Abstract
In
this
study,
we
use
integrated
transcriptomic
datasets
from
the
GEO
repository
with
purpose
of
investigating
immune
dysregulation
in
COVID-19.
Thus,
context,
decided
to
be
focused
on
NK
cells
and
CD14+
monocytes
gene
expression,
considering
GSE165461
GSE198256,
respectively.
Other
PBMCs,
lung,
olfactory,
sensory
epithelium
lymph
were
used
provide
robust
validation
for
our
results.
This
approach
gave
an
view
responses
COVID-19,
pointing
out
a
set
potential
biomarkers
therapeutic
targets
special
regard
standards
physiological
conditions.
IFI27,
MKI67,
CENPF,
MBP,
HBA2,
TMEM158,
THBD,
HBA1,
LHFPL2,
SLA,
AC104564.3
identified
as
key
genes
analysis
that
have
critical
biological
processes
related
inflammation,
regulation,
oxidative
stress,
metabolic
processes.
Consequently,
such
are
important
understanding
heterogeneous
clinical
manifestations
COVID-19—from
acute
long-term
effects
now
known
‘long
COVID’.
Subsequent
additional
consolidated
these
role
diagnosis
COVID-19
prediction
its
severity.
Moreover,
their
enrichment
pathophysiological
pathways
presented
them
intervention.The
results
insight
into
molecular
dynamics
caused
by
other
monocytes.
study
constitutes
solid
basis
targeted
diagnostic
development
makes
relevant
contributions
ongoing
research
efforts
toward
better
management
mitigation
pandemic.
Journal of the Pediatric Infectious Diseases Society,
Год журнала:
2023,
Номер
12(10), С. 540 - 548
Опубликована: Окт. 1, 2023
In
the
fall
of
2022,
we
observed
a
sharp
rise
in
pediatric
Invasive
Group
A
Streptococcus
(iGAS)
hospitalizations
Colorado.
We
compared
epidemiology,
clinical
features,
and
patient
outcomes
this
outbreak
to
prior
years.Between
October
2022
April
2023,
prospectively
identified
reviewed
iGAS
cases
hospitalized
patients
at
Children's
Hospital
Using
laboratory
specimen
records,
also
retrospectively
number
with
sterile
site
GAS-positive
cultures
across
three
time
periods:
pre-COVID-19
(January
2015-March
2020),
height
COVID-19
pandemic
(April
2020-September
2022),
(October
2022-April
2023).Among
96
cases,
median
age
was
5.7
years
old;
66%
were
male,
70%
previously
healthy,
39%
required
critical
care,
four
died.
Almost
60%
had
associated
respiratory
viral
symptoms,
10%
toxic
shock
syndrome,
4%
necrotizing
fasciitis.
Leukopenia,
bandemia,
higher
C-reactive
protein
values
findings
need
for
care.
There
significantly
more
during
(9.9/month
vs
3.9/month
pre-pandemic
1.3/month
pandemic),
including
pneumonia
(28%
15%
0%
pandemic)
multifocal
disease
(17%
3%
P
<
.001
all.Outbreak
case
numbers
almost
triple
baseline.
The
high
percentage
symptoms
suggests
link
coinciding
surges
viruses
time.
GAS
can
be
severe
evolve
rapidly;
features
may
help
earlier
identification
critically
ill
children.
medRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Фев. 13, 2024
Abstract
During
the
COVID-19
pandemic
it
was
widely
described
that
certain
individuals
infected
by
SARS-CoV-2
experience
persistent
disease
signs
and
symptoms,
Long
COVID,
which
in
some
cases
is
very
severe
with
life
changing
consequences.
To
maximize
our
chances
of
identifying
underpinnings
this
illness,
we
have
focused
on
121
most
from
>1000
patients
screened
specialized
clinics
Sweden
Belgium.
We
restricted
study
to
subjects
objective
measures
organ
damage
or
dysfunction,
>3
months
following
a
verified,
but
mild-to-moderate
infection.
By
performing
systems-level
immunological
testing
comparisons
controls
fully
convalescent
similar
mild/moderate
episode,
identify
elevated
serological
responses
COVID
suggestive
chronic
antigen
stimulation.
Persistent
viral
reservoirs
been
proposed
using
multiple
orthogonal
methods
for
detection
RNA
protein
plasma
subset
detectable
antigens,
minimal
overlap
across
assays,
no
correlation
symptoms
immune
measurements.
Elevated
serologic
other
hand
were
inversely
correlated
clonally
expanded
memory
CD8
+
T
cells,
indicating
restrained
clonal
expansion
enables
persistence,
exposure
IgG
responses,
even
if
antigen-detection
blood
not
universally
possible.
Computers in Biology and Medicine,
Год журнала:
2024,
Номер
175, С. 108507 - 108507
Опубликована: Апрель 22, 2024
Post
COVID-19
Condition
(PCC),
characterized
by
lingering
symptoms
post-acute
COVID-19,
poses
clinical
challenges,
highlighting
the
need
to
understand
its
underlying
molecular
mechanisms.
This
meta-analysis
aims
shed
light
on
transcriptomic
landscapes
and
sex-specific
dynamics
intrinsic
PCC.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(24), С. 17468 - 17468
Опубликована: Дек. 14, 2023
The
presence
of
long
COVID
(LC)
following
SARS-CoV-2
infection
is
a
common
condition
that
affects
the
quality
life
patients
and
represents
diagnostic
challenge
due
to
diversity
symptoms
may
coexist.
We
still
do
not
have
accurate
information
regarding
pathophysiological
pathways
generate
LC,
so
it
important
know
inflammatory
immunothrombotic
biomarker
profiles
their
implications
in
order
characterize
risk
subgroups
establish
early
therapeutic
strategies.
performed
determination
biomarkers
volunteers
with
previous
diagnoses
SARS-CoV-2.
were
analyzed
plasma
by
flow
cytometry,
we
von
Willebrand
factor
(vWF)
samples
using
ELISA.
clinical
variables
or
absence
then
analyzed.
IL-6,
sCD40L,
p-Selectin,
PSGL-1,
PAI-1,
tPA,
D-Dimer,
TF,
Factor
IX
levels
elevated
groups
especially
subgroup
metabolic
syndrome
(MetS).
VWF
found
be
increased
sequelae
MetS.
Our
results
confirmed
persistence
an
active
state,
identify
population
at
provide
adequate
follow-up.
Journal of Cellular Biochemistry,
Год журнала:
2023,
Номер
125(1)
Опубликована: Дек. 14, 2023
Abstract
Considerable
research
has
been
done
in
investigating
SARS‐CoV‐2
infection,
its
characteristics,
and
host
immune
response.
However,
debate
is
still
ongoing
over
the
emergence
of
post‐acute
sequelae
infection
(PASC).
A
multitude
long‐lasting
symptoms
have
reported
several
weeks
after
primary
acute
that
resemble
other
viral
infections.
Thousands
articles
described
various
post‐COVID‐19
conditions.
Yet,
evidence
around
these
health
problems,
reasons
behind
them,
their
molecular
underpinnings
are
scarce.
These
persistent
also
known
as
long
COVID‐19.
The
persistence
and/or
components
tissues
can
lead
to
COVID.
For
example,
presence
nucleocapsid
protein
RNA
was
detected
skin,
appendix,
breast
some
COVID
patients.
multiple
anatomic
sites,
including
non‐respiratory
such
adrenal
gland,
ocular
tissue,
small
intestine,
lymph
nodes,
myocardium,
sciatic
nerve.
Distinctive
spike
sequence
variants
were
found
tissues.
Interestingly,
prolonged
detection
subgenomic
observed
across
all
tissues,
sometimes
same
patient,
which
likely
reflects
recent
but
defective
replication.
Moreover,
noticed
throughout
brain
at
autopsy,
late
230
days
following
symptom
onset
among
unvaccinated
patients
who
died
severe
infection.
Here,
we
review
an
intrinsic
factor
We
highlight
immunological
consequences
this
persistence.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 12, 2024
ABSTRACT
Globally,
over
65
million
individuals
are
estimated
to
suffer
from
post-acute
sequelae
of
COVID-19
(PASC).
A
large
number
living
with
PASC
experience
cardiovascular
symptoms
(i.e.
chest
pain
and
heart
palpitations)
(PASC-CVS).
The
role
chronic
inflammation
in
these
symptoms,
particular
persisting
for
>1
year
after
SARS-CoV-2
infection,
remains
be
clearly
defined.
In
this
cross-sectional
study,
blood
samples
were
obtained
three
different
sites
Australia
i)
a
resolved
infection
(and
no
persistent
i.e.
‘Recovered’),
ii)
prolonged
PASC-CVS
iii)
negative
individuals.
Individuals
PASC-CVS,
relative
Recovered
individuals,
had
transcriptomic
signature
associated
inflammation.
This
was
accompanied
by
elevated
levels
pro-inflammatory
cytokines
(IL-12,
IL-1β,
MCP-1
IL-6)
at
approximately
18
months
post-infection.
These
present
trace
amounts,
such
that
they
could
only
detected
the
use
novel
nanotechnology.
Importantly,
trace-level
direct
effect
on
functionality
pluripotent
stem
cell
derived
cardiomyocytes
vitro
.
not
observed
presence
dexamethasone.
Plasma
proteomics
demonstrated
further
differences
between
patients
post-infection
including
enrichment
complement
coagulation
proteins
those
symptoms.
Together,
data
provide
new
insight
into
nanotechnology
as
possible
diagnostic
approach
condition.
