FTO aggravates podocyte injury and diabetic nephropathy progression via m6A-dependent stabilization of ACC1 mRNA and promoting fatty acid metabolism
Biochemical Pharmacology,
Год журнала:
2025,
Номер
235, С. 116819 - 116819
Опубликована: Фев. 21, 2025
Язык: Английский
Higher Circulating Neutrophil Counts Is Associated with Increased Risk of All-Cause Mortality and Cardiovascular Disease in Patients with Diabetic Kidney Disease
Biomedicines,
Год журнала:
2024,
Номер
12(8), С. 1907 - 1907
Опубликована: Авг. 20, 2024
Accumulating
evidence
has
suggested
the
pathogenic
roles
of
chronic
inflammation
and
neutrophils
in
diabetic
kidney
disease
(DKD).
This
study
investigated
relationship
between
neutrophils,
all-cause,
cardiovascular
(CVD)
mortality
type
2
diabetes
mellitus
(T2DM)
patients
with
DKD.
Язык: Английский
Label-free multimodal optical biopsy reveals biomolecular and morphological features of diabetic kidney tissue in 2D and 3D
Nature Communications,
Год журнала:
2025,
Номер
16(1)
Опубликована: Май 15, 2025
Abstract
Kidney
disease,
the
ninth
leading
cause
of
death
in
United
States,
suffers
from
poor
diagnostic
efficiency
(10%).
Traditional
biopsies
use
molecular
reagents
to
enhance
power
but
are
limited
by
overlapping
spatial
and
chromatic
signals,
product
quality
variability,
additional
processing.
To
address
these
challenges
without
disrupting
routine
diagnostics,
we
implement
label-free
imaging
modalities—stimulated
Raman
scattering
(SRS),
second
harmonic
generation
(SHG),
two-photon
fluorescence
(TPF)—within
a
single
setup.
We
identify
morphological,
lipidomic,
metabolic
biomarkers
control
diabetic
kidney
samples
at
subcellular
resolution.
Label-free
Stimulated
Histology
(SRH)
reveals
distinct
collagen
morphology,
mesangial-glomerular
volumes,
lipid
saturation,
redox
status,
lipid-protein
concentrations
previously
unrecognized
diseases.
Using
same
tissue
section
enhances
value
compromising
tissue.
These
multimodal
broadly
deepen
understanding
disease
progression
integrating
fibrotic,
data.
Язык: Английский
Single-nucleus RNA sequencing reveals ARHGAP28 expression of podocytes as a biomarker in human diabetic nephropathy
Open Medicine,
Год журнала:
2025,
Номер
20(1)
Опубликована: Янв. 1, 2025
Abstract
Introduction
Diabetic
kidney
disease
(DKD)
represents
serious
diabetes-associated
complications,
and
podocyte
loss
is
an
important
histologic
sign
of
DKD.
The
cellular
molecular
profiles
podocytes
in
DKD
have
yet
to
be
fully
elucidated.
Methods
This
study
analyzed
kidney-related
single-nucleus
RNA-seq
datasets
(GSE131882,
GSE121862,
GSE141115)
human
diabetic
glomeruli
transcriptome
profiling
(GSE30122).
ARHGAP28
expression
was
validated
by
western
blot
immunohistochemistry.
Results
In
tissues,
154
differentially
expressed
genes
(DEGs)
were
identified
podocytes,
which
enriched
biological
processes
related
nephron
development
extracellular
matrix–receptor
interactions.
Similarly,
the
mouse
kidney,
344
DEGs
found,
clustering
pathways
associated
with
renal
signaling
mechanisms
like
PI3K/Akt
(phosphatidylinositol-3
kinase/protein
kinase
B)
PPAR
(peroxisome
proliferator-activated
receptor).
glomeruli,
438
identified,
showing
significant
enrichment
nephropathy.
Venn
analysis
revealed
22
common
across
being
notably
overexpressed
podocytes.
nephropathy
model
using
db/db
mice
showed
that
significantly
upregulated
cortex
glomeruli.
vitro
studies
a
high-glucose
corroborated
these
findings.
Conclusions
Collectively,
this
provides
insight
into
function
diagnosis
indicates
potential
biomarker
Язык: Английский
Targeting lipid metabolic reprogramming to alleviate diabetic kidney disease: molecular insights and therapeutic strategies
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 25, 2025
Diabetic
kidney
disease
(DKD)
is
one
of
the
major
complications
diabetes,
and
its
pathological
progression
closely
associated
with
lipid
metabolic
reprogramming.
Under
diabetic
conditions,
renal
cells
undergo
significant
abnormalities,
including
increased
uptake,
impaired
fatty
acid
oxidation,
disrupted
cholesterol
efflux,
enhanced
catabolism,
as
adaptive
responses
to
stress.
These
changes
result
in
accumulation
lipids
such
free
acids,
diacylglycerol,
ceramides,
leading
lipotoxicity
that
triggers
inflammation
fibrosis.
Hypoxia
DKD
microenvironment
suppresses
oxidation
promotes
synthesis
through
HIF-1α
pathway,
while
chronic
exacerbates
disturbances
via
inflammatory
cytokines,
inflammasomes,
macrophage
polarization.
Targeting
metabolism
represents
a
promising
therapeutic
strategy
for
alleviating
DKD;
however,
further
clinical
translational
studies
are
warranted
validate
efficacy
safety
these
approaches.
Язык: Английский
Mechanism and Therapeutic Targets of Circulating Immune Cells in Diabetic Retinopathy
Pharmacological Research,
Год журнала:
2024,
Номер
210, С. 107505 - 107505
Опубликована: Ноя. 14, 2024
Diabetic
retinopathy
(DR)
continues
to
be
the
leading
cause
of
preventable
vision
loss
among
working-aged
adults,
marked
by
immune
dysregulation
within
retinal
microenvironment.
Typically,
retina
is
considered
as
an
immune-privileged
organ,
where
circulating
cells
are
restricted
from
entry
under
normal
conditions.
However,
during
progression
DR,
this
privilege
compromised
breach
barrier
and
infiltrate
retina.
Increasing
evidence
suggests
that
vascular
neuronal
degeneration
in
DR
largely
driven
infiltration
cells,
particularly
neutrophils,
monocyte-derived
macrophages,
lymphocytes.
This
review
delves
into
mechanisms
therapeutic
targets
associated
with
these
cell
populations
offering
a
promising
innovative
approach
managing
disease.
Язык: Английский
Association Between Weight-Adjusted Waist Index and Albuminuria in Type 2 Diabetes Mellitus in the Chinese Population
Diabetes Metabolic Syndrome and Obesity,
Год журнала:
2024,
Номер
Volume 17, С. 3585 - 3592
Опубликована: Сен. 1, 2024
This
study
aimed
to
investigate
the
relationship
between
weight-adjusted
waist
index
(WWI)
and
albuminuria
in
patients
with
type
2
diabetes
mellitus
(T2DM)
Chinese
population.
Язык: Английский
Label-Free Optical Biopsy Reveals Biomolecular and Morphological Features of Diabetic Kidney Tissue in 2D and 3D
Опубликована: Окт. 29, 2024
Abstract
Kidney
disease,
the
ninth
leading
cause
of
death
in
United
States,
has
one
poorest
diagnostic
efficiencies
only
10%
1
.
Conventional
methods
often
rely
on
light
microscopy
analysis
2D
fixed
tissue
sections
with
limited
molecular
insight
compared
to
omics
studies.
Targeting
multiple
features
a
biopsy
using
or
chemical
reagents
can
enhance
phenotyping
but
are
by
overlap
their
spatial
and
chromatic
properties,
variations
quality
products,
multimodal
nature
need
additional
processing.
To
overcome
these
limitations
increase
breadth
information
available
from
without
an
impact
routine
workup,
we
implemented
label-free
imaging
modalities
including
stimulated
Raman
scattering
(SRS)
microscopy,
second
harmonic
generation
(SHG),
two
photon
fluorescence
(TPF)
into
single
setup.
We
visualized
identified
morphological,
structural,
lipidomic,
metabolic
biomarkers
control
diabetic
human
kidney
samples
3D
at
subcellular
resolution.
The
biomarkers,
collagen
fiber
morphology,
mesangial-glomerular
fractional
volume,
lipid
saturation,
redox
status,
relative
protein
concentrations
form
Stimulated
Histology
(SRH),
illustrate
distinct
disease
tissues
not
previously
appreciated.
same
section
be
used
for
work
up
thus
enhancing
power
cliniopathological
insights
obtainable
compromising
already
tissue.
metrics
broadly
applicable
deepen
our
understanding
progression
diseases
integrating
fibrotic,
data.
Figure
Graphical
Label-free
indicators
nephropathies.
Язык: Английский
Advances in Understanding Diabetic Kidney Disease Progression and the Mechanisms of Acupuncture Intervention
International Journal of General Medicine,
Год журнала:
2024,
Номер
Volume 17, С. 5593 - 5609
Опубликована: Ноя. 1, 2024
Diabetic
kidney
disease
(DKD)
is
a
form
of
damage
resulting
from
diabetes
and
represents
common,
severe
complication
the
disease.
Globally,
DKD
leading
cause
chronic
(CKD).
occurs
in
both
type
1
2
patients,
with
early
clinical
manifestations
including
microalbuminuria
hypertension.
As
condition
progresses,
symptoms
such
as
massive
proteinuria,
hypoalbuminemia,
edema
may
appear.
The
key
to
treating
lies
controlling
blood
glucose
pressure,
reducing
slowing
deterioration
function.
Acupuncture,
traditional
Chinese
medicine
treatment,
has
shown
some
therapeutic
effects
on
through
mechanisms
regulating
glucose,
improving
renal
flow
microcirculation,
antioxidation
anti-inflammation,
modulating
gut
microbiota.
Язык: Английский