Altered spike IgG Fc N-linked glycans are associated with hyperinflammatory state in adult COVID-19 and Multisystem Inflammatory Syndrome in Children DOI Creative Commons
Jacob D. Sherman,

Vinit Karmali,

Bhoj Kumar

et al.

Open Forum Infectious Diseases, Journal Year: 2024, Volume and Issue: 11(11)

Published: Oct. 16, 2024

Abstract Background Severe coronavirus disease 2019 (COVID-19) and multisystem inflammatory syndrome (MIS-C) are characterized by excessive cytokines/chemokines. In adults, severity is associated with severe acute respiratory 2 (SARS-CoV-2)–specific immunoglobulin G (IgG) Fc afucosylation, which induces proinflammatory cytokine secretion from innate immune cells. This study aimed to define spike IgG glycosylation following SARS-CoV-2 infection in adults children vaccination the relationships between glycan modifications Methods We analyzed longitudinal (n = 146) cross-sectional 49) serum/plasma samples adult pediatric COVID-19 patients, MIS-C vaccinees, controls. developed methods for characterizing bulk capillary electrophoresis measured levels of 10 cytokines/chemokines multiplexed enzyme-linked immunosorbent assay. Results Spike was more afucosylated than during MIS-C. observed an opposite trend vaccination, but it not significant. galactosylated sialylated less bisected COVID-19, similar trends COVID-19/MIS-C vaccination. changed time or Afucosylated exhibited inverse positive correlations markers respectively; inversely correlated cytokines MIS-C; positively multiple groups. Conclusions identified previously undescribed that expand our understanding changes may impact immunopathology.

Language: Английский

The winding road: Infectious disease considerations for CAR-T and other novel adoptive cellular therapies in the era of COVID-19 DOI
Kanal Singh, Joseph M. Rocco, Véronique Nussenblatt

et al.

Seminars in Hematology, Journal Year: 2024, Volume and Issue: 61(5), P. 321 - 332

Published: Aug. 23, 2024

Language: Английский

Citations

2
Altered spike IgG Fc N-linked glycans are associated with hyperinflammatory state in adult COVID and Multisystem Inflammatory Syndrome in Children DOI
Jacob D. Sherman,

Vinit Karmali,

Bhoj Kumar

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 14, 2024

Abstract Background Severe COVID and multisystem inflammatory syndrome (MIS-C) are characterized by excessive cytokines/chemokines. In adults, disease severity is associated with SARS-CoV-2-specific IgG Fc afucosylation, which induces pro-inflammatory cytokine secretion from innate immune cells. This study aimed to define spike glycosylation following SARS-CoV-2 infection in adults children vaccination the relationships between glycan modifications cytokine/chemokine levels. Methods We analyzed longitudinal (n=146) cross-sectional (n=49) serum/plasma samples adult pediatric patients, MIS-C vaccinees, healthy controls. developed methods for characterizing bulk capillary electrophoresis (CE) measured levels of ten cytokines/chemokines multiplexed ELISA. Results Spike were more afucosylated than during acute MIS-C. observed an opposite trend vaccination, but it was not significant. galactosylated sialylated less bisected COVID, similar trends COVID/MIS-C vaccination. changed time or Afucosylated exhibited inverse positive correlations markers respectively; inversely correlated cytokines MIS-C; positively multiple groups. Conclusions identified previously undescribed that expand our understanding changes may impact immunopathology.

Language: Английский

Citations

0
Severe Acute Respiratory Syndrome Coronavirus 2 Plasma Antibody and Nucleocapsid Antigen Status Predict Outcomes in Outpatients With Coronavirus Disease 2019 DOI
Nikolaus Jilg, Mark J Giganti, Kara W. Chew

et al.

Clinical Infectious Diseases, Journal Year: 2024, Volume and Issue: 79(4), P. 920 - 927

Published: July 17, 2024

Reliable biomarkers of coronavirus disease 2019 (COVID-19) outcomes are critically needed. We evaluated associations spike antibody (Ab) and plasma nucleocapsid antigen (N Ag) with clinical in nonhospitalized persons mild-to-moderate COVID-19.

Language: Английский

Citations

0
Altered spike IgG Fc N-linked glycans are associated with hyperinflammatory state in adult COVID-19 and Multisystem Inflammatory Syndrome in Children DOI Creative Commons
Jacob D. Sherman,

Vinit Karmali,

Bhoj Kumar

et al.

Open Forum Infectious Diseases, Journal Year: 2024, Volume and Issue: 11(11)

Published: Oct. 16, 2024

Abstract Background Severe coronavirus disease 2019 (COVID-19) and multisystem inflammatory syndrome (MIS-C) are characterized by excessive cytokines/chemokines. In adults, severity is associated with severe acute respiratory 2 (SARS-CoV-2)–specific immunoglobulin G (IgG) Fc afucosylation, which induces proinflammatory cytokine secretion from innate immune cells. This study aimed to define spike IgG glycosylation following SARS-CoV-2 infection in adults children vaccination the relationships between glycan modifications Methods We analyzed longitudinal (n = 146) cross-sectional 49) serum/plasma samples adult pediatric COVID-19 patients, MIS-C vaccinees, controls. developed methods for characterizing bulk capillary electrophoresis measured levels of 10 cytokines/chemokines multiplexed enzyme-linked immunosorbent assay. Results Spike was more afucosylated than during MIS-C. observed an opposite trend vaccination, but it not significant. galactosylated sialylated less bisected COVID-19, similar trends COVID-19/MIS-C vaccination. changed time or Afucosylated exhibited inverse positive correlations markers respectively; inversely correlated cytokines MIS-C; positively multiple groups. Conclusions identified previously undescribed that expand our understanding changes may impact immunopathology.

Language: Английский

Citations

0