Amniotic Fluid-Derived Stem Cells: An Overlooked Source of Stem Cells for Translational Research

DNA and Cell Biology, Год журнала: 2025, Номер 44(3), С. 144 - 152

Опубликована: Март 1, 2025

Amniotic fluid-derived stem cells (AFSCs) represent a promising yet underutilized resource for research and clinical applications. While AFSCs share phenotypic functional characteristics with derived from somatic tissues such as bone marrow, adipose tissue, placenta, umbilical cord, their unique developmental origin grants them several superior qualities. These include enhanced multipotency, tissue-specific genotypic profiles, the ability to form single-cell colonies. Such features position highly valuable translational tissue engineering. This review seeks underscore distinctive attributes of AFSCs, particularly relevance in By emphasizing these qualities, we aim stimulate further exploration into use patient-specific induced pluripotent organoid development, potentially unlocking full therapeutic potential. The capabilities make an exceptional candidate advancing regenerative medicine, offering new avenues treating variety conditions that currently have limited options.

Язык: Английский

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Neural Stem Cell-Derived Small Extracellular Vesicles: key Players in Ischemic Stroke Therapy – A Comprehensive Literature Review

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 4279 - 4295

Опубликована: Май 1, 2024

Ischemic stroke, being a prominent contributor to global disability and mortality, lacks an efficacious therapeutic approach in current clinical settings. Neural stem cells (NSCs) are type of cell that only found inside the nervous system. These can differentiate into various kinds cells, potentially regenerating or restoring neural networks within areas brain have been destroyed. This review begins by providing introduction existing approaches for ischemic followed examination promise limits associated with utilization NSCs treatment stroke. Subsequently, comprehensive overview was conducted synthesize literature on underlying processes cell-derived small extracellular vesicles (NSC-sEVs) transplantation therapy context mechanisms encompass neuroprotection, inflammatory response suppression, endogenous nerve vascular regeneration facilitation. Nevertheless, translation NSC-sEVs is hindered challenges such as inadequate targeting efficacy insufficient content loading. In light these limitations, we compiled advancements utilizing modified treating stroke based methods vesicle modification. conclusion, examining NSC-sEVs-based anticipated be both fundamental applied investigations about

Язык: Английский

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Huc-MSCs-derived exosomes alleviate non-alcoholic steatohepatitis by regulating macrophages polarization through miR-24-3p/STING axis

Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 21, 2025

There's a scarcity of drugs effective against nonalcoholic steatohepatitis (NASH). Exosomes from Human umbilical cord mesenchymal stem cells (huc-MSCs) show potential in managing glycolipid metabolism and the immune response. Therefore, further investigations are required to explore their application NASH underlying mechanisms. C57BL/6J mice were fed with western diet for 12 weeks induce NASH, huc-MSCs exosomes (MSCs-exo) administered during feeding period. The effect MSCs-exo was evaluated by monitoring changes body weight, fat distribution, blood glucose, insulin levels, analyzing pathological alterations liver tissue. Mechanism carried out using flow cytometry, immunofluorescence staining, other experimental techniques. could reduce fat, inflammation, fibrosis, improved alleviate progression NASH. Besides, decrease macrophage accumulation liver, encouraging M2 over M1 polarization. Furthermore, our study found that had high expression miR-24-3p, which may regulate polarization targeting interferon-stimulated genes (STING) gene macrophages, its overexpression amplifying MSCs-exo's benefits. These findings suggest therapeutic on be attributed regulation through miR-24-3p STING. This provides scientific basis future clinical application.

Язык: Английский

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Administration Strategy-Dependent Mechanisms and Effects of Human Adipose Tissue Stem Cell Extracellular Vesicles in Mouse Allergic Rhinitis Treatment

Cell Transplantation, Год журнала: 2025, Номер 34

Опубликована: Апрель 1, 2025

We previously found that intravenous injection of extracellular vesicles (EVs) from human adipose tissue–derived stem cells (hADSC) could ameliorate allergic rhinitis (AR) in mice through immunomodulatory effects. In clinical trials, nasal delivery has been an attractive treatment for AR. sought to determine whether there are differences the therapeutic effects between caudal and their combination. treated AR with ADSC-EVs via vein, cavity, or both. After treatment, were re-sensitized indices behavior, mucosa morphology, cytokine secretion under different modes administration calculated. The resultes show tail nasal, combined effectively relieve inflammatory infiltration mice, reduce IgE, IL-4, other factors, alleviate Th1/Th2 imbalance. Injection delivery, as well combination, alleviated symptoms mice. Nasal a better effect when response is mild. It be speculated have excellent properties AR, can selected stages therapy.

Язык: Английский

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Identifying TNFSF4low-MSCs superiorly treating idiopathic pulmonary fibrosis through Tregs differentiation modulation

Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)

Опубликована: Апрель 20, 2025

Язык: Английский

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Extracellular vesicles: Messengers of allergic immune responses and novel therapeutic strategy

European Journal of Immunology, Год журнала: 2024, Номер 54(5)

Опубликована: Фев. 15, 2024

Abstract Extracellular vesicles (EVs) are nanosized particles released by nearly every cell type across all kingdoms of life. As a result, EVs ubiquitously present in various human body fluids. Composed lipid bilayer, encapsulate proteins, nucleic acids, and metabolites, thus playing crucial role immunity, for example, enabling intercellular communication. More recently, there has been increasing evidence that can also act as key regulators allergic immune responses. Their ability to facilitate cell‐to‐cell contact transport variety different biomolecules enables active modulation both innate adaptive processes associated with reactions. A comprehensive understanding the intricate mechanisms underlying interactions among allergens, cells, is imperative develop innovative strategies controlling This review highlights recent roles host cell‐ bacteria‐derived diseases, presenting experimental clinical underscores their significance. Additionally, therapeutic potential allergy management outlined, along challenges targeted delivery cargo stability use. Optimization EV composition targeting holds promise advancing translational applications establishing biomarkers or safe therapeutics assessing For these reasons, represent promising avenue our processes.

Язык: Английский

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Investigating Experimental Treatments for Rhinitis: A State-of-the-Art Systematic Review

Ear Nose & Throat Journal, Год журнала: 2024, Номер unknown

Опубликована: Янв. 11, 2024

Background: Rhinitis is a common inflammatory condition that affects the nasal passages, significantly impacting quality of life and placing considerable burden on healthcare systems. While traditional treatments offer limited relief, there growing interest in novel therapies. This systematic review aims to analyze investigational new for rhinitis. Methods: A search was conducted ClinicalTrials.gov, World Health Organization International Clinical Trials Registry Platform, European Union Register, as well PubMed, Web Science, Cochrane Library. Both ongoing completed clinical trials exploring innovative therapies rhinitis, including immunotherapy, probiotics, stem cell therapy, were included. Results: compiled information from 74 trials—51 23 ongoing—focused significant portion studies (44) focused various forms which showed potential long-term effectiveness had high safety profile. Another seven investigated probiotics treatment method, yielding mixed results, though they did show promise managing symptoms, particularly when combined with other treatments. The are primarily investigating smaller number looking at therapy. shows continued exploration diverse Conclusion: study highlights emerging rhinitis improve patient outcomes enhance life. Continued research recommended developing more effective, personalized, targeted therapeutic strategies

Язык: Английский

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Extracellular vesicles from adipose stem cells ameliorate allergic rhinitis in mice by immunomodulatory

Frontiers in Immunology, Год журнала: 2023, Номер 14

Опубликована: Дек. 8, 2023

Background Human adipose tissue-derived stem cells (hADSCs) exert potent immunosuppressive effects in the allogeneic transplantation treatment. In mouse model of allergic rhinitis (AR), ADSCs partially ameliorated AR. However, no study has evaluated potential therapeutic hADSC-derived extracellular vesicles (hADSC-EVs) on Methods Female BALB/c mice were sensitized and challenged with ovalbumin (OVA) to induce One day after last nasal drop, each group received phosphate buffered saline (PBS) or hADSC-EVs Associated symptoms biological changes then assessed. Results hADSC-EV treatment significantly alleviated symptoms, reduced inflammatory infiltration. Serum levels OVA-specific IgE, interleukin (IL)-4 interferon (IFN)-γ all reduced. The mRNA IL-4 IFN-γ spleen also changed accordingly. T helper (Th)1/Th2 cell ratio increased. efficacy index was higher than that human-derived MSCs published reports MSC ADSC-EVs exhibited a greater most measures when compared our previous involving ADSCs. Conclusion These results demonstrated could ameliorate AR by modulating cytokine secretion Th1/Th2 balance. potentially be viable strategy for Further animal studies are needed elucidate underlying mechanisms optimize clinical protocols.

Язык: Английский

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The function and mechanism of Human nasal mucosa-derived mesenchymal stem cells in allergic rhinitis in mice

Inflammation Research, Год журнала: 2024, Номер 73(10), С. 1819 - 1832

Опубликована: Авг. 24, 2024

To investigate the immunomodulatory effects and potential mechanisms of human nasal mucosa-derived mesenchymal stem cells(hNMSCs) on mouse allergic rhinitis, to compare them with umbilical cord-derived cells (hUCMSCs).

Язык: Английский

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Application of Nanoparticles for Immunotherapy of Allergic Rhinitis

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 12015 - 12037

Опубликована: Ноя. 1, 2024

Allergen Immunotherapy (AIT) is the only etiological therapeutic method available for allergic rhinitis (AR). Currently, several options AIT in market, such as subcutaneous immunotherapy (SCIT) and sublingual (SLIT), have different routes of administration. These traditional methods achieved encouraging outcomes clinic. However, side effects associated with these raised need innovative approaches that improve safety, shorten course treatment increase local drug concentration. Nanoparticles (NPs) are particles ranging size from 1 to 100 nm, which been hired potential adjuvants AIT. NPs can be employed agents modulating immune responses AR or/and carriers loading proteins, peptides or DNA molecules. This review focuses on kinds nanoparticle delivery systems, including chitosan nanoparticles, exosomes, metal viral nanoparticles. We summarized advantages limitations rhinitis. Overall, expected a option AR, requires more in-depth studies long-term validation.

Язык: Английский

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