Inflammasomes in Alveolar Bone Loss

Frontiers in Immunology, Год журнала: 2021, Номер 12

Опубликована: Июнь 9, 2021

Bone remodeling is tightly controlled by osteoclast-mediated bone resorption and osteoblast-mediated formation. Fine tuning of the osteoclast–osteoblast balance results in strict synchronization formation, which maintains structural integrity tissue homeostasis; contrast, dysregulated may cause pathological osteolysis, inflammation plays a vital role promoting destruction. The alveolar presents high turnover rate, complex associations with tooth periodontium, susceptibility to oral pathogenic insults mechanical stress, enhance its complexity host defense remodeling. Alveolar loss also involved systemic destruction affected medication or factors. Therefore, it essential investigate osteoimmunological mechanisms dysregulation inflammasome supramolecular protein assembled response pattern recognition receptors damage-associated molecular patterns, leading maturation secretion pro-inflammatory cytokines activation inflammatory responses. Pyroptosis downstream facilitates clearance intracellular pathogens irritants. However, inadequate excessive activity allow for persistent infection spreading uncontrolled bone, as commonly observed periodontitis, periapical peri-implantitis, orthodontic movement, medication-related osteonecrosis jaw, nonsterile sterile osteomyelitis osteoporosis. In this review, we present framework understanding mechanism canonical noncanonical inflammasomes pathogenesis development etiologically diverse diseases associated loss. Inappropriate drive osteolysis regulating cellular players, including osteoclasts, osteoblasts, osteocytes, periodontal ligament cells, macrophages, monocytes, neutrophils, adaptive immune such T helper 17 causing increased osteoclast activity, decreased osteoblast enhanced periodontium creating milieu context- cell type-dependent manner. We discuss promising therapeutic strategies targeting inappropriate treatment Novel inhibiting signaling facilitate versatile drugs that carefully beneficial contributions defense.

Язык: Английский

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Senescence-associated secretory phenotype and its impact on oral immune homeostasis

Frontiers in Immunology, Год журнала: 2022, Номер 13

Опубликована: Окт. 4, 2022

The senescence-associated secretory phenotype (SASP), which accumulates over the course of normal aging and in age-related diseases, is a crucial driver chronic inflammation phenotypes. It also responsible for pathogenesis multiple oral diseases. However, pathogenic mechanism underlying SASP has not yet been fully elucidated. Here, relevant articles on published last five years (2017–2022) were retrieved used bibliometric analysis, first time, to examine composition. More than half focus various cytokines (27.5%), growth factors (20.9%), proteases (20.9%). In addition, lipid metabolites (13.1%) extracellular vesicles (6.5%) have received increasing attention past years, recognized as novel categories. Based this, we summarize evidences demonstrating that plays pleiotropic role immunity propose four-step hypothetical framework progression SASP-related pathology—1) development, 2) pathological alterations, 3) changes leading immune homeostasis disruption, 4) SASP-mediated dysregulation escalating disease. By targeting specific factors, potential therapies can be developed treat

Язык: Английский

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Hyperglycemia Aggravates Periodontitis via Autophagy Impairment and ROS-Inflammasome-Mediated Macrophage Pyroptosis

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(7), С. 6309 - 6309

Опубликована: Март 27, 2023

Macrophage pyroptosis drives the secretion of IL-1β, which has been recently reported to be a featured salivary biomarker for discriminating periodontitis in presence diabetes. This study aimed explore whether macrophage plays role development diabetes mellitus-periodontitis, as well potential therapeutic strategies. By establishing model experimental mellitus-periodontitis rats, we found that IL-1β and gasdermin D were highly expressed, leading aggravated destruction periodontal tissue. MCC950, potent selective molecule inhibitor NLRP3 inflammasome, effectively inhibited attenuated alveolar bone losses mellitus-periodontitis. Consistently, vitro, high glucose could induce thus promoted production macrophages stimulated by lipopolysaccharide. In addition, autophagy blockade via mTOR-ULK1 pathway led severe oxidative stress response Activation rapamycin, clearance mitochondrial ROS mitoTEMPO, inhibition inflammasome MCC950 significantly reduce secretion. Our demonstrates hyperglycemia promotes suffered microbial stimuli. Modulation activity specific targeting ROS-inflammasome may offer promising strategies alleviate

Язык: Английский

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Mediterranean diet: a potential player in the link between oral microbiome and oral diseases

Journal of Oral Microbiology, Год журнала: 2024, Номер 16(1)

Опубликована: Март 19, 2024

Background The oral microbiome is a complex and dynamic assemblage of microorganisms that colonize different sites the cavity maintaining both systemic health. Therefore, when its composition altered, diseases occur. Among inflammatory pathologies, periodontal affect tissues surrounding teeth, representing main cause tooth loss one most important threats to Lifestyle eating habits influence human microbiota development progression diseases. In this context, Mediterranean Diet (MD) model, comprising healthy dietary choices lifestyle, linked prevention several metabolic chronic-degenerative pathological processes, including Indeed, MD plant-based diet, enriched anti-inflammatory antioxidant nutrients, which may induce beneficial effects against dental caries

Язык: Английский

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Realizing the clinical utility of saliva for monitoring oral diseases

Periodontology 2000, Год журнала: 2024, Номер 95(1), С. 203 - 219

Опубликована: Июнь 1, 2024

In the era of personalized/precision health care, additional effort is being expended to understand biology and molecular mechanisms disease processes. How these are affected by individual genetics, environmental exposures, behavioral choices will encompass an expanding role in future optimally preventing treating diseases. Considering saliva as important biological fluid for analysis inform oral detection/description continues expand. This review provides overview a diagnostic features various biomarkers that have been reported. We emphasize use salivary periodontitis transport reader through extant literature, gaps knowledge, structured approach toward validating determine utility periodontitis. A summation findings support likelihood panel including both host molecules specific microorganisms be required most effectively identify risk early transition disease, ongoing activity, progression, response standard periodontal therapy. The goals would develop predictive algorithms serve adjunctive tools which provide clinician patient information making informed clinical decisions.

Язык: Английский

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Active Matrix Metalloproteinase-8 (aMMP-8) Versus Total MMP-8 in Periodontal and Peri-Implant Disease Point-of-Care Diagnostics

Biomedicines, Год журнала: 2023, Номер 11(11), С. 2885 - 2885

Опубликована: Окт. 25, 2023

Active matrix metalloproteinase-8 (aMMP-8) is a promising biomarker candidate for the modern periodontal and peri-implant disease diagnostics utilizing chairside/point-of-care oral fluid technologies. These rapid analysis technologies utilize gingival crevicular (GCF), sulcular (PISF), or mouth rinse as matrices that can be collected patient-friendly non-invasively without causing bacteremia. aMMP-8, but not total latent proMMP-8, has been shown to relevant implemented latest 2017 classification system of periodontitis peri-implantitis. Thus, aMMP-8 point-of-care-testing (POCT)-but proMMP-8-can conveniently used an adjunctive preventive diagnostic tool identify screen developing ongoing breakdown well predict its episodic progression. Similarly, POCT provides important monitor treatment effect these diseases, also other diseases such head neck cancer, where it tissue destructive side-effects during after radiotherapy. Additionally, recent studies support benefitting identification diabetes escalating risk COVID-19 infection. Overall, launched new clinical field in medicine dentistry, i.e., chemistry.

Язык: Английский

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Plasmon resonance biosensor for interleukin-1β point-of-care determination: A tool for early periodontitis diagnosis

iScience, Год журнала: 2023, Номер 27(1), С. 108741 - 108741

Опубликована: Дек. 14, 2023

Among pro-inflammatory cytokines, Interleukin-1β is crucially involved in several inflammatory-based diseases and even cancer. Increased levels oral fluids have been proposed as an early marker of periodontitis, a broadly diffused chronic inflammatory condition periodontal-supporting tissues, leading eventually to tooth loss. We describe the development portable surface-plasmon-resonance-based optical fiber probe suitably coated with anti-Interleukin-1β antibody monolayer. A pico-nanomolar linear range determination was obtained both buffer solution saliva rapid (3 min) incubation high selectivity presence interferents. Higher concentration periodontitis patient compared healthy control determined. These measurements were validated by automated ELISA system. Our results sustain potential applicability SPR-POF diagnostic point-of-care device for real-time monitoring salivary Interleukin-1β, that can support detection pathologies timely therapeutic decisions.

Язык: Английский

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ROS-Induced Gingival Fibroblast Senescence: Implications in Exacerbating Inflammatory Responses in Periodontal Disease

Inflammation, Год журнала: 2024, Номер unknown

Опубликована: Апрель 17, 2024

Язык: Английский

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Biomarker panel discriminates diabetics with and without periodontitis pre‐ and post‐therapy

Journal of Periodontal Research, Год журнала: 2023, Номер 58(3), С. 493 - 502

Опубликована: Апрель 12, 2023

Biological regulators of periodontal inflammation, collagen degradation, and insulin resistance have not been determined in association with severity periodontitis response to treatment diabetics. Our objective was determine whether type 2 diabetes (T2DM) patients disease present a distinct salivary biomarker profile compared T2DM without healthy subjects (without periodontitis) pre- post-nonsurgical therapy.Clinical parameters health whole unstimulated saliva were collected from 92 participants (31 Not Periodontitis, NP; 32 periodontitis, DWoP; 29 DWP) at baseline. The groups received scaling root planning (SRP) provided 6-week follow-up. Salivary concentrations interleukin (IL)-1β, IL-6, matrix metalloproteinase-8 (MMP-8), resistin measured by immunoassay.The DWP group had significantly more higher baseline for IL-1β, MMP-8, (p's < .01) DWoP NP. SRP resulted significant improvement the groups; however, persisted (p .001), remained than 6 weeks post-treatment. Principal component analysis demonstrated appeared biologically similar NP group. Concentrations these biomarkers increased increasing .05) this study population.Salivary appear serve as status post-treatment, irrespective status.

Язык: Английский

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Salivary advanced glycated end products, their receptors, and aMMP‐8 in periodontitis patients with varying glycemic levels: A cross‐sectional study

Journal of Periodontology, Год журнала: 2024, Номер unknown

Опубликована: Дек. 4, 2024

Abstract Background Advanced glycation end products (AGE) and their receptors (RAGE) have been implicated in developing periodontal complications diabetic patients. This study aimed to identify salivary AGE, RAGE, soluble RAGE (sRAGE), active‐matrix metalloproteinase‐8 (aMMP‐8) levels at varying glycemic periodontitis Methods Ninety‐eight participants were categorized into uncontrolled DM‐PD group ( n = 27)—periodontitis patients with Type 2 diabetes mellitus (T2DM) (glycated hemoglobin [HbA1c] ≥7%); controlled 33)—periodontitis T2DM (HbA1c 5.7%–6.9%); SH‐PD 18)—systemically healthy patients; SH‐PH 20)—systemically periodontally individuals. HbA1c along the parameters bleeding on probing (BoP), depth (PPD), clinical attachment loss (CAL), number of missing teeth, inflamed surface area (PISA) estimated. Enzyme‐linked immunosorbent assay (ELISA) was used for analyzing sRAGE, aMMP‐8. Multiple linear regression analysis conducted develop predictive models based relevant predictor variables. Results Periodontitis exhibited significantly higher BoP, PPD, CAL, PISA, elevated aMMP‐8, compared other groups p < 0.01). A significant positive association observed between Among predictors, BoP 0.046) CAL 0.001) demonstrated a effect AGE. PPD positively associated 0.05), negatively sRAGE 0.038). Conclusions Salivary biomarkers like aMMP‐8 exert potential role monitoring health control Plain language summary Group 1 (T2DM); T2DM; 3 4 The revealed that severe increased parameters, systemically groups. Conversely, lower group. also found associations glycated (HbA1c), as well parameters. findings this highlight utility biomarkers, particularly noninvasive diagnostic tools evaluate individuals diabetes.

Язык: Английский

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