Horticulturae,
Год журнала:
2024,
Номер
10(12), С. 1305 - 1305
Опубликована: Дек. 7, 2024
ALMT
(aluminum-activated
malate
transporter)
proteins
play
crucial
roles
in
the
transport
of
organic
acids
and
have
significant
implications
for
plant
stress
responses
development.
While
extensively
studied
some
plants,
characteristics
functional
divergence
gene
family
not
yet
been
thoroughly
explored
Prunus
mume
other
Rosaceae
species.
In
this
study,
we
systematically
analyzed
across
nine
species
to
explore
their
evolutionary
relationships,
structural
characteristics,
roles.
A
total
138
genes
were
identified
categorized
into
four
groups
based
on
a
phylogenetic
analysis.
The
motif
analysis
confirmed
accuracy
grouping.
collinearity
indicated
that
whole-genome
duplication
events
primary
drivers
expansion
these
Furthermore,
cis-acting
element
revealed
diverse
regulatory
elements
associated
with
environmental
responses,
including
abscisic
acid,
light,
jasmonic
acid.
expression
correlation
showed
PmALMT1
is
primarily
malic
acid
accumulation,
whereas
PmALMT8
related
citric
accumulation.
Further
transient
Nicotiana
benthamiana
validated
above
conclusion.
This
comprehensive
provides
valuable
insights
evolution,
function,
regulation
PLANT PHYSIOLOGY,
Год журнала:
2024,
Номер
195(4), С. 2799 - 2814
Опубликована: Май 14, 2024
Abstract
The
cultivated
apple
(Malus
domestica
Borkh.)
is
a
cross-pollinated
perennial
fruit
tree
of
great
economic
importance.
Earlier
versions
reference
genomes
were
unphased,
fragmented,
and
lacked
comprehensive
insights
into
the
apple's
highly
heterozygous
genome,
which
impeded
advances
in
genetic
studies
breeding
programs.
In
this
study,
we
assembled
haplotype-resolved
telomere-to-telomere
(T2T)
genome
for
diploid
cultivar
Golden
Delicious.
Subsequently,
constructed
pangenome
based
on
12
assemblies
from
wild
species
to
investigate
dynamic
changes
functional
genes.
Our
results
revealed
gene
gain
loss
events
during
domestication.
Compared
with
species,
more
families
significantly
enriched
oxidative
phosphorylation,
pentose
metabolic
process,
responses
salt,
abscisic
acid
biosynthesis
process.
analyses
also
demonstrated
higher
prevalence
different
types
resistance
analogs
(RGAs)
cultivars
than
their
relatives,
partially
attributed
segmental
tandem
duplication
certain
RGAs
classes.
Structural
variations,
mainly
deletions
insertions,
have
affected
presence
absence
TIR-NB-ARC-LRR,
NB-ARC-LRR,
CC-NB-ARC-LRR
Additionally,
hybridization/introgression
has
contributed
expansion
genes
domesticated
apples.
T2T
provide
important
resources
apples,
emphasizing
need
study
evolutionary
mechanisms
breeding.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Март 27, 2024
Abstract
Telomeres
play
an
important
role
in
chromosome
stability
and
their
length
is
thought
to
be
related
organism’s
lifestyle
lifespan.
Telomere
variable
across
plant
species
between
cultivars
of
the
same
species,
possibly
conferring
adaptive
advantage.
However,
it
not
known
whether
telomere
or
life
span
a
diverse
array
due
lack
information
on
plants.
Here
we
leverage
genomes
assembled
with
long
read
sequencing
data
estimate
by
chromosome.
We
find
that
assemblies
based
Oxford
Nanopore
Technologies
(ONT)
accurately
predict
two
model
Arabidopsis
thaliana
Oryza
sativa
matching
lab-based
estimates.
then
different
lifestyles
lifespans
general
gymnosperms
have
shorter
telomeres
compared
eudicots
monocots.
Crop
frequently
longer
than
wild
relatives,
been
maintained
clonally
such
as
hemp
reflecting
this
requires
term
chromosomal
stability.
Horticulture Research,
Год журнала:
2024,
Номер
11(5)
Опубликована: Март 14, 2024
Abstract
Musa
ornata
and
velutina
are
members
of
the
Musaceae
family
indigenous
to
South
Southeast
Asia.
They
very
popular
in
horticultural
market,
but
lack
genomic
sequencing
data
genetic
studies
has
hampered
efforts
improve
their
ornamental
value.
In
this
study,
we
generated
first
chromosome-level
genome
assemblies
for
both
species
by
utilizing
Oxford
Nanopore
long
reads
Hi-C
reads.
The
genomes
M.
were
assembled
into
11
pseudochromosomes
with
sizes
427.85
Mb
478.10
Mb,
respectively.
Repetitive
sequences
comprised
46.70%
50.91%
total
velutina,
Differentially
expressed
gene
(DEG)
Gene
Ontology
(GO)
enrichment
analyses
indicated
that
upregulated
genes
mature
pericarps
mainly
associated
saccharide
metabolic
processes,
particularly
at
cell
wall
extracellular
region.
Furthermore,
identified
polygalacturonase
(PG)
exhibited
higher
expression
level
compared
other
tissues,
potentially
being
accountable
pericarp
dehiscence.
This
study
also
anthocyanin
biosynthesis
pathway.
Taken
together,
chromosomal-level
provide
valuable
insights
mechanism
dehiscence
banana,
which
will
significantly
contribute
future
molecular
breeding
efforts.
Ornamental Plant Research,
Год журнала:
2024,
Номер
4(1), С. 0 - 0
Опубликована: Янв. 1, 2024
Rosaceae
plants
are
widely
distributed
worldwide.
These
plants,
including
well-known
ornamental
and
those
producing
temperate
fruits,
have
significant
economic
value.
The
utilization
of
omics
technologies
has
significantly
advanced
the
research
progress
on
in
various
areas
germplasm
resources,
molecular
breeding,
evolutionary
domestication.
Here,
we
provide
an
overview
applications
Rosaceae.
It
includes
whole-genome
sequencing
assembly
as
well
genome-wide
association
studies,
transcriptome,
proteomics,
metabolomics,
comparative
genomics.
techniques
used
to
understand
genetic
regulatory
mechanisms
underlying
important
traits
such
flower
color,
fragrance,
stress
tolerance,
fruit
quality.
We
outline
prospects
genomics
aiming
establish
a
foundation
for
comprehending
breeding
traits.
This
could
potentially
theoretical
support
rapidly
cultivating
new
varieties.
The Plant Journal,
Год журнала:
2024,
Номер
119(4), С. 1937 - 1952
Опубликована: Июнь 25, 2024
SUMMARY
Flavonols
are
widely
synthesized
throughout
the
plant
kingdom,
playing
essential
roles
in
physiology
and
providing
unique
health
benefits
for
humans.
Their
glycosylation
plays
significant
role
improving
their
stability
solubility,
thus
accumulation
function.
However,
genes
encoding
enzymes
catalyze
this
remain
largely
unknown
apple.
This
study
utilized
a
combination
of
methods
to
identify
such
enzymes.
Initially,
candidate
were
selected
based
on
potential
encode
UDP‐dependent
glycosyltransferases
(UGTs)
expression
patterns
response
light
induction.
Subsequently,
through
testing
vitro
enzyme
activity
proteins
produced
Escherichia
coli
cells,
four
candidates
confirmed
flavonol
3‐
O
‐galactosyltransferase
(UGT78T6),
‐glucosyltransferase
(UGT78S1),
‐xylosyltransferase/arabinosyltransferase
(UGT78T5),
‐rhamnosyltransferase
(UGT76AE22),
respectively.
Further
validation
these
genes'
functions
was
conducted
by
modulating
levels
stably
transformed
apple
plants.
As
anticipated,
positive
correlation
observed
between
content
specific
glycosides
corresponding
each
gene.
Moreover,
overexpression
synthase
gene,
MdFLS
,
resulted
increased
glycoside
roots
leaves.
These
findings
provide
valuable
insights
breeding
programs
aimed
at
enriching
flesh
with
flavonols
identifying
‐glycosyltransferases
other
species.
Agronomy,
Год журнала:
2024,
Номер
15(1), С. 58 - 58
Опубликована: Дек. 28, 2024
Fruit
quality
is
one
of
the
most
important
economic
traits
fruit
crops,
directly
influencing
market
prices
and
orchard
revenues.
Enhancing
has
therefore
become
a
critical
objective
in
both
production
scientific
research.
External
fruits
typically
include
size,
coloration,
shape,
uniformity,
consistency.
Internal
refer
to
nutritional
value
fruits,
which
largely
determine
their
flavor
palatability.
Over
past
decade,
significant
progress
been
made
understanding
formation
regulatory
mechanisms
quality,
with
numerous
key
functional
genes
associated
being
identified.
While
substantial
advancements
have
achieved
studying
specific
aspects
there
remains
lack
comprehensive
systematic
reviews
addressing
overall
physiology
interplay
among
various
traits,
diversity
mechanisms.
Using
Chinese
pears
as
an
example,
this
review
summarizes
research
regulation
over
five
years.
Key
metabolic
such
sweetness,
color,
texture,
physiological
disorders;
factors
stone
cell
formation;
sugar
content
regulation;
roles
plant
hormones
including
ethylene,
gibberellins,
abscisic
acid;
translational
post-translational
modifications
ubiquitination,
methylation,
acetylation;
well
application
genomic
sequencing
technologies.
Furthermore,
offers
practical
suggestions
for
improving
pear
provides
insights
researchers
related
fields.
Finally,
future
trends
are
discussed,
offering
forward-looking
perspective
advancing
field.
Horticulturae,
Год журнала:
2025,
Номер
11(1), С. 106 - 106
Опубликована: Янв. 20, 2025
Located
in
the
southwestern
region
of
China,
Yunnan–Kweichow
Plateau,
is
closely
related
to
origins
Pyrus
L.
Despite
this
important
status,
there
has
been
relatively
little
population
genetics
research
focused
on
wild
species
area.
To
address
gap
knowledge,
present
study
was
conducted
investigating
distributions
Plateau
region.
These
analyses
entailed
collection
80
accessions,
whole-genome
resequencing,
and
detection
variants
including
SNPs,
InDels,
SVs,
CNVs.
Genetic
structure
revealed
clear
differences
between
P.
pashia
calleryana,
where
former
additionally
subdivided
into
five
groups.
The
genetic
these
accessions
aligned
with
their
geographic
distribution,
highlighting
fragmented
nature
populations
Plateau.
Analyses
diversity
suggested
that
central
easter
portions
Yunnan
Province
are
key
centers
whereas
highest
degree
differentiation
observed
for
southwest
northwest
regions.
Demographic
indicated
currently
a
state
contraction,
evidence
migration
events
populations.
