Characterization of the single cell landscape in normal and osteoarthritic equine joints DOI Open Access
Dylan T. Ammons, Lyndah Chow, Laurie R. Goodrich

и другие.

Annals of Translational Medicine, Год журнала: 2024, Номер 12(5), С. 88 - 88

Опубликована: Окт. 1, 2024

Osteoarthritis (OA) is a major source of pain and disability worldwide. Understanding disease progression evolving, but OA increasingly thought to be multifactorial in which the innate immune system plays role regulating perpetuating low-grade inflammation. The aim this study was enhance our understanding immunopathogenesis through characterization transcriptomic responses joints, with goal facilitate development targeted therapies.

Язык: Английский

Single-cell transcriptomics reveals novel chondrocyte and osteoblast subtypes and their role in knee osteoarthritis pathogenesis DOI Creative Commons
Yuan Liu, Wacili Da,

Mingjie Xu

и другие.

Signal Transduction and Targeted Therapy, Год журнала: 2025, Номер 10(1)

Опубликована: Фев. 4, 2025

Abstract Research on treating knee osteoarthritis (KOA) is becoming more challenging due to a growing number of younger patients being affected. The pathogenesis KOA complex for multifactorial disease affecting the entire joint, with remodeling subchondral bone playing key role in degeneration overlying cartilage. Therefore, this study constructed bipedal postmenopausal mouse model better understand how interplay between and cartilage contributes development. A single-cell atlas osteochondral composite tissue was established. Furthermore, three novel subtypes chondrocytes, including Smoc2 + angiogenic Angptl7 Col1a1 osteogenic were identified femoral condyles mice. In addition, chondrocytes promoted angiogenesis mice by interacting endothelial cells via FGF2-FGFR2 signaling pathway. H-type vessels increased bone, recruiting osteoprogenitor facilitating osteogenesis Sparc osteoblasts have negatively regulated mineralization osteoblastic differentiation, aggravated pathological progression KOA. above findings offered new targets opened up an avenue therapeutic intervention

Язык: Английский

Процитировано

1
Circulating miR-126-3p is a mechanistic biomarker for knee osteoarthritis DOI Creative Commons
Thomas G Wilson, Madhu Baghel,

Navdeep Kaur

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 27, 2025

Язык: Английский

Процитировано

1
Single‐cell RNA sequencing in osteoarthritis DOI Creative Commons

Yuyuan Gu,

Yan Hu,

Hao Zhang

и другие.

Cell Proliferation, Год журнала: 2023, Номер 56(12)

Опубликована: Июнь 14, 2023

Osteoarthritis is a progressive and heterogeneous joint disease with complex pathogenesis. The various phenotypes associated each patient suggest that better subgrouping of tissues genotypes in different phases osteoarthritis may provide new insights into the onset progression disease. Recently, single-cell RNA sequencing was used to describe pathogenesis on high-resolution view surpassing traditional technologies. Herein, this review summarizes microstructural changes articular cartilage, meniscus, synovium subchondral bone are mainly due crosstalk amongst chondrocytes, osteoblasts, fibroblasts endothelial cells during progression. Next, we focus promising targets discovered by its potential applications target drugs tissue engineering. Additionally, limited amount research evaluation bone-related biomaterials reviewed. Based pre-clinical findings, elaborate clinical values for therapeutic strategies osteoarthritis. Finally, perspective future development patient-centred medicine therapy combining other multi-omics technologies discussed. This will cellular level field personalized therapeutics future.

Язык: Английский

Процитировано

20
Signalling interaction between β‐catenin and other signalling molecules during osteoarthritis development DOI Creative Commons
Jing Feng, Qing Zhang, Feifei Pu

и другие.

Cell Proliferation, Год журнала: 2024, Номер 57(6)

Опубликована: Янв. 10, 2024

Abstract Osteoarthritis (OA) is the most prevalent disorder of synovial joint affecting multiple joints. In past decade, we have witnessed conceptual switch OA pathogenesis from a ‘wear and tear’ disease to entire joint. Extensive studies been conducted understand underlying mechanisms using genetic mouse models ex vivo tissues derived individuals with OA. These revealed that signalling pathways are involved in development, including canonical Wnt/β‐catenin its interaction other pathways, such as transforming growth factor β (TGF‐β), bone morphogenic protein (BMP), Indian Hedgehog (Ihh), nuclear κB (NF‐κB), fibroblast (FGF), Notch. The identification currently underway specific molecule(s) key pathway(s) playing decisive role development need be evaluated. This review will focus on recent progresses understanding critical β‐catenin TGF‐β, BMP, Notch, Ihh, NF‐κB, FGF. Understanding these novel insights into integration complex gene regulatory network during help us identify pathway leading discovery therapeutic strategies for intervention.

Язык: Английский

Процитировано

9
Advancing skeletal health and disease research with single-cell RNA sequencing DOI Creative Commons
Peng Lin, Yibo Gan,

Jian He

и другие.

Military Medical Research, Год журнала: 2024, Номер 11(1)

Опубликована: Май 30, 2024

Abstract Orthopedic conditions have emerged as global health concerns, impacting approximately 1.7 billion individuals worldwide. However, the limited understanding of underlying pathological processes at cellular and molecular level has hindered development comprehensive treatment options for these disorders. The advent single-cell RNA sequencing (scRNA-seq) technology revolutionized biomedical research by enabling detailed examination diversity. Nevertheless, investigating mechanisms in highly mineralized skeletal tissue poses technical challenges. In this review, we present a streamlined approach to obtaining high-quality single cells from provide an overview existing scRNA-seq technologies employed studies along with practical bioinformatic analysis pipelines. By utilizing methodologies, crucial insights into developmental dynamics, maintenance homeostasis, involved spine, joint, bone, muscle, tendon disorders been uncovered. Specifically focusing on joint diseases degenerative disc disease, osteoarthritis, rheumatoid arthritis using provided novel more nuanced comprehension. These findings paved way discovering therapeutic targets that offer potential benefits patients suffering diverse

Язык: Английский

Процитировано

9
Progress in multi-omics studies of osteoarthritis DOI Creative Commons
Yuanyuan Wei, Qian He, Xiaoyu Zhang

и другие.

Biomarker Research, Год журнала: 2025, Номер 13(1)

Опубликована: Фев. 11, 2025

Язык: Английский

Процитировано

1
Cyaonoside A-loaded composite hydrogel microspheres to treat osteoarthritis by relieving chondrocyte inflammation DOI Creative Commons

Xingyan An,

Fengjin Zhou,

Guangfeng Li

и другие.

Journal of Materials Chemistry B, Год журнала: 2024, Номер 12(17), С. 4148 - 4161

Опубликована: Янв. 1, 2024

Cyaonoside A (CyA), derived from the natural Chinese medicine, Cyathula officinalis Kuan, which was for a long time used to treat knee injuries and relieve joint pain in traditional showed an unclear mechanism protecting cartilage. In addition, CyA poorly hydrosoluble incapable of being injected directly into cavity, limited its clinical application. This study reveals that resisted IL-1β-mediated chondrogenic inflammation apoptosis. Next, transcriptome sequencing is explore potential mechanisms underlying regulation MSC differentiation. Based on these findings, CyA-loaded composite hydrogel microspheres (HLC) were developed they possessed satisfactory loading efficiency, suitable degradation rate good biocompatibility. HLC increased anabolic gene (Acan, COL2A, SOX9) expression, while downregulating expression catabolic marker MMP13 vitro. osteoarthritis mouse model, demonstrated promising therapeutic capabilities by integrity articular conclusion, this provides insights regulatory chondrocytes proposes microsphere-based advanced strategy osteoarthritis.

Язык: Английский

Процитировано

6
Single cell transcriptome profiling of infrapatellar fat pad highlights the role of interstitial inflammatory fibroblasts in osteoarthritis DOI Creative Commons
Hongxu Pu,

Chenghao Gao,

Yi Zou

и другие.

International Immunopharmacology, Год журнала: 2024, Номер 131, С. 111888 - 111888

Опубликована: Март 23, 2024

Osteoarthritis (OA) is a whole-joint disease in which the role of infrapatellar fat pad (IFP) its pathogenesis unclear. Our study explored cellular heterogeneity IFP to understand OA and identify therapeutic targets. Single-cell single-nuclei RNA sequencing were used analyze 10 samples, comprising 5 from patients healthy controls. Analyses included differential gene expression, enrichment, pseudotime trajectory, communication, along with comparative studies visceral subcutaneous fats. Key subcluster pathways validated using multiplex immunohistochemistry. The scRNA-seq performed on IFPs control group profiled expressions over 49,674 cells belonging 11 major cell types. We discovered that adipose stem progenitor (ASPCs), contributing formation both adipocytes synovial-lining fibroblasts (SLF). Interstitial inflammatory (iiFBs) ASPCs exhibit notable pro-inflammatory proliferative characteristics. identified four adipocyte subtypes, one subtype showing reduced lipid synthesis ability. Furthermore, iiFBs modulated activities macrophages T IFP. Compared tissues, represented distinctive subpopulation regulated cartilage proliferation through MK pathway. This presents comprehensive single-cell transcriptomic atlas IFP, uncovering complex landscape potential impact progression. findings highlight OA, especially pathway, opening new avenues for understanding developing novel targeted therapies.

Язык: Английский

Процитировано

3
Bridging relevance between microplastics, human health and bone metabolism: Emerging threats and research directions DOI Creative Commons
Yuan‐Wei Zhang, Dongyang Zhou, Sicheng Wang

и другие.

Environmental Chemistry and Ecotoxicology, Год журнала: 2024, Номер 6, С. 422 - 435

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

3
Identification and Validation of Pivotal Genes in Osteoarthritis Combined with WGCNA Analysis DOI Creative Commons
Chun Yang, Xinhua Chen, Jin Liu

и другие.

Journal of Inflammation Research, Год журнала: 2025, Номер Volume 18, С. 1459 - 1470

Опубликована: Янв. 1, 2025

The prevalence of osteoarthritis (OA), the most common chronic joint condition, is increasing due to aging population and escalating obesity rates, leading a significant impact on human health well-being. Thus, analyzing key targets OA through bioinformatics can help discover new biomarkers improve its diagnosis. microarray RNA-seq results were screened from Gene Expression Omnibus (GEO) database. Functional enrichment analyses, protein-protein interaction (PPI) analysis, weighted gene co-expression network analysis (WGCNA) DEGs performed. RT-qPCR WB further performed verify hub expression in rat. In this study, 35 genes identified differential using GSE169077 GSE114007 datasets. Enrichment revealed that these predominantly enriched HIF-1 signaling pathway, ECM-receptor interaction, FoxO pathway. Through integration validation animal models ROC curve four pivotal (GADD45B, CLDN5, HILPDA CDKN1B) finally identified. conclusion, could serve as novel for predicting treating OA, offering fresh insights into etiology pathogenesis.

Язык: Английский

Процитировано

0