Current Microbiology, Год журнала: 2024, Номер 81(6)
Опубликована: Апрель 16, 2024
Язык: Английский
PLoS Genetics, Год журнала: 2025, Номер 21(1), С. e1011539 - e1011539
Опубликована: Янв. 21, 2025
Protein ubiquitination is usually coupled with proteasomal degradation and crucial in regulating protein quality. The E3 ubiquitin-protein ligase SCF (Skp1-Cullin-F-box) complex directly recognizes the target substrate via interaction between F-box substrate. determinant of specificity. limited number identified ubiquitin ligase-substrate pairs a major bottleneck field. Penicillium oxalicum contains many transcription factors, such as BrlA, CreA, XlnR, Ace1, conserved filamentous fungi that regulate fungal development (hemi)cellulase genes. Transcription factor Ace1 (also known SltA) positively correlated growth conidiation negatively expression A pair, Fbx23 -Ace1, P . Most PoFbx23 present free form within nucleus. small portion associates Skp1 to PoFbx23-Skp1 heterodimer or assembles three invariable core components (Skp1, Cul1, Rbx1) complex. Under glucose signal, absence (Δ fbx23 ) results decreased levels brlA gene which encodes master regulator for asexual six spore pigmentation genes ( abrB → abrA aygB arpA arpB albA encode proteins dihydroxynaphthalene-melanin pathway, along impaired conidiation. cellulose Δ mutant are significantly upregulated. When present, PoAce1 exists full-length version several low-molecular-weight degraded versions. has polyubiquitin modification. Deleting Po does not affect ace1 but remarkable accumulation all versions protein. Accumulated dysfunctional no longer binds promoters gene, including cellulase cbh1 eg1 , hemicellulase xyn11A pigmentation-related acts transcriptional coactivator, recognizing activating PoAce1, allowing latter different effects (activating repressing) under signals.
Язык: Английский
Процитировано
0
New Phytologist, Год журнала: 2025, Номер unknown
Опубликована: Янв. 30, 2025
Summary Fungal plant pathogens preferentially express a set of effector genes at specific infection stages to successfully colonize the host. However, it remains unclear how gene expression is regulated during host infection. This study identified Zn 2 Cys 6 transcription factor, TFV1 ( Transcription Factor for Virulence 1 ), whose deletion weakened virulence Colletotrichum orbiculare on its cucurbit hosts. The additional paralog gene, TVL1 ‐ like resulted in further reduction cucurbits. Notably, double mutants retained wild‐type Solanaceae Nicotiana benthamiana . Expression putative genes, including four host‐specific effectors protein infection, EPC1‐4 was commonly downregulated knockout mutants. Yeast one‐hybrid assays suggested that binds promoter regions EPC2 , EPC 3 and 4 indicating importance induced key Among effector‐like affected by deletion, novel LysM EPC5 as being specifically required Our extends knowledge regulatory mechanisms governing host‐ stage‐specific C.
Язык: Английский
Процитировано
0
Journal of Fungi, Год журнала: 2025, Номер 11(3), С. 223 - 223
Опубликована: Март 15, 2025
Transcription factors are extensively found in fungi and involved the regulation of multiple biological processes, including growth, development, conidiation, morphology, stresses tolerance, virulence, as well production secondary metabolites. Biocontrol is a complex process through which several biocontrol behaviors, such secretion cell wall-degrading enzymes metabolites, regulated by transcription factors. To date, biocontrol-related have been reported fungi, Beauveria bassiana, Clonostachys rosea, Coniothyrium minitans, different species genera Metarhizium, Trichoderma, Arthrobotrys. However, comprehensive reviews summarizing analyzing with potential these scarce. This review begins giving basic overview their functions. Then, role discussed. Lastly, possible approaches for further work on suggested. provides basis elaborating molecular mechanisms context biocontrol.
Язык: Английский
Процитировано
0
Plants, Год журнала: 2025, Номер 14(7), С. 985 - 985
Опубликована: Март 21, 2025
Plant-derived biopesticides are emerging as a promising and popular alternative for promoting cleaner safer agricultural practices. The present work aims to explore Argyranthemum frutescens (Asteraceae) source of botanical pesticides validate this through cultivation process. To task, bioassay-guided fractionation the ethanolic root extracts from both wild cultivated A. on phytopathogenic fungi Botrytis cinerea, Fusarium oxysporum, Alternaria alternata was conducted. This approach led identification polyacetylenes with higher potency than commercial fungicides. Specifically, compounds 3 (capillin) 5 (frutescinone) showed more 90% growth inhibition at 0.05 mg/mL concentration B. while 2 (capillinol) were also active positive controls, Fosbel-Plus Azoxystrobin, against F. oxysporum. structures isolated (1-6, 9, 10) alkamides (7, 8, 11) determined spectroscopic analysis, absolute configuration stereocenter C1 1, 2, 4 9 by NMR-spectroscopy (R)-(-)-α-methoxy-phenylacetic chiral derivatizing agent, biogenetic considerations. Overall, study supports potential agrochemical lead phytopathogens, validates viable biopesticides.
Язык: Английский
Процитировано
0
Molecular Plant Pathology, Год журнала: 2025, Номер 26(4)
Опубликована: Март 28, 2025
Several DNA-binding homeobox and different transcription factor (DDT)-domain proteins form stable remodelling complexes with imitation switch (ISWI) chromatin factors. ISWI have been reported to be involved in various biological processes many eukaryotic species. However, phytopathogenic fungi, the regulatory mechanisms underlying functions of DDT-domain remain unclear. Here, immunoprecipitation-sequencing (ChIP-seq) assays were used demonstrate that FgDDT from Fusarium graminearum was enriched within promoter regions genes associated metabolic MAPK signalling pathways, thereby activating their expression. Moreover, two additional genes, FgISW1 FgISW2, identified characterised, subsequent analyses indicating components are essential for fungal development pathogenicity rather than FgISW2. Further experiments revealed binds an complex activates expression functional F. graminearum, consequently contributing its development. also observed as highly conserved species but exhibits low similarity homologues Homo sapiens Arabidopsis thaliana, suggesting studies crucial uncover unique role Fusarium. These findings provide a basis further understanding molecular by which function fungi contribute pathogenicity.
Язык: Английский
Процитировано
0
Current Biology, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
Phytopathology Research, Год журнала: 2024, Номер 6(1)
Опубликована: Фев. 1, 2024
Abstract Rice blast is a serious threat to the safe production of grain crops such as rice and wheat. Sporulation, appressorium formation, invasive growth Magnaporthe oryzae are key stages development spread epidemics. M. hemibiotrophic fungus that undergoes changes in available carbon sources during infection cycle. Lipid major storage for spores source used glycerol synthesis turgor pressure generation appressoria. The formation dense cell wall melanin layer necessary an produce be pathogenic. plant important stage . Transcription factors regulate gene expression fungi intermediates between reception external environmental signals control pathogenicity disease cycle controlled by some transcription factors, sporulation Cos1 Hox2, Sfl1, Hox7, Vrf1, Mst12 Mig1, resistance host basal immunity Ap1 Atf1. This review focuses on describing sporulation, growth, lipid metabolism, carbohydrate synthesis, oxidative response, immunity, well working mechanism factors.
Язык: Английский
Процитировано
4
Journal of Fungi, Год журнала: 2024, Номер 10(4), С. 246 - 246
Опубликована: Март 25, 2024
Fusarium sacchari is a causal agent of sugarcane Pokkah boeng, an important fungal disease that causes considerable reduction in yield and sugar content susceptible varieties worldwide. Despite its importance, the factors regulate virulence this pathogen remain largely unknown. In our previous study, mapping insertional mutant defect resulted identification cutinase G-box binding protein gene, designated FsCGBP, encodes C2H2-type transcription factor (TF). FsCGBP was shown to localize nuclei, transcript level significantly upregulated during infection process or response abiotic stresses. Deletion silencing mycelial growth, conidial production, delay germination F. sacchari. Cutinase genes FsCUT2, FsCUT3, FsCUT4 mitogen-activated kinase (MAPK) FsHOG1, FsMGV1, FsGPMK1, which were downregulated ΔFsCGBP. Except for all these found be transcriptionally activated by using yeast one-hybrid system vitro. The deletion individual did not result any phenotypes exhibited ΔFsCGBP mutant, except activity. However, disruption MAPK pathway upon FsMGV1 FsGPMK1 similar those mutant. above results suggest functions regulating genes, providing new insights into mechanism regulation
Язык: Английский
Процитировано
4
Journal of Integrative Agriculture, Год журнала: 2024, Номер unknown
Опубликована: Апрель 1, 2024
Fusarium head blight (FHB), mainly caused by fungus graminearum (F. graminearum), is a devastating wheat disease worldwide, leading to reduced yield production and compromised grain quality due contamination mycotoxins, such as deoxynivalenol (DON). Manipulating the specific gene expression in microorganisms through RNA interference (RNAi) presents an opportunity for new-generation double-stranded (dsRNA)-based formulations combat large number of plant diseases. Here, we applied both spray-induced silencing (SIGS) host-induced (HIGS) target five virulence-related DON-synthesized genes F. graminearum, including protein kinase Gpmk1, zinc finger FgChy1, transcription factor FgSR, DON synthesis TRI5 cell-end marker FgTeaA, aiming effectively control FHB wheat. Direct spraying individual or combined siRNAs (small interfering RNA) from showed suppressed pathogenic symptoms during infection leaves, with combination all demonstrating superior resistance. Furthermore, generated transgenic lines expressing chimeric RNAi cassettes targeting these genes, two independent exhibited strong resistance crown rot, accumulation. Notably, HIGS did not adversely impact growth traits. Collectively, our findings support that SIGS represent effective strategies key bolstering crops.
Язык: Английский
Процитировано
4
Fungal Biology, Год журнала: 2025, Номер 129(3), С. 101565 - 101565
Опубликована: Март 9, 2025
Язык: Английский
Процитировано
0