Carrier Erythrocytes: An Overview

Drug Delivery, Год журнала: 2003, Номер 10(1), С. 9 - 20

Опубликована: Янв. 1, 2003

Application of erythrocytes, the most abundant cells human body with desirable physiologic and morphologic characteristics, in drug delivery has been exploited extensively. These cellular carriers, having remarkable biocompatibility, biodegradability, life-span circulation, can be loaded by a wide spectrum compounds therapeutic value using different chemically, as well physically, based methods. Most characteristics including shape, membrane fragility, deformability, hematologic indices undergo some degree irreversible changes during loading procedure. The efflux pattern encapsulated from carrier erythrocytes covers range between relatively rapid release (complete within few hours) no detectable until cell lysis. A series methods have tested successfully for improvement vitro storability without any significant biology efficacy. Carrier several potential applications, intravenous slow agents, enzyme therapy, targeting to reticuloendothelial system (RES), oxygen tissues, preparation fused cells.

Язык: Английский

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Drug, enzyme and peptide delivery using erythrocytes as carriers

Journal of Controlled Release, Год журнала: 2004, Номер 95(1), С. 27 - 49

Опубликована: Фев. 1, 2004

Язык: Английский

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Applications of carrier erythrocytes in delivery of biopharmaceuticals

Journal of Controlled Release, Год журнала: 2006, Номер 118(2), С. 145 - 160

Опубликована: Дек. 16, 2006

Язык: Английский

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Erythrocytes as Carriers: From Drug Delivery to Biosensors

Pharmaceutics, Год журнала: 2020, Номер 12(3), С. 276 - 276

Опубликована: Март 18, 2020

Drug delivery using natural biological carriers, especially erythrocytes, is a rapidly developing field. Such erythrocytes can act as carriers that prolong the drug's action due to its gradual release from carrier; bioreactors with encapsulated enzymes performing necessary reactions, while remaining inaccessible immune system and plasma proteases; or tool for targeted drug target organs, primarily cells of reticuloendothelial system, liver spleen. To date, have been studied wide range drugs, such enzymes, antibiotics, anti-inflammatory, antiviral etc., diagnostic purposes (e.g. magnetic resonance imaging). The review focuses only on drugs loaded inside defines main lines research bioactive substances, well advantages limitations their application. Particular attention paid in vivo studies, opening-up potential clinical use into erythrocytes.

Язык: Английский

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Influence of the glycocalyx and plasma membrane composition on amphiphilic gold nanoparticle association with erythrocytes

Nanoscale, Год журнала: 2015, Номер 7(26), С. 11420 - 11432

Опубликована: Янв. 1, 2015

Erythrocytes are attractive as potential cell-based drug carriers because of their abundance and long lifespan in vivo. Existing methods for loading cargos into erythrocytes include hypotonic treatments, electroporation, covalent attachment onto the membrane, all which require ex vivo manipulation. Here, we characterized properties amphiphilic gold nanoparticles (amph-AuNPs), comprised a ∼2.3 nm core an ligand shell, able to embed spontaneously within erythrocyte membranes might provide means load drugs red blood cells (RBCs) directly Particle interaction with RBC occurred rapidly at physiological temperature. We further show that amph-AuNP uptake by RBCs was limited glycocalyx particularly influenced sialic acids on cell surface proteoglycans. Using reductionist model membrane system synthetic lipid vesicles, confirmed importance fluidity regulating amph-AuNP/membrane interactions. These results thus evidence amph-AuNPs identify key components govern this interaction, providing framework development amph-AuNP-carrying 'pharmacytes'

Язык: Английский

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Factors associated with the performance of carrier erythrocytes obtained by hypotonic dialysis

Blood Cells Molecules and Diseases, Год журнала: 2004, Номер 33(2), С. 132 - 140

Опубликована: Авг. 10, 2004

Язык: Английский

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Erythrocytes As a Novel Delivery Vehicle for Biologics: From Enzymes to Nucleic acid-based Therapeutics

Therapeutic Delivery, Год журнала: 2012, Номер 3(3), С. 405 - 414

Опубликована: Март 1, 2012

Biological drugs are among the most exciting of future, offering better treatment options for patients than ever before but they need an appropriate delivery vehicle. Carrier erythrocytes one promising drug-delivery systems. Application as containers various minimizes toxicity, decreasing risk side effects and pathologic immune reactions against encapsulated agents well improving their efficacy, leading to patient compliance. This review discusses rationale use a vehicle biopharmaceuticals summarizes categories these new encapsulable compounds that currently under investigation. The authors' intent is describe development this system give reader overview remarkable potential naturally designed carriers versatility in field biologics pathological conditions.

Язык: Английский

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Recent advancements in erythrocytes, platelets, and albumin as delivery systems

OncoTargets and Therapy, Год журнала: 2016, Номер unknown, С. 2873 - 2873

Опубликована: Май 1, 2016

In the past few years, nanomaterial-based drug delivery systems have been applied to enhance efficacy of therapeutics and alleviate negative effects through controlled targeting releasing agents. However, carriers can achieve high efficacy, even when modalities surface markers are introduced. Immunological problems also limited their wide applications. Biological systems, such as erythrocytes, platelets, albumin, extensively investigated because unique properties. this review, albumin described efficient systems. Their properties, applications, advantages, limitations in disease treatment explained. This review confirms that these be used facilitate a specific, biocompatible, smart delivery.

Язык: Английский

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Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study

International Journal of Medical Sciences, Год журнала: 2011, Номер 8(3), С. 222 - 230

Опубликована: Янв. 1, 2011

Drug delivery systems including chemical, physical and biological agents that enhance the bioavailability, improve pharmacokinetics reduce toxicities of drugs.Carrier erythrocytes are one most promising drug investigated in recent decades.The bioavailability statin drugs is low due effects P-glycoprotein gastro-intestinal tract as well first-pass metabolism.Therefore this work we study effect time, temperature concentration on loading pravastatin human to be using them systemic sustained release system for drug.After process performed carriers' were physically cellulary characterized.Also, vitro from carrier was studied over time interval.Our results revealed that, have been successfully loaded with endocytosis method either at 25 o C or 37 C. The amount 10 mg/ml 0.32mg/0.1 ml 0.69 mg/0.1 ml.Entrapment efficiency 34% 94% respectively 4 mg/ml.Moreover percent cells recovery 87-93%.Hematological parameters osmotic fragility behavior similar native erythrocytes.Scanning electron microscopy demonstrated has no change morphology.Pravastatin releasing cell 83% after 23 hours phosphate buffer saline decreased 72% by treatment glutaraldehyde.The pattern obeyed first order kinetics.It concluded entrapped into acceptable moderate morphological changes, suggesting can used prolonged pravastatin.

Язык: Английский

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Preparation and in-vitro characterization of tramadol-loaded carrier erythrocytes for long-term intravenous delivery

Journal of Pharmacy and Pharmacology, Год журнала: 2010, Номер 63(3), С. 322 - 332

Опубликована: Дек. 22, 2010

The hypo-osmotic dialysis method was used for preparation of tramadol-loaded human intact erythrocytes. In response to rapid drug escape from the erythrocytes, a membrane cross-linker, glutaraldehyde, successfully.The resulting carrier cells were validated in terms accuracy and precision whole loading procedure.The average loaded amount, entrapment efficiency cell recovery 1.9041 mg, 95.98% 85.13%, respectively. effects different concentrations on parameters studied with concentration 10 mg/ml selected as optimal. A series in-vitro characteristics including tramadol release behaviour, haematological indices, particle size distribution, scanning electron microscopy, osmotic/turbulence fragilities determined compared sham-entrapped unloaded cells. results these tests indicated that erythrocytes did not undergo remarkable irreversible shape/topology changes, but fragility membranes processed increased.The collective this study showed optimized suitable encapsulation final ready enter in-vivo animal studies promising long-circulating tramadol.

Язык: Английский

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