BMC Oral Health, Год журнала: 2023, Номер 23(1)
Опубликована: Ноя. 19, 2023
To evaluate the effect of curcumin gel combined with scaling and root planing (SRP) on salivary procalcitonin in periodontitis treatment.
Язык: Английский
Clinical Science, Год журнала: 2024, Номер 138(12), С. 725 - 739
Опубликована: Июнь 1, 2024
Abstract Objectives: Clinical studies have confirmed that galectin-3 (Gal-3) levels are significantly elevated in periodontitis patients. The present study aimed to explore the effects of Gal-3 inhibition on periodontal inflammation vitro and vivo. Methods: Human gingival fibroblasts (HGFs) with or without knockdown were stimulated by lipopolysaccharide (LPS), a ligation-induced mouse model treated inhibitor was established. Hematoxylin–eosin (H&E) immunohistochemistry (IHC) staining used evaluate tissues. Quantitative real-time polymerase chain reaction (qRT-PCR) enzyme-linked immunosorbent assay (ELISA) detect Gal-3, interleukin (IL)-6, IL-8, C-C motif ligand 2 (CCL2) expression. Immunofluorescence western blotting NF-κB ERK signaling pathway activation. Micro-computed tomography analyse degree bone loss. Results: up-regulated inflamed tissues LPS-induced HGFs. markedly decreased IL-6, CCL2 expression blocked activation In model, alleviated IL-1β IL-6 infiltration tissue mitigated Conclusions: notably partly through blocking
Язык: Английский
Процитировано
5
PLoS ONE, Год журнала: 2024, Номер 19(7), С. e0297351 - e0297351
Опубликована: Июль 2, 2024
Objective This study aimed to evaluate the effect of antimicrobial photodynamic therapy (aPDT) and use probiotics on treatment halitosis. Methods Fifty-two participants, aged from 18 25 years, exhaling sulfhydride (H 2 S) ≥ 112 ppb were selected. They allocated into 4 groups (n = 13): Group 1: tongue scraper; 2: treated once with aPDT; 3: probiotic capsule containing Lactobacillus salivarius WB21 (6.7 x 10 8 CFU) xylitol (280mg), 3 times a day after meals, for 14 days; 4: aPDT days. Halimetry gas chromatography (clinical evaluation) microbiological samples collected dorsum before aPDT, as well 7, 14, 30 The clinical data failed follow normal distribution; therefore, comparisons made using Kruskal-Wallis test (independent measures) Friedman ANOVA (dependent followed by appropriate posthoc tests, when necessary. For data, seeing distribution, rank sum was performed Dunn’s post-test. significance level α 0.05. Results Clinical results (halimetry) showed an immediate significant reduction in halitosis (p 0.0008) and/or scraper 0.0006). Probiotics no difference relation initial levels 0.7530). No differences found control appointments. amount Porphyromonas gingivalis , Tannerella forsythia Treponema denticola not altered throughout analysis 0.1616, p 0.2829 0.2882, respectively). Conclusion There scraping, but there number bacteria study, or times, both results. New trials are necessary better assess tested therapies. Trial registration Trials NCT03996044 .
Язык: Английский
Процитировано
5
Polymers, Год журнала: 2021, Номер 13(13), С. 2114 - 2114
Опубликована: Июнь 28, 2021
Dermo-epidermal equivalents based on plasma-derived fibrin hydrogels have been extensively studied for skin engineering. However, they showed rapid degradation and contraction over time low mechanical properties which limit their reproducibility lifespan. In order to achieve better properties, elasticity biological we incorporated a elastin-like recombinamer (ELR) network, two types of ELR, one modified with azide (SKS-N3) other cyclooctyne (SKS-Cyclo) chemical groups at molar ratio 1:1 three different SKS (serine-lysine-serine sequence) concentrations (1, 3, 5 wt.%), into hydrogels. Our results decrease in gelation contraction, both the absence presence encapsulated human primary fibroblasts (hFBs), higher increase when SKSs content is equal or than 3%. hFBs proliferation an improvement lowest (1 wt.%) was used but started decreasing increasing concentration day 14 respect plasma control. Proliferation keratinocytes (hKCs) seeded top hybrid-plasma containing 1 3% no differences control hKCs observed wt.% SKS. These promising need balance between reduced indicate potential using this type hydrogel as testing platform pharmaceutical products cosmetics, future work will elucidate potential.
Язык: Английский
Процитировано
26
Clinical Oral Investigations, Год журнала: 2023, Номер 27(7), С. 3749 - 3758
Опубликована: Апрель 5, 2023
Язык: Английский
Процитировано
11
BMC Oral Health, Год журнала: 2023, Номер 23(1)
Опубликована: Авг. 21, 2023
Abstract Background During the last decades, in patients with periodontitis, periodontal treatment has been shown to reduce potential release of local and systemic biomarkers linked an early risk inflammatory disorders. This study evaluated efficacy non-surgical-periodontal (NSPT) on growth differentiation factor 15 (GDF-15) related circulating such as glutathione peroxidase 1 (GPx-1), c-reactive protein (hs-CRP), surfactant D (SP-D) explored whether subjects who had high GDF-15 levels at baseline showed increased clinical benefits following NSPT 6-months follow-up. Methods For this two-arm, parallel randomized trial, periodontitis were randomly allocated receive quadrant scaling root-planing (Q-SRP, n = 23, median age 51 years old) or full-mouth disinfection (FMD, 50 treatment. Clinical parameters recorded all enrolled patients. The primary outcome was analyse serum concentrations changes GPx-1, hs-CRP, SP-D 30, 90, 180-days follow-up after through enzyme-linked immunosorbent assay (ELISA) nephelometric techniques. Results In comparison FMD, Q-SRP group a significant improvement (p < 0.05) reduction mean 0.005), hs-CRP 0.001), 0.042) increase GPx-1 0.025) 6 months At treatment, there association between several GDF-15, 0.05 for parameters). Finally, ANOVA analysis revealed that, significantly impacted 0.015), 0.026) upregulation 0.045). Conclusion results evidenced both protocols improved analyzed biomarkers, but more efficacious than FMD approach. Moreover, presented benefited from 6-month Trial registration NCT05720481.
Язык: Английский
Процитировано
10
Heliyon, Год журнала: 2024, Номер 10(13), С. e34205 - e34205
Опубликована: Июль 1, 2024
Atrial fibrillation (AF) is the most common and clinically significant type of cardiac arrhythmia. Although catheter ablation (CA) can restore sinus rhythm in patients with AF, some experience recurrence after procedure. This requires us to find a simple effective way identify at high risk intervene early high-risk population improve patient prognosis. The mechanism AF unclear, but it involves several aspects including history, inflammation, myocardial fibrosis, genes. article summarizes current predictors CA, fibrosis markers, inflammatory MicroRNAs, Circular RNAs, scores, imaging indicators. Each predictor has its own scope application, predictive capacity joint application multiple may power. In addition, we summarize mechanisms involved recurrence. We hope that this review will assist researchers understand help them conduct further related studies.
Язык: Английский
Процитировано
4
BMC Oral Health, Год журнала: 2021, Номер 21(1)
Опубликована: Авг. 19, 2021
Abstract Background Probiotics affect both the development and stability of microbiota by altering colonization pathogens thus helps in stimulating immune system individual. The aim present study is to assess effect probiotics on peri-implantitis microflora, determining minimum inhibitory concentration (MIC) Lactobacillus reuteri, that can be effectively administered as an antimicrobial agent specific pathogens. Hence, this will helpful finding MIC L. Reuteri Methods This experimental research was conducted patients visiting periodontology department M. A. Rangoonwala college dental sciences centre. Sub-gingival plaque samples were collected from identify various microorganisms. identified microorganisms compared each other Chi-Square test used calculate statistical significance. isolated subjected probiotic reuteri in-vitro . Minimum assessed using serial dilution method. Results results showed presence Porphyromonas gingivalis , Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Streptococcus salivaris Staphylococcus aureus subgingival patients. Statistically, significantly higher proportion had When all affected L.reuteri except actinomycetemcomitans. Conclusion provides value for pathogen against L.reuteri. authors recommend avoid standard guidelines dose treatment peri-implant infections profile different periodontal pathogen.
Язык: Английский
Процитировано
23
BMC Oral Health, Год журнала: 2021, Номер 21(1)
Опубликована: Сен. 23, 2021
Abstract Background Although chronic periodontitis has previously been reported to be linked with Alzheimer's disease (AD), the pathogenesis between two is unclear. The purpose of this study analyze and screen relevant promising molecular markers (AD). Methods In paper, we analyzed three AD expression datasets extracted differentially expressed genes (DEGs), then intersected them obtained from text mining, finally integrated DEGs. We followed that by enriching matching cell signal cascade through DAVID analysis. Moreover, MCODE Cytoscape software was employed uncover protein–protein interaction (PPI) network hub gene. Finally, verified our data using a different independent cohort. Results gene set acquired abstracting groups, 12 common were obtained. Functional enrichment assessment uncovered cross-genes, which mainly morphogenesis involved in neuron differentiation, leading edge membrane, receptor ligand activity. After PPI creation, ten retrieved, consisting SPP1, THY1, CD44, ITGB1, HSPB3, CREB1, SST, UCHL1, CCL5 BMP7. function terms new dataset used verify previous dataset, found 22 GO one pathway, "ECM-receptor pathways", overlapping functional terms. Conclusions establishment above-mentioned candidate key genes, as well enriched signaling cascades, provides for periodontitis-related AD, may help diagnosis treatment patients future.
Язык: Английский
Процитировано
23
International Journal of Dentistry, Год журнала: 2021, Номер 2021, С. 1 - 7
Опубликована: Май 15, 2021
While periodontitis has been proven to have an impact on systemic conditions, such as cardiovascular diseases, pregnancy complications, or poor glycemic control in diabetic patients, the influence of peri-implantitis health not adequately explored literature yet. The existing evidence suggests that peri-implant lesions lead more intense inflammatory response than periodontitis. Given analogies between periodontal diseases and peri-implantitis, aim present paper was review scientific about potential correlation conditions. Two clinical trials animals reported experimental determined alteration hematological biological parameters. One human study risk indicators for found patients with had significantly higher levels triglyceride, uric acid, white blood cells lower vitamin D. It described affects through a number mechanisms, including increase mediators inflammation, which also role worsening patients. Similarly, may status cytokines IL-1, IL-6, IL-10 matrix metalloproteinases. microbiological mechanism, based dissemination bacteria, hypothesized complications. Again, it is plausible same could occur peri-implantitis. In conclusion, only few studies Although changes parameters, biochemical markers further are needed investigate this correlation.
Язык: Английский
Процитировано
21
BMC Medical Education, Год журнала: 2021, Номер 21(1)
Опубликована: Дек. 1, 2021
Язык: Английский
Процитировано
21