Pseudomonas aeruginosa adaptation and evolution in patients with cystic fibrosis

Nature Reviews Microbiology, Год журнала: 2020, Номер 19(5), С. 331 - 342

Опубликована: Ноя. 19, 2020

Язык: Английский

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Role of Efflux Pumps on Antimicrobial Resistance in Pseudomonas aeruginosa

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(24), С. 15779 - 15779

Опубликована: Дек. 13, 2022

Antimicrobial resistance is an old and silent pandemic. Resistant organisms emerge in parallel with new antibiotics, leading to a major global public health crisis over time. Antibiotic may be due different mechanisms against classes of drugs. These are usually found the same organism, giving rise multidrug-resistant (MDR) extensively drug-resistant (XDR) bacteria. One mechanism that closely associated emergence MDR XDR bacteria efflux drugs since pump can transport In Gram-negative bacteria, pumps present two configurations: transmembrane protein anchored inner membrane complex formed by three proteins. The tripartite has membrane, periplasmic protein, porin outer membrane.

Язык: Английский

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Biofilm Formation and Control of Foodborne Pathogenic Bacteria

Molecules, Год журнала: 2023, Номер 28(6), С. 2432 - 2432

Опубликована: Март 7, 2023

Biofilms are microbial aggregation membranes that formed when microorganisms attach to the surfaces of living or nonliving things. Importantly, biofilm properties provide with protection against environmental pressures and enhance their resistance antimicrobial agents, contributing persistence toxicity. Thus, bacterial formation is part survival mechanism. However, if foodborne pathogens form biofilms, risk disease infections can be greatly exacerbated, which cause major public health risks lead adverse economic consequences. Therefore, research on biofilms removal strategies very important in food industry. Food waste due spoilage within industry remains a global challenge sustainability security supplies. This review describes formation, elaborates problem associated industry, enumerates several kinds common summarizes current used eliminate control harmful introduces emerging strategies, emphasizes future development prospects respect biofilms.

Язык: Английский

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Evolution and host-specific adaptation ofPseudomonas aeruginosa

Science, Год журнала: 2024, Номер 385(6704)

Опубликована: Июль 4, 2024

The major human bacterial pathogen

Язык: Английский

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β-lactam Resistance in Pseudomonas aeruginosa: Current Status, Future Prospects

Pathogens, Год журнала: 2021, Номер 10(12), С. 1638 - 1638

Опубликована: Дек. 18, 2021

Pseudomonas aeruginosa is a major opportunistic pathogen, causing wide range of acute and chronic infections. β-lactam antibiotics including penicillins, carbapenems, monobactams, cephalosporins play key role in the treatment P. However, significant number isolates these bacteria are resistant to β-lactams, complicating infections leading worse outcomes for patients. In this review, we summarize studies demonstrating health economic impacts associated with β-lactam-resistant aeruginosa. We then describe how β-lactams bind inhibit penicillin-binding proteins that required synthesis remodelling peptidoglycan. Resistance multifactorial can involve changes target protein, protein 3, essential cell division; reduced uptake or increased efflux β-lactams; degradation by expression altered substrate specificity an AmpC β-lactamase, acquisition β-lactamases through horizontal gene transfer; biofilm formation metabolism. The current understanding mechanisms discussed. Lastly, important knowledge gaps identified, possible strategies enhancing effectiveness treating considered.

Язык: Английский

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Potential synergistic activity of quercetin with antibiotics against multidrug-resistant clinical strains of Pseudomonas aeruginosa

PLoS ONE, Год журнала: 2020, Номер 15(11), С. e0241304 - e0241304

Опубликована: Ноя. 6, 2020

Development of drug resistance in opportunistic pathogens is one the major healthcare challenges associated with infection management. Combination therapy has many advantages due to simultaneous action two drugs on separate cellular targets. However, selection should offer safety and synergistic interaction against most strains. Here, efficacy antibiotics combination quercetin, a natural flavonoid capable targeting quorum sensing was tested biofilm-forming Pseudomonas aeruginosa strains previously isolated from catheter urinary tract infection. Based antibiotic susceptibility pattern, effect quercetin selected (levofloxacin, ceftriaxone, gentamycin, tobramycin amikacin) at fractional concentrations MIC by checkerboard method inhibitory concentration index (FIC i ) calculated estimate effect. Effect combinations were further using time-kill assay, biofilm formation cell viability. Cytotoxicity assays performed Human Embryonic Kidney 293T cells (HEK-293T) effective respective controls. The viability drastically affected ≥80% inhibition. In vitro studies showed that all could exert significant killing (68 85%) decreased rate significantly reducing P . (p<0.05). attributed its properties. These findings indicate along existing can potentiate treatment may reduce pressure overuse.

Язык: Английский

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Genomic and Metabolic Characteristics of the Pathogenicity in Pseudomonas aeruginosa

International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(23), С. 12892 - 12892

Опубликована: Ноя. 29, 2021

In recent years, the effectiveness of antimicrobials in treatment Pseudomonas aeruginosa infections has gradually decreased. This pathogen can be observed several clinical cases, such as pneumonia, urinary tract infections, sepsis, immunocompromised hosts, neutropenic cancer, burns, and AIDS patients. Furthermore, causes diseases both livestock pets. The highly flexible versatile genome P. allows it to have a high rate pathogenicity. numerous secreted virulence factors, resulting from its secretion systems, multi-resistance different classes antibiotics, ability produce biofilms are pathogenicity factors that cause problems fight against must better understood for an effective treatment. Infections by represent, therefore, major health problem and, resistance genes disseminated between microbiotas associated with humans, animals, environment, this issue needs addressed on basis One Health approach. review intends bring together describe detail molecular metabolic pathways aeruginosa's pathogenesis, contribute development more targeted therapy pathogen.

Язык: Английский

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Skin manifestations of Pseudomonas aeruginosa infections

Current Opinion in Infectious Diseases, Год журнала: 2021, Номер 34(2), С. 72 - 79

Опубликована: Янв. 21, 2021

Purpose of review Pseudomonas aeruginosa is an opportunistic pathogen with considerable morbidity and mortality, particularly in vulnerable hosts. Skin manifestations are common, either representing local inoculation or secondary skin seeding following bloodstream infections. As patients various predisposing conditions expanding, we sought to the most recent published evidence regarding epidemiology, risk factors diagnosis P. . Recent findings New data exist on epidemiology infections; systemic infections impacted by multidrug-resistance issues host immune status. Summary Green nail syndrome, toe web infection, hot tub folliculitis, hand-foot infection external otitis common originating from per se. Local treatments cornerstone prognosis favorable immunocompetent Ecthyma gangrenosum subcutaneous nodules usually associated occur primarily immunocompromised Necrotizing soft tissue diabetic, alcoholic patients; management requires a multidisciplinary team surgical approach. Burn wound may also be challenging, requiring specialized team. In all four latter types portending significant antibiotics integral part treatment.

Язык: Английский

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Pseudomonas aeruginosa: an antibiotic resilient pathogen with environmental origin

Current Opinion in Microbiology, Год журнала: 2021, Номер 64, С. 125 - 132

Опубликована: Окт. 26, 2021

Pseudomonas aeruginosa, a bacterium characterized for its low antibiotics' susceptibility, is one of the most relevant opportunistic pathogens, causing infections at hospitals and in cystic fibrosis patients. Besides relevance human health, P. aeruginosa colonizes environmental ecosystems; therefore elements driving infectivity antibiotic resistance must be analyzed from One-Health perspective. Although some epidemic clones have been described, there are not specific lineages linked to infections, suggesting that virulence determinants evolved nature play functions other than infecting host avoiding antimicrobial treatment. Herein, we review current information on population structure functional role non-clinical ecosystems.

Язык: Английский

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A Systematic Review of Culture-Based Methods for Monitoring Antibiotic-Resistant Acinetobacter, Aeromonas, and Pseudomonas as Environmentally Relevant Pathogens in Wastewater and Surface Water

Current Environmental Health Reports, Год журнала: 2023, Номер 10(2), С. 154 - 171

Опубликована: Фев. 23, 2023

Abstract Purpose of Review Mounting evidence indicates that habitats such as wastewater and environmental waters are pathways for the spread antibiotic-resistant bacteria (ARB) mobile antibiotic resistance genes (ARGs). We identified members genera Acinetobacter , Aeromonas Pseudomonas key opportunistic pathogens grow or persist in built (e.g., wastewater) natural aquatic environments. Effective methods monitoring these ARB environment needed to understand their influence on dissemination ARGs, but standard have not been developed. This systematic review considers peer-reviewed papers where above were cultured from surface water, focusing accuracy current methodologies. Recent Findings studies suggest many clinically important ARGs originally acquired microorganisms. Aeromonas, species interest because ability provides opportunities engage horizontal gene transfer with other bacteria. Pathogenic strains organisms resistant multiple, relevant drug classes an urgent threat. However, culture generally developed clinical samples well-vetted samples. Summary The search criteria yielded 60 articles over past 20 years, which reported a wide variety isolation, confirmation, assays. Based comparison methods, we path forward standardizing methodologies water

Язык: Английский

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