Variant ribosomal DNA is essential for female differentiation in zebrafish
Philosophical Transactions of the Royal Society B Biological Sciences,
Год журнала:
2025,
Номер
380(1921)
Опубликована: Март 6, 2025
The
ribosome
consists
of
protein
and
RNA
components.
Deletion
genes
encoding
specific
ribosomal
proteins
has
revealed
that
heterogeneity
in
the
must
exist
vertebrates;
however,
this
not
been
tested
for
(rRNA).
In
zebrafish
(Danio
rerio),
'45S-M'
RNA-encoding
locus
undergoes
massive
extrachromosomal
amplification
during
oocyte
growth
ovary
differentiation
is
distinct
from
regular
DNA
(rDNA)
somatic
rRNA
(45S-S).
Although
45S-M
rDNA
falls
within
only
described
sex-linked
region
multiple
wild
strains,
its
role
sexual
unclear.
We
used
CRISPR-Cas9
gene
editing
to
alter
sequences
zygotes
found
although
there
was
no
effect
on
or
male
development,
dramatic
suppression
female
differentiation.
Males
with
edited
produced
phenotypically
normal
sperm
were
able
fertilize
eggs
wild-type
females,
resulting
embryos
once
more
displaying
development.
Our
work
supports
hypothesis
specialized
elusive
apical
sex-determining
represents
most
tractable
genetic
system
date
studying
function
a
vertebrate.This
article
part
discussion
meeting
issue
'Ribosome
diversity
impact
synthesis,
development
disease'.
Язык: Английский
A maternal-to-zygotic-transition gene block on the zebrafish sex chromosome
G3 Genes Genomes Genetics,
Год журнала:
2024,
Номер
14(5)
Опубликована: Март 11, 2024
Wild
zebrafish
(Danio
rerio)
have
a
ZZ/ZW
chromosomal
sex-determination
system
with
the
major
sex
locus
on
right
arm
of
chromosome-4
(Chr4R)
near
largest
heterochromatic
block
in
genome,
suggesting
that
Chr4R
transcriptomics
might
differ
from
rest
genome.
To
test
this
hypothesis,
we
conducted
an
RNA-seq
analysis
adult
ZW
ovaries
and
ZZ
testes
Nadia
strain
identified
4
regions
Chr4
different
gene
expression
profiles.
Unique
protein-coding
genes
41.7
Mb
section
(Region-2)
were
expressed
testis
but
silent
ovary.
The
AB
lab
strain,
which
lacks
chromosomes,
verified
result,
showing
testis-biased
Region-2
depends
gonad
biology,
not
sex-determining
mechanism.
analyses
female
male
brains
livers
validated
reduced
transcripts
somatic
cells,
without
specificity.
corresponds
to
portion
its
content
repetitive
elements
distinguishes
it
human
orthologs;
has
zinc
finger
early
zygotic
genome
activation;
maternal
5S
rRNA
genes,
spliceosome
concentration
tRNA
distinct
set
elements.
colocalization
(1)
silenced
are
(2)
embryos
briefly
at
onset
(3)
maternal-specific
for
translation
machinery;
(4)
components;
(5)
adjacent
encoding
miR-430,
mediates
transcript
degradation,
suggest
is
maternal-to-zygotic-transition
regulatory
block.
Язык: Английский
Rbpms2 promotes female fate upstream of the nutrient sensing Gator2 complex component Mios
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Июнь 19, 2024
Abstract
Reproductive
success
relies
on
proper
establishment
and
maintenance
of
biological
sex.
In
many
animals,
including
mammals,
the
primary
gonad
is
initially
ovary
biased.
We
previously
showed
RNA
binding
protein
(RNAbp),
Rbpms2,
required
for
fate
in
zebrafish.
Here,
we
identified
Rbpms2
targets
oocytes
(Rbpms2-bound
oocyte
RNAs;
rboRNAs
).
identify
as
a
translational
regulator
,
which
include
testis
factors
ribosome
biogenesis
factors.
Further,
genetic
analyses
indicate
that
promotes
nucleolar
amplification
via
mTorc1
signaling
pathway,
specifically
through
mTorc1-activating
Gap
activity
towards
Rags
2
(Gator2)
component,
Missing
(Mios).
Cumulatively,
our
findings
early
gonocytes
are
dual
poised,
bipotential
state
acts
binary
fate-switch.
Specifically,
represses
to
promote
progression
an
essential
Gator2-mediated
checkpoint,
thereby
integrating
regulation
sexual
differentiation
nutritional
availability
pathways
zebrafish
oogenesis.
Язык: Английский
<i>foxl2l</i> is a germ cell-intrinsic gatekeeper of oogenesis in zebrafish
动物学研究,
Год журнала:
2024,
Номер
45(5), С. 1116 - 1130
Опубликована: Янв. 1, 2024
Zebrafish
serve
as
a
valuable
model
organism
for
studying
germ
cell
biology
and
reproductive
processes.
The
AB
strain
of
zebrafish
is
proposed
to
exhibit
polygenic
sex
determination
system,
where
most
males
initially
develop
juvenile
ovaries
before
committing
male
fate.
In
species
with
chromosomal
determination,
gonadal
somatic
cells
are
recognized
key
determinants
Notably,
the
loss
in
leads
masculinization,
implying
that
harbor
an
intrinsic
feminization
signal.
However,
specific
signal
triggering
oogenesis
remains
unclear.
present
study,
we
identified
Язык: Английский
THE HYBRID HISTORY OF ZEBRAFISH
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 12, 2024
Abstract
Since
the
description
of
zebrafish
Danio
rerio
in
1822,
identity
its
closest
living
relative
has
been
unclear.
To
address
this
problem,
we
sequenced
exomes
ten
species
genus
and
used
them
to
infer
relationships
across
25
chromosomes
genome.
The
majority
within
were
remarkably
consistent
all
chromosomes.
Relationships
chromosome
segments,
however,
depended
systematically
upon
genomic
location
Regions
near
centers
identified
D.
kyathit
and/or
aesculapii
as
zebrafish,
while
segments
ends
supported
only
sister
species.
Genome-wide
comparisons
derived
character
states
revealed
that
danio
are
inconsistent
with
a
simple
bifurcating
history
support
an
ancient
hybrid
origin
lineage.
We
also
found
evidence
more
recent
gene
flow
limited
high
recombination
several
megabases
20
distinct
from
rest
additional
insight
gained
incorporating
genome
structure
into
phylogenomic
study
demonstrates
utility
such
approach
for
future
studies
other
taxa.
multiple
histories
have
important
implications
comparative
these
our
understanding
evolutionary
zebrafish.
Язык: Английский
miR214 regulates sex determination through gsdf in zebrafish
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Май 3, 2024
Abstract
Sex
determination
is
a
variable
and
complex
mechanism,
yet
it
can
be
found
all
over
the
plant
animal
kingdoms.
It
creates
two
morphological
different
outcomes
from
one
same
species.
Our
work
demonstrates
involvement
of
non-coding
RNA
dnm3os
,
its
embedded
microRNA
miR214
in
this
process
for
teleost
Danio
rerio
.
First,
we
find
that
acts
through
gsdf
to
drive
female
development.
Second,
additional
alleles
revealed
lncRNA
also
promote
male
development
unknown
mechanisms.
Finally,
describe
sex-determining
activities
display
maternal
effect,
suggesting
imbalances
gene-regulatory
system
compensated
stable
manner.
Although
cannot
fully
explain
complexity
mechanisms
have
started
reveal,
our
once
again
highlights
flexibility
sex
identifies
miRNA
other
mediated
crucial
process.
Язык: Английский
Adamts9 is required for the development of primary ovarian follicles and maintenance of female sex in zebrafish
Biology of Reproduction,
Год журнала:
2024,
Номер
111(5), С. 1107 - 1128
Опубликована: Авг. 24, 2024
Abstract
Previous
studies
have
suggested
that
adamts9
(a
disintegrin
and
metalloprotease
with
thrombospondin
type-1
motifs,
member
9),
an
extracellular
matrix
(ECM)
metalloprotease,
participates
in
primordial
germ
cell
(PGC)
migration
is
necessary
for
female
fertility.
In
this
study,
we
found
knockout
(KO)
led
to
reduced
body
size,
female-to-male
sex
conversion
late
juvenile
or
adult
zebrafish;
however,
primary
determination
was
not
affected
early
juveniles
of
KO.
Overfeeding
lowering
the
rearing
density
rescued
growth
defects
KO
fish
but
did
rescue
ovarian
development
Delayed
PGC
proliferation,
significantly
number
size
Stage
IB
follicles
(equivalent
follicles)
KO,
arrested
at
mid-
late-juveniles
are
likely
causes
infertility
conversion.
Via
RNAseq,
significant
enrichment
differentially
expressed
genes
involved
ECM
organization
during
sexual
maturation
ovaries
wildtype
fish;
dysregulation
these
ovaries.
RNAseq
analysis
also
showed
inflammatory
transcriptomic
signatures
Taken
together,
our
results
indicate
critical
maintenance
sex,
loss
leads
follicle
development,
infertility,
mature
adults.
These
show
metalloproteases
play
major
roles
maintaining
zebrafish.
Язык: Английский
Two phases of gonadal sex differentiation in zebrafish with ZZ/ZW sex determination system
General and Comparative Endocrinology,
Год журнала:
2024,
Номер
unknown, С. 114613 - 114613
Опубликована: Сен. 1, 2024
Язык: Английский
Zebrafish as a model system for studying reproductive diseases
Wenwen Zha,
Weitao Hu,
Chenkai Ge
и другие.
Frontiers in Cell and Developmental Biology,
Год журнала:
2024,
Номер
12
Опубликована: Дек. 23, 2024
Reproductive
system
diseases
have
become
a
major
health
challenge
facing
humans,
so
extensive
investigations
are
needed
to
understand
their
complex
pathogenesis
and
summarize
effective
treatments.
In
the
study
of
reproductive
diseases,
mice
most
commonly
used
animal
model.
However,
cost
time
required
establish
mouse
models
high.
The
existing
zebrafish
model
can
solve
this
problem
well.
Zebrafish
is
an
with
great
application
prospects
has
lots
advantages,
including
high
degree
genetic
conservation
short
cycle,
transparent
embryos,
rapid
growth,
providing
unique
opportunities
for
high-throughput
drug
screening
identification
potential
Researchers
successfully
chemical
induction,
physical
damage,
gene
editing
technology,
etc.,
induce
damage
in
biological
processes
related
its
diseases.
Therefore,
review,
main
advantages
summarized,
pathological
mechanisms
as
disease
clarified,
new
perspectives
valuable
insights
provided
treatment
human
literature
data
cited
review
all
from
PubMed,
covering
important
research
results
on
past
10
years.
Язык: Английский