The winding road: Infectious disease considerations for CAR-T and other novel adoptive cellular therapies in the era of COVID-19
Seminars in Hematology,
Год журнала:
2024,
Номер
61(5), С. 321 - 332
Опубликована: Авг. 23, 2024
Язык: Английский
Altered spike IgG Fc N-linked glycans are associated with hyperinflammatory state in adult COVID and Multisystem Inflammatory Syndrome in Children
medRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 14, 2024
Abstract
Background
Severe
COVID
and
multisystem
inflammatory
syndrome
(MIS-C)
are
characterized
by
excessive
cytokines/chemokines.
In
adults,
disease
severity
is
associated
with
SARS-CoV-2-specific
IgG
Fc
afucosylation,
which
induces
pro-inflammatory
cytokine
secretion
from
innate
immune
cells.
This
study
aimed
to
define
spike
glycosylation
following
SARS-CoV-2
infection
in
adults
children
vaccination
the
relationships
between
glycan
modifications
cytokine/chemokine
levels.
Methods
We
analyzed
longitudinal
(n=146)
cross-sectional
(n=49)
serum/plasma
samples
adult
pediatric
patients,
MIS-C
vaccinees,
healthy
controls.
developed
methods
for
characterizing
bulk
capillary
electrophoresis
(CE)
measured
levels
of
ten
cytokines/chemokines
multiplexed
ELISA.
Results
Spike
were
more
afucosylated
than
during
acute
MIS-C.
observed
an
opposite
trend
vaccination,
but
it
was
not
significant.
galactosylated
sialylated
less
bisected
COVID,
similar
trends
COVID/MIS-C
vaccination.
changed
time
or
Afucosylated
exhibited
inverse
positive
correlations
markers
respectively;
inversely
correlated
cytokines
MIS-C;
positively
multiple
groups.
Conclusions
identified
previously
undescribed
that
expand
our
understanding
changes
may
impact
immunopathology.
Язык: Английский
Severe Acute Respiratory Syndrome Coronavirus 2 Plasma Antibody and Nucleocapsid Antigen Status Predict Outcomes in Outpatients With Coronavirus Disease 2019
Clinical Infectious Diseases,
Год журнала:
2024,
Номер
79(4), С. 920 - 927
Опубликована: Июль 17, 2024
Reliable
biomarkers
of
coronavirus
disease
2019
(COVID-19)
outcomes
are
critically
needed.
We
evaluated
associations
spike
antibody
(Ab)
and
plasma
nucleocapsid
antigen
(N
Ag)
with
clinical
in
nonhospitalized
persons
mild-to-moderate
COVID-19.
Язык: Английский
Altered spike IgG Fc N-linked glycans are associated with hyperinflammatory state in adult COVID-19 and Multisystem Inflammatory Syndrome in Children
Open Forum Infectious Diseases,
Год журнала:
2024,
Номер
11(11)
Опубликована: Окт. 16, 2024
Abstract
Background
Severe
coronavirus
disease
2019
(COVID-19)
and
multisystem
inflammatory
syndrome
(MIS-C)
are
characterized
by
excessive
cytokines/chemokines.
In
adults,
severity
is
associated
with
severe
acute
respiratory
2
(SARS-CoV-2)–specific
immunoglobulin
G
(IgG)
Fc
afucosylation,
which
induces
proinflammatory
cytokine
secretion
from
innate
immune
cells.
This
study
aimed
to
define
spike
IgG
glycosylation
following
SARS-CoV-2
infection
in
adults
children
vaccination
the
relationships
between
glycan
modifications
Methods
We
analyzed
longitudinal
(n
=
146)
cross-sectional
49)
serum/plasma
samples
adult
pediatric
COVID-19
patients,
MIS-C
vaccinees,
controls.
developed
methods
for
characterizing
bulk
capillary
electrophoresis
measured
levels
of
10
cytokines/chemokines
multiplexed
enzyme-linked
immunosorbent
assay.
Results
Spike
was
more
afucosylated
than
during
MIS-C.
observed
an
opposite
trend
vaccination,
but
it
not
significant.
galactosylated
sialylated
less
bisected
COVID-19,
similar
trends
COVID-19/MIS-C
vaccination.
changed
time
or
Afucosylated
exhibited
inverse
positive
correlations
markers
respectively;
inversely
correlated
cytokines
MIS-C;
positively
multiple
groups.
Conclusions
identified
previously
undescribed
that
expand
our
understanding
changes
may
impact
immunopathology.
Язык: Английский