Genomic Epidemiology Dataset for the Important Nosocomial Pathogenic Bacterium Acinetobacter baumannii

Data, Год журнала: 2024, Номер 9(2), С. 22 - 22

Опубликована: Янв. 26, 2024

The infections caused by various bacterial pathogens both in clinical and community settings represent a significant threat to public healthcare worldwide. growing resistance antimicrobial drugs acquired species causing healthcare-associated has already become life-threatening danger noticed the World Health Organization. Several groups or lineages of isolates, usually called ‘the clones high risk’, often drive spread within particular species. Thus, it is vitally important reveal track such mechanisms which they acquire antibiotic enhance their survival skills. Currently, analysis whole-genome sequences for isolates interest increasingly used these purposes, including epidemiological surveillance development prevention measures. However, availability uniformity data derived from genomic bottleneck investigations. With this dataset, we present results epidemiology 17,546 genomes dangerous pathogen, Acinetobacter baumannii. Important typing information, multilocus sequence (MLST)-based types (STs), intrinsic blaOXA-51-like gene variants, capsular (KL) oligosaccharide (OCL) types, CRISPR-Cas systems, cgMLST profiles are presented, as well assignment nine known international risk. presence genes also reported. These will be useful researchers field A. baumannii epidemiology, analysis, measure development.

Язык: Английский

---

A panel of genotypically and phenotypically diverse clinical Acinetobacter baumannii strains for novel antibiotic development

Microbiology Spectrum, Год журнала: 2024, Номер 12(8)

Опубликована: Июнь 25, 2024

ABSTRACT Acinetobacter baumannii is one of the most important pathogens worldwide. The intrinsic and acquired resistance A. , coupled with slow pace novel antimicrobial drug development, poses an unprecedented enormous challenge to clinical anti-infective therapy . Recent studies in field pathogenicity, antibiotic resistance, biofilms have focused on model strains, including ATCC 17978, 19606, AB5075. However, these strains represent only a limited portion heterogeneity Furthermore, variants emerged that show significant diversity not at genotypic level but also reflected differences phenotypic levels capsule, virulence, resistance. Research key pathogen, would benefit from standardized approach, which characterizes heterogeneous order facilitate rapid diagnosis, discovery new therapeutic targets, efficacy assessment. Our study provides describes standardized, genomically phenotypically panel 45 different for research community. In addition, we performed comparative analyses several phenotypes this panel. We found sequence type 2 (ST2) group showed significantly higher rates lower fitness cost adaptation, yet less biofilm formation. Macrocolony E (MTE, flat center wavy edge phenotype reported literature) clear correlation growth rate, was observed produce more biofilms. sheds light complex interplay formation within distinct offering insights crucial combating infection. IMPORTANCE globally notorious, effort combat spread such pathogens, emerging candidate therapies already surfaced. used test vary across (the sources numbers are varied often very large, little heterogeneity). variation complicates studies. resources greatly restricted physiology, Therefore, it community acquire meticulously selected diverse total 2,197 isolates collected 64 hospitals 27 provinces China, providing scientific reference This assistance will exchange academic research.

Язык: Английский

---

Characterization of the carbapenem-resistant Acinetobacter baumannii clinical reference isolate BAL062 (CC2:KL58:OCL1): resistance properties and capsular polysaccharide structure

mSystems, Год журнала: 2024, Номер unknown

Опубликована: Сен. 10, 2024

ABSTRACT The carbapenem-resistant Acinetobacter baumannii isolate BAL062 is a clinical reference used in several recent experimental studies. It from ventilator-associated pneumonia (VAP) patient an intensive care unit at the Hospital for Tropical Diseases (HTD), Ho Chi Minh City, Vietnam 2009. Here, was found to belong B sub-lineage of global clone 2 (GC2) isolates previously reported outbreak (2008 and 2012) VAP A. HTD. While related were extensively antibiotic-resistant carry GC2-associated genomic resistance islands, AbGRI1, AbGRI2, AbGRI3, has lost AbGRI3 three aminoglycoside genes, armA, aacA4, aphA1 , leading amikacin, tobramycin kanamycin susceptibility. location Tn 2008 VAR chromosome this also corrected. Like many isolates, carries KL58 gene cluster capsular polysaccharide (CPS) synthesis locus annotation key provided. As information about K type important development novel CPS-targeting therapies, K58-type CPS structure established using NMR spectroscopy. most closely K2 K93, sharing similar configurations linkages between units, contains rare higher monosaccharide, 5,7-diacetamido-3,5,7,9-tetradeoxy- d - glycero l manno -non-2-ulosonic acid (5,7-di- N -acetyl-8-epipseudaminic acid; 8ePse5Ac7Ac), 8-epimer Pse5Ac7Ac -acetylpseudaminic acid). Inspection publicly available genomes revealed wide distribution geographically diverse belonging sequence types that recovered over two decades clinical, animal, environmental sources. IMPORTANCE Many published studies aimed developing clearer understanding pathogenicity strains currently causing treatment failure due extensive antibiotic are undertaken historic, laboratory-adapted isolates. However, it ideal if not imperative such characterized here belongs dominant GC2 resistant infections been various correlation profiles data identifying genes knockout complementation analyses, we have mapped find candidates. Novel as bacteriophage or monoclonal antibody under investigation alternatives adjuncts combat difficult-to-treat CRAb often exhibit specificity specific structural epitopes (CPS), outer-most layer. solved other consistent naming identification interpretation studies, correlated automatic annotations standard names.

Язык: Английский

---

Emergence ofAcinetobacter baumanniiInternational Clone 10 predominantly found in the Middle East

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Окт. 9, 2023

Abstract Acinetobacter baumannii is a globally distributed human pathogen. Infections caused by carbapenem-resistant isolates of A. (CRAB) are great concern, as treatment options very limited. Despite having among the highest rates reported worldwide, there exists limited genomic data from CRAB strains isolated in Middle East. Here we report epidemiological, phenotypic, and genome sequencing (short reads long reads) on set 60 belonging to Sequence Type ST158 (Pasteur MLST scheme). They represent novel international clone (IC), designated IC10, with geographic spread beyond Specific antibiotic-resistance genes associated this were identified plasmid content lineage presented.

Язык: Английский

---

Genomic Epidemiology Dataset for Important Nosocomial Pathogenic Bacteria <em>Acinetobacter baumannii</em>

Опубликована: Ноя. 29, 2023

The infections caused by various bacterial pathogens both in clinical and community settings represent a significant threat to public healthcare worldwide. growing resistance antimicrobial drugs acquired species causing healthcare-associated has already become life-threatening danger noticed the World Health Organization. Several groups or lineages of isolates usually called ‘the clones high risk’ often drive spread within particular species. Thus, it is vitally important reveal track such mechanisms which they acquire antibiotic enhance their survival skills. Currently, analysis whole genome sequences for interest increasingly used these purposes, including epidemiological surveillance developing prevention measures. However, availability uniformity data derived from genomic represents bottleneck investigations. In this dataset, we present results comprehensive epidemiology 17,546 genomes dangerous pathogen Acinetobacter baumannii. Important typing information multilocus sequence (MLST)-based types (STs), intrinsic blaOXA-51-like gene variants, capsular (KL) oligosaccharide (OCL) types, CRISPR-Cas systems, cgMLST profiles are presented, as well assignment nine known international risk. presence genes also reported. These will be useful researchers field A. baumannii epidemiology, measure development.

Язык: Английский

---

Phylogenomic and phenotypic analyses highlight the diversity of antibiotic resistance and virulence in both human and non-humanAcinetobacter baumannii

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Дек. 14, 2023

Abstract Acinetobacter baumannii is a Gram-negative opportunistic pathogen causing infections of the immunocompromised. With high incidence muti-drug resistance, carbapenem-resistant A. as priority 1 designated by WHO. The current literature has expertly characterized clinical isolates baumannii. As challenge these recently been classified One Health issue, we set out to explore diversity from human and non-clinical sources such agricultural surface water, urban streams, various effluents wastewater-treatment plants tank milk; and, importantly, came wide geographic distribution. Phylogenomic analysis considering almost 200 showed that our diverse well-differentiated main international clones . We discovered novel sequence types in both hospital settings, five strains overexpress RND efflux pump adeIJK without changes susceptibility. Further, detected bla ADC-79 non-human isolate despite its sensitivity all antibiotics. There was no significant differentiation between virulence profiles Galleria mellonella insect model suggesting neither dependent on origin nor isolation source. Detection antibiotic resistance genes suggests may act genetic reservoir for strains. This endorses notion order combat multi-drug resistant infection caused baumannii, approach required, deeper understanding must be achieved. Importance global crisis silent one. More more bacteria are becoming antibiotics available treatment, leaving options remaining. includes shows frequency many last-resort drugs carbapenem colistin. Research focused origin, but there knowledge gap regarding traits, particularly, how become notorious today. Antibiotic have animals, environmental locations grass soil. such, concern which health humans, animals environment. Thus, truly crisis, need understand gene reservoirs this under continuum. Repositories NCBI GenBank Accession numbers: Bioproject PRJNA819071, Biosamples SAMN26898552 - SAMN26898587.

Язык: Английский

---