Differential mucosal tropism and dissemination of classical and hypervirulent Klebsiella pneumoniae infection DOI Creative Commons
Teck‐Hui Teo,

Nurul N. Ayuni,

Michelle Yin

и другие.

iScience, Год журнала: 2024, Номер 27(2), С. 108875 - 108875

Опубликована: Янв. 11, 2024

Klebsiella pneumoniae (Kp) infection is an important healthcare concern. The ST258 classical (c)Kp strain dominant in hospital-acquired infections North America and Europe, while ST23 hypervirulent (hv)Kp prevails community-acquired Asia. This study aimed to develop symptomatic mucosal models mice that mirror natural humans gain a deeper understanding of Kp pathogenesis. We showed cKp replicates the nasal cavity instead lungs, this early event crucial for establishment chronic colonization cecum colon. In contrast, hvKp directly lungs lethal bacterial load, esophagus supported downstream transient ileum cecum. Here, we have developed vivo model illuminates how differences tropism are responsible virulence disease phenotype hvKp, providing basis further mechanistic study.

Язык: Английский

International Clones of High Risk of Acinetobacter Baumannii—Definitions, History, Properties and Perspectives DOI Creative Commons
Andrey Shelenkov, В. Г. Акимкин,

Yulia Mikhaylova

и другие.

Microorganisms, Год журнала: 2023, Номер 11(8), С. 2115 - 2115

Опубликована: Авг. 19, 2023

Acinetobacter baumannii is a Gram-negative coccobacillus with exceptional survival skills in an unfavorable environment and the ability to rapidly acquire antibiotic resistance, making it one of most successful hospital pathogens worldwide, representing serious threat public health. The global dissemination A. driven by several lineages named ‘international clones high risk’ (ICs), two which were first revealed 1970s. Epidemiological surveillance crucial tool for controlling spread this pathogen, currently increasingly involves whole genome sequencing. However, assignment particular isolate some IC based on its genomic sequence not always straightforward requires computational from researchers, while definitions found literature are sometimes controversial. In review, we will focus typing tools suitable determination, provide data easily determine MLST type (ST) intrinsic blaOXA-51-like gene variants, discuss history current nine known ICs, IC1-IC9, investigate representation ICs databases. cgMLST profiles, as well OXA-51-like presence provided all isolates available GenBank. possible emergence novel international clone, IC10, be discussed.

Язык: Английский

Процитировано

26
Antimicrobial resistance level and conjugation permissiveness shape plasmid distribution in clinical enterobacteria DOI Creative Commons
Aída Alonso-del Valle, Laura Toribio-Celestino, Anna Quirant

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2023, Номер 120(51)

Опубликована: Дек. 14, 2023

Conjugative plasmids play a key role in the dissemination of antimicrobial resistance (AMR) genes across bacterial pathogens. AMR are widespread clinical settings, but their distribution is not random, and certain associations between clones particularly successful. For example, globally spread carbapenem plasmid pOXA-48 can use wide range enterobacterial species as hosts, it usually associated with small number specific

Язык: Английский

Процитировано

26
Heptavalent O-Antigen Bioconjugate Vaccine Exhibiting Differential Functional Antibody Responses Against Diverse Klebsiella pneumoniae Isolates DOI
Paeton L. Wantuch, Cory J. Knoot,

Lloyd S. Robinson

и другие.

The Journal of Infectious Diseases, Год журнала: 2024, Номер 230(3), С. 578 - 589

Опубликована: Фев. 24, 2024

Abstract Klebsiella pneumoniae is the leading cause of neonatal sepsis and increasingly difficult to treat owing antibiotic resistance. Vaccination represents a tractable approach combat this resistant bacterium; however, there currently not licensed vaccine. Surface polysaccharides, including O-antigens lipopolysaccharide, have long been attractive candidates for vaccine inclusion. Herein we describe generation bioconjugate targeting 7 predominant O-antigen subtypes in K. pneumoniae. Each was immunogenic isolation, with limited cross-reactivity among subtypes. Vaccine-induced antibodies demonstrated varying degrees binding wide variety strains. Furthermore, serum from vaccinated mice induced complement-mediated killing many these Finally, increased capsule interfered ability bind mediate some Taken together, data indicate that novel heptavalent formulation exhibits efficacy against some, but all, isolates.

Язык: Английский

Процитировано

13
Exploring the journey: A comprehensive review of vaccine development against Klebsiella pneumoniae DOI
Bruno Douradinha

Microbiological Research, Год журнала: 2024, Номер 287, С. 127837 - 127837

Опубликована: Июль 18, 2024

Язык: Английский

Процитировано

13
Capsules and their traits shape phage susceptibility and plasmid conjugation efficiency DOI Creative Commons
Matthieu Haudiquet, Julie Le Bris, Amandine Nucci

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Март 6, 2024

Abstract Bacterial evolution is affected by mobile genetic elements like phages and conjugative plasmids, offering new adaptive traits while incurring fitness costs. Their infection the bacterial capsule. Yet, its importance has been difficult to quantify because of high diversity confounding mechanisms in genomes such as anti-viral systems surface receptor modifications. Swapping capsule loci between Klebsiella pneumoniae strains allowed us their impact on plasmid phage independently background. Capsule swaps systematically invert susceptibility, revealing serotypes key determinants infection. types also influence conjugation efficiency both donor recipient cells, a mechanism shaped volume pilus structure. Comparative genomics confirmed that more permissive lab correspond acquiring plasmids nature. The least capsule-sensitive pili (F-like) are most frequent species’ only ones associated with antibiotic resistance virulence factors, driving convergence antibiotics population. These results show how cellular envelopes define slow fast lanes elements, implications for population dynamics horizontal gene transfer.

Язык: Английский

Процитировано

12
Prediction of Klebsiella phage-host specificity at the strain level DOI Creative Commons
Dimitri Boeckaerts, Michiel Stock, Celia Ferriol-González

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Май 22, 2024

Abstract Phages are increasingly considered promising alternatives to target drug-resistant bacterial pathogens. However, their often-narrow host range can make it challenging find matching phages against bacteria of interest. Current computational tools do not accurately predict interactions at the strain level in a way that is relevant and properly evaluated for practical use. We present PhageHostLearn, machine learning system predicts strain-level between receptor-binding proteins receptors Klebsiella phage-bacteria pairs. evaluate this both silico laboratory, clinically setting finding strains. PhageHostLearn reaches cross-validated ROC AUC up 81.8% maintains performance laboratory validation. Our approach provides framework developing evaluating phage-host prediction methods useful practice, which we believe be meaningful contribution machine-learning-guided development phage therapeutics diagnostics.

Язык: Английский

Процитировано

12
Genomic surveillance as a scalable framework for precision phage therapy against antibiotic-resistant pathogens DOI Creative Commons
Mihály Koncz, Tamás Stirling,

Hiba Hadj Mehdi

и другие.

Cell, Год журнала: 2024, Номер unknown

Опубликована: Сен. 1, 2024

Язык: Английский

Процитировано

9
Genomic Analysis of Virulent, Multidrug Resistant Klebsiella pneumoniae and Klebsiella oxytoca from Bloodstream Infections, South Africa DOI Creative Commons
Bakoena A. Hetsa, Jonathan Asante, Joshua Mbanga

и другие.

Microbial Pathogenesis, Год журнала: 2025, Номер unknown, С. 107272 - 107272

Опубликована: Янв. 1, 2025

The study investigated the resistome, virulome and mobilome of multidrug resistant (MDR) Klebsiella pneumoniae oxytoca clinical isolates. A total 46 suspected species (spp.) were collected from blood cultures within uMgungundlovu District in KwaZulu-Natal Province. Antibiotic susceptibility was determined against a panel 19 antibiotics using disk diffusion test. subset 14 MDR K. (n=10) (n=4) isolates selected based on their antibiograms subjected to whole genome sequencing (WGS). sequence types (STs), virulome, mobilome, capsule loci (KLs) analysed relevant WGS bioinformatics tools. Of 10 (ST) identified, most common ST25 (n=3), ST101 4 belonged ST450 (n=3). two high-risk clones ST15, ST17 identified. O K with predominance KL2, KL17, KL29, O1/O2v2, O1/O2v1, OL104 respectively. majority displayed resistance predominantly carrying β-lactamase genes, including bla convergence hypervirulence genes strains is potential concern. Carbapenemase, ESBL screening genomic surveillance are urgently required hospital environments.

Язык: Английский

Процитировано

1
A panel of diverse Klebsiella pneumoniae clinical isolates for research and development DOI Creative Commons
Melissa J. Martin, William Stribling,

Ana C. Ong

и другие.

Microbial Genomics, Год журнала: 2023, Номер 9(5)

Опубликована: Май 4, 2023

are a leading cause of healthcare-associated infections worldwide. In particular, strains expressing extended-spectrum β-lactamases (ESBLs) and carbapenemases pose serious treatment challenges, the World Health Organization (WHO) to designate ESBL carbapenem-resistant Enterobacteriaceae as 'critical' threats human health. Research efforts combat these pathogens can be supported by accessibility diverse clinically relevant isolates for testing novel therapeutics. Here, we describe panel 100

Язык: Английский

Процитировано

20
Virulence factors in carbapenem-resistant hypervirulent Klebsiella pneumoniae DOI Creative Commons
Gabriel Mendes, Maria Leonor Santos, João F. Ramalho

и другие.

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Ноя. 30, 2023

Hypervirulence and carbapenem-resistant have emerged as two distinct evolutionary pathotypes of Klebsiella pneumoniae, with both reaching their epidemic success posing a great threat to public health. However, the boundaries separating these fade, we assist worrisome convergence in certain high-risk clones, causing hospital outbreaks challenging every therapeutic option available. To better understand basic biology pathogens, this review aimed describe virulence factors distribution worldwide among highly virulent or hypervirulent K. pneumoniae strains, well interplay strains carbapenemase produced sequence type such strains. As witness shift healthcare settings where are beginning emerge replace classical understanding is urgently needed for immediate appropriate response.

Язык: Английский

Процитировано

20