Approaches to Improve Endometrial Receptivity in Case of Repeated Implantation Failures

Frontiers in Cell and Developmental Biology, Год журнала: 2021, Номер 9

Опубликована: Март 16, 2021

Repeated implantation failures are a constant challenge in reproductive medicine with significant impact both on health providers and infertile couples. Several approaches have been proposed so far as effective; however, accumulative data clarified that most of the treatment options do not evidence base for generalized application to be suggested by relevant societies. Implantation attributed either poor quality embryos or defected endometrial receptivity. The current review aims summarize systematic way all new trends managing RIF via interference authors focus mainly, but exclusively, injury prior embryo transfer priming autologous cells biological agents. To this direction, search Pubmed database has conducted taking into account emerged last two decades. All interventions herein presented analyzed terms outcomes. It is evident properly powered designed randomized trials needed support standard approach will safely incorporated national international guidelines.

Язык: Английский

---

Histological diagnostic criterion for chronic endometritis based on the clinical outcome

BMC Women s Health, Год журнала: 2021, Номер 21(1)

Опубликована: Март 4, 2021

Abstract Background The diagnostic criteria of chronic endometritis remain controversial in the treatment for infertile patients. Methods A prospective observational study was conducted a single university from June 2014 to September 2017. Patients who underwent frozen-thawed blastocyst transfer with hormone replacement cycle after histological examination presence were enrolled. Four used define according number plasma cells same group patients: 1 or more (≥ 1) cells, 2 2), 3 3), 5 5) 10 high-power fields. Pregnancy rates, live birth and miscarriage rates non-chronic groups defined each criterion calculated. logistic regression analysis performed births using eight explanatory variables (seven infertility factors endometritis). receiver operating characteristic curve drawn optimal cut-off value Results total 69 patients registered 53 finally analyzed exclusion. When designated as ≥ cell endometrial stroma per fields, pregnancy rate, rate 63.0% vs. 30.8%, 51.9% 7.7%, 17.7% 75% groups, respectively. This resulted highest among smallest P values between groups. In analysis, an variable negatively affecting objective only when diagnosed obtained one found fields (sensitivity 87.5%, specificity 64.9%). Conclusions Chronic should be According this criterion, adversely affected rate.

Язык: Английский

---

Analysis of pregnancy outcomes in patients with recurrent implantation failure complicated with chronic endometritis

Frontiers in Cell and Developmental Biology, Год журнала: 2023, Номер 11

Опубликована: Фев. 13, 2023

Chronic endometritis (CE) has been considered to reduce uterine receptivity and negatively affect reproductive outcomes for in vitro fertilization-embryo transfer (IVF-ET) patients, especially people undergoing recurrent implantation failure (RIF). To investigate the effects of antibiotic platelet-rich plasma (PRP) therapy on pregnancy following frozen-thawed embryo (FET) RIF patients with CE, endometrial specimens 327 obtained by scratching during mid-luteal phase were immunostained multiple myeloma oncogene-1 (Mum-1)/syndecan-1 (CD138). CE given antibiotics PRP treatment. According Mum-1+/CD138+ plasmacytes after treatment, divided into persistent weak positive (+) group, (-) group non-CE group. FET was performed basic characteristics three groups compared. Among 117 complicated prevalence 35.78%. The strong 27.22% that 8.56%. 70.94% converted negative There no significant difference characteristics, including age, BMI, AMH, AFC, infertility years, types, number previous transplant cycles, thickness transplantation day transplanted embryos (p > 0.05); rate hCG, clinical significantly increased compared < .01), live birth also improved .05). Whereas, early abortion 12.70%, which higher than After multivariate analysis, failed cycles remained independent factors associated rate, while only be factor rate. It is recommended perform CE-related examination RIF. Antibiotic treatment can improve conversion cycle.

Язык: Английский

---

Identification of Uterine Microbiota in Infertile Women Receiving in vitro Fertilization With and Without Chronic Endometritis

Frontiers in Cell and Developmental Biology, Год журнала: 2021, Номер 9

Опубликована: Авг. 13, 2021

In vitro fertilization (IVF) is an important assisted reproductive technology in treating infertility, whose failure rate still high. Studies suggested that uterine microbiota are related to women’s diseases and persisting intrauterine bacterial infectious conditions, such as chronic endometritis (CE), impairing the pregnant processes. However, relationship between IVF outcomes open question. present study, 94 patients diagnosed with infertility were enrolled divided into CE (E group, n = 25) non-CE (NE 69) groups depending on hysteroscopy immunohistochemistry. Subsequently, E (Ep, 8 Enp, 17) NE (NEp, 41 NEnp, 28) pregnancy non-pregnancy outcomes, respectively. The fluids collected microbial profiles examined through V4 region of 16S rRNA gene high-throughput sequencing. results demonstrated had significantly lower clinical compared (32 vs. 58.42%, p 0.0014). relative abundances Proteobacteria Acidobacteria higher whereas high Actinobacteria Fusobacteria observed group at phylum level. At genus level, Gardnerella groups, which referred negative outcome. conclusion, may be a key factor for outcome after IVF, plays pivotal role, diversity serve biomarker forecast success

Язык: Английский

---

Epithelial Pyroptosis in Host Defense

Journal of Molecular Biology, Год журнала: 2021, Номер 434(4), С. 167278 - 167278

Опубликована: Окт. 8, 2021

Язык: Английский

---

The human periconceptional maternal-embryonic space in health and disease

Physiological Reviews, Год журнала: 2023, Номер 103(3), С. 1965 - 2038

Опубликована: Фев. 16, 2023

Pregnancy is established during the periconceptional period as a continuum beginning with blastocyst attachment to endometrial epithelial surface followed by embryo invasion and placenta formation. This sets foundation for child mother's health pregnancy. Emerging evidence indicates that prevention of downstream pathologies in both embryo/newborn pregnant mother may be possible at this stage. In review, we discuss current advances space, including preimplantation human maternal endometrium. We also role decidua, maternal-embryonic interface, dialogue between these elements, importance microbiome implantation process Finally, myometrium space review its determining pregnancy health.

Язык: Английский

---

Endometrial preparation protocols did not impact outcomes after frozen-thaw embryo transfer for chronic endometritis treatment

Contraception and Reproductive Medicine, Год журнала: 2025, Номер 10(1)

Опубликована: Апрель 7, 2025

Intended to investigate the potential effects of endometrial preparation procedures on results subsequent frozen embryo transfer (FET) cycles in infertile women with chronic endometritis (CE) following a single course antibiotic therapy. From July 2020 December 2023, 773 patients participated retrospective research. CD138 was immunostained tissues taken during proliferative phase. CE suggested by presence CD138-positive cells stromal cells. Oral antibiotics were administered every patient diagnosis. After one drug treatment, had and transfers. This study compared impact different strategies pregnancy outcomes. Four regimens, hormone replacement treatment (HRT), ovulation induction, natural cycle, gonadotrophin-releasing agonist-HRT (GnRH agonist-HRT), did not significantly alter outcome CE. Compared other groups, induction cycle group higher clinical rate implantation rate. In addition, miscarriage is lower populations. Patients treated who received HRT, GnRH agonist-HRT, differ substantially (P > 0.05). see any change outcomes as result procedures.

Язык: Английский

---

AI-algorithm training and validation for identification of endometrial CD138+ cells in infertility-associated conditions; polycystic ovary syndrome (PCOS) and recurrent implantation failure (RIF)

Journal of Pathology Informatics, Год журнала: 2024, Номер 15, С. 100380 - 100380

Опубликована: Апрель 29, 2024

Endometrial CD138+ plasma cells serve as a diagnostic biomarker for endometrial inflammation, and their elevated occurrence correlates positively with adverse pregnancy outcomes. Infertility-related conditions like polycystic ovary syndrome (PCOS) recurrent implantation failure (RIF) are closely associated systemic local chronic inflammatory status, wherein cell accumulation could also contribute to pathology. Current methods quantifying typically involve laborious time-consuming microscopic assessments of only few random areas from slide. These have limitations in accurately representing the entire slide susceptible significant biases arising intra- interobserver variations. Implementing artificial intelligence (AI) identification enhance accuracy, reproducibility, reliability analysis.

Язык: Английский

---

Occurrence of chronic endometritis in different types of human adenomyosis

Reproductive Medicine and Biology, Год журнала: 2021, Номер 21(1)

Опубликована: Окт. 15, 2021

Human adenomyosis has an adverse effect on female fertility. Exact mechanistic basis is still unclear. We investigated the occurrence of chronic endometritis (CE) in different types human adenomyosis.

Язык: Английский

---

Treatment following hysteroscopy and endometrial diagnostic biopsy increases the chance for live birth in women with chronic endometritis

American Journal of Reproductive Immunology, Год журнала: 2021, Номер 86(5)

Опубликована: Июль 4, 2021

Repeated implantation failure and recurrent pregnancy loss are associated with chronic endometritis, a persistent endometrial inflammation. Its diagnosis treatment may increase live birth rates. The aim of this study was to assess the effectiveness diagnostic biopsy subsequent antibiotic in cases endometritis on reproductive outcomes over long observation period.We conducted historical cohort (2014-2018) at our University-based infertility center that included women (n = 108) repeated or without known pathologies either condition. Forty-one underwent hysteroscopy only (reference group); remaining 67 underwent, addition hysteroscopy, an immunohistochemically staining for CD138 detect plasma cells (biopsy group). If one more were detected, treated doxycycline 100 mg twice day orally 2 weeks. We performed stratified survival analysis (Kaplan-Meier) Cox regression.The group had higher chances (hazard ratio 2.28; 95% confidence interval 1.23-4.24; p .009) 2.76; 1.30-5.87; .008) compared reference group. In sensitivity analysis, did not affect outcome.Endometrial followed by case birth.

Язык: Английский

---