Cytokine, Год журнала: 2024, Номер 185, С. 156820 - 156820
Опубликована: Ноя. 29, 2024
Язык: Английский
Preparative Biochemistry & Biotechnology, Год журнала: 2025, Номер unknown, С. 1 - 20
Опубликована: Март 14, 2025
Downstream processing continues to face significant bottlenecks due current purification technologies and improvements in upstream. Chromatography systems have been the primary method for their high yields purities. However, use of high-titer-producing strains has highlighted limitations chromatographic steps, including mass transfer limitations, low capacity, scalability issues. These challenges, combined with growing interest fully continuous manufacturing processes, led a widespread alternative affinity chromatography systems. Polyethylene glycol precipitation demonstrated be powerful, flexible, easily scalable, titer-independent methodology purifying therapeutic proteins such as monoclonal antibodies, achieving purities comparable Furthermore, it also holds great potential simplifying processes new modalities overcome downstream processing. Herein, we discuss latest advances polyethylene technology explore its future research directions applications.
Язык: Английский
Процитировано
0
International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142829 - 142829
Опубликована: Апрель 1, 2025
Exosomes are tiny extracellular membrane vesicles produced by various biological sources that have gained significant attention due to their theranostic potential. They carry macromolecules such as proteins, DNA, and RNA (including miRNAs) involved in intercellular communication within the central nervous system (CNS). This review summarizes role of exosomes miRNAs aspects depression, emphasizing therapeutic potential depression applications drug delivery systems. modulating critical pathways neurogenesis, synaptic plasticity, blood-brain barrier integrity, neuroinflammation, neural development. reduce neuroinflammation suppressing cytokine production immune activation. Furthermore, facilitate neuronal repair which is important for restoring circuitry, cognitive abilities, emotional mental stability affected depression. Moreover, exosomal emerging targets both diagnostic treatment perspectives treatment. The regulate gene expressions associated with functions health serve biomarkers novel approaches. In conclusion, may play a crucial will enhance probability successful
Язык: Английский
Процитировано
0
Osteoarthritis and Cartilage Open, Год журнала: 2025, Номер unknown, С. 100612 - 100612
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
Clínica e Investigación en Arteriosclerosis (English Edition), Год журнала: 2025, Номер 37(2), С. 100718 - 100718
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
International Journal of Molecular Medicine, Год журнала: 2024, Номер 54(4)
Опубликована: Авг. 7, 2024
Ovarian cancer (OC) is a common gynecological disease with high mortality rate worldwide due to its insidious nature and undetectability at an early stage. The standard treatment, combining platinum‑based chemotherapy cytoreductive surgery, has suboptimal results. Therefore, diagnosis of OC crucial. All cell types secrete extracellular vesicles, particularly exosomes. Exosomes, which contain lipids, proteins, DNA non‑coding RNAs (ncRNAs), are novel methods intercellular communication that participate in tumor development progression. ncRNAs categorized by size into long (lncRNAs) small (sncRNAs). sncRNAs further include transfer RNAs, nucleolar PIWI‑interacting microRNAs (miRNAs). miRNAs inhibit protein translation promote messenger RNA (mRNA) cleavage suppress gene expression. By sponging downstream miRNAs, lncRNAs circular can regulate target expression, thereby weakening the interactions between mRNAs. Exosomes exosomal ncRNAs, commonly present human biological fluids, promising biomarkers for OC. article aimed review potential role prognosis summarizing characteristics, processes, roles isolation exosomes ncRNAs.
Язык: Английский
Процитировано
3
Molecular and Cellular Biochemistry, Год журнала: 2024, Номер unknown
Опубликована: Дек. 19, 2024
Язык: Английский
Процитировано
3
Cancers, Год журнала: 2024, Номер 16(19), С. 3298 - 3298
Опубликована: Сен. 27, 2024
Salivary gland tumors (SGTs) are rare and diverse neoplasms, presenting significant challenges in diagnosis management due to their rarity complexity. Exosomes, lipid bilayer vesicles secreted by almost all cell types present body fluids, have emerged as crucial intercellular communication agents. They play multifaceted roles tumor biology, including modulating the microenvironment, promoting metastasis, influencing immune responses.
Язык: Английский
Процитировано
2
Clínica e Investigación en Arteriosclerosis, Год журнала: 2024, Номер unknown
Опубликована: Май 1, 2024
Atherosclerosis is the main pathogenic substrate for cardiovascular diseases (CVDs). Initially categorized as a passive cholesterol storage disease, nowadays, it considered an active process, identifying inflammation among key players its initiation and progression. Despite these advances, patients with CVDs are still at high risk of thrombotic events death, urging to deepen into molecular mechanisms underlying atherogenesis, identify novel diagnosis prognosis biomarkers their stratification. In this context, extracellular vesicles (EVs) have been postulated alternative in search atherosclerotic diseases, well investigate crosstalk between cells participating processes leading arterial remodelling. EVs nanosized lipidic particles released by most cell types physiological pathological conditions, that enclose lipids, proteins, nucleic acids from parental reflecting activation status. First cellular waste disposal systems, present, recognized effectors myriad processes, potential also CVDs. This review summarizes role CVDs, involvement atherosclerosis. La aterosclerosis es el principal sustrato patogénico de las enfermedades cardiovasculares (ECV). Considerada inicialmente como un depósito pasivo colesterol, hoy se asume que proceso activo, donde la inflamación juega papel clave en su inicio y progresión. A pesar los avances realizados, pacientes con ECV presentan alto riesgo episodios trombóticos elevada mortalidad, por lo sigue siendo necesario profundizar mecanismos aterogénesis e identificar nuevos biomarcadores diagnósticos pronósticos para estratificar riesgo. En este sentido, ha postulado vesículas extracelulares (EV) podrían representar enfermedad aterosclerótica, interés investigar participación comunicación intercelular favorece remodelado arterial. Las EV son partículas lipídicas liberadas numerosas células condiciones fisiológicas patológicas, contienen lípidos, proteínas ácidos nucleicos procedentes parentales. Consideradas material desecho, sabe efectores activos numerosos procesos celulares, constituyendo potenciales significado diagnóstico pronóstico ECV. esta revisión sintetiza favorecen aterosclerosis.
Процитировано
0
International review of cell and molecular biology, Год журнала: 2024, Номер unknown, С. 1 - 41
Опубликована: Сен. 6, 2024
Язык: Английский
Процитировано
0
Biomicrofluidics, Год журнала: 2024, Номер 18(5)
Опубликована: Сен. 1, 2024
The vascular network plays an essential role in the maintenance of all organs body via regulated delivery oxygen and nutrients, as well tissue communication transfer various biological signaling molecules. It also serves a route for drug administration affects pharmacokinetics. Due to this importance, engineers have sought create physiologically relevant reproducible systems tissue, considering cell-cell extracellular matrix interaction with structural physical conditions microenvironment. Extracellular vesicles (EVs) recently emerged important carriers transferring proteins genetic material between cells organs, delivery. Vascularized platforms can be ideal system studying interactions blood vessels EVs, which are crucial understanding EV-mediated substance situations. This review summarizes recent advances vascularized platforms, standard microfluidic-based techniques EV isolation characterization, studies EVs platforms. provides insights into EV-related (patho)physiological regulations facilitates development EV-based therapeutics.
Язык: Английский
Процитировано
0