Integrative Transcriptomic Profiling of NK Cells and Monocytes: Advancing Diagnostic and Therapeutic Strategies for COVID-19. DOI Creative Commons

Salma LOUKMAN,

Reda Ben Mrid,

Najat Bouchmaa

et al.

Published: Oct. 21, 2024

Abstract In this study, we use integrated transcriptomic datasets from the GEO repository with purpose of investigating immune dysregulation in COVID-19. Thus, context, decided to be focused on NK cells and CD14+ monocytes gene expression, considering GSE165461 GSE198256, respectively. Other PBMCs, lung, olfactory, sensory epithelium lymph were used provide robust validation for our results. This approach gave an view responses COVID-19, pointing out a set potential biomarkers therapeutic targets special regard standards physiological conditions. IFI27, MKI67, CENPF, MBP, HBA2, TMEM158, THBD, HBA1, LHFPL2, SLA, AC104564.3 identified as key genes analysis that have critical biological processes related inflammation, regulation, oxidative stress, metabolic processes. Consequently, such are important understanding heterogeneous clinical manifestations COVID-19—from acute long-term effects now known ‘long COVID’. Subsequent additional consolidated these role diagnosis COVID-19 prediction its severity. Moreover, their enrichment pathophysiological pathways presented them intervention.The results insight into molecular dynamics caused by other monocytes. study constitutes solid basis targeted diagnostic development makes relevant contributions ongoing research efforts toward better management mitigation pandemic.

Language: Английский

Immunobiology of COVID-19: Mechanistic and therapeutic insights from animal models DOI Open Access

Hong‐Yi Zheng,

Tian‐Zhang Song,

Yong‐Tang Zheng

et al.

动物学研究, Journal Year: 2024, Volume and Issue: 45(4), P. 747 - 766

Published: Jan. 1, 2024

The distribution of the immune system throughout body complicates

Language: Английский

Citations

4
A single-cell RNA sequencing dataset of peripheral blood cells in long COVID patients on herbal therapy DOI Creative Commons
Karolina Hanna Prazanowska, Tae-Hun Kim, Jung Won Kang

et al.

Scientific Data, Journal Year: 2025, Volume and Issue: 12(1)

Published: Jan. 30, 2025

Language: Английский

Citations

0
Outbreak of Invasive Group A Streptococcus in Children—Colorado, October 2022–April 2023 DOI
Erin C Ho, Jessica R. Cataldi, Lori Silveira

et al.

Journal of the Pediatric Infectious Diseases Society, Journal Year: 2023, Volume and Issue: 12(10), P. 540 - 548

Published: Oct. 1, 2023

In the fall of 2022, we observed a sharp rise in pediatric Invasive Group A Streptococcus (iGAS) hospitalizations Colorado. We compared epidemiology, clinical features, and patient outcomes this outbreak to prior years.Between October 2022 April 2023, prospectively identified reviewed iGAS cases hospitalized patients at Children's Hospital Using laboratory specimen records, also retrospectively number with sterile site GAS-positive cultures across three time periods: pre-COVID-19 (January 2015-March 2020), height COVID-19 pandemic (April 2020-September 2022), (October 2022-April 2023).Among 96 cases, median age was 5.7 years old; 66% were male, 70% previously healthy, 39% required critical care, four died. Almost 60% had associated respiratory viral symptoms, 10% toxic shock syndrome, 4% necrotizing fasciitis. Leukopenia, bandemia, higher C-reactive protein values findings need for care. There significantly more during (9.9/month vs 3.9/month pre-pandemic 1.3/month pandemic), including pneumonia (28% 15% 0% pandemic) multifocal disease (17% 3% P < .001 all.Outbreak case numbers almost triple baseline. The high percentage symptoms suggests link coinciding surges viruses time. GAS can be severe evolve rapidly; features may help earlier identification critically ill children.

Language: Английский

Citations

10
Restrained memory CD8+T cell responses favors viral persistence and elevated IgG responses in patients with severe Long COVID DOI Creative Commons
Lucie Rodriguez, Ziyang Tan, Tadepally Lakshmikanth

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 13, 2024

Abstract During the COVID-19 pandemic it was widely described that certain individuals infected by SARS-CoV-2 experience persistent disease signs and symptoms, Long COVID, which in some cases is very severe with life changing consequences. To maximize our chances of identifying underpinnings this illness, we have focused on 121 most from >1000 patients screened specialized clinics Sweden Belgium. We restricted study to subjects objective measures organ damage or dysfunction, >3 months following a verified, but mild-to-moderate infection. By performing systems-level immunological testing comparisons controls fully convalescent similar mild/moderate episode, identify elevated serological responses COVID suggestive chronic antigen stimulation. Persistent viral reservoirs been proposed using multiple orthogonal methods for detection RNA protein plasma subset detectable antigens, minimal overlap across assays, no correlation symptoms immune measurements. Elevated serologic other hand were inversely correlated clonally expanded memory CD8 + T cells, indicating restrained clonal expansion enables persistence, exposure IgG responses, even if antigen-detection blood not universally possible.

Language: Английский

Citations

3
Towards understanding post-COVID-19 condition: A systematic meta-analysis of transcriptomic alterations with sex-specific insights DOI Creative Commons
Elena Cristina Rusu, Pablo Monfort-Lanzas, Laia Bertran

et al.

Computers in Biology and Medicine, Journal Year: 2024, Volume and Issue: 175, P. 108507 - 108507

Published: April 22, 2024

Post COVID-19 Condition (PCC), characterized by lingering symptoms post-acute COVID-19, poses clinical challenges, highlighting the need to understand its underlying molecular mechanisms. This meta-analysis aims shed light on transcriptomic landscapes and sex-specific dynamics intrinsic PCC.

Language: Английский

Citations

3
Long COVID as a Disease of Accelerated Biological Aging: An Opportunity to Translate Geroscience Interventions DOI
Areez Shafqat, Mary Clare Masters, Utkarsh Tripathi

et al.

Ageing Research Reviews, Journal Year: 2024, Volume and Issue: 99, P. 102400 - 102400

Published: June 28, 2024

Language: Английский

Citations

3
Inflammatory and Prothrombotic Biomarkers Contribute to the Persistence of Sequelae in Recovered COVID-19 Patients DOI Open Access
Nallely García‐Larragoiti,

Alan Cano‐Méndez,

Yeny Jimenez-Vega

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(24), P. 17468 - 17468

Published: Dec. 14, 2023

The presence of long COVID (LC) following SARS-CoV-2 infection is a common condition that affects the quality life patients and represents diagnostic challenge due to diversity symptoms may coexist. We still do not have accurate information regarding pathophysiological pathways generate LC, so it important know inflammatory immunothrombotic biomarker profiles their implications in order characterize risk subgroups establish early therapeutic strategies. performed determination biomarkers volunteers with previous diagnoses SARS-CoV-2. were analyzed plasma by flow cytometry, we von Willebrand factor (vWF) samples using ELISA. clinical variables or absence then analyzed. IL-6, sCD40L, p-Selectin, PSGL-1, PAI-1, tPA, D-Dimer, TF, Factor IX levels elevated groups especially subgroup metabolic syndrome (MetS). VWF found be increased sequelae MetS. Our results confirmed persistence an active state, identify population at provide adequate follow-up.

Language: Английский

Citations

8
Intrinsic factors behind long COVID: III. Persistence of SARS‐CoV‐2 and its components DOI
Nawal Abd El‐Baky, Amro Abd Al Fattah Amara, Vladimir N. Uversky

et al.

Journal of Cellular Biochemistry, Journal Year: 2023, Volume and Issue: 125(1)

Published: Dec. 14, 2023

Abstract Considerable research has been done in investigating SARS‐CoV‐2 infection, its characteristics, and host immune response. However, debate is still ongoing over the emergence of post‐acute sequelae infection (PASC). A multitude long‐lasting symptoms have reported several weeks after primary acute that resemble other viral infections. Thousands articles described various post‐COVID‐19 conditions. Yet, evidence around these health problems, reasons behind them, their molecular underpinnings are scarce. These persistent also known as long COVID‐19. The persistence and/or components tissues can lead to COVID. For example, presence nucleocapsid protein RNA was detected skin, appendix, breast some COVID patients. multiple anatomic sites, including non‐respiratory such adrenal gland, ocular tissue, small intestine, lymph nodes, myocardium, sciatic nerve. Distinctive spike sequence variants were found tissues. Interestingly, prolonged detection subgenomic observed across all tissues, sometimes same patient, which likely reflects recent but defective replication. Moreover, noticed throughout brain at autopsy, late 230 days following symptom onset among unvaccinated patients who died severe infection. Here, we review an intrinsic factor We highlight immunological consequences this persistence.

Language: Английский

Citations

7
Cardiovascular symptoms of PASC are associated with trace-level cytokines that affect the function of human pluripotent stem cell derived cardiomyocytes DOI Creative Commons
Jane E. Sinclair,

Courtney Vedelago,

Feargal J. Ryan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 12, 2024

ABSTRACT Globally, over 65 million individuals are estimated to suffer from post-acute sequelae of COVID-19 (PASC). A large number living with PASC experience cardiovascular symptoms (i.e. chest pain and heart palpitations) (PASC-CVS). The role chronic inflammation in these symptoms, particular persisting for >1 year after SARS-CoV-2 infection, remains be clearly defined. In this cross-sectional study, blood samples were obtained three different sites Australia i) a resolved infection (and no persistent i.e. ‘Recovered’), ii) prolonged PASC-CVS iii) negative individuals. Individuals PASC-CVS, relative Recovered individuals, had transcriptomic signature associated inflammation. This was accompanied by elevated levels pro-inflammatory cytokines (IL-12, IL-1β, MCP-1 IL-6) at approximately 18 months post-infection. These present trace amounts, such that they could only detected the use novel nanotechnology. Importantly, trace-level direct effect on functionality pluripotent stem cell derived cardiomyocytes vitro . not observed presence dexamethasone. Plasma proteomics demonstrated further differences between patients post-infection including enrichment complement coagulation proteins those symptoms. Together, data provide new insight into nanotechnology as possible diagnostic approach condition.

Language: Английский

Citations

2
Long COVID is associated with lower percentages of mature, cytotoxic NK cell phenotypes DOI Creative Commons
Tasha Tsao, Amanda M. Buck,

Lucile Grimbert

et al.

Journal of Clinical Investigation, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Language: Английский

Citations

2