Xanthene, cyanine, oxazine and BODIPY: the four pillars of the fluorophore empire for super-resolution bioimaging

Chemical Society Reviews, Год журнала: 2023, Номер 52(20), С. 7197 - 7261

Опубликована: Янв. 1, 2023

In the realm of biological research, invention super-resolution microscopy (SRM) has enabled visualization ultrafine sub-cellular structures and their functions in live cells at nano-scale level, beyond diffraction limit, which opened up a new window for advanced biomedical studies to unravel complex unknown details physiological disorders level with unprecedented resolution clarity. However, most SRM techniques are highly reliant on personalized special photophysical features fluorophores. recent times, there been an surge development robust fluorophore systems various imaging techniques. To date, xanthene, cyanine, oxazine BODIPY cores have authoritatively utilized as basic units small-molecule-based organic fluorescent probe designing strategies owing excellent characteristics easy synthetic acquiescence. Since future next-generation will be decided by availability probes these four building blocks play important role progressive design, is urgent need review advancements fluorophores different methods based dye cores. This article not only includes comprehensive discussion about developments emphasis effective integration into cell bio-imaging applications but also critically evaluates background each highlight merits demerits towards developing newer SRM.

Язык: Английский

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Actin Bundles Dynamics and Architecture

Biomolecules, Год журнала: 2023, Номер 13(3), С. 450 - 450

Опубликована: Фев. 28, 2023

Cells use the actin cytoskeleton for many of their functions, including division, adhesion, mechanosensing, endo- and phagocytosis, migration, invasion. Actin bundles are main constituent actin-rich structures involved in these processes. An ever-increasing number proteins that crosslink into or regulate morphology is being identified cells. With recent advances high-resolution microscopy imaging techniques, complex process formation multiple forms physiological beginning to be better understood. Here, we review physiochemical biological properties four families highly conserved abundant actin-bundling proteins, namely, α-actinin, fimbrin/plastin, fascin, espin. We describe similarities differences between role bundles, properties—both related unrelated bundling abilities. also some aspects general mechanism formation, which known from available information on activity key partners this process.

Язык: Английский

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Nanocarriers for cancer-targeted delivery based on Pickering emulsions stabilized by casein nanoparticles

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 140822 - 140822

Опубликована: Фев. 1, 2025

Язык: Английский

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ExPOSE: a comprehensive toolkit to perform expansion microscopy in plant protoplast systems

The Plant Journal, Год журнала: 2025, Номер 121(5)

Опубликована: Март 1, 2025

Expansion microscopy (ExM) achieves nanoscale imaging by physical expansion of fixed biological tissues embedded in a swellable hydrogel, enhancing the resolution any optical microscope several-fold. While ExM is commonly used animal cells and tissues, there are few plant-specific protocols. Protoplasts widely cell system across plant species, especially studying biomolecule localization. Here, we present an approach to achieve robust protoplasts, termed PrOtoplast SystEms (ExPOSE). We demonstrate that coupling ExPOSE with other techniques, immunofluorescence situ hybridization chain reaction visualize proteins mRNAs, respectively, greatly enhances spatial endogenous biomolecules. Additionally, this study, tested effectiveness versatility technique observe biomolecular condensates Arabidopsis protoplasts transcription factors maize at increased resolution. can be relatively inexpensive, fast, simple implement.

Язык: Английский

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Cellular insights into legume root infection by rhizobia

Current Opinion in Plant Biology, Год журнала: 2024, Номер 81, С. 102597 - 102597

Опубликована: Июль 27, 2024

Legume plants establish an endosymbiosis with nitrogen-fixing rhizobia bacteria, which are taken up from the environment anew by each host generation. This requires a dedicated genetic program on side to control microbe invasion, involving coordinated reprogramming of cells create infection structures that facilitate inward movement symbiont. Infection initiates in epidermis, different legumes utilizing distinct strategies for crossing this cell layer, either between (intercellular infection) or transcellularly (infection thread infection). Recent discoveries plant using fluorescent-based imaging approaches have illuminated spatiotemporal dynamics infection, underscoring importance investigating process at dynamic single-cell level. Extending fluorescence-based live-dynamic bacterial partner opens exciting prospect learning how individual reprogram rhizospheric host-confined state during early root infection.

Язык: Английский

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Unravelling the Mystery inside Cells by Using Single-Molecule Fluorescence Imaging

Journal of Imaging, Год журнала: 2023, Номер 9(9), С. 192 - 192

Опубликована: Сен. 19, 2023

Live-cell imaging is a powerful technique to study the dynamics and mechanics of various biological molecules like proteins, organelles, DNA, RNA. With rapid evolution optical microscopy, our understanding how these are implicated in cells’ most critical physiological roles deepens. In this review, we focus on spatiotemporal nanoscale live-cell at single molecule level allows for profound contributions towards new discoveries life science. This review will start by summarizing single-molecule tracking has been used analyze membrane dynamics, receptor–ligand interactions, protein–protein inner- extra-cellular transport, gene expression/transcription, whole organelle tracking. We then move current authors trying improve overcome limitations offering ways labeling proteins interest, multi-channel/color detection, improvements time-lapse imaging, methods programs colocalization movement targets. later discuss can be beneficial tool medical diagnosis. Finally, wrap up with future perspectives total internal reflection microscopy.

Язык: Английский

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PyrAtes: Modular Organic Salts with Large Stokes Shifts for Fluo‐rescence Microscopy

Angewandte Chemie International Edition, Год журнала: 2024, Номер 63(19)

Опубликована: Апрель 3, 2024

Abstract The deployment of small‐molecule fluorescent agents plays an ever‐growing role in medicine and drug development. Herein, we complement the portfolio powerful fluorophores, reporting serendipitous discovery development a novel class with imidazo[1,2‐a]pyridinium triflate core, which term PyrAtes . These fluorophores are synthesized single step from readily available materials (>60 examples) display Stokes shifts as large 240 nm, while also reaching NIR−I emissions at λ max long 720 nm. Computational studies allow platform for prediction Em Furthermore, demonstrate compatibility these live cell imaging HEK293 cells, suggesting potent intracellular markers.

Язык: Английский

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Introduction to Fluorescence Microscopy

Springer series on fluorescence, Год журнала: 2022, Номер unknown, С. 141 - 200

Опубликована: Янв. 1, 2022

Язык: Английский

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A Versatile Synaptotagmin‐1 Nanobody Provides Perturbation‐Free Live Synaptic Imaging And Low Linkage‐Error in Super‐Resolution Microscopy

Small Methods, Год журнала: 2023, Номер 7(10)

Опубликована: Июль 8, 2023

Imaging of living synapses has relied for over two decades on the overexpression synaptic proteins fused to fluorescent reporters. This strategy alters stoichiometry components and ultimately affects synapse physiology. To overcome these limitations, here a nanobody is presented that binds calcium sensor synaptotagmin-1 (NbSyt1). functions as an intrabody (iNbSyt1) in neurons minimally invasive, leaving transmission almost unaffected, suggested by crystal structure NbSyt1 bound Synaptotagmin-1 physiological data. Its single-domain nature enables generation protein-based reporters, showcased measuring spatially localized presynaptic Ca2+ with NbSyt1- jGCaMP8 chimera. Moreover, small size makes it ideal various super-resolution imaging methods. Overall, versatile binder will enable cellular molecular neuroscience unprecedented precision across multiple spatiotemporal scales.

Язык: Английский

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Exploring the future of regenerative medicine: Unveiling the potential of optical microscopy for structural and functional imaging of stem cells

Journal of Biophotonics, Год журнала: 2024, Номер 17(1)

Опубликована: Янв. 1, 2024

Regenerative medicine, which utilizes stem cells for tissue and organ repair, holds immense promise in healthcare. A comprehensive understanding of cell characteristics is crucial to unlock their potential. This study explores the pivotal role optical microscopy advancing regenerative medicine as a potent tool research. Advanced techniques enable an in-depth examination behavior, morphology, functionality. The review encompasses current microscopy, elucidating its capabilities constraints imaging, while also shedding light on emerging technologies improved visualization. Optical complemented by like fluorescence multiphoton enhances our comprehension dynamics. introduction label-free imaging facilitates noninvasive, real-time monitoring without external dyes or markers. By pushing boundaries researchers reveal intricate cellular mechanisms underpinning processes, thereby more effective therapeutic strategies. not only outlines future but underscores both structural functional imaging.

Язык: Английский

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