Intravital Multiphoton Microscopy of the Ocular Surface: Alterations in Conventional Dendritic Cell Morphology and Kinetics in Dry Eye Disease

Frontiers in Immunology, Год журнала: 2020, Номер 11

Опубликована: Май 7, 2020

Dry eye disease (DED) is a multifactorial of the ocular surface, characterized by loss tear film homeostasis and symptoms, in which neurosensory abnormalities have recently been shown to play an etiological role. Although role inflammation has widely studied DED, kinetics immune cells surface this complex are hereto unclear. Herein, we utilized intravital multi-photon imaging transgenic mice investigate 3D morphology conventional dendritic (cDCs), sensory nerves regulating them both naïve state experimental DED. DED had significantly lower secretion (p<0.01), greater corneal fluorescein staining (p<0.001), higher cDC density (p<0.05), compared mice. showed morphological alterations cDCs limbus, exhibiting smaller area (p<0.001) volume Further, sphericity (p<0.01). In addition, limbal displayed increased migratory including mean track speed, instantaneous velocity, length, displacement, (all p<0.05). with meandering index limbus central cornea (p<0.05). While contact demonstrated larger (p<0.05) as not mice, these differences were seen during Importantly, decreased velocity those Taken together, present vivo evidence altered apparent neuronal alters characteristics, indicating they may direct mediating response

Язык: Английский

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Dry eye symptoms in offices and deteriorated work performance – A perspective

Building and Environment, Год журнала: 2020, Номер 172, С. 106704 - 106704

Опубликована: Янв. 29, 2020

Irritated, stinging, tired, and dry eyes continue to be among top-two reported acute symptoms in modern office-like environments, despite use of new ventilation strategies low-emitting building materials. In this perspective, we disentangle why the prevalence external eye has remained stable without a noticeable decline over last decades. Furthermore, explore association between symptoms, perceived air deteriorated office work performance. The overall (as opposed internal) is caused by combination many environmental (climatic), occupational, personal risk factors, which alter stability precorneal tear film, essential for ocular surface health comfort. composition structure outer lipid layer film critical healthy surface. Identified major factors are apart from age gender, diseases (gland dysfunctions), particle (combustion) exposure, (e.g. climatic condition), occupational (visual demanding work) medication) conditions that aggravate stability, increases its vulnerability aggressive pollutants. It is, however, salient recognize (mild) (generally non-diagnosed) contribute may further overlap with both internal causalities. Thus, maintenance relief symptoms. Studies indicate indoor air, quality complaint, part induced low humidity, negatively affects Visual complaints like generally associated fatigue as workers, feel during typical intensive vision work, self-report lower performance symptoms; exacerbate workers elderly female workers.

Язык: Английский

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Inhibition of matrix metalloproteinase-9 for the treatment of dry eye syndrome; a review study

Experimental Eye Research, Год журнала: 2021, Номер 205, С. 108523 - 108523

Опубликована: Март 1, 2021

Язык: Английский

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Animal models of dry eye disease: Useful, varied and evolving (Review)

Experimental and Therapeutic Medicine, Год журнала: 2021, Номер 22(6)

Опубликована: Окт. 1, 2021

Dry eye disease (DED), which is a prevalent that still lacks successful treatment options, remains major challenge in ophthalmology. Multiple animal models of DED have been used to decipher its pathophysiology and develop novel treatments. These use mice, rats, rabbits, cats, dogs non‑human primates. Each model assesses aspects by focusing on elements the lacrimal functional unit, controls homeostasis tear film. The present review outlines representative their contribution study DED. Murine are most extensively used, followed rabbit models; latter offer advantage larger eyes, favorable biochemical profile for drug studies, experimental ease relatively low cost, contrasting with primates, which, although closer humans, not as accessible expensive. No comprehensive ‘ideal’ encompassing all human exists nor it feasible. Investigators often choose an based needs following four features given model: size eye, composition, available research reagents cost. As efforts expand, more refined needed supplement enormous made date existing models.

Язык: Английский

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Association between aging-dependent gut microbiome dysbiosis and dry eye severity in C57BL/6 male mouse model: a pilot study

BMC Microbiology, Год журнала: 2021, Номер 21(1)

Опубликована: Апрель 9, 2021

Abstract Background While aging is a potent risk factor of dry eye disease, age-related gut dysbiosis associated with inflammation and chronic geriatric diseases. Emerging evidence have demonstrated that contributes to the pathophysiology or exacerbation ocular diseases including disease. However, relationship between aging-related changes in microbiota disease has not been elucidated. In this pilot study, we investigated association aging-dependent microbiome severity C57BL/6 male mice. Results Eight-week-old (8 W, n = 15), one-year-old (1Y, 10), two-year-old (2Y, 8) mice were used. Dry was assessed by corneal staining scores tear secretion. Bacterial genomic 16 s rRNA from feces analyzed. Main outcomes compositional differences among groups their correlation severity. aged (1Y 2Y), increased secretion decreased statistical significance. Gut α-diversity different groups. β-diversity significantly univariate analysis, phylum Firmicutes , Proteobacteria, Cyanobacteria / Bacteroidetes ratio, genus Alistipes Bacteroides Prevotella Paraprevotella Helicobacter related After adjustment age, multivariate analysis revealed Proteobacteria Lactobacillus be Conclusions Our study suggests composition are signs

Язык: Английский

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