Germination Behavior and Geographical Information System-Based Phenotyping of Root Hairs to Evaluate the Effects of Different Sources of Black Soldier Fly (Hermetia illucens) Larval Frass on Herbaceous Crops

Plants, Год журнала: 2024, Номер 13(2), С. 230 - 230

Опубликована: Янв. 14, 2024

Insect larval frass has been proposed as a fertilizer and amendment, but methods for testing its effects on plants are poorly developed need standardization. We obtained different types of black soldier fly (Hermetia illucens) via the factorial combination (a) two insect diets, follows: G (Gainesville = 50% wheat bran, 30% alfalfa meal, 20% maize meal) W (43% sheep whey + 57% seeds); (b) thermal treatments: NT untreated T treated at 70 °C 1 h. tested germination cress (Lepidium sativum L.) (Triticum durum Desf.) by applying 1:2 w:w water extracts 0, 25, 50, 75 100% concentration. Standardizing content before extraction affected chemical composition. Frass showed high electrical conductivity (8.88 to 13.78 mS cm−1). The diet was suppressive towards Escherichia coli lower nitrates (e.g., WNT 40% than GNT) concentration-dependent phytotoxic effect germinating plants. At 25% concentration, indices were 4.5 40-fold those 100%. Root shoot length root hair area concentration in were, respectively, 4.53 2 times higher 100%), whereas treatment few or inconclusive. On barley (Hordeum vulgare grown micropots silty loam soil, mass reduced 37% extract A quick procedure surface based geographic information system (GIS) may provide fast method incorporating phenotyping evaluation. results indicate that below-ground structures be addressed research effects. For this, phyotoxicity tests should encompass dilutions, standardized direction canonical procedures allow comparisons.

Язык: Английский

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A comprehensive characterization of Hermetia illucens derived chitosan produced through homogeneous deacetylation

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 271, С. 132669 - 132669

Опубликована: Май 25, 2024

The increasing demand for chitin and chitosan is driving research to explore alternative sources crustaceans. Insects, particularly bioconverters as Hermetia illucens, are promising substitutes they process food industry waste into valuable molecules, including chitin. Chitosan can be produced by deacetylation: hot deacetylation obtain a heterogeneous chitosan, the commonly produced, cold homogeneous not widely available. two different treatments lead arrangement of amine acetyl groups in structure, affecting its molecular weight, degree, biological activity. This first report on production chemical-physical characterization homogenous derived from H. illucens larvae, pupal exuviae, adults. work, addition our group, completes overview chitosan. yield values obtained exuviae (3 7 %) range insect (2-8 crustaceans (4-15 evaluation antioxidant activity antimicrobial properties against Gram-negative (Escherichia coli) Gram-positive (Micrococcus flavus) bacteria confirmed great versatility biomedical industrial applications suitability an source

Язык: Английский

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The Variety of Applications of Hermetia illucens in Industrial and Agricultural Areas—Review

Biology, Год журнала: 2022, Номер 12(1), С. 25 - 25

Опубликована: Дек. 22, 2022

Hermetia illucens (Diptera: Stratiomyidae, Linnaeus, 1978), commonly known as the black soldier fly (BSF), is a saprophytic insect, which in recent years has attracted significant attention from both scientific community and industry. The unrestrained appetite of larvae, ability to forage on various organic waste, rapid growth low environmental impact its breeding made it one insect species bred an industrial scale, hope producing fodder or other ingredients for animals. variety research related this shown that feed production not only benefit use. H. many features properties could be interest point view industries. Biomass utilization, chitin chitosan source, biogas, biodiesel production, entomoremediation, antimicrobial peptides, fertilizer potential wastes, are just some uses. This review brings together work four study into illucens. It summarizes current state knowledge introduces characteristics may helpful managing breeding, well use agro-industrial fields. Knowledge gaps under-studied areas were also highlighted, help identify future directions.

Язык: Английский

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Usage of chitosan from Hermetia illucens as a preservative for fresh Prunus species fruits: a preliminary analysis

Chemical and Biological Technologies in Agriculture, Год журнала: 2023, Номер 10(1)

Опубликована: Сен. 26, 2023

Abstract Background Fruit and vegetables are highly perishable. In an era where reducing food waste is absolutely essential, packaging important for maintaining the postharvest quality of these fresh products. Research working to reduce use synthetic materials, not safe environment human health. this perspective, chitosan emerges as a viable solution purpose, it biodegradable, biocompatible also application. The growing interest in using insects source chitin has allowed increased exploitation insect-based products recover valuable such biopolymers. black soldier fly ( Hermetia illucens L.) most widely reared species Europe feed production management. Results work, mature apricots Prunus armeniaca L.), nectarines persica vulgaris Mill.) yellow peaches var. laevis Gray) were coated with 0.5% 1% from pupal exuviae , applied by spraying stored at room temperature or 4 °C until they decay. Then, validate effectiveness polymer fruit preservation, several parameters including pH, TSS weight loss evaluated. Conclusions results showed that derived effective better than commercially available crustacean more stable some storage apricots, peaches. Thus, insects, especially confirmed alternative polymer. Graphical

Язык: Английский

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Inoculation with black soldier fly larvae alters the microbiome and volatile organic compound profile of decomposing food waste

Scientific Reports, Год журнала: 2023, Номер 13(1)

Опубликована: Март 15, 2023

Abstract The black soldier fly (BSF; Hermetia illucens ) is used in sustainable processing of many types organic waste. However, waste being decomposed by BSF produces strong odors, hindering more widespread application. odor components and how they are produced have yet to be characterized. We found that digestion food significantly alters the microbial flora, based on metagenomic analyses, generated, as shown thermal desorption gas chromatography mass spectrometry analysis. Inoculation with decreased production volatile sulfur compounds (dimethyl disulfide dimethyl trisulfide), which known released during methionine cysteine metabolism Lactobacillus Enterococcus bacteria. inoculation changed abundance diversity overall. These findings may help optimizing use for deodorization composting

Язык: Английский

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Hemp Waste as a Substrate for Hermetia illucens (L.) (Diptera: Stratiomyidae) and Tenebrio molitor L. (Coleoptera: Tenebrionidae) Rearing

Insects, Год журнала: 2023, Номер 14(2), С. 183 - 183

Опубликована: Фев. 13, 2023

The proper treatment of cannabis agricultural wastes can reduce the environmental impact its cultivation and generate valuable products. This study aimed to test potential as a substrate for rearing black soldier fly larvae (BSFL) yellow mealworms (MW). In case BSFL, replacing fibre component (straw) in with hemp waste increase nutritional value led bigger larvae. had lower P Mg, higher Fe Ca. Crude protein also varied based on size and/or content initial substrate, which was boosted by straw material. No other cannabinoids than cannabidiolic acid (CBDA), cannabigerolic (CBGA), cannabidiol (CBD) were found significant amounts MW, grew less material comparison wheat bran. Replacing bran smaller Ca, Fe, K, crude content, but Mg values. detected MW fed

Язык: Английский

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Bio-upcycling of cheese whey: Transforming waste into raw materials for biofuels and animal feed

Heliyon, Год журнала: 2024, Номер 10(12), С. e32700 - e32700

Опубликована: Июнь 1, 2024

Cheese whey (CW), by-product of cheese production, has potential as a valuable resource due to its nutritional composition. Although options for CW degradation have been explored, biological treatment with black soldier fly larvae (BSFL) not reported. This study evaluated the growth and composition BSFL in four experimental diets under different conditions. Results show that use allows larval development weight gain, also, conversion into biomass was up 0.215. Diets ED3 (fresh CW, 38 °C) ED4 room temperature) allowed higher accumulation (final 0.285 g); highest fat (12 % than control) observed (up 45.57 %), which had less protein. Moreover, amounts saturated fatty acids are generated. highlights importance an appropriate pretreatment designed specific waste control desired by-products.

Язык: Английский

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Biological Activity of Peptide Fraction Derived from Hermetia illucens L. (Diptera: Stratiomyidae) Larvae Haemolymph on Gastric Cancer Cells

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 1885 - 1885

Опубликована: Фев. 22, 2025

Gastric cancer (GC) is one of the leading causes cancer-related mortality worldwide, characterised by poor prognosis and limited responsiveness to chemotherapy. There a need for new more effective anticancer agents. Antimicrobial peptides (AMPs) represent promising class biomolecules this purpose. Naturally occurring in innate immune system, these can also exert cytotoxic effects against cells, earning them designation "anticancer peptides" (ACPs). They have potential be viable support current chemotherapy schedules due their selectivity cells minor propensity induce chemoresistance cells. Insects are an excellent source AMPs. Among them, its ability thrive hostile microorganism-rich environments, we isolated peptide fraction from Hermetia illucens L. (Diptera: Stratiomyidae) haemolymph evaluate possible activity. We tested Peptide Fractions (PFs) AGS KATO III gastric cell lines. Data obtained indicated that PFs, especially those resulting Escherichia coli Micrococcus flavus infection (to boost response), were able inhibit tumour growth inducing apoptosis or cycle arrest line-specific manner. These results further investigation into use antimicrobial produced insects as

Язык: Английский

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A metanalysis to evaluate the effects of substrate sources on the nutritional performance of black soldier fly larvae: implications for sustainable poultry feed

Poultry Science, Год журнала: 2023, Номер 103(2), С. 103299 - 103299

Опубликована: Ноя. 18, 2023

This meta-analysis presents an evaluation of substrate sources and their impact on the growth performance black soldier fly (BSF) larva. The database, compiled from Google Scholar, PubMed, Science Direct, focuses data concerning sources, environmental conditions, parameters BSF. Seven types substrates were analyzed, including Feed Waste, Manure, Fruits, Mix, Animal Source, Fermentation Residue, Food Waste. Waste group demonstrated highest DM content, while CP content was found in Source group. Higher larva meal Residues diets, respectively. survival rates observed BSF larvae fed Residues, Manure compared to Vegetable substrates. Fresh larval weight lower when used as a feed than pre-pupal Wet Weight surpassing those Substrate exhibited positive relationship with fresh weight, prepupal wet dry weight; length, mortality until prepupal, protein conversion, conversion ratio, food consumption, reduction rate bioconversion waste index, efficiency digested In conclusion, our findings underline that source composition are correlated nutritional meal.

Язык: Английский

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Effect of larval handling on black soldier fly life history traits and bioconversion efficiency

Frontiers in Veterinary Science, Год журнала: 2024, Номер 11

Опубликована: Янв. 15, 2024

Introduction The black soldier fly is considered the most promising insect species for mass production; however, information on effects of handling, which unavoidable during experimental trials and rearing practices, still limited. Materials methods To address this gap, three different manipulation intensities were tested 100 6-day-old larvae per replica (6 replicates/treatments) fed Gainesville diet: (1) hard-handled (HH), underwent continuous until end larval stage, (2) soft-handled (SH), manipulated after appearance first prepupa, (3) no-handled (NH), remained untouched. Every 4 days from beginning to manipulations lasted 30 min occurred under laboratory conditions (20°C). During sampling operations, at least randomly extracted, washed, dried, weight-mimicked. At all boxes untouched adult emergency rate sex ratio evaluated dead flies. Data statistically analyzed using IBM SPSS V20.0.0 software significance level was p < 0.05. Results stage 8.2 both HH SH ( > 0.05). Despite being manipulated, no difference also observed in final weight (HH, 160 mg; SH, 150 0.05) survival 96.2%; 94.5%; did not influence bioconversion capacity (bioconversion efficiency corrected residue: HH, 14.3%; 12.91%; reduction rate: 58.4%; 55.9%; waste index: 7.28%/day; 7.25%/day; Finally, development time larva (about 20.7; 0.05), (NH: 92.8%; SH: 89.5%; HH: 82.7%) (~1.2% male flies) affected by handling Discussion In conclusion, procedures used current study life history traits fly. However, further studies are needed evaluate if protocols various scales, colony strain or other may suggest a scenario confirm results.

Язык: Английский

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