The Journal of Cell Biology,
Год журнала:
2022,
Номер
221(5)
Опубликована: Март 16, 2022
Very
little
is
known
about
how
the
material
properties
of
protein
condensates
assembled
via
liquid–liquid
phase
separation
(LLPS)
are
maintained
and
affect
physiological
functions.
Here
we
show
that
liquid-like
transcription
factor
TFEB
exhibit
low
fusion
propensity
in
vitro
living
cells.
We
directly
measured
attraction
force
between
droplets,
characterized
interfacial
tension,
viscosity,
elasticity
condensates.
contain
rigid
boundaries
govern
their
interaction
behaviors.
Several
small
molecules,
including
Ro-3306,
modify
condensates,
increasing
size
propensity.
These
compounds
promote
lysosomal
biogenesis
function
a
TFEB-dependent
manner
without
changing
its
cytoplasmic-nuclear
translocation.
Ro-3306
promotes
autophagy
activity,
facilitating
degradation
toxic
aggregates.
Our
study
helps
explain
as
physically
separate
entities
reveals
can
be
harnessed
to
modulate
activity.
Molecular Metabolism,
Год журнала:
2023,
Номер
74, С. 101755 - 101755
Опубликована: Июнь 16, 2023
Recently,
the
hallmarks
of
aging
were
updated
to
include
dysbiosis,
disabled
macroautophagy,
and
chronic
inflammation.
In
particular,
low-grade
inflammation
during
aging,
without
overt
infection,
is
defined
as
"inflammaging,"
which
associated
with
increased
morbidity
mortality
in
population.
Emerging
evidence
suggests
a
bidirectional
cyclical
relationship
between
development
age-related
conditions,
such
cardiovascular
diseases,
neurodegeneration,
cancer,
frailty.
How
crosstalk
other
underlies
biological
mechanisms
disease
thus
particular
interest
current
geroscience
research.
Signal Transduction and Targeted Therapy,
Год журнала:
2022,
Номер
7(1)
Опубликована: Июль 8, 2022
Abstract
Liquid–liquid
phase
separation
(LLPS)
is
a
novel
principle
for
explaining
the
precise
spatial
and
temporal
regulation
in
living
cells.
LLPS
compartmentalizes
proteins
nucleic
acids
into
micron-scale,
liquid-like,
membraneless
bodies
with
specific
functions,
which
were
recently
termed
biomolecular
condensates.
Biomolecular
condensates
are
executors
underlying
intracellular
spatiotemporal
coordination
of
various
biological
activities,
including
chromatin
organization,
genomic
stability,
DNA
damage
response
repair,
transcription,
signal
transduction.
Dysregulation
these
cellular
processes
key
event
initiation
and/or
evolution
cancer,
emerging
evidence
has
linked
formation
to
malignant
transformations
tumor
biology.
In
this
review,
we
comprehensively
summarize
detailed
mechanisms
condensate
biophysical
function
review
recent
major
advances
toward
elucidating
multiple
involved
cancer
cell
pathology
driven
by
aberrant
LLPS.
addition,
discuss
therapeutic
perspectives
research
most
developed
drug
candidates
targeting
modulation
that
can
be
used
combat
tumorigenesis.
Autophagy,
Год журнала:
2024,
Номер
20(6), С. 1213 - 1246
Опубликована: Март 6, 2024
Macroautophagy/autophagy
is
a
complex
degradation
process
with
dual
role
in
cell
death
that
influenced
by
the
types
are
involved
and
stressors
they
exposed
to.
Ferroptosis
an
iron-dependent
oxidative
form
of
characterized
unrestricted
lipid
peroxidation
context
heterogeneous
plastic
mechanisms.
Recent
studies
have
shed
light
on
involvement
specific
autophagy
(e.g.
ferritinophagy,
lipophagy,
clockophagy)
initiating
or
executing
ferroptotic
through
selective
anti-injury
proteins
organelles.
Conversely,
other
forms
reticulophagy
lysophagy)
enhance
cellular
defense
against
damage.
Dysregulated
autophagy-dependent
ferroptosis
has
implications
for
diverse
range
pathological
conditions.
This
review
aims
to
present
updated
definition
ferroptosis,
discuss
influential
substrates
receptors,
outline
experimental
methods,
propose
guidelines
interpreting
results.
Chemical Society Reviews,
Год журнала:
2024,
Номер
53(7), С. 3253 - 3272
Опубликована: Янв. 1, 2024
This
review
delineates
emerging
technologies
for
targeted
protein
degradation
that
directly
involve
lysosomes
or
proteasomes.
It
explores
their
unique
features,
advantages,
and
limitations,
offering
perspectives
on
future
therapeutic
applications.
Soft Matter,
Год журнала:
2025,
Номер
21(10), С. 1781 - 1812
Опубликована: Янв. 1, 2025
Peptide-mediated
liquid–liquid
phase
separation
(LLPS)
underpins
the
formation
of
dynamic
biomolecular
condensates,
regulated
by
diverse
molecular
interactions,
and
highlights
potential
applications
in
drug
delivery
synthetic
biology.
