Journal of Invertebrate Pathology, Год журнала: 2025, Номер unknown, С. 108328 - 108328
Опубликована: Апрель 1, 2025
Язык: Английский
Frontiers in Cellular and Infection Microbiology, Год журнала: 2021, Номер 11
Опубликована: Дек. 22, 2021
The increasing interest for Galleria mellonella larvae as an infection model is evidenced by the number of papers reporting its use, which increases exponentially since early 2010s. This popularity was initially linked to limitation conventional animal models due financial, technical and ethical aspects. In comparison, alternative (e.g. using Caenorhabditis elegans, Drosophila melanogaster or G. mellonella) were cheap, simple use not limited regulation. Since then, similar results have been established with comparatively vertebrates, it more often used a robust per se, only murine model. review attempts summarize current knowledge supporting development this model, both on immunological microbiological For that, we focus investigation virulence new therapies most important pathogenic bacteria. We also discuss points out directions standardization, well recent advances perspectives monitoring host-pathogen interactions.
Язык: Английский
Процитировано
131
Antibiotics, Год журнала: 2023, Номер 12(3), С. 505 - 505
Опубликована: Март 3, 2023
The first research on the insect Galleria mellonella was published 85 years ago, and larva is now widely used as a model to study infections caused by bacterial fungal pathogens, for screening new antimicrobials, adjacent immune response in co-infections or host-pathogen interaction, well toxicity model. system of G. shows remarkable similarities with mammals. Furthermore, results from correlate positively mammalian models other invertebrate models. Unlike models, can withstand temperatures 37 °C, its handling experimental procedures are simpler. Despite having some disadvantages, virtuous vivo be preclinical studies, an intermediate between vitro great example how apply bioethics principle 3Rs (Replacement, Reduction, Refinement) animal experimentation. This review aims discuss progress model, highlighting key aspects use, including design considerations necessity standardize them. A different score “cocoon” category included Health Index Scoring System also proposed.
Язык: Английский
Процитировано
45
Insects, Год журнала: 2023, Номер 14(3), С. 240 - 240
Опубликована: Фев. 27, 2023
Insects are, by far, the most common animals on our planet. The ubiquity and plethora of ecological niches occupied insects, along with strict sometimes forced coexistence between insects humans, make a target public health interest. This article reports negative aspects historically linked to as pests vectors diseases, describes their potential bioindicators environmental pollution, use food feed. Both positive impacts human animal need be addressed professionals who should aim strike balance within wide range conflicting goals in insect management, such regulating production, exploiting potential, protecting limiting impact humans. requires increased knowledge strategies preserve welfare. this paper is provide an overview traditional emerging topics bridging highlight for professionals, address these during work. present future role activities authorities regarding are analyzed.
Язык: Английский
Процитировано
40
Frontiers in Immunology, Год журнала: 2023, Номер 14
Опубликована: Июнь 16, 2023
Pattern recognition receptors (PRRs), as the “sensors” in immune response, play a prominent role recognizing pathogen-associated molecular patterns (PAMPs) and initiating an effective defense response to pathogens Lepidoptera. It is becoming increasingly clear that damage-associated (DAMPs) normally physiological within cells; however, when exposed extracellular, they may become “part-time” critical signals of response. Based on research recent years, we review herein typical PRRs Lepidoptera, including peptidoglycan protein (PGRP), gram-negative binding (GNBP), β-1,3-glucan (βGRP), C-type lectin (CTL), scavenger receptor (SR). We also outline ways which DAMPs participate correlation between escape. Taken together, these findings suggest insect innate immunity be much greater than expected it possible recognize broader range signaling molecules.
Язык: Английский
Процитировано
24
Pathogens, Год журнала: 2024, Номер 13(3), С. 233 - 233
Опубликована: Март 7, 2024
The study of pathogenicity and virulence fungal strains, in vivo the preclinical phase, is carried out through use animal models belonging to various classes mammals (rodents, leproids, etc.). Although animals are functionally more similar humans, these studies have some limitations terms ethics (animal suffering), user-friendliness, cost-effectiveness, timing (physiological response time) logistics (need for adequately equipped laboratories). A good model must possess optimal characteristics be used, such as rapid growth, small size short life cycle. For this reason, insects, Galleria mellonella (Lepidoptera), Drosophila melanogaster (Diptera) Bombyx mori been widely used alternative non-mammalian models. Due their simplicity low cost, larvae G. represent an above all evaluate pathogens antifungal treatments (either single or combination with biologically active compounds). further advantage also represented by simple neuronal system limiting suffering itself, ability survive at near-body ambient temperatures well expression proteins able recognise combined following three R principles (replacement, refinement reduction). This review aims assess validity advantages disadvantages replacing mammalian experimentation.
Язык: Английский
Процитировано
15
Cell, Год журнала: 2024, Номер unknown
Опубликована: Сен. 1, 2024
Язык: Английский
Процитировано
11
Pathogens and Disease, Год журнала: 2021, Номер 79(3)
Опубликована: Янв. 27, 2021
Infections caused by Klebsiella pneumoniae are a major public health threat. Extensively drug-resistant and even pan-resistant strains have been reported. Understanding K. pathogenesis is hampered the fact that murine models of infection offer limited resolution for non-hypervirulent which cause majority infections. The insect Galleria mellonella larva widely used alternative model organism bacterial pathogens. We performed genome-scale fitness profiling multidrug-resistant ST258 strain during G. mellonella, to determine if this suitable large-scale virulence factor discovery in pathogen. Our results demonstrated dominant role surface polysaccharides infection, with contributions from siderophores, cell envelope proteins, purine biosynthesis genes additional unknown function. Comparison hypervirulent strain, ATCC 43816, revealed substantial overlap important infection-related genes, as well putative factors specific ST258, reflecting strain-dependent effects. analysis also identified metalloregulatory protein NfeR (YqjI) virulence. Overall, study offers new insight into landscape pneumoniae, provides framework using highly flexible easily scalable dissect molecular mechanisms
Язык: Английский
Процитировано
44
Fermentation, Год журнала: 2022, Номер 8(9), С. 460 - 460
Опубликована: Сен. 14, 2022
Pigments from bacteria, fungi, yeast, cyanobacteria, and microalgae have been gaining more demand in the food, leather, textile industries due to their natural origin effective bioactive functions. Mass production of microbial pigments using inexpensive ecofriendly agro-industrial residues is current research low cost, origin, waste utilization, high pigment stimulating characteristics. A wide range substrates has employed submerged fermentation as carbon nitrogen sources enhance these microorganisms obtain required quantity pigments. Submerged proven yield when added with agro-waste residues. Hence, this review, aspects potential pigmented microbes such diversity, that stimulate a few under culture conditions, identification, ecological functions are detailed for benefit industrial personnel, researchers, other entrepreneurs explore multifaceted applications. In addition, some important covered herein disseminate knowledge.
Язык: Английский
Процитировано
32
Nanomaterials, Год журнала: 2025, Номер 15(1), С. 67 - 67
Опубликована: Янв. 3, 2025
The spread of multidrug-resistant microbes has made it necessary and urgent to develop new strategies deal with the infections they cause. Some these are based on nanotechnology, which revolutionized many fields in medicine. Evaluating safety efficacy antimicrobial requires testing animal models before being tested clinical trials. In this context, Galleria mellonella could represent a valid alternative traditional mammalian non-mammalian models, due its low cost, ease handling, valuable biological properties investigate host–pathogen interactions. purpose review is provide an updated overview literature concerning use G. larvae as model evaluate nanoparticles nanomaterials, particularly, those that used or under investigation combat microbial pathogens.
Язык: Английский
Процитировано
1
Food Hydrocolloids, Год журнала: 2025, Номер unknown, С. 111454 - 111454
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
1