Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 105 - 137
Опубликована: Янв. 1, 2025
Язык: Английский
International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(1), С. 440 - 440
Опубликована: Янв. 4, 2021
Neurodegenerative diseases (ND) remains to be one of the biggest burdens on healthcare systems and serves as a leading cause disability death. Alzheimer’s disease (AD) is among most common such disorders, followed by Parkinson’s (PD). The basic molecular details initiation pathology are still under research. Only recently, role exosomes has been linked progression these neurodegenerative diseases. Exosomes small bilipid layer enclosed extracellular vesicles, which were once considered cellular waste functionless. These nano-vesicles 30–150 nm in diameter carry specific proteins, lipids, functional mRNAs, high amounts non-coding RNAs (miRNAs, lncRNAs, circRNAs). As content known vary per their originating recipient cells, vesicles can utilized diagnostic biomarker for early detection. Here we review exosomes, biogenesis, composition, We have also provided characterization through an array available techniques. Their updated discussed. Finally, shed light novel field salivary potential candidate diagnosis compared biomarkers with other blood/cerebrospinal fluid (CSF) based within neurological ailments.
Язык: Английский
Процитировано
128
Frontiers in Neuroscience, Год журнала: 2021, Номер 15
Опубликована: Авг. 31, 2021
Intercellular communication in the central nervous system (CNS) is essential for brain growth, development, and homeostasis maintenance and, when dysfunctional, involved occurrence development of neurodegenerative diseases. Increasing evidence indicates that extracellular vesicles, especially exosomes, are critical mediators intercellular signal transduction. Under physiological pathological conditions, neural cells secret exosomes with influence many factors. These can carry specific proteins, lipids, nucleic acids, other bioactive substances to recipient regulate their function. Depending on CNS environment, as well origin or status parental cells, mediate a variety different effects, including synaptic plasticity, nutritional metabolic support, nerve regeneration, inflammatory response, anti-stress effect, cellular waste disposal, propagation toxic components, playing an important role health This review will discuss possible roles both physiologic conditions.
Язык: Английский
Процитировано
84
Molecular Neurodegeneration, Год журнала: 2022, Номер 17(1)
Опубликована: Янв. 24, 2022
Abstract Amyotrophic Lateral Sclerosis (ALS) is a devastating neurodegenerative disease in which many processes are detected including (neuro)inflammation. Many drugs have been tested for ALS clinical trials but most failed to reach their primary endpoints. The development and inclusion of different types biomarkers diagnosis can assist determining target engagement drug, distinguishing between other diseases, predicting progression rate, drug responsiveness, or an adverse event. Ideally, among characteristics, biomarker correlates highly with process the central nervous system conveniently obtained peripheral tissue. Here, we describe state inflammation by focusing on peripherally detectable cellular responses from blood cells, provide new (combinatorial) directions exploration that now feasible due technological advancements.
Язык: Английский
Процитировано
51
International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(9), С. 4718 - 4718
Опубликована: Апрель 25, 2022
MicroRNAs (miRNAs) are essential post-transcriptional gene regulators involved in various neuronal and non-neuronal cell functions play a key role pathological conditions. Numerous studies have demonstrated that miRNAs dysregulated major neurodegenerative diseases, such as Alzheimer’s disease, Parkinson’s multiple sclerosis, amyotrophic lateral or Huntington’s disease. Hence, the present work, we constructed comprehensive overview of individual microRNA alterations models above diseases. We also provided evidence promising biomarkers for prognostic diagnostic approaches. In addition, summarized data from literature about miRNA-based therapeutic applications via inhibiting promoting miRNA expression. finally identified overlapping signature across including miR-128, miR-140-5p, miR-206, miR-326, miR-155, associated with etiological cellular mechanisms. However, it remains to be established whether what extent therapies could safely exploited future effective symptomatic disease-modifying approaches different human disorders.
Язык: Английский
Процитировано
51
Translational Neurodegeneration, Год журнала: 2022, Номер 11(1)
Опубликована: Дек. 12, 2022
Abstract Extracellular vesicles (EVs) are small bilipid layer-enclosed that can be secreted by all tested types of brain cells. Being a key intercellular communicator, EVs have emerged as contributor to the pathogenesis various neurodegenerative diseases (NDs) including Alzheimer’s disease, Parkinson’s amyotrophic lateral sclerosis, and Huntington’s disease through delivery bioactive cargos within central nervous system (CNS). Importantly, CNS cell-derived purified via immunoprecipitation, EV with altered levels been identified potential biomarkers for diagnosis prognosis NDs. Given essential impact on NDs, pathological considered therapeutic targets effects utilized agents or drug platforms treatment In this review, we focus recent research progress roles released from cells in summarize findings identify CNS-derived diagnose comprehensively discuss promising targets, agents, systems treating together current concerns challenges basic clinical applications regarding
Язык: Английский
Процитировано
49
Journal of Personalized Medicine, Год журнала: 2022, Номер 12(5), С. 770 - 770
Опубликована: Май 10, 2022
Alzheimer’s disease (AD), Parkinson’s (PD), and Amyotrophic Lateral Sclerosis (ALS) are representative neurodegenerative diseases (NDs) characterized by degeneration of selective neurons, as well the lack effective biomarkers therapeutic treatments. In last decade, microRNAs (miRNAs) have gained considerable interest in diagnostics therapy NDs, owing to their aberrant expression ability target multiple molecules pathways. Here, we provide an overview dysregulated miRNAs fluids (blood or cerebrospinal fluid) nervous tissue AD, PD, ALS patients. By emphasizing those that commonly these highlight potential role therapeutical targets describe use antisense oligonucleotides miRNA therapies.
Язык: Английский
Процитировано
46
Genes, Год журнала: 2023, Номер 14(2), С. 325 - 325
Опубликована: Янв. 27, 2023
Amyotrophic lateral sclerosis (ALS) is described as a fatal and rapidly progressive neurodegenerative disorder caused by the degeneration of upper motor neurons in primary cortex lower brainstem spinal cord. Due to ALS’s slowly characteristic, which often accompanied other neurological comorbidities, its diagnosis remains challenging. Perturbations vesicle-mediated transport autophagy well cell-autonomous disease initiation glutamatergic have been revealed ALS. The use extracellular vesicles (EVs) may be key accessing pathologically relevant tissues for ALS, EVs can cross blood–brain barrier isolated from blood. number content provide indications pathogenesis, stage, prognosis. In this review, we collected recent study aiming at identification biomarker ALS with respect size, quantity, biological fluids patients compared controls.
Язык: Английский
Процитировано
22
Translational Neurodegeneration, Год журнала: 2024, Номер 13(1)
Опубликована: Июнь 19, 2024
Abstract The central nervous system (CNS) is integrated by glial and neuronal cells, both release extracellular vesicles (EVs) that participate in CNS homeostasis. EVs could be one of the best candidates to operate as nanosized biological platforms for analysing multidimensional bioactive cargos, which are protected during systemic circulation EVs. Having a window into molecular level processes happening open new avenue research. This raises particular point interest: can CNS-derived blood serve circulating biomarkers reflect pathological status neurological diseases? L1 cell adhesion molecule (L1CAM) widely reported biomarker identify peripheral blood. However, it has been demonstrated L1CAM also expressed outside CNS. Given principal data related neurodegenerative diseases, such multiple sclerosis, amyotrophic lateral Parkinson’s disease Alzheimer’s were obtained using L1CAM-positive EVs, efforts overcome present challenges its specificity required. In this sense, other surface glutamate aspartate transporter (GLAST) myelin oligodendrocyte glycoprotein (MOG), among others, have started used. Establishing panel EV analyse increase sensitivity necessary these types studies. review covers main evidence cerebrospinal fluid samples patients with focusing on technical possibilities their isolation. emerging mirror brain physiopathology, reflecting localized changes. Therefore, when hindrances research clinical applications overcome, novel disease-specific panels would discovered facilitate transformation from traditional medicine personalized medicine.
Язык: Английский
Процитировано
11
Diagnostic Pathology, Год журнала: 2024, Номер 19(1)
Опубликована: Март 1, 2024
Abstract Background Amyotrophic lateral sclerosis (ALS) is a progressive and fatal motor neuron disease. Due to the limited knowledge about potential biomarkers that help in early diagnosis monitoring disease progression, today’s diagnoses are based on ruling out other diseases, neurography, electromyography examination, which takes time-consuming procedure. Methods PubMed, ScienceDirect, Web of Science were explored extract articles published from January 2015 June 2023. In searching strategy following keywords included; amyotrophic sclerosis, biomarkers, cerebrospinal fluid, serum, plama. Results A total number 6 studies describing fluid-based exosomal included this study. Aggregated proteins including SOD1, TDP-43, pTDP-43, FUS could be detected microvesicles (MVs). Moreover, TDP-43 NFL extracted plasma exosomes used as prognostic biomarkers. Also, downregulated miR-27a-3p through exoEasy Maxi exoQuick Kit measured diagnostic biomarker. Eventually, upregulated level CORO1A monitor progression. Conclusion Based results, each biomarker alone insufficient evaluate ALS. CNS-derived contain multiple ALS-related (SOD1, FUS, miRNAs) detectable fluid blood proper alternation. Exosome detecting kits listed exoEasy, ExoQuick, Exo-spin, ME kit, ExoQuick Plus, Exo-Flow, helpful reach purpose.
Язык: Английский
Процитировано
9
Cellular and Molecular Life Sciences, Год журнала: 2020, Номер 78(2), С. 561 - 572
Опубликована: Авг. 16, 2020
Abstract Extracellular vesicles (EVs) are small reservoirs of different molecules and important mediators cell-to-cell communication. As putative vehicles misfolded protein propagation between cells, they have drawn substantial attention in the field amyotrophic lateral sclerosis (ALS) other neurodegenerative disorders. Moreover, exosome-mediated non-coding RNA delivery may play a crucial role ALS, given relevance homeostasis disease pathogenesis. Since EVs can enter systemic circulation easily detectable patients’ biological fluids, generated broad interest both as diagnostic prognostic biomarkers valuable tools understanding Here, after brief introduction on biogenesis functions EVs, we aim to investigate their disorders, especially ALS. Specifically, focus main findings supporting EV-mediated transmission ALS vitro vivo models. Then, provide an overview clinical applications summarizing most relevant studies able detect blood cerebrospinal fluid (CSF) patients, underlying potential use aiding diagnosis prognosis. Finally, explore therapeutic either targets or proteins, nucleic acids molecular drugs.
Язык: Английский
Процитировано
57