American Journal of Reproductive Immunology,
Год журнала:
2024,
Номер
91(3)
Опубликована: Март 1, 2024
Abstract
Background
Endometritis
is
an
inflammatory
reaction
of
the
lining
uterus,
leading
to
occurrence
infertility.
Platelet
rich
plasma
(PRP)
has
been
proven
exhibit
extremely
effective
for
treatment
endometrium‐associated
infertility,
but
mechanism
its
prevention
endometritis
remains
unclear.
Objective
The
present
study
aimed
investigate
protective
effect
PRP
against
induced
by
lipopolysaccharide
(LPS)
and
elucidate
underlying
these
effects.
Methods
Mouse
model
was
established
intrauterine
perfusion
LPS.
infusion
administered
at
24
h
after
LPS
induction.
After
another
h,
uterine
tissues
were
harvested
observe
histopathological
changes,
production
proinflammatory
cytokines,
variation
Toll‐like
receptor
4/nuclear
factor
κB
(TLR4/NF‐κB)
signaling
pathways,
validated
anti‐inflammatory
PRP.
myeloperoxidase
(MPO)
activity
concentration
nitric
oxide
(NO)
determined
using
assay
kit.
Proinflammatory
chemokines
(tumor
necrosis
factor‐α
(TNF‐α),
interleukin‐1β
(IL‐1β),
interleukin‐6
(IL‐6))
measured
ELISA
Real‐Time
PCR.
TLR4/NF‐κB
pathway
in
Western
blotting.
Results
Hematoxylin‐eosin
staining
(H&E)
appeared
that
remarkably
relieved
impairment
tissues.
Detection
MPO
NO
revealed
distinctly
mitigated
infiltration
cells
mice
with
significantly
affected
expression
TNF‐α,
IL‐1β,
IL‐6.
also
found
suppress
LPS‐induced
activation
pathway.
Conclusion
effectively
alleviates
via
restraining
signal
TLR4/NF‐κB.
These
findings
provide
a
solid
foundation
as
potential
therapeutic
agent
endometritis.
BMC Women s Health,
Год журнала:
2021,
Номер
21(1)
Опубликована: Март 4, 2021
Abstract
Background
The
diagnostic
criteria
of
chronic
endometritis
remain
controversial
in
the
treatment
for
infertile
patients.
Methods
A
prospective
observational
study
was
conducted
a
single
university
from
June
2014
to
September
2017.
Patients
who
underwent
frozen-thawed
blastocyst
transfer
with
hormone
replacement
cycle
after
histological
examination
presence
were
enrolled.
Four
used
define
according
number
plasma
cells
same
group
patients:
1
or
more
(≥
1)
cells,
2
2),
3
3),
5
5)
10
high-power
fields.
Pregnancy
rates,
live
birth
and
miscarriage
rates
non-chronic
groups
defined
each
criterion
calculated.
logistic
regression
analysis
performed
births
using
eight
explanatory
variables
(seven
infertility
factors
endometritis).
receiver
operating
characteristic
curve
drawn
optimal
cut-off
value
Results
total
69
patients
registered
53
finally
analyzed
exclusion.
When
designated
as
≥
cell
endometrial
stroma
per
fields,
pregnancy
rate,
rate
63.0%
vs.
30.8%,
51.9%
7.7%,
17.7%
75%
groups,
respectively.
This
resulted
highest
among
smallest
P
values
between
groups.
In
analysis,
an
variable
negatively
affecting
objective
only
when
diagnosed
obtained
one
found
fields
(sensitivity
87.5%,
specificity
64.9%).
Conclusions
Chronic
should
be
According
this
criterion,
adversely
affected
rate.
The Tohoku Journal of Experimental Medicine,
Год журнала:
2022,
Номер
258(3), С. 237 - 242
Опубликована: Янв. 1, 2022
Chronic
inflammation
in
cesarean
scar
defect
contributes
to
secondary
infertility
women
with
syndrom;
however,
it
remains
unclear
about
the
situation
of
uterine
cavity
syndrome.
This
ambidirectional
cohort
study
aimed
explore
effect
cavities
syndrome
on
at
a
single
university
hospital.
The
frequency
chronic
endometritis
infertile
patients
was
retrospectively
compared
between
group
and
non-cesarean
group.
endometriosis
also
investigated
who
underwent
laparoscopy.
level
tumor
necrosis
factor-α
interleukin-1β
prospectively
evaluated
history
section
(control
group)
using
an
enzyme-linked
immunosorbent
assay.
There
significant
difference
incidence
groups
(65.8%
46.0%,
respectively,
p
=
0.0315).
Endometriosis
detected
51
(70%)
Tumor
levels
were
significantly
higher
than
those
control
(p
0.0002
0.0217,
respectively).
Our
findings
suggest
that
one
cause
is
embryo
implantation
failure-associated
endometritis,
endometriosis,
cavity.
Reproductive Medicine and Biology,
Год журнала:
2023,
Номер
22(1)
Опубликована: Янв. 1, 2023
Abstract
Purpose
To
clarify
the
mechanisms
of
intrauterine
platelet‐rich
plasma
(PRP)
infusion
that
support
embryo
implantation
in
vitro
fertilization
treatment.
Methods
Blood
and
endometrial
samples
were
collected
from
four
infertile
women.
Human
stromal
cells
(HESCs)
cultured
passaged
equally
into
cell
culture
dishes
each
patient.
Two
treated
with
PRP
twice,
other
two
vehicle.
Subsequently,
cultures
without
decidualized
8‐bromoadenosine
3′,5′‐cyclic
AMP
progesterone
for
5
days.
Results
The
gene
expression
undifferentiated
or
HESCs
was
compared.
In
microarray
analysis,
381
63
differentially
expressed
genes
detected
HESCs,
respectively.
found
to
promote
associated
growth,
tissue
regeneration,
proinflammatory
response,
antibiotic
effects.
attenuate
involved
proliferation
inflammation
by
inhibiting
phosphoinositide
3‐kinase
signaling.
Conclusions
Platelet‐rich
regulates
reprogramming
depending
on
menstrual
cycle
phases
an
appropriate
manner,
suggesting
has
potential
increase
thickness
proliferative
phase
improve
immune
tolerance
secretory
phase.
BMC Women s Health,
Год журнала:
2023,
Номер
23(1)
Опубликована: Июнь 30, 2023
Abstract
Purpose
This
paper
was
mainly
conducted
to
investigate
the
effect
of
chronic
endometritis
(CE)
on
clinical
outcome
patients
with
unexplained
infertility.
Materials
and
methods
145
infertility
from
Reproductive
Center
our
hospital
January
2018
December
2021
were
selected
as
group.
42
definite
causes
control
group
during
same
period.
Both
groups
underwent
hysteroscopy
immunohistochemical
tests
for
CD38
CD138.
According
results
immunohistochemistry,
incidence
CE
between
two
analyzed.
Patients
accepted
oral
antibiotic
therapy
14
days.
Another
58
who
did
not
undergo
CD138
unexamined
expected
natural
pregnancy.
Follow-up
lasted
1
year,
pregnant
followed
up
until
delivery.The
pregnancy
rate,
spontaneous
abortion
rate
baby-carrying
home
compared.
Results
There
75
in
group,
prevalence
51.7%
(75/145).
Compared
(28.6%),
significantly
higher
(P
<
0.05).
After
treated
treatment,
patients’
61.3%
(46/75)
60%
(45/75)
which
than
those
group(43.1%
&
36.2%)
0.05),
while
2.2%
(1/46),which
lower
that
(16.0%)
Conclusions
For
infertility,
combined
endometrial
detection
should
be
performed
time
exclude
CE.
The
can
improved
by
treatment.
Australian and New Zealand Journal of Obstetrics and Gynaecology,
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 27, 2024
Part
II
of
the
Australasian
guideline
for
investigation
and
management
recurrent
pregnancy
loss
(RPL)
provides
evidence‐based
guidance
on
RPL
provided.
The
implications
inherited
acquired
thrombophilia
with
respect
to
suggestions
clinical
are
Autoimmune
factors,
including
human
leukocyte
antigen,
cytokines,
antinuclear
antibodies
coeliac
antibodies,
discussed.
Infective,
inflammatory
endometrial
causes
discussed
in
detail.
Environmental
lifestyle
male
factor
unexplained
outlined.
Levels
evidence
grades
consensus
provided
all
statements.
International Journal of Gynecological Pathology,
Год журнала:
2020,
Номер
40(6), С. 556 - 562
Опубликована: Дек. 14, 2020
While
acute
endometritis
is
a
reasonably
well-defined
entity
of
ascending
infection
and
attendant
active
inflammation,
chronic
less
well
defined.
As
part
broad
effort
to
define
refine
the
diagnostic
criteria
management
disease,
we
conducted
survey
pathologists
understand
variability
in
implications
diagnosis
nonspecific,
nonobstetric
endometritis.
Members
national
international
professional
pathology
societies
were
surveyed
utilizing
anonymous
electronic
surveys
designed
examine
criteria,
etiological
understanding
treatment
pathologic
There
was
substantial
among
used
for
making
endometritis,
with
28.5%
using
presence
single
plasma
cell
diagnosis.
additional
use
special
stains,
reporting
coexisting
lesions
hormonal
stage
endometrium.
no
differences
between
generalists
specialists
used,
except
significantly
greater
likelihood
gestational
The
pathologists,
including
gynecologic
has
potential
confound
patients.
Standardization
essential
American Journal of Reproductive Immunology,
Год журнала:
2020,
Номер
84(3)
Опубликована: Апрель 24, 2020
Chronic
endometritis
(CE)
is
an
unusual
inflammatory
condition
characterized
by
endometrial
plasmacyte
infiltration.
It
has
a
high
prevalence
in
women
with
reproductive
failure.
Because
of
its
characteristic
localization
patterns
and
molecular
functions,
syndecan-1
been
identified
as
biomarker
plasmacyte,
immunohistochemistry
(IHC)
becomes
the
most
dependable
diagnostic
method
for
CE.
In
this
review,
we
discuss
association
between
CE
failure,
clinicopathological
characterization
CE,
function
expression
syndecan-1,
progress
IHC
diagnosis
prediction
outcome.
Frontiers in Cell and Developmental Biology,
Год журнала:
2021,
Номер
9
Опубликована: Авг. 13, 2021
In
vitro
fertilization
(IVF)
is
an
important
assisted
reproductive
technology
in
treating
infertility,
whose
failure
rate
still
high.
Studies
suggested
that
uterine
microbiota
are
related
to
women’s
diseases
and
persisting
intrauterine
bacterial
infectious
conditions,
such
as
chronic
endometritis
(CE),
impairing
the
pregnant
processes.
However,
relationship
between
IVF
outcomes
open
question.
present
study,
94
patients
diagnosed
with
infertility
were
enrolled
divided
into
CE
(E
group,
n
=
25)
non-CE
(NE
69)
groups
depending
on
hysteroscopy
immunohistochemistry.
Subsequently,
E
(Ep,
8
Enp,
17)
NE
(NEp,
41
NEnp,
28)
pregnancy
non-pregnancy
outcomes,
respectively.
The
fluids
collected
microbial
profiles
examined
through
V4
region
of
16S
rRNA
gene
high-throughput
sequencing.
results
demonstrated
had
significantly
lower
clinical
compared
(32
vs.
58.42%,
p
0.0014).
relative
abundances
Proteobacteria
Acidobacteria
higher
whereas
high
Actinobacteria
Fusobacteria
observed
group
at
phylum
level.
At
genus
level,
Gardnerella
groups,
which
referred
negative
outcome.
conclusion,
may
be
a
key
factor
for
outcome
after
IVF,
plays
pivotal
role,
diversity
serve
biomarker
forecast
success
