ALDH2 Deficiency Aggravates Lung Fibrosis through Mitochondrial Dysfunction and Aging in Fibroblasts

American Journal Of Pathology, Год журнала: 2024, Номер 194(8), С. 1458 - 1477

Опубликована: Май 21, 2024

Язык: Английский

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Molecular Mechanisms of Oxidative Stress Relief by CAPE in ARPE−19 Cells

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(4), С. 3565 - 3565

Опубликована: Фев. 10, 2023

Caffeic acid phenylethyl ester (CAPE) is an antioxidative agent originally derived from propolis. Oxidative stress a significant pathogenic factor in most retinal diseases. Our previous study revealed that CAPE suppresses mitochondrial ROS production ARPE-19 cells by regulating UCP2. The present explores the ability of to provide longer-term protection RPE and underlying signal pathways involved. were given pretreatment followed t-BHP stimulation. We used situ live cell staining with CellROX MitoSOX measure accumulation; Annexin V-FITC/PI assay evaluate apoptosis; ZO-1 immunostaining observe tight junction integrity cells; RNA-seq analyze changes gene expression; q-PCR validate data; Western Blot examine MAPK pathway activation. significantly reduced both cellular mitochondria overproduction, restored loss expression, inhibited apoptosis induced also demonstrated reverses overexpression immediate early genes (IEGs) activation p38-MAPK/CREB pathway. Either genetic or chemical deletion UCP2 largely abolished protective effects CAPE. restrained generation preserved structure against oxidative stress-induced apoptosis. These mediated via regulation p38/MAPK-CREB-IEGs

Язык: Английский

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Uncoupling protein 2 deficiency of non-cancerous tissues inhibits the progression of pancreatic cancer in mice

Hepatobiliary & pancreatic diseases international, Год журнала: 2022, Номер 22(2), С. 190 - 199

Опубликована: Дек. 14, 2022

Язык: Английский

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AKAP8 regulates R-loop balance and promotes growth of lung carcinoma cell

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 16, 2024

Background RNA:DNA hybrid structure known as R-loop, which forms during transcription plays a pivotal roles in transcriptional regulation. Dysregulation of R-loop dynamics disrupt normal DNA replication or RNA transcription, potentially leading to disturbances cell metabolism, abnormal proliferation and disease progression.Methods Interactome data nucleic AKAPs were collected analyzed nominate the candidate AKAP8 (A-kinase-anchoring protein 8) binding protein. The interaction confirmed by co-immunoprecipitation immunofluorescence. resolution DDX5 identified interact with its abundance was estimated. knock down lines constructed. mRNA profile differential expressed genes analyzed. Downstream target gene UCP2 upregulate level promoter Cell growth migration lung carcinoma line depletion not also investigated EdU, colony formation wound healing essay. Expression score comparing cancer tissue tissue, correlation between survival possibility patients expression AKAP8, investigated.Results This study that interacted within cells. Depletion resulted perturbation genomic balance transcription. Evidences shown regulated chromatin associated level. Furthermore, found enhance uncoupling well alleviate promoter, promoted cell. lower high expression.Conclusions elucidates novel modulating cooperation is one motivators for contributed mitochondrial metabolism. insight may offer prognostic significance adenocarcinoma exhibiting higher expression.

Язык: Английский

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Inhibition of Distal Glycolytic Enzymes Suppresses Fibroblast Activation without Deleterious Bioenergetic Effects

American Journal of Respiratory Cell and Molecular Biology, Год журнала: 2024, Номер 70(6), С. 519 - 521

Опубликована: Май 31, 2024

Язык: Английский

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