Neuronal identity control at the resolution of a single transcription factor isoform DOI Creative Commons
Natalie Smolin, Mark Dombrovski, Bryce W. Hina

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 14, 2024

The brain exhibits remarkable neuronal diversity which is critical for its functional integrity. From the sheer number of cell types emerging from extensive transcriptional, morphological, and connectome datasets, question arises how capable generating so many unique identities. 'Terminal selectors' are transcription factors hypothesized to determine final identity characteristics in post-mitotic cells. Which function as terminal selectors level control they exert over different not well defined. Here, we establish a novel role factor

Язык: Английский

Connectome-constrained networks predict neural activity across the fly visual system DOI Creative Commons
Janne K. Lappalainen, Fabian Tschopp, Sridhama Prakhya

и другие.

Nature, Год журнала: 2024, Номер 634(8036), С. 1132 - 1140

Опубликована: Сен. 11, 2024

Язык: Английский

Процитировано

23
Neuronal parts list and wiring diagram for a visual system DOI Creative Commons
Arie Matsliah, Szi-chieh Yu, Krzysztof Kruk

и другие.

Nature, Год журнала: 2024, Номер 634(8032), С. 166 - 180

Опубликована: Окт. 2, 2024

Язык: Английский

Процитировано

23
A split-GAL4 driver line resource forDrosophilaneuron types DOI Creative Commons
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 10, 2024

Abstract Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Язык: Английский

Процитировано

12
Mapping model units to visual neurons reveals population code for social behaviour DOI Creative Commons
Benjamin R. Cowley, Adam J. Calhoun,

Nivedita Rangarajan

и другие.

Nature, Год журнала: 2024, Номер 629(8014), С. 1100 - 1108

Опубликована: Май 22, 2024

Abstract The rich variety of behaviours observed in animals arises through the interplay between sensory processing and motor control. To understand these sensorimotor transformations, it is useful to build models that predict not only neural responses input 1–5 but also how each neuron causally contributes behaviour 6,7 . Here we demonstrate a novel modelling approach identify one-to-one mapping internal units deep network real neurons by predicting behavioural changes arise from systematic perturbations more than dozen neuronal cell types. A key ingredient introduce ‘knockout training’, which involves perturbing during training match experiments. We apply this model transformations Drosophila melanogaster males complex, visually guided social 8–11 visual projection at interface optic lobe central brain form set discrete channels 12 , prior work indicates channel encodes specific feature drive particular 13,14 Our reaches different conclusion: combinations neurons, including those involved non-social behaviours, male interactions with female, forming population code for behaviour. Overall, our framework consolidates effects elicited various into single, unified model, providing map stimulus type behaviour, enabling future incorporation wiring diagrams 15 model.

Язык: Английский

Процитировано

12
Comparative connectomics of the descending and ascending neurons of theDrosophilanervous system: stereotypy and sexual dimorphism DOI Creative Commons
Tomke Stürner, P. D. Brooks, Laia Serratosa Capdevila

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 6, 2024

In most complex nervous systems there is a clear anatomical separation between the nerve cord, which contains of final motor outputs necessary for behaviour, and brain. insects, neck connective both physical information bottleneck connecting brain ventral cord (VNC, spinal analogue) comprises diverse populations descending (DN), ascending (AN) sensory neurons, are crucial sensorimotor signalling control. Integrating three separate EM datasets, we now provide complete connectomic description neurons female system

Язык: Английский

Процитировано

11
Temporal and Notch identity determine layer targeting and synapse location of medulla neurons DOI Creative Commons
Isabel Holguera, Yee‐Chun Chen, Yu‐Chieh Chen

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 6, 2025

How specification mechanisms that generate neural diversity translate into specific neuronal targeting, connectivity, and function in the adult brain is not understood. In medulla region of Drosophila optic lobe, progenitors different neurons a fixed order by sequentially expressing series temporal transcription factors as they age. Then, Notch signaling intermediate further diversifies progeny. By establishing birth neurons, we found their identity correlates with depth neuropil targeting brain, for both local interneurons projection neurons. We show this identity-dependent unfolds early development genetically determined. leveraging Electron Microscopy reconstruction fly determined synapse location lobe neuropils find it significantly associated status. Moreover, all putative same predicted share similar location, indicating ensembles layers encode visual functions. conclusion, status can predict function, linking developmental patterning connectivity functional features brain.

Язык: Английский

Процитировано

1
Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation DOI Open Access
Tanya Wolff, Mark Eddison, Nan Chen

и другие.

Опубликована: Янв. 21, 2025

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent situ hybridization. About neuropeptides we examined were expressed only few cells, while rest dozens hundreds cells. Neuropeptide receptors more broadly at lower levels. Using our GAL4 drivers mark found 51 85 types least one 21 multiple neuropeptides. Surprisingly, all co-expressed molecule neurotransmitter. Finally, used identify whose activation affects sleep, identified other link circadian clock CX. well-characterized genetic information on neurotransmitter provide should enhance studies

Язык: Английский

Процитировано

1
Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation DOI Open Access
Tanya Wolff, Mark Eddison, Nan Chen

и другие.

Опубликована: Фев. 28, 2025

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent situ hybridization. About neuropeptides we examined were expressed only few cells, while rest dozens hundreds cells. Neuropeptide receptors more broadly at lower levels. Using our GAL4 drivers mark found 51 85 types least one 21 multiple neuropeptides. Surprisingly, all co-expressed neurotransmitter. Finally, used identify whose activation affects sleep, identified other link circadian clock CX. well-characterized genetic information on neurotransmitter provide should enhance studies

Язык: Английский

Процитировано

1
Connectomic reconstruction predicts visual features used for navigation DOI Creative Commons
Dustin Garner, Emil Kind,

Jennifer Yuet Ha Lai

и другие.

Nature, Год журнала: 2024, Номер 634(8032), С. 181 - 190

Опубликована: Окт. 2, 2024

Язык: Английский

Процитировано

7
Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation DOI Creative Commons
Tanya Wolff, Mark Eddison, Nan Chen

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 22, 2024

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent

Язык: Английский

Процитировано

6