Frontiers in Plant Science,
Год журнала:
2023,
Номер
14
Опубликована: Окт. 9, 2023
CRISPR-Cas9,
the
“genetic
scissors”,
is
being
presaged
as
a
revolutionary
technology,
having
tremendous
potential
to
create
designer
crops
by
introducing
precise
and
targeted
modifications
in
genome
achieve
global
food
security
face
of
climate
change
increasing
population.
Traditional
genetic
engineering
relies
on
random
unpredictable
insertion
isolated
genes
or
foreign
DNA
elements
into
plant
genome.
However,
CRISPR-Cas
based
gene
editing
does
not
necessarily
involve
inserting
element
from
different
species
but
new
traits
precisely
altering
existing
genes.
CRISPR
edited
are
touching
markets,
however,
world
community
divided
over
whether
these
should
be
considered
genetically
modified
(GM)
non-GM.
Classification
crops,
especially
transgene
free
traditional
GM
will
significantly
affect
their
future
public
acceptance
some
regions.
Therefore,
depending
upon
regulation
non-GMs,
perception.
Here
we
briefly
discuss
how
crops.
In
addition,
reagents
delivery
tools
produce
transgene-free
Moreover,
also
summarize
regulatory
classification
countries
regulating
We
that
controversy
CRISPR-edited
plants
non-GM
continue
until
universal,
transparent,
scalable
framework
for
introduced
worldwide,
with
increased
awareness
involving
all
stakeholders.
Frontiers in Plant Science,
Год журнала:
2022,
Номер
13
Опубликована: Май 18, 2022
The
development
of
plant
varieties
with
desired
traits
is
imperative
to
ensure
future
food
security.
revolution
genome
editing
technologies
based
on
the
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)/CRISPR-associated
nuclease
9
(Cas9)
system
has
ushered
in
a
new
era
breeding.
Cas9
and
single-guide
RNA
(sgRNA)
form
an
effective
targeting
complex
locus
or
loci
interest,
enabling
all
plants
high
accuracy
efficiency.
Therefore,
CRISPR/Cas9
can
save
both
time
labor
relative
what
typically
associated
traditional
breeding
methods.
However,
despite
improvements
gene
editing,
several
challenges
remain
that
limit
application
CRISPR/Cas9-based
plants.
Here,
we
focus
four
issues
relevant
editing:
(1)
organelle
editing;
(2)
transgene-free
(3)
virus-induced
(4)
recalcitrant
elite
crop
inbred
lines.
This
review
provides
up-to-date
summary
state
CRISPR/Cas9-mediated
will
push
this
technique
forward.
Polymers,
Год журнала:
2022,
Номер
14(11), С. 2287 - 2287
Опубликована: Июнь 4, 2022
Nanotechnology
plays
an
important
role
in
biological
research,
especially
the
development
of
delivery
systems
with
lower
toxicity
and
greater
efficiency.
These
include
not
only
metallic
nanoparticles,
but
also
biopolymeric
nanoparticles.
Biopolymeric
nanoparticles
(BPNs)
are
mainly
developed
for
their
provision
several
advantages,
such
as
biocompatibility,
biodegradability,
minimal
toxicity,
addition
to
general
advantages
Therefore,
given
that
biopolymers
biodegradable,
natural,
environmentally
friendly,
they
have
attracted
great
attention
due
multiple
applications
biomedicine,
drug
delivery,
antibacterial
activity,
etc.
This
review
on
highlights
various
synthesis
methods,
ionic
gelation
method,
nanoprecipitation
microemulsion
method.
In
addition,
covers
biodegradable
polymeric
different
areas-especially
pharmaceutical,
biomedical,
agricultural
domains.
conclusion,
present
recent
advances
presents
both
fundamental
applied
aspects
can
be
used
further
field
Frontiers in Plant Science,
Год журнала:
2023,
Номер
14
Опубликована: Фев. 7, 2023
The
acceptance
of
new
crop
varieties
by
consumers
is
contingent
on
the
presence
consumer-preferred
traits,
which
include
sensory
attributes,
nutritional
value,
industrial
products
and
bioactive
compounds
production.
Recent
developments
in
genome
editing
technologies
provide
novel
insight
to
identify
gene
functions
improve
various
qualitative
quantitative
traits
commercial
importance
plants.
Various
conventional
as
well
advanced
gene-mutagenesis
techniques
such
physical
chemical
mutagenesis,
CRISPR-Cas9,
Cas12
base
editors
are
used
for
trait
improvement
crops.
To
meet
consumer
demand,
breakthrough
biotechnologies,
especially
CRISPR-Cas
have
received
a
fair
share
scientific
interest,
particularly
plant
editing.
versatile
tool
that
can
be
knock
out,
replace
knock-in
desired
fragments
at
targeted
locations
genome,
resulting
heritable
mutations
interest.
This
review
highlights
existing
literature
recent
(base
editing,
prime
multiplex
epigenome
delivery
methods)
reliable
precise
also
discusses
potential
exhibited
crops
consumer-demanded
higher
colour,
texture,
aroma/flavour,
production
biofuel,
fibre,
rubber
pharmaceuticals.
In
addition,
bottlenecks
challenges
associated
with
system,
off
targeting,
ploidy
level
ability
edit
organelle
been
discussed.
Frontiers in Plant Science,
Год журнала:
2023,
Номер
14
Опубликована: Окт. 9, 2023
CRISPR-Cas9,
the
“genetic
scissors”,
is
being
presaged
as
a
revolutionary
technology,
having
tremendous
potential
to
create
designer
crops
by
introducing
precise
and
targeted
modifications
in
genome
achieve
global
food
security
face
of
climate
change
increasing
population.
Traditional
genetic
engineering
relies
on
random
unpredictable
insertion
isolated
genes
or
foreign
DNA
elements
into
plant
genome.
However,
CRISPR-Cas
based
gene
editing
does
not
necessarily
involve
inserting
element
from
different
species
but
new
traits
precisely
altering
existing
genes.
CRISPR
edited
are
touching
markets,
however,
world
community
divided
over
whether
these
should
be
considered
genetically
modified
(GM)
non-GM.
Classification
crops,
especially
transgene
free
traditional
GM
will
significantly
affect
their
future
public
acceptance
some
regions.
Therefore,
depending
upon
regulation
non-GMs,
perception.
Here
we
briefly
discuss
how
crops.
In
addition,
reagents
delivery
tools
produce
transgene-free
Moreover,
also
summarize
regulatory
classification
countries
regulating
We
that
controversy
CRISPR-edited
plants
non-GM
continue
until
universal,
transparent,
scalable
framework
for
introduced
worldwide,
with
increased
awareness
involving
all
stakeholders.
