Antiviral effect of cetylpyridinium chloride in mouthwash on SARS-CoV-2

Scientific Reports, Год журнала: 2022, Номер 12(1)

Опубликована: Авг. 18, 2022

Cetylpyridinium chloride (CPC), a quaternary ammonium compound, which is present in mouthwash, effective against bacteria, fungi, and enveloped viruses. This study was conducted to explore the antiviral effect of CPC on SARS-CoV-2. There are few reports wild-type SARS-CoV-2 at low concentrations such as 0.001%-0.005% (10-50 µg/mL). Interestingly, we found that suppressed infectivity human isolated strains (Wuhan, Alpha, Beta, Gamma) even saliva. Furthermore, demonstrated shows anti-SARS-CoV-2 effects without disrupting virus envelope, using sucrose density analysis electron microscopic examination. In conclusion, this provided experimental evidence may inhibit infection lower concentrations.

Язык: Английский

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Aerosol in the oral health-care setting: a misty topic

Clinical Oral Investigations, Год журнала: 2023, Номер 27(S1), С. 23 - 32

Опубликована: Май 10, 2023

Abstract Studies have shown that mouth and respiratory tract microorganisms can be transported in aerosol spatter. Due to aerosol-generating procedures, there are potentially various infection risks for patients those working health care, especially oral care. Dental contaminate not only the mucous membranes of health-care professional’s mouth, passages, eyes but also exposed surfaces materials environment. As such, preventing disease transmission within offices is important issue. Since start COVID-19 pandemic, an innumerable amount (mis)information advice on how stay safe prevent spread coronavirus has been published. What preventive measures taken counteract this, what we learned during pandemic? This review summarizes relevant literature addressed presence dispersal spatter as a concern It includes sources dental aerosol, their potential threats, strategies controlling mitigating impact. shows further research needed better understand develop effective them. Clinical relevance Using personal protective equipment, high-volume evacuation systems pre-procedural antimicrobial agents help reduce settings protect well-being workers patients.

Язык: Английский

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Effectiveness of mouthwashes on reducing SARS-CoV-2 viral load in oral cavity: a systematic review and meta-analysis

BMC Oral Health, Год журнала: 2023, Номер 23(1)

Опубликована: Июль 3, 2023

Abstract Background The risk of SARS-COV-2 transmission is relatively high during dental procedures. A study was conducted to investigate the effects mouthwashes on viral load reduction in oral cavity. Methods systematic search performed PubMed, EMBASE, Scopus, Web Science, and Cochrane library for relevant studies up 20 July, 2022. Randomized non-randomized clinical trial quasi-experimental evaluating patients with Covid-19 infection (patients) who used (intervention) compared same before using mouthwash (comparison) reducing or increasing cycle threshold (Ct) value (outcome) were searched according PICO components. Three independent reviewers literature screening data extraction. Modified Downs Black checklist quality assessment. meta-analysis a random model Revman 5.4.1software mean difference (MD) values. Results Of 1653 articles, 9 methodological included. indicated that 1% Povidone-iodine (PVP-I) an effective [MD 3.61 (95% confidence interval 1.03, 6.19)]. Cetylpyridinium chloride (CPC) 0.61 -1.03, 2.25)] Chlorhexidine gluconate (CHX) -0.04 95% (-1.20, 1.12)] not against SARS-COV-2. Conclusion Using containing PVP-I may be recommended cavity procedures, while evidence sufficient such CPC CHX-containing mouthwashes.

Язык: Английский

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Efficacy of different mouthwashes against COVID-19: A systematic review and network meta-analysis

Japanese Dental Science Review, Год журнала: 2023, Номер 59, С. 334 - 356

Опубликована: Окт. 11, 2023

To evaluate the effectiveness of antiseptic mouthwashes in reducing SARS-CoV-2 load clinically and vitro. A systematic electronic search (MEDLINE/Scopus/Cochrane) was conducted to identify prospective clinical vitro studies published between 2019 included 16 June 2023 assessing saliva or surrogates. Data were summarized tables a network meta-analysis performed for trials. Thirty-five (14 RCTs, 21 vitro) fulfilled inclusion criteria. The risk bias judged be high 2 7 studies. most commonly test product chlorhexidine alone combination with other active ingredients, followed by povidone-iodine, hydrogen peroxide cetylpyridinium chloride. Overall, descriptive analysis revealed decreasing salivary viral both Network demonstrated degree heterogeneity. Among these studies, only 0.20% associated significant Ct increase 5 min after rinsing compared non-active control (p = 0.027). from suggested antiviral efficacy used mouthwashes. Large well-balanced trials are needed best protocols.

Язык: Английский

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Supporting the Aspecific Physiological Defenses of Upper Airways against Emerging SARS-CoV-2 Variants

Pathogens, Год журнала: 2023, Номер 12(2), С. 211 - 211

Опубликована: Янв. 29, 2023

The rapid rollout of COVID-19 vaccines in 2021 sparked general optimism toward controlling the severe form disease, preventing hospitalizations and COVID-19-associated mortality, transmissibility SARS-CoV-2 infection [...]

Язык: Английский

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Preprocedural Mouthwashes for Reduction of SARS-CoV-2 Viral Load and Infectivity

Journal of Dental Research, Год журнала: 2022, Номер 101(12), С. 1421 - 1423

Опубликована: Июль 27, 2022

Язык: Английский

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Cetylpyridinium Chloride-Containing Mouthwashes Show Virucidal Activity against Herpes Simplex Virus Type 1

Viruses, Год журнала: 2023, Номер 15(7), С. 1433 - 1433

Опубликована: Июнь 25, 2023

The oral cavity is particularly susceptible to viral infections that are self-recovering in most cases. However, complications may appear severe cases and/or immunocompromised subjects. Cetylpyridinium chloride (CPC)-containing mouthwashes able decrease the infectivity of SARS-CoV-2 virus by disrupting integrity envelope. Here, we show CPC, as active ingredient contained commercialized, exerts significant antiviral activity against enveloped viruses, such HSV-1, but not non-enveloped HPV. CPC-containing have been used antiseptics for decades, and thus, they can represent a cost-effective measure limit infection spread viruses infecting cavity, aiding reducing transmission.

Язык: Английский

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Disinfection and decontamination in the context of SARS‐CoV‐2‐specific data

Journal of Medical Virology, Год журнала: 2022, Номер 94(10), С. 4654 - 4668

Опубликована: Июнь 27, 2022

Given the high transmissibility of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as witnessed early in disease 2019 (COVID-19) pandemic, concerns arose with existing methods for virus disinfection and decontamination. The need SARS-CoV-2-specific data stimulated considerable research this regard. Overall, SARS-CoV-2 is practically equally susceptible to approaches decontamination that have been previously found other human or animal coronaviruses. latter included techniques utilizing temperature modulation, pH extremes, irradiation, chemical treatments. These physicochemical are a necessary adjunct prevention strategies, given environmental patient surface ubiquity virus. Classic studies also allowed extrapolation eradication on mucosal surfaces by some means. Despite laboratory study, practical field assessments generally lacking be encouraged confirm correlation interventions viral infection prevention. Transparency constitution use any method essential furthering applications.

Язык: Английский

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Substantivity of mouth-rinse formulations containing cetylpyridinium chloride and O-cymen-5-ol: a randomized-crossover trial

BMC Oral Health, Год журнала: 2022, Номер 22(1)

Опубликована: Дек. 27, 2022

Abstract Background The efficacy of mouth-rinses strongly depends upon their substantivity. use natural and non-toxic products that avoid secondary effects is gaining interest in preventive dentistry. purpose this study was to evaluate the substantivity two formulations mouth-washing solutions based on cetylpyridinium (CPC) O-cymen-5-ol. Methods This a randomized, double-blind, crossover trial conducted at Faculty Medicine Health Sciences University Barcelona. Bacterial re-colonization followed by live/dead (SYTO TM 9 + propidium iodide) bacterial staining measured confocal laser scanning microscopy fluorometry. Unstimulated saliva samples were collected from 16 healthy individuals baseline then, 15 min, 30 min 1, 2, 3, 4 h after following mouth-rinses: (i) single, 1-min mouth-rinse with ml placebo (negative control); (ii) CPC (0.05%) ; (iii) O-cymen-5-ol (0.09%); (iv) (0.09%). Results Proportion dead bacteria significantly higher for all mouthrinses during first compared (CPC = 48.0 ± 13.9; 95% CI 40.98–56.99; p < 0.001, 79.8 21.0; 67.71–91.90; 0.05, 49.4 14; 0.001 fluorometry 54.8 23.0; 41.50–68.06; 76.3 17.1; 66.36–86.14; 47.4 11.9; 40.49–54.30; microscopy, respectively). Nevertheless, h, only one obtained significant values as quantification methods used (80.3 22.8; 67.15–93.50; 0.05 81.4 13.8; 73.45–89.43; 0.05). combined increased mouthrinse respect prepared either active alone. Conclusion synergistic interaction prolongs resulting formulation may be effective other antimicrobials, such triclosan or chlorhexidine, but without undesirable effects. Thus, mouthrinsing Combinations replace near future Triclosan Chlorhexidine—based mouthrinses.

Язык: Английский

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Efficacy of Mouth Rinses and Nasal Spray in the Inactivation of SARS-CoV-2: A Systematic Review and Meta-Analysis of In Vitro and In Vivo Studies

International Journal of Environmental Research and Public Health, Год журнала: 2022, Номер 19(19), С. 12148 - 12148

Опубликована: Сен. 25, 2022

Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) is a global and evolving pandemic associated with heavy health financial burdens. Considering the oral cavity as major reservoir for SARS-CoV-2, systematic review meta-analysis were conducted to assess efficacy of mouth rinses nasal sprays in reducing salivary viral load SARS-CoV-2. All vivo vitro studies that assessed virucidal against SARS-CoV-2 published English language from December 2019 April 2022 considered analyses. Special Medical Subject Headings terms used search Pubmed, Scopus, Embase Ovid, Web Science databases. The toxicological data reliability assessment tool (ToxRToool) was quality included studies. Thirty-three (11 22 vitro) deemed eligible inclusion this analysis. Results pooled showed povidone-iodine most efficacious intervention load, followed by chlorhexidine. mean difference 86% 72%, respectively. Similarly, highest log10 reduction value (LRV) vitro, cetylpyridinium chloride, (LRV = 2.938 (p < 0.0005) LRV 2.907 0.009), respectively). Povidone-iodine-based preparations favourable results loads both vitro. limited number patients vivo, further among larger cohorts are recommended.

Язык: Английский

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