The NamesforLife Abstracts, Год журнала: 2021, Номер unknown
Опубликована: Фев. 26, 2021
Applied Microbiology and Biotechnology, Год журнала: 2021, Номер 105(6), С. 2527 - 2539
Опубликована: Фев. 15, 2021
Abstract In mollusk aquaculture, a large number of Vibrio species are considered major pathogens. Conventional methods based on DNA amplification and sequencing used to accurately identify unsuitable for monitoring programs because they time-consuming expensive. The aim this study was, therefore, develop the MALDI-TOF MS method in order establish rapid identification technique panel species. We created EnviBase containing 120 main spectra projections (MSP) that potentially responsible diseases, comprising 25 species: V. aestuarianus , cortegadensis tapetis belonging Coralliilyticus Harveyi Mediterranei Orientalis clades. Each MSP was constructed by merger raw obtained from three different media generated collaborating laboratories increase diversity conditions thus obtain good robustness. Perfect discrimination with all even very closely related as europaeus bivalvicida . new library validated through blind test 100 strains performed our collaborators who direct transfer protein extraction methods. majority were successfully identified newly both protocol This documents first development freely accessible database exclusively devoted found marine environments, taking into account high genus. Key points • Development quickly affiliate Increase reactivity when faced associated diseases Validation routine diagnostic tool
Язык: Английский
Процитировано
32
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown
Опубликована: Фев. 17, 2025
Abstract Globally, Marteilia parasites have been associated with significant mass mortality events in populations of commercially important bivalve molluscs, frequently resulting large-scale fishery collapses and substantial socio-economic impacts. The Wash Estuary, UK, supports several fisheries, among these, common cockles Cerastoderma edule suffered unusually high mortalities since 2008. We investigate potential causes these mortalities, confirm infection cocosarum , strongly cockle moribundity, also confirming its presence archived samples collected 2009. Molecular light microscopy screening during 2021, including healthy (buried) moribund (weak, unable to bury) cockles, indicated prevalence M. (PCR incidence up 95%) contrast (up 42%), suggesting an association between moribundity infection. Analysis the full ribosomal RNA array identified consistently different nucleotides infections (denoted as genotype WE) those Wales BI). 83% could be WE 12% BI, both genotypes recovered from 5% infected animals. Histopathologically, infects gill, mantle connective tissues, identical observations infecting Welsh cockles. Ongoing raise concerns regarding sustainability this resource ecologically economically. Additional measures may required reduce spread pathogen, noting that distribution beyond is currently unknown.
Язык: Английский
Процитировано
0
Journal of Clinical Microbiology, Год журнала: 2023, Номер 61(2)
Опубликована: Янв. 31, 2023
Increased interest in farmed aquatic species, conservation measures, and microbial metabolic end-product utilization have translated into a need for awareness recognition of novel species revisions to bacterial taxonomy. Because this has largely been unmet, through 4-year literature review, we present lists revised (including members the phylum
Язык: Английский
Процитировано
10
Frontiers in Molecular Biosciences, Год журнала: 2021, Номер 8
Опубликована: Сен. 3, 2021
The hemolymph metabolome of Mytilus galloprovincialis injected with live Vibrio splendidus bacteria was analyzed by 1 H-NMR spectrometry. Changes in spectral profiles were already detected after mussel acclimation (3 days at 18 or 25 °C). A significant decrease succinic acid accompanied an increase most free amino acids, mytilitol, and, to a smaller degree, osmolytes. These metabolic changes are consistent effective osmoregulation, and the restart aerobic respiration functional anaerobiosis occurred during transport. injection mussels acclimated 18°C caused several sugars, unassigned chemical species, more pronounced 24 than 12 h postinjection. Correlation heatmaps indicated dynamic adjustments relevance protein turnover maintaining homeostasis response stressful stimuli. This study confirms NMR-based metabolomics as feasible analytical approach complementary other omics techniques investigation responses environmental challenges.
Язык: Английский
Процитировано
20
Journal of Invertebrate Pathology, Год журнала: 2023, Номер 201, С. 108021 - 108021
Опубликована: Ноя. 1, 2023
Язык: Английский
Процитировано
4
New Microbes and New Infections, Год журнала: 2022, Номер 48, С. 101024 - 101024
Опубликована: Июль 1, 2022
The availability of multiple gene sequences, and in particular full genome sequence data, for microbial strains has changed how taxonomists delineate subspecies belonging to the Archaea Bacteria. Well-defined phylogenetic lineages that share higher similarity values compared widely used species thresholds are often described as subspecies, despite clear evidence genetic isolation between them. These well-defined lineages, reflecting notable core represent more recently evolved, unique sui generis evolutionary units. Because they bear all hallmarks species, most contemporary likely their own right. Although there is considerable value defining intraspecies variation (e.g., pathovar, serovar symbiovar), discriminating properties such units mostly encoded on accessory subgenomic compartments. We therefore argue taxonomic category become irrelevant propose its use should be discontinued. This will minimize inconsistencies related subjective nature species-subspecies distinctions. Formal recognition biologically relevant within based information have practical significance fields clinical, industrial agricultural microbiology.
Язык: Английский
Процитировано
7
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Год журнала: 2021, Номер 71(10)
Опубликована: Окт. 25, 2021
A psychrotolerant facultative anaerobe, strain SKBGT, was isolated from the bottom sediments of cold mineral spring Buxichen (Buryatia, Russia). Gram-positive non-motile cocci with a diameter 1.75-2.5 µm were observed singly or in long chains. Cells grew temperature range ̶ 5-35 °C. Growth within pH 7.0-9.5, optimum growth at 7.6 and NaCl concentration 0-1.0 % (optimum 0.1 (w/v)). Strain SKBGT chemoorganoheterotroph that used sugars some organic acids as substrates. The predominant fatty cell walls С16:1ω9, С18:1ω9, С16 : 0. 16S rRNA gene sequence shared high similarity (>99 %) those type strains genus Trichococcus. Digital DNA-DNA hybridization (dDDH) average nucleotide identity (ANI) values between Trichococcus shcherbakoviae ArtT (=DSM 107162T=VKM B-3260T) 70.1 95.4 %, respectively. genomic DNA G+C content 47.1 mol%. Compared T. shcherbakoviae, new characterized by for (10 °C) significantly lower than DSM 107162T (20-30 °C). Based on phenotypic characteristics, isolate classified subsp. psychrophilus nov. is (=VKM B-3241Т=JCM 33326T).
Язык: Английский
Процитировано
8
Scientific Reports, Год журнала: 2021, Номер 11(1)
Опубликована: Ноя. 12, 2021
Abstract Shellfish, including the key species common cockle Cerastoderma edule, living and feeding in waters contaminated by infectious agents can accumulate them within their tissues. It is unknown if microbial pathogens microparasites subsequently be transmitted via concomitant predation to consumers, shorebirds. The objective of this study was assess associated with C. edule could detected seasonally faeces shorebirds that feed on physical environment (sediment) which reside, along Irish Celtic Seas. Two potentially pathogenic global groups, Vibrio Haplosporidia, were . Although Haplosporidia not bird nor sediment, identical strains splendidus faecal samples at sites where oystercatcher Haematopus ostralegus other waders observed cockles. spp. prevalence seasonal increased during warmer months, possibly due higher seawater temperatures promote replication bacteria. sediment showed an overall than samples, its detection remained consistently high through throughout seasons, further supports role as a reservoir. Our findings shed light fact all pathogen groups are from prey predator but bacteria such V. be. As most wading birds migratory, results also indicate potential for bacterium dispersed over greater geographic distances, will have consequences areas it may introduced.
Язык: Английский
Процитировано
8
Elsevier eBooks, Год журнала: 2024, Номер unknown, С. 143 - 162
Опубликована: Окт. 18, 2024
Процитировано
0
Aquaculture Reports, Год журнала: 2024, Номер 39, С. 102480 - 102480
Опубликована: Ноя. 5, 2024
Язык: Английский
Процитировано
0