Direct male development in chromosomally ZZ zebrafish

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: March 11, 2024

The genetics of sex determination varies across taxa, sometimes even within a species. Major domesticated strains zebrafish ( Danio rerio ), including AB and TU, lack strong genetic determining locus, but more recently derived from nature, like Nadia (NA), possess ZZ male/ZW female chromosomal sex-determination system. fish pass through juvenile ovary stage, forming oocytes that survive in become females die males. To understand mechanisms gonad development NA zebrafish, we studied histology single cell transcriptomics developing ZW fish. developed by 22 days post-fertilization (dpf) directly formed testes, avoiding phase. Gonads some WW fish, however, died as the became testis, mimicking suggesting gynogenetically strain is chromosomally WW. Single-cell RNA-seq 19dpf gonads showed similar types germ cells, precursors gonadal support steroidogenic interstitial/stromal immune consistent with bipotential gonad. In contrast, scRNA-seq 30dpf revealed cells had transcriptomes characteristic testicular Sertoli, Leydig, while granulosa theca oocytes. Hematopoietic vascular were both genotypes. These results show initially develop gonad; factor on W chromosome, or fewer than two Z chromosomes, essential to initiate oocyte development; without factor, doses, into testes passing stage. Sex TU mimics loss chromosome during domestication. Genetic analysis will facilitate our understanding evolution mechanisms.

Language: Английский

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tRNA expression and modification landscapes, and their dynamics during zebrafish embryo development

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: 52(17), P. 10575 - 10594

Published: July 11, 2024

Abstract tRNA genes exist in multiple copies the genome of all organisms across three domains life. Besides sequence differences copies, extensive post-transcriptional modification adds a further layer to diversification. Whilst crucial role tRNAs as adapter molecules protein translation is well established, whether are actually expressed, and isodecoders play any regulatory only recently being uncovered. Here we built upon recent developments use NGS-based methods for RNA detection developed tRAM-seq, an experimental protocol silico analysis pipeline investigate expression modification. Using analysed full ensemble nucleo-cytoplasmic mitochondrial during embryonic development model vertebrate zebrafish. We show that repertoire changes development, with apparent major switch isodecoder profile taking place around start gastrulation. Taken together, our findings suggest existence general reprogramming expressed pool, possibly gearing translational machinery distinct stages delicate process embryo development.

Language: Английский

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Transposon insertion causes ctnnb2 transcript instability that results in the maternal effect zebrafish ichabod (ich) mutation

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Abstract The maternal-effect mutation ichabod ( ich ) results in ventralized zebrafish embryos due to impaired induction of the dorsal canonical Wnt-signaling pathway. While previous studies linked phenotype reduced ctnnb2 transcript levels, causative remained unidentified. Using long-read sequencing, we discovered that stems from insertion a non-autonomous CMC-Enhancer/Suppressor-mutator (CMC-EnSpm) transposon 3’UTR gene. Through reporter assays, demonstrate while wild type mRNAs exhibit remarkably high stability throughout early stages development, dramatically reduces stability. Genome-wide mapping CMC-EnSpm transposons across multiple strains also indicated ongoing transposition activity genome. Our findings not only resolve molecular basis but highlight continuing mutagenic potential endogenous and reveal unexpected aspects maternal regulation during development.

Language: Английский

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The zebrafish (

PubMed, Journal Year: 2025, Volume and Issue: 7(1), P. lqaf013 - lqaf013

Published: March 1, 2025

Small nucleolar RNAs (snoRNAs) are one of the most abundant and evolutionary ancient group functional non-coding RNAs. They were originally described as guides post-transcriptional rRNA modifications, but emerging evidence suggests that snoRNAs fulfil an impressive variety cellular functions. To reveal true complexity snoRNA-dependent functions, we need to catalogue first complete repertoire in a given context. While systematic mapping characterization "snoRNAomes" for some species have been recently, this has not done hitherto zebrafish (Danio rerio). Using size-fractionated RNA sequencing data from adult tissues, created interactive "snoRNAome" database species. Our custom-designed analysis pipeline allowed us identify with high-confidence 67 previously unannotated genome, resulting set date Reanalyzing multiple published datasets, also provide dynamic expression during early stages development tissue-specific patterns others adults. facilitate further investigations into functions zebrafish, novel database, snoDanio, which can be used explore small transcriptomic data.

Language: Английский

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THE HYBRID HISTORY OF ZEBRAFISH

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 12, 2024

Abstract Since the description of zebrafish Danio rerio in 1822, identity its closest living relative has been unclear. To address this problem, we sequenced exomes ten species genus and used them to infer relationships across 25 chromosomes genome. The majority within were remarkably consistent all chromosomes. Relationships chromosome segments, however, depended systematically upon genomic location Regions near centers identified D. kyathit and/or aesculapii as zebrafish, while segments ends supported only sister species. Genome-wide comparisons derived character states revealed that danio are inconsistent with a simple bifurcating history support an ancient hybrid origin lineage. We also found evidence more recent gene flow limited high recombination several megabases 20 distinct from rest additional insight gained incorporating genome structure into phylogenomic study demonstrates utility such approach for future studies other taxa. multiple histories have important implications comparative these our understanding evolutionary zebrafish.

Language: Английский

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miR214 regulates sex determination through gsdf in zebrafish

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 3, 2024

Abstract Sex determination is a variable and complex mechanism, yet it can be found all over the plant animal kingdoms. It creates two morphological different outcomes from one same species. Our work demonstrates involvement of non-coding RNA dnm3os , its embedded microRNA miR214 in this process for teleost Danio rerio . First, we find that acts through gsdf to drive female development. Second, additional alleles revealed lncRNA also promote male development unknown mechanisms. Finally, describe sex-determining activities display maternal effect, suggesting imbalances gene-regulatory system compensated stable manner. Although cannot fully explain complexity mechanisms have started reveal, our once again highlights flexibility sex identifies miRNA other mediated crucial process.

Language: Английский

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