Direct male development in chromosomally ZZ zebrafish
Frontiers in Cell and Developmental Biology,
Год журнала:
2024,
Номер
12
Опубликована: Март 11, 2024
The
genetics
of
sex
determination
varies
across
taxa,
sometimes
even
within
a
species.
Major
domesticated
strains
zebrafish
(
Danio
rerio
),
including
AB
and
TU,
lack
strong
genetic
determining
locus,
but
more
recently
derived
from
nature,
like
Nadia
(NA),
possess
ZZ
male/ZW
female
chromosomal
sex-determination
system.
fish
pass
through
juvenile
ovary
stage,
forming
oocytes
that
survive
in
become
females
die
males.
To
understand
mechanisms
gonad
development
NA
zebrafish,
we
studied
histology
single
cell
transcriptomics
developing
ZW
fish.
developed
by
22
days
post-fertilization
(dpf)
directly
formed
testes,
avoiding
phase.
Gonads
some
WW
fish,
however,
died
as
the
became
testis,
mimicking
suggesting
gynogenetically
strain
is
chromosomally
WW.
Single-cell
RNA-seq
19dpf
gonads
showed
similar
types
germ
cells,
precursors
gonadal
support
steroidogenic
interstitial/stromal
immune
consistent
with
bipotential
gonad.
In
contrast,
scRNA-seq
30dpf
revealed
cells
had
transcriptomes
characteristic
testicular
Sertoli,
Leydig,
while
granulosa
theca
oocytes.
Hematopoietic
vascular
were
both
genotypes.
These
results
show
initially
develop
gonad;
factor
on
W
chromosome,
or
fewer
than
two
Z
chromosomes,
essential
to
initiate
oocyte
development;
without
factor,
doses,
into
testes
passing
stage.
Sex
TU
mimics
loss
chromosome
during
domestication.
Genetic
analysis
will
facilitate
our
understanding
evolution
mechanisms.
Язык: Английский
tRNA expression and modification landscapes, and their dynamics during zebrafish embryo development
Nucleic Acids Research,
Год журнала:
2024,
Номер
52(17), С. 10575 - 10594
Опубликована: Июль 11, 2024
Abstract
tRNA
genes
exist
in
multiple
copies
the
genome
of
all
organisms
across
three
domains
life.
Besides
sequence
differences
copies,
extensive
post-transcriptional
modification
adds
a
further
layer
to
diversification.
Whilst
crucial
role
tRNAs
as
adapter
molecules
protein
translation
is
well
established,
whether
are
actually
expressed,
and
isodecoders
play
any
regulatory
only
recently
being
uncovered.
Here
we
built
upon
recent
developments
use
NGS-based
methods
for
RNA
detection
developed
tRAM-seq,
an
experimental
protocol
silico
analysis
pipeline
investigate
expression
modification.
Using
analysed
full
ensemble
nucleo-cytoplasmic
mitochondrial
during
embryonic
development
model
vertebrate
zebrafish.
We
show
that
repertoire
changes
development,
with
apparent
major
switch
isodecoder
profile
taking
place
around
start
gastrulation.
Taken
together,
our
findings
suggest
existence
general
reprogramming
expressed
pool,
possibly
gearing
translational
machinery
distinct
stages
delicate
process
embryo
development.
Язык: Английский
Transposon insertion causes ctnnb2 transcript instability that results in the maternal effect zebrafish ichabod (ich) mutation
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Март 4, 2025
Abstract
The
maternal-effect
mutation
ichabod
(
ich
)
results
in
ventralized
zebrafish
embryos
due
to
impaired
induction
of
the
dorsal
canonical
Wnt-signaling
pathway.
While
previous
studies
linked
phenotype
reduced
ctnnb2
transcript
levels,
causative
remained
unidentified.
Using
long-read
sequencing,
we
discovered
that
stems
from
insertion
a
non-autonomous
CMC-Enhancer/Suppressor-mutator
(CMC-EnSpm)
transposon
3’UTR
gene.
Through
reporter
assays,
demonstrate
while
wild
type
mRNAs
exhibit
remarkably
high
stability
throughout
early
stages
development,
dramatically
reduces
stability.
Genome-wide
mapping
CMC-EnSpm
transposons
across
multiple
strains
also
indicated
ongoing
transposition
activity
genome.
Our
findings
not
only
resolve
molecular
basis
but
highlight
continuing
mutagenic
potential
endogenous
and
reveal
unexpected
aspects
maternal
regulation
during
development.
Язык: Английский
The zebrafish (
PubMed,
Год журнала:
2025,
Номер
7(1), С. lqaf013 - lqaf013
Опубликована: Март 1, 2025
Small
nucleolar
RNAs
(snoRNAs)
are
one
of
the
most
abundant
and
evolutionary
ancient
group
functional
non-coding
RNAs.
They
were
originally
described
as
guides
post-transcriptional
rRNA
modifications,
but
emerging
evidence
suggests
that
snoRNAs
fulfil
an
impressive
variety
cellular
functions.
To
reveal
true
complexity
snoRNA-dependent
functions,
we
need
to
catalogue
first
complete
repertoire
in
a
given
context.
While
systematic
mapping
characterization
"snoRNAomes"
for
some
species
have
been
recently,
this
has
not
done
hitherto
zebrafish
(Danio
rerio).
Using
size-fractionated
RNA
sequencing
data
from
adult
tissues,
created
interactive
"snoRNAome"
database
species.
Our
custom-designed
analysis
pipeline
allowed
us
identify
with
high-confidence
67
previously
unannotated
genome,
resulting
set
date
Reanalyzing
multiple
published
datasets,
also
provide
dynamic
expression
during
early
stages
development
tissue-specific
patterns
others
adults.
facilitate
further
investigations
into
functions
zebrafish,
novel
database,
snoDanio,
which
can
be
used
explore
small
transcriptomic
data.
Язык: Английский
THE HYBRID HISTORY OF ZEBRAFISH
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 12, 2024
Abstract
Since
the
description
of
zebrafish
Danio
rerio
in
1822,
identity
its
closest
living
relative
has
been
unclear.
To
address
this
problem,
we
sequenced
exomes
ten
species
genus
and
used
them
to
infer
relationships
across
25
chromosomes
genome.
The
majority
within
were
remarkably
consistent
all
chromosomes.
Relationships
chromosome
segments,
however,
depended
systematically
upon
genomic
location
Regions
near
centers
identified
D.
kyathit
and/or
aesculapii
as
zebrafish,
while
segments
ends
supported
only
sister
species.
Genome-wide
comparisons
derived
character
states
revealed
that
danio
are
inconsistent
with
a
simple
bifurcating
history
support
an
ancient
hybrid
origin
lineage.
We
also
found
evidence
more
recent
gene
flow
limited
high
recombination
several
megabases
20
distinct
from
rest
additional
insight
gained
incorporating
genome
structure
into
phylogenomic
study
demonstrates
utility
such
approach
for
future
studies
other
taxa.
multiple
histories
have
important
implications
comparative
these
our
understanding
evolutionary
zebrafish.
Язык: Английский
miR214 regulates sex determination through gsdf in zebrafish
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Май 3, 2024
Abstract
Sex
determination
is
a
variable
and
complex
mechanism,
yet
it
can
be
found
all
over
the
plant
animal
kingdoms.
It
creates
two
morphological
different
outcomes
from
one
same
species.
Our
work
demonstrates
involvement
of
non-coding
RNA
dnm3os
,
its
embedded
microRNA
miR214
in
this
process
for
teleost
Danio
rerio
.
First,
we
find
that
acts
through
gsdf
to
drive
female
development.
Second,
additional
alleles
revealed
lncRNA
also
promote
male
development
unknown
mechanisms.
Finally,
describe
sex-determining
activities
display
maternal
effect,
suggesting
imbalances
gene-regulatory
system
compensated
stable
manner.
Although
cannot
fully
explain
complexity
mechanisms
have
started
reveal,
our
once
again
highlights
flexibility
sex
identifies
miRNA
other
mediated
crucial
process.
Язык: Английский