год журнала: 2024, Номер 196(9), С. 5681 - 5710

Опубликована: Янв. 4, 2024

Язык: Английский

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Challenges and Opportunities Arising from Host–Botrytis cinerea Interactions to Outline Novel and Sustainable Control Strategies: The Key Role of RNA Interference

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(12), С. 6798 - 6798

Опубликована: Июнь 20, 2024

The necrotrophic plant pathogenic fungus Botrytis cinerea (Pers., 1794), the causative agent of gray mold disease, causes significant losses in agricultural production. Control this fungal pathogen is quite difficult due to its wide host range and environmental persistence. Currently, management disease still mainly based on chemicals, which can have harmful effects not only environment human health but also because they favor development strains resistant fungicides. flexibility plasticity B. challenging defense mechanisms ability evolve strategies escape chemicals require new control for successful management. In review, some aspects host-pathogen interactions from novel sustainable could be developed (e.g., signaling pathways, molecules involved immune mechanisms, hormones, post-transcriptional gene silencing) were analyzed. New biotechnological tools use RNA interference (RNAi) are emerging crop protection scenario as versatile, sustainable, effective, environmentally friendly alternatives chemicals. RNAi-based fungicides expected approved soon, although will face several challenges before reaching market.

Язык: Английский

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Neuroprotection and Mechanism of Gas-miR36-5p from Gastrodia elata in an Alzheimer’s Disease Model by Regulating Glycogen Synthase Kinase-3β

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(24), С. 17295 - 17295

Опубликована: Дек. 9, 2023

Alzheimer’s disease (AD) is currently the most common neurodegenerative disease. Glycogen synthase kinase 3β (GSK-3β) a pivotal factor in AD pathogenesis. Recent research has demonstrated that plant miRNAs exert cross-kingdom regulation on target genes animals. Gastrodia elata (G. elata) valuable traditional Chinese medicine significant pharmacological activity against diseases of central nervous system (CNS). Our previous studies have indicated G. elata-specific miRNA plays regulatory role for NF-κB signaling pathway mice. In this study, further bioinformatics analysis suggested Gas-miR36-5p targets GSK-3β. Through western blot, RT-qPCR, and assessments T-AOC, SOD, MDA levels, its neuroprotective effects an cell model. Furthermore, was detected murine brain tissues. The results Morris water maze test blot provided positive evidence reversing learning deficits hyperphosphorylation Tau mice, elucidating model following RNA administration. emphasizes as novel with properties by targeting Consequently, our findings provide insights into mechanisms underlying miRNA, presenting perspective treatment

Язык: Английский

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Dietary Epigenetic Modulators: Unravelling the Still-Controversial Benefits of miRNAs in Nutrition and Disease

Nutrients, Год журнала: 2024, Номер 16(1), С. 160 - 160

Опубликована: Янв. 3, 2024

In the context of nutrient-driven epigenetic alterations, food-derived miRNAs can be absorbed into circulatory system and organs recipients, especially humans, potentially contribute to modulating health diseases. Evidence suggests that food uptake, by carrying exogenous (xenomiRNAs), regulates individual miRNA profile, modifying redox homeostasis inflammatory conditions underlying pathological processes, such as type 2 diabetes mellitus, insulin resistance, metabolic syndrome, cancer. The capacity diet control levels comprehension unique characteristics dietary in terms gene expression regulation show important perspectives a strategy disease susceptibility via modifications refine clinical outcomes. However, absorption, stability, availability, roles are intriguing currently subject intense debate; additionally, there is restricted knowledge their physiological potential side effects. Within this framework, we provided up-to-date comprehensive on miRNAs’ potential, discussing latest advances controversial issues related role human modulators chronic syndromes.

Язык: Английский

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MicroRNA tools for gene silencing

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 215 - 232

Опубликована: Янв. 1, 2025

Язык: Английский

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Impact of Nutrient Stress on Plant Disease Resistance

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1780 - 1780

Опубликована: Фев. 19, 2025

Plants are constantly exposed to abiotic and biotic stresses that seriously affect crop yield quality. A coordinated regulation of plant responses combined abiotic/biotic requires crosstalk between signaling pathways initiated by each stressor. Interconnected further finetune stress allow the respond such effectively. The nutritional status might influence disease resistance strengthening or weakening immune responses, as well through modulation pathogenicity program in pathogen. Here, we discuss advances our understanding interactions nutrient stress, deficiency excess, context current agricultural practices. introduction chemical fertilizers pesticides was a major component Green Revolution 1960s greatly boosted production. However, massive application agrochemicals also has adverse consequences on environment animal/human health. Therefore, an in-depth connections caused overfertilization (or low bioavailability nutrients) is timely novel field research with important implications for control species. Optimizing management practices tailored specific environmental conditions will be crucial maximizing production using environmentally friendly systems.

Язык: Английский

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Gene silencing by RNA interference: a review

Genome Instability & Disease, Год журнала: 2024, Номер 5(5), С. 225 - 241

Опубликована: Авг. 9, 2024

Язык: Английский

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Identification of miRNAs Involving Potato-Phytophthora infestans Interaction

Plants, Год журнала: 2023, Номер 12(3), С. 461 - 461

Опубликована: Янв. 19, 2023

sRNAs (small RNAs) play an important role in regulation of plant immunity against a variety pathogens. In this study, sRNA sequencing analysis was performed to identify miRNAs (microRNAs) during the interaction potato and Phytophthora infestans. Totally, 171 were identified, 43 which annotated miRNA database 128 assigned as novel study. Those may target 878 genes half them encode resistance proteins. Fifty-three 194 P. infestans genes. Three (novel 72, 133, 140) predicted have targets only genome. transient expression inoculation assay showed that miR396, miR166, miR6149-5P, novel133, or novel140 promoted colonization, while miR394 inhibited colonization on Nicotiana benthamiana leaves. An artificial (amiRNA) degradation experiment demonstrated could both gene (PGSC0003DMG400034305) miR396 multicystatin (PGSC0003DMG400026899) miR6149-5p shear galactose oxidase F-box protein CPR30 (PGSC0003DMG400021641). This study provides new information aspect cross-kingdom immune potato-P. at level.

Язык: Английский

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In-depth investigation of microRNA-mediated cross-kingdom regulation between Asian honey bee and microsporidian

Frontiers in Microbiology, Год журнала: 2022, Номер 13

Опубликована: Сен. 29, 2022

Asian honey bee Apis cerana is the original host for Nosema ceranae, a unicellular fungal parasite that causes nosemosis throughout world. Currently, interaction between A. and N. ceranae largely unknown. Our group previously prepared c. workers' midguts at 7 days post inoculation (dpi) 10 dpi with spores as well corresponding un-inoculated midguts, followed by cDNA library construction combination of RNAs-seq small RNA-seq. Meanwhile, we clean which were then subjected to deep sequencing. Here, based on gained high-quality transcriptome datasets, differentially expressed mRNAs (DEmiRNAs) targeted DEmiRNAs, DEmRNAs microsporidian DEmiRNAs deeply investigated, focus targets involved in glycolysis/glyconeogenesis virulence factors, energy metabolism immune response. In worker's (10) (days inoculation), eight (seven) up-regulated six (two) down-regulated miRNAs observed target 97 (44) 60 (15) mRNAs, respectively. Additionally, two (miR-60-y miR-676-y) midgut could genes engaged spore wall protein glycolysis/gluconeogenesis, indicating potential miRNA-mediated regulation factor metabolism. dpi, 121 (110) 112 (104) found to, respectively, 343 (247) 138 midguts. These relevant several crucial cellular humoral pathways, such phagasome, endocytosis, lysosomes, autophagy, Jak-STAT signaling pathway, indicative involvement regulating these pathways. addition, miR-21-x was had relative oxidative phosphorylation, suggesting may be used weapon modulate this pivotal pathway. Furthermore, targeting relationships pairs DEmiRNAs-microsporidian DEmiRNAs-host validated using RT-qPCR. findings not only lay foundation exploring molecular mechanism underlying cross-kingdom workers but also offer valuable insights into bee-microsporidian interaction.

Язык: Английский

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Exploring the putative microRNAs cross-kingdom transfer in Solanum lycopersicum-Meloidogyne incognita interactions bioinformatics and in vitro assays

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Май 8, 2024

Introduction Plant-pathogen interaction is an inexhaustible source of information on how to sustainably control diseases that negatively affect agricultural production. Meloidogyne incognita a root-knot nematode (RKN), representing pest for many crops, including tomato ( Solanum lycopersicum ). RKNs are global threat agriculture, especially under climate change, and RNA technologies offer potential alternative chemical nematicides. While endogenous microRNAs have been identified in both S. M. , their roles related the regulation developmental changes, no study has investigated miRNAs cross-kingdom transfer during this interaction. Methods Here, we propose bioinformatics pipeline highlight miRNA-dependent interactions between . Results The obtained data show putatively targeting genes mostly detrimental effects plant development defense. Similarly, biological processes negative digestion, mobility, reproduction. To experimentally test hypothesis, vitro feeding assay was carried out using sly-miRNAs selected from approach. results two (sly-miRNA156a, sly-miR169f) soaked by juvenile larvae (J2s) affected ability infect roots form galls. This also coupled with significant downregulation predicted target Minc11367, Minc00111 ), as revealed qRT-PCR analysis. Discussions Therefore, current expands knowledge involvement host-parasite could pave way application exogenous tools infection.

Язык: Английский

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